Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,280,131 A→G E406E (GAA→GAG)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,280,1310AG100.0% 501.1 / NA 132E406E (GAA→GAG) kbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (73/59);  total (73/59)

Only 100 of 132 total aligned reads displayed.

TGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCA  >  NC_000913/3279985‑3280278
                                                                                                                                                  |                                                                                                                                                   
tGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTc                                                                                                                                                  >  2:34260/1‑150 (MQ=255)
  gCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCAtt                                                                                                                                               >  2:2026739/1‑151 (MQ=255)
   ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTa                                                                                                                                              <  2:1392299/151‑1 (MQ=255)
   ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTa                                                                                                                                              <  1:1823842/151‑1 (MQ=255)
      gACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATc                                                                                                                                            >  2:191126/1‑150 (MQ=255)
        cAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAAc                                                                                                                                         <  2:2042302/151‑1 (MQ=255)
         aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAAcc                                                                                                                                        >  1:845784/1‑151 (MQ=255)
         aGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAAcc                                                                                                                                        <  1:1815075/151‑1 (MQ=255)
            cAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCat                                                                                                                                      >  1:450166/1‑150 (MQ=255)
               aTTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTc                                                                                                                                  >  1:1610630/1‑151 (MQ=255)
                ttGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTc                                                                                                                                  >  2:127262/1‑150 (MQ=255)
                  gatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAgg                                                                                                                               >  2:1461667/1‑151 (MQ=255)
                     gaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGAc                                                                                                                             <  2:1271530/150‑1 (MQ=255)
                      aCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACAt                                                                                                                           <  1:2167582/151‑1 (MQ=255)
                      aCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACAt                                                                                                                           <  2:261920/151‑1 (MQ=255)
                        gCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATcc                                                                                                                         >  2:142374/1‑151 (MQ=255)
                             cGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcg                                                                                                                    >  2:943665/1‑151 (MQ=255)
                             cGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgc                                                                                                                     <  2:1277194/150‑1 (MQ=255)
                              gCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcgc                                                                                                                   >  1:139929/1‑151 (MQ=255)
                              gCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcgc                                                                                                                   >  2:2026628/1‑151 (MQ=255)
                                tCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGgcgc                                                                                                                   >  1:1175381/1‑149 (MQ=255)
                                      ggTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTa                                                                                                               <  1:902581/147‑1 (MQ=255)
                                       gTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcac                                                                                                           >  2:542549/1‑150 (MQ=255)
                                         aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacaca                                                                                                        <  1:1644987/151‑1 (MQ=255)
                                         aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacac                                                                                                         >  2:214848/1‑150 (MQ=255)
                                             aaTCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCt                                                                                                    >  1:1654322/1‑151 (MQ=255)
                                             aaTCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCt                                                                                                    <  1:291074/151‑1 (MQ=255)
                                                 tGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgtg                                                                                                 >  2:1651481/1‑150 (MQ=255)
                                                 tGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgtg                                                                                                 >  2:503375/1‑150 (MQ=255)
                                                  ggCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGa                                                                                                >  2:1553742/1‑150 (MQ=255)
                                                   gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGa                                                                                                >  2:1944600/1‑149 (MQ=255)
                                                   gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAg                                                                                              <  2:1294371/151‑1 (MQ=255)
                                                   gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAg                                                                                              <  1:127262/151‑1 (MQ=255)
                                                     ggATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGc                                                                                            >  1:1029728/1‑151 (MQ=255)
                                                      gATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGcc                                                                                           <  2:523050/151‑1 (MQ=255)
                                                      gATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGcc                                                                                           <  1:1019655/151‑1 (MQ=255)
                                                         tCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAAt                                                                                        >  1:1832541/1‑151 (MQ=255)
                                                           aCCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                       >  1:2168664/1‑150 (MQ=255)
                                                            ccAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATaa                                                                                      <  2:2136783/150‑1 (MQ=255)
                                                            ccAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATa                                                                                       >  1:624887/1‑149 (MQ=255)
                                                            ccAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAg                                                                                     <  2:1443446/151‑1 (MQ=255)
                                                             cAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGc                                                                                    <  1:274973/151‑1 (MQ=255)
                                                             cAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGc                                                                                    <  1:1970236/151‑1 (MQ=255)
                                                                 tCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaaa                                                                                 >  2:588106/1‑150 (MQ=255)
                                                                  ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAAc                                                                               <  2:628988/151‑1 (MQ=255)
                                                                   cgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaaaa                                                                                <  2:1638246/149‑1 (MQ=255)
                                                                     ctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACaa                                                                             >  1:2073429/1‑150 (MQ=255)
                                                                      tgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACaa                                                                             >  1:1052700/1‑149 (MQ=255)
                                                                       gccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACaaa                                                                            <  2:1164619/149‑1 (MQ=255)
                                                                       gccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACaaa                                                                            <  2:1912553/149‑1 (MQ=255)
                                                                          gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                        >  2:623102/1‑150 (MQ=255)
                                                                            tgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAac                                                                     >  1:1613785/1‑151 (MQ=255)
                                                                              aTCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAacac                                                                   >  1:1808333/1‑151 (MQ=255)
                                                                                cAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACg                                                                  <  1:1353565/150‑1 (MQ=255)
                                                                                 aGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCt                                                                <  1:210757/151‑1 (MQ=255)
                                                                                  gCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCt                                                                >  2:1323601/1‑150 (MQ=255)
                                                                                  gCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTa                                                               <  1:1976989/151‑1 (MQ=255)
                                                                                    cAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATg                                                             >  1:870063/1‑151 (MQ=255)
                                                                                       tATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGcc                                                           >  2:1519790/1‑150 (MQ=255)
                                                                                          cTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                       >  1:1395847/1‑151 (MQ=255)
                                                                                          cTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                       >  2:103884/1‑151 (MQ=255)
                                                                                          cTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAat                                                       >  2:452309/1‑151 (MQ=255)
                                                                                            gCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCaa                                                         <  1:2139422/147‑1 (MQ=255)
                                                                                             ccGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATAtt                                                    >  1:568540/1‑151 (MQ=255)
                                                                                               gCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGt                                                  >  2:1706452/1‑151 (MQ=255)
                                                                                                  gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                                <  2:2033175/150‑1 (MQ=255)
                                                                                                  gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                                <  1:1016976/150‑1 (MQ=255)
                                                                                                  gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                                <  1:425057/150‑1 (MQ=255)
                                                                                                  gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                                <  2:1512618/150‑1 (MQ=255)
                                                                                                   cAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtttt                                               >  1:2157381/1‑150 (MQ=255)
                                                                                                    aGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTaa                                             <  2:650475/151‑1 (MQ=255)
                                                                                                    aGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTaa                                             <  2:447944/151‑1 (MQ=255)
                                                                                                       aCGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGcc                                          <  1:1979369/151‑1 (MQ=255)
                                                                                                       aCGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGc                                           <  1:1988247/150‑1 (MQ=255)
                                                                                                         gTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCgg                                        >  1:1329968/1‑151 (MQ=255)
                                                                                                          tGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCgg                                        >  2:590343/1‑150 (MQ=255)
                                                                                                           gAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAt                                      >  2:835949/1‑151 (MQ=255)
                                                                                                            aaaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAtt                                     >  2:1499330/1‑151 (MQ=255)
                                                                                                            aaaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAtt                                     <  1:1086689/151‑1 (MQ=255)
                                                                                                            aaaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAtt                                     <  1:1209623/151‑1 (MQ=255)
                                                                                                             aaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtg                                    <  2:769943/151‑1 (MQ=255)
                                                                                                              aGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                   <  2:1079428/151‑1 (MQ=255)
                                                                                                               gTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgat                                  >  1:1275414/1‑151 (MQ=255)
                                                                                                                ttCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgatg                                 >  2:594184/1‑151 (MQ=255)
                                                                                                                  cGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtgatga                                <  2:325684/150‑1 (MQ=255)
                                                                                                                    cTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACg                             >  2:451064/1‑151 (MQ=255)
                                                                                                                      ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACg                             <  1:993808/149‑1 (MQ=255)
                                                                                                                      ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCt                           >  1:2118666/1‑151 (MQ=255)
                                                                                                                        ggCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTg                         <  2:279451/151‑1 (MQ=255)
                                                                                                                          cGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAt                       <  2:1610602/151‑1 (MQ=255)
                                                                                                                           gAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                      >  1:556821/1‑151 (MQ=255)
                                                                                                                           gAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                      >  1:2021340/1‑151 (MQ=255)
                                                                                                                            aGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTc                     <  2:1618152/151‑1 (MQ=255)
                                                                                                                               tGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACg                  >  1:1104274/1‑151 (MQ=255)
                                                                                                                                gCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACg                  <  1:1687927/150‑1 (MQ=255)
                                                                                                                                   gCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTc               >  1:1383515/1‑150 (MQ=255)
                                                                                                                                            aCGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGt     >  1:297224/1‑151 (MQ=255)
                                                                                                                                             cGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGtt    <  1:1323624/151‑1 (MQ=255)
                                                                                                                                              gggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc   <  1:161942/151‑1 (MQ=255)
                                                                                                                                               ggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCa  <  2:1023043/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTCA  >  NC_000913/3279985‑3280278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑