Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 231,852 T→C V244A (GTA→GCA)  yafD → endo/exonuclease/phosphatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913231,8520TC99.0% 403.4 / NA 103V244A (GTA→GCA) yafDendo/exonuclease/phosphatase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (60/42);  minor base A (0/1);  total (60/43)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

AGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGA  >  NC_000913/231704‑231996
                                                                                                                                                    |                                                                                                                                                
aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCAc                                                                                                                                                >  2:1138506/1‑151 (MQ=255)
aGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCAc                                                                                                                                                <  2:1475066/151‑1 (MQ=255)
 gCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACt                                                                                                                                               <  1:1109149/151‑1 (MQ=255)
  ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTg                                                                                                                                              <  2:190324/151‑1 (MQ=255)
  ccGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTg                                                                                                                                              <  1:1421950/151‑1 (MQ=255)
   cGTAGAAAGAAGAACCGGTTATAACGCTTTGCGCCGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTgg                                                                                                                                             <  1:1260847/151‑1 (MQ=255)
     tAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGtt                                                                                                                                           >  2:1881185/1‑151 (MQ=255)
       gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAc                                                                                                                                         <  1:591665/151‑1 (MQ=255)
       gAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAc                                                                                                                                         >  2:1337846/1‑151 (MQ=255)
        aaGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcg                                                                                                                                        <  1:5387/151‑1 (MQ=255)
         aGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTAcgc                                                                                                                                       >  2:441049/1‑151 (MQ=255)
              gAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCt                                                                                                                                  <  1:410428/151‑1 (MQ=255)
              gAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCt                                                                                                                                  <  2:688120/151‑1 (MQ=255)
               aaCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCtt                                                                                                                                 >  2:21200/1‑151 (MQ=255)
                aCGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTc                                                                                                                                <  2:781964/151‑1 (MQ=255)
                 cgcgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTcc                                                                                                                               >  2:213153/1‑151 (MQ=255)
                   cgTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCg                                                                                                                              >  2:565238/1‑150 (MQ=255)
                        tatCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATcac                                                                                                                         >  1:1486605/1‑150 (MQ=255)
                              tttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGc                                                                                                                  >  1:1654818/1‑151 (MQ=255)
                               ttGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGc                                                                                                                  <  2:960604/150‑1 (MQ=255)
                                  cgcgGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACt                                                                                                              <  2:1256908/151‑1 (MQ=255)
                                     gggAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGt                                                                                                           >  2:1698790/1‑151 (MQ=255)
                                       gAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTg                                                                                                         <  2:1074749/151‑1 (MQ=255)
                                         aaTGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGa                                                                                                        >  2:814889/1‑150 (MQ=255)
                                            gTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                                                                                                    <  2:1492118/151‑1 (MQ=255)
                                             tCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTc                                                                                                    >  2:1982472/1‑150 (MQ=255)
                                               gCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                                                                                 >  1:1418382/1‑151 (MQ=255)
                                               gCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                                                                                 >  1:731834/1‑151 (MQ=255)
                                                cTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGt                                                                                                 <  1:1174939/150‑1 (MQ=255)
                                                    gcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg                                                                                            >  2:1884277/1‑151 (MQ=255)
                                                    gcCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCgg                                                                                            >  2:48240/1‑151 (MQ=255)
                                                     ccAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                                                                           <  1:1036187/151‑1 (MQ=255)
                                                      cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                                                                           >  2:995266/1‑150 (MQ=255)
                                                      cAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGc                                                                                           >  2:1369141/1‑150 (MQ=255)
                                                       aGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCa                                                                                          >  1:1359906/1‑150 (MQ=255)
                                                       aGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGAGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCa                                                                                          >  1:1190055/1‑150 (MQ=255)
                                                         gTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGc                                                                                       <  1:2060108/151‑1 (MQ=255)
                                                          tGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGcc                                                                                      >  2:1918795/1‑151 (MQ=255)
                                                            cGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTg                                                                                    >  1:696715/1‑151 (MQ=255)
                                                              ttttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat                                                                                  >  1:303723/1‑151 (MQ=255)
                                                               tttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGat                                                                                  <  2:624877/150‑1 (MQ=255)
                                                                ttACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGata                                                                                 <  1:577169/150‑1 (MQ=255)
                                                                 tACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataa                                                                                <  2:891584/150‑1 (MQ=255)
                                                                    cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                                                                            <  2:658328/151‑1 (MQ=255)
                                                                    cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaataa                                                                            <  2:343901/151‑1 (MQ=255)
                                                                    cGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaata                                                                             >  1:1696398/1‑150 (MQ=255)
                                                                      atgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                                                                          >  1:429523/1‑151 (MQ=255)
                                                                      atgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                                                                          >  2:1829586/1‑151 (MQ=255)
                                                                      atgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgg                                                                          >  2:473559/1‑151 (MQ=255)
                                                                       tgatCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGataaata                                                                             <  2:1342071/147‑1 (MQ=255)
                                                                           cAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGt                                                                     >  2:1272551/1‑151 (MQ=255)
                                                                           cAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGt                                                                     <  2:745909/151‑1 (MQ=255)
                                                                               gcagTCGGGAGTTTGGTCGCCCGCTCGATTTTGTTTTATAAAGTGGTATGAAAGTAAGTGAAGCTTATGAAATGGTTACGCGCGCTTCAGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAgggagggcagt                                                                 <  2:1515525/148‑10 (MQ=255)
                                                                               gcCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAgg                                                                 >  1:749849/1‑151 (MQ=255)
                                                                                 cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                                                               <  1:612335/151‑1 (MQ=255)
                                                                                 cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                                                               >  2:1060743/1‑151 (MQ=255)
                                                                                 cGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTc                                                               >  1:1463172/1‑151 (MQ=255)
                                                                                  ggcGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCt                                                              <  2:1989588/149‑1 (MQ=255)
                                                                                   tCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTg                                                             >  2:366595/1‑151 (MQ=255)
                                                                                    cGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTg                                                             <  1:1480332/150‑1 (MQ=255)
                                                                                    cGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTg                                                             <  1:1122341/150‑1 (MQ=255)
                                                                                     gggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGcc                                                           >  1:1701050/1‑151 (MQ=255)
                                                                                      ggCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCca                                                          <  2:2153376/151‑1 (MQ=255)
                                                                                       gCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCcac                                                         >  1:783892/1‑151 (MQ=255)
                                                                                                cGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAc                                                <  1:2104020/151‑1 (MQ=255)
                                                                                                cGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAc                                                >  1:817311/1‑151 (MQ=255)
                                                                                                 gCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAcc                                               >  1:1772952/1‑151 (MQ=255)
                                                                                                  cccGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGAcc                                               >  1:1190852/1‑150 (MQ=255)
                                                                                                    cGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAAc                                            <  2:1855630/151‑1 (MQ=255)
                                                                                                     gCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACg                                           >  1:104120/1‑151 (MQ=255)
                                                                                                     gCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACg                                           >  1:665593/1‑151 (MQ=255)
                                                                                                        cGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGttt                                        <  1:1140084/151‑1 (MQ=255)
                                                                                                           ttttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGgc                                     >  1:167297/1‑151 (MQ=255)
                                                                                                           ttttGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGgc                                     >  1:2076632/1‑151 (MQ=255)
                                                                                                           ttttGTTTTCTACCGTGGACTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGTTCTGCCACAGGGCAGACCAACGTTTGgc                                     >  1:149707/1‑151 (MQ=255)
                                                                                                               gTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTg                                 >  1:1793354/1‑151 (MQ=255)
                                                                                                                 tttCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTgcg                               >  1:1996740/1‑151 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaaa                          <  2:223566/151‑1 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaaa                          <  2:1595151/151‑1 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaa                           >  1:2114930/1‑150 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaa                           >  2:696627/1‑150 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaaa                           >  2:1928021/1‑150 (MQ=255)
                                                                                                                      aCCGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCaa                            <  2:1734714/149‑1 (MQ=255)
                                                                                                                       ccGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAAc                         >  1:14735/1‑151 (MQ=255)
                                                                                                                       ccGTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAAc                         <  1:1837223/151‑1 (MQ=255)
                                                                                                                         gTGGTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGt                       >  2:314611/1‑151 (MQ=255)
                                                                                                                           ggTCTGAACGTCAGTGAAGCTTCTGCGCTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGa                     <  2:289737/151‑1 (MQ=255)
                                                                                                                           ggTCTGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTg                      >  1:998408/1‑150 (MQ=255)
                                                                                                                               tGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAgcgc                 >  1:2092797/1‑151 (MQ=255)
                                                                                                                               tGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAgcgc                 >  1:1379677/1‑151 (MQ=255)
                                                                                                                               tGAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAgcgc                 >  2:1275889/1‑151 (MQ=255)
                                                                                                                                gAACGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAgcgc                 <  2:80460/150‑1 (MQ=255)
                                                                                                                                  aCGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCggt              >  1:2049018/1‑151 (MQ=255)
                                                                                                                                   cGTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCggtg             >  2:390489/1‑151 (MQ=255)
                                                                                                                                    gTCAGTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCggtgg            >  1:1390590/1‑151 (MQ=255)
                                                                                                                                        gTGAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGc        >  2:335542/1‑151 (MQ=255)
                                                                                                                                          gAAGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCg       <  2:455511/150‑1 (MQ=255)
                                                                                                                                           aaGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTa     <  2:1493985/151‑1 (MQ=255)
                                                                                                                                            aGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTAt    <  2:635786/151‑1 (MQ=255)
                                                                                                                                            aGCTTCTGCACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTAt    >  2:1549564/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAGCGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGTGAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGTCCCGGCAAGCCTGATAAATAAGGTATGTCAGGTCTGCCACAGGGCAGACCAACGTTTGGCGCTGCGCAAAACGTGAGCGCGGTGGTGGCGTATGA  >  NC_000913/231704‑231996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑