Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,676,1060CA42.9% 174.2 / 51.0 105L240F (TTG→TTT) yphDputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (38/22);  new base A (0/45);  total (38/67)
Fisher's exact test for biased strand distribution p-value = 3.39e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAAC  >  NC_000913/2675957‑2676256
                                                                                                                                                        |                                                                                                                                                      
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCc‑aaca                                                                                                                                                       <  2:758626/151‑1 (MQ=255)
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCc‑aaca                                                                                                                                                       <  2:1861585/151‑1 (MQ=255)
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCc‑aac                                                                                                                                                        >  1:23017/1‑150 (MQ=255)
aGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCc‑aac                                                                                                                                                        >  2:1024401/1‑150 (MQ=255)
  cGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACaaa                                                                                                                                                     <  2:2080832/151‑1 (MQ=255)
  cGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACaaa                                                                                                                                                     <  2:129593/151‑1 (MQ=255)
   gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACaaa                                                                                                                                                     >  1:493974/1‑150 (MQ=255)
   gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACaaa                                                                                                                                                     >  2:1932361/1‑150 (MQ=255)
     aaTCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAAt                                                                                                                                                   <  1:2109299/150‑1 (MQ=255)
     aaTCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATg                                                                                                                                                  >  1:1982018/1‑151 (MQ=255)
        cACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGcc                                                                                                                                                <  1:555957/150‑1 (MQ=255)
        cACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑AAGGCGCGCCGCC‑AACAAAATGCCg                                                                                                                                               <  1:1704716/151‑1 (MQ=255)
         aCCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCgg                                                                                                                                              <  1:268689/151‑1 (MQ=255)
          ccAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCgg                                                                                                                                              <  1:666886/150‑1 (MQ=255)
          ccAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGt                                                                                                                                             <  2:1767408/151‑1 (MQ=255)
          ccAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGt                                                                                                                                             >  2:1656106/1‑151 (MQ=255)
                cacCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCAccg                                                                                                                                        <  1:964600/150‑1 (MQ=255)
                  ccAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCAccgccg                                                                                                                                     <  1:995222/151‑1 (MQ=255)
                  ccAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCAccgc                                                                                                                                       >  1:1675185/1‑149 (MQ=255)
                   cAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACcgccgc                                                                                                                                    >  2:1885952/1‑151 (MQ=255)
                   cAAGCAATGTACCGAACAGGAAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTCCCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCAccgccg                                                                                                                                     <  2:1123331/150‑1 (MQ=255)
                        aatgacccGAACGGGGAC‑CCGCGCCCGCGGGAAAGGGCCGTCCCGCCGACCACGACCGCGGCGATGACGTAAAACCCCAGCCCGTTTGCGGCCCCTGCGTTACCAAACCC‑GAGGCGCGCCGCC‑AAAAAAATGCCGGTCACCGCCGCtaata                                                                                                                               <  2:1725883/145‑1 (MQ=255)
                         aattcccGAAAAGGGAACCCCCGCCCCCCGGAAAGTCCCGTCCCGCCGCCCAGGCCCGCGGCGATGACGTCAACCTCCAGACCGTTTGCGCCCCCTGCGTTCCCAGAACC‑GGGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCta                                                                                                                                  <  2:597023/143‑1 (MQ=255)
                           gtacaaaaCAGGAAC‑CCGCGCCCGCCGAAAAGTCCCGTCCCGCCGACCCCGCCCGCGGCGATGACGTCAAACCCCAGACCTTTTGCGGCCCCTGCGTTCCCAGAACC‑GAGGCGCGCCGCA‑AAAAAAAGGCCGGTCACCGCCGCTAATAATc                                                                                                                            <  2:1908526/145‑1 (MQ=255)
                                 aaCGGGGAC‑CCGCGCCCGCCGGAAAGTGCCGTCCCGCCGACCACGACCGCGGCGATGACGTAAAACCCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGaaa                                                                                                                      <  1:1731347/151‑1 (MQ=255)
                                    aGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAggg                                                                                                                   >  2:763836/1‑151 (MQ=255)
                                     gggAA‑CCGCGCCCGCCGAAAAGTGCCGTCCCGCCGACCACGACCGCGGCGATGACGTAAAACTCCAGACCGTTTGCGGCCCCTGCGTTACAAGACCG‑GAGGCGCGCCGCC‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAg                                                                                                                     <  1:882871/148‑1 (MQ=255)
                                         acccGCGCCCGCCGGAAAGTGCCGTACCGCCGCCCACGACCGCGGCGATGACGTAAAACCCCAGCCCGTTTGCGGCCCCTGCGTTACCAGACCC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaaa                                                                                                               <  2:1828543/149‑1 (MQ=255)
                                         acccGCGCCCGCCGAAAAGTGCCGTCCCGCCGCCCACGACCGCGGCGATGACGTCAACCCCCAGACCTTTTGCGGCCCCTGCGTTACCAGAACC‑GAGCCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                                <  1:347884/148‑1 (MQ=255)
                                         acccGCGACCGCCGGAAAGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCTCCAGCCCGTTTGGGGCCCCTGCTTTACCAGAACC‑GGGGCGCGCCCCC‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAg                                                                                                                     <  1:1972926/143‑1 (MQ=255)
                                            cgcgACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTaa                                                                                                                >  1:394862/1‑147 (MQ=255)
                                              cgccccccgGAAAGGGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCCCCAGCCCGTTTGCGGCCCCTGCGTTACCAAACCC‑GAGGCGCGCCGCA‑AAAAAAATGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGAt                                                                                                            <  2:1392586/143‑1 (MQ=255)
                                                aCCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATa                                                                                                           >  1:2020606/1‑148 (MQ=255)
                                                 ccgcggAAAGGTGCCGTCCCGCCGCCCACGCCGGGGGCGATGACGTCAACCCCCAGCCCGTTTGGGGCACCTGCTTTCCAAAAACC‑GGGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGaa                                                                                                       <  2:1672858/151‑1 (MQ=255)
                                                 cccccgAAAAGTCCCGTACCCCCGACCACTCCCGCGGGGATGCCGTCAACCCCCAGACCGTTTGCGGCTCCTGCTTTCCCAGATCC‑GAGGCGCGCCGCA‑AACAAAGTGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAg                                                                                                         <  1:264137/146‑1 (MQ=255)
                                                     cgtaaaGGGCCCTACCGCCGACCACGCCGGGGGCGAGGACGACAACCTCCCGACCGTTTGCGGCACTTGCGTTCCGAAAACC‑GAGGCG‑GTCGCCAAACAAAGGCCCGGTCACCGCCGCTAATAATCCCGAGAGGGTAAAGAt                                                                                                            <  2:321529/139‑1 (MQ=255)
                                                      ggAAAGTGCCGTACCGCCGCCCACGCCCGCGGCGATGACGTAAAACCCCAGACCGTTTGCGGCCCCTGCGTTACAAAAACC‑GAGGCGCGCGGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt                                                                                                      <  2:69913/147‑1 (MQ=255)
                                                      gaaaaGTGCCGTCCCGCCGCCCCCGCCCGCGGCGATGACGTCAACCCCCAGCCCGTTTGCGGCCCCTGCTTTACCAGAACC‑GAGGCGCGCCGCA‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcg                                                                                                   <  2:127123/148‑1 (MQ=255)
                                                      gaaaaGCGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTCAACCCCCAGCCCGTTGGCGGCCCCGGCTTTCCAAGACCC‑GGGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgc                                                                                                    <  2:1841849/147‑1 (MQ=255)
                                                           gTGCCGTCCCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTCCCAGAACC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg                                                                                             <  1:1216356/151‑1 (MQ=255)
                                                              ccGTCCCGCCGCCCACGCCCGCGGCGATGACGTAAACCTCCAGACCTTTGGCGGCCCCTGCGTTACCAGAACC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg                                                                                          <  1:1792105/151‑1 (MQ=255)
                                                               cGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg                                                                                          >  1:1435340/1‑150 (MQ=255)
                                                                  aCCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACg                                                                                      >  2:4468/1‑151 (MQ=255)
                                                                   ccgccgCCCACGACCGCGGCGATGACTTCAACCTCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACC‑GAGGCGCGCCCCA‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACg                                                                                      <  1:1752111/150‑1 (MQ=255)
                                                                   ccgccgACCACGACCGCGGCGATGACGTCAACCCCCAGACCGTTTGCGGCCCCTGCGTTACCAGACCC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                                     <  1:1741008/151‑1 (MQ=255)
                                                                   ccgccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGt                                                                                     >  1:2127863/1‑151 (MQ=255)
                                                                     gccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTg                                                                                   >  2:1689058/1‑151 (MQ=255)
                                                                          ccACGCCCGCGGCGAGGACGTCAACCCCCAGACCGTTTGCGCCCCCGGCTTTCCCAGACCC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATg                                                                                <  1:1740331/149‑1 (MQ=255)
                                                                            aCGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGc                                                                            >  1:1795626/1‑151 (MQ=255)
                                                                                ccGCGGCGATGACGTCAACCCCCAGACCGTTTGCGGCCCCGGCGTTCCCAGAACG‑GGGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                          <  2:834827/149‑1 (MQ=255)
                                                                                  gcggcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt                                                                      >  1:1533358/1‑151 (MQ=255)
                                                                                    ggcgATGACGTCAAACCCCAGACCTTTTGCGGCCCCTGCTTTCCCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgc                                                                    <  1:393768/151‑1 (MQ=255)
                                                                                           aCGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGccgccg                                                             >  2:443150/1‑151 (MQ=255)
                                                                                              tCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGt                                                            >  2:1908380/1‑149 (MQ=255)
                                                                                                aacccccAGCCCGTTTGCGGCCCCTGCTTTCCCAGACCC‑GAGGCGCGCCGCC‑AAAAAAATGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAg                                                          <  1:45862/144‑1 (MQ=255)
                                                                                                aaaCCCCAGCCCTTTTGCGGCCCCTGCGTTCCCAGACCC‑GGGGCGCGCCGCC‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGcgccgc                                                               <  2:2075587/144‑1 (MQ=255)
                                                                                                aaaCCCCAGCCCGTTTGCGGCCCCTGCGTTACAAGAACG‑GGGGCGCGCCCCA‑AAAAAAATGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAg                                                          <  2:2149933/149‑1 (MQ=255)
                                                                                                 acccccAGCCCGTTTGCGGCCCCTGCTTCACCAAAACC‑GGGGCGCCCCGCA‑AAAAAAATGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAt                                                       <  2:1799603/147‑1 (MQ=255)
                                                                                                 aaCTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAt                                                       >  1:122708/1‑151 (MQ=255)
                                                                                                   ccccAGACCGTTGGCGCCCCGTGCG‑TACCAAAACCGGAGCCGCGCCCCA‑AAAAAAATCCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTa                                                     <  2:1819588/149‑1 (MQ=255)
                                                                                                   ccccAGACCGTTGGCGCCCCCTGCGTTACCAGAACC‑GAGGCGCGCCGCA‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTa                                                     <  2:1748111/149‑1 (MQ=255)
                                                                                                     ccAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAcc                                                   >  1:1609378/1‑151 (MQ=255)
                                                                                                         aCCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGcc                                                >  2:311016/1‑150 (MQ=255)
                                                                                                         aCCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGcc                                                >  2:969983/1‑150 (MQ=255)
                                                                                                          cttttggCGGCCCCGGCGTCACCAGACCG‑GGGGCGCCCCCCA‑AAAAAAAGGCGGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCa                                               <  2:641934/144‑1 (MQ=255)
                                                                                                          ccGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCa                                               >  2:225684/1‑150 (MQ=255)
                                                                                                             tttGCGGCCCCTGCGTTACCAGAACC‑GGGGCGCGCCGCC‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACg                                            <  1:1008188/150‑1 (MQ=255)
                                                                                                             tttGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACgg                                           >  1:651185/1‑151 (MQ=255)
                                                                                                                gCGGCCCCTGCGTTCCCAGAACC‑GAGGCGCGCCCCC‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCa                                         <  1:634442/150‑1 (MQ=255)
                                                                                                                 cGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaa                                        >  2:399693/1‑150 (MQ=255)
                                                                                                                  ggCCCCTGCGTTACCAGACCC‑GGGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaa                                       <  1:122668/150‑1 (MQ=255)
                                                                                                                  ggCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaa                                       >  1:671484/1‑150 (MQ=255)
                                                                                                                  ggCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaa                                       >  1:416581/1‑150 (MQ=255)
                                                                                                                    ccccggCGTTACCAAAACC‑GGGGCGCGCCGCC‑AAAAAAAGGCCGGTCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaaa                                      <  2:647747/144‑1 (MQ=255)
                                                                                                                     aCCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc                                   >  1:832475/1‑151 (MQ=255)
                                                                                                                      cctcctttcccaaaccc‑GAGGCGCCCCGCC‑AAAAAAATCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCg                                  <  1:1611609/136‑1 (MQ=255)
                                                                                                                      ccTGCTTTACCAAACCC‑GGGGCGCCCCGCA‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAc                                    <  2:1651999/149‑1 (MQ=255)
                                                                                                                      ccTGCGTTACCAGACCC‑GAGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAc                                    <  1:177366/149‑1 (MQ=255)
                                                                                                                            ttcccAGACCC‑GAGGCGCCCCCCA‑AAAAAAACGCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa                                 <  2:2126812/143‑1 (MQ=255)
                                                                                                                            ttACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGcc                            >  1:72970/1‑151 (MQ=255)
                                                                                                                               ccAGACCC‑GGGGCGCGCCGCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa                                 <  1:477825/143‑1 (MQ=255)
                                                                                                                                 aGAACC‑GGGCCGCGCCCCA‑AAAAAAAGGCCGGCCCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCg                          <  2:1027177/148‑1 (MQ=255)
                                                                                                                                  agaccggAGGCGCGCCGCC‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGaa                        <  1:1582395/144‑1 (MQ=255)
                                                                                                                                    acccgggccgcgcCCCA‑AAAAAAAGCCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAg                       <  2:468715/141‑1 (MQ=255)
                                                                                                                                    aCC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCg                    >  1:1365969/1‑151 (MQ=255)
                                                                                                                                        ggggCGCCCCGCA‑AAAAAAATGCCGGCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGt                  <  2:475318/148‑1 (MQ=255)
                                                                                                                                        gAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt                 >  2:1653504/1‑151 (MQ=255)
                                                                                                                                        gAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt                 >  2:1854133/1‑151 (MQ=255)
                                                                                                                                        gAGGCGCGCCCCA‑AAAAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCgg                   <  1:437007/149‑1 (MQ=255)
                                                                                                                                          ggCGCGCGGCA‑AAAAAAAGGCGGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtt                 <  1:830303/149‑1 (MQ=255)
                                                                                                                                          ggCGCGCCGCA‑AAAAAAAGCCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGttt                <  1:1914843/150‑1 (MQ=255)
                                                                                                                                            cgcgcCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc             >  2:1608457/1‑151 (MQ=255)
                                                                                                                                              cgccgcC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCgg           >  1:559188/1‑151 (MQ=255)
                                                                                                                                              cgccgcA‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGtttt               <  1:1321445/147‑1 (MQ=255)
                                                                                                                                               gccgcc‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGc          >  2:354468/1‑151 (MQ=255)
                                                                                                                                                 cgca‑AAAAAAAGGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCg            <  1:535799/147‑1 (MQ=255)
                                                                                                                                                   cc‑AAAAAAATCCCGATACCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCCCCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGc          <  2:1359132/147‑1 (MQ=255)
                                                                                                                                                      aacaaAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgatg     >  2:1548421/1‑150 (MQ=255)
                                                                                                                                                        caaAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAac  >  1:1534200/1‑151 (MQ=255)
                                                                                                                                                        caaAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAac  >  1:342132/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
AGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACC‑GAGGCGCGCCGCC‑AACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAAC  >  NC_000913/2675957‑2676256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑