BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,373,078	T→C	T167A (ACC→GCC)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,373,078	0	T	C	100.0%	455.6 / NA	119	T167A (ACC→GCC)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/66); total (53/66)

Only 100 of 119 total aligned reads displayed.

GCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA  >  NC_000913/3372931‑3373222

gcCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTg                                                                                                                                               >  2:442148/1‑151 (MQ=255)

 ccAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGt                                                                                                                                              <  1:964276/151‑1 (MQ=255)

   aGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGttt                                                                                                                                            >  1:332893/1‑151 (MQ=255)

     cccATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGttt                                                                                                                                            <  2:553137/149‑1 (MQ=255)

      ccATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAg                                                                                                                                         <  1:1438727/151‑1 (MQ=255)

        atgatgTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAgc                                                                                                                                        <  1:1444933/150‑1 (MQ=255)

           ctgttgtAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCAc                                                                                                                                    >  2:2060593/2‑151 (MQ=255)

           atgTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCAc                                                                                                                                    >  1:1812515/1‑151 (MQ=255)

             gttgtAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCt                                                                                                                                  <  1:1727167/151‑1 (MQ=255)

              ttgtAGGTACTGCCGATACCCCCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCt                                                                                                                                  <  2:1243986/150‑1 (MQ=255)

              ttgtAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCt                                                                                                                                  <  1:1420928/150‑1 (MQ=255)

               tgtAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTAc                                                                                                                                >  2:438915/1‑151 (MQ=255)

                gtgggcaCTGCCGTTCCCACCAGCAGCGACCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTAc                                                                                                                                <  2:1022883/144‑1 (MQ=255)

                   ggTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCa                                                                                                                            <  1:1129106/151‑1 (MQ=255)

                     tACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAgg                                                                                                                          >  2:78378/1‑151 (MQ=255)

                       cTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGc                                                                                                                         <  2:1193501/150‑1 (MQ=255)

                        tGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCa                                                                                                                        >  1:670447/1‑150 (MQ=255)

                         gCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCa                                                                                                                        <  2:337370/149‑1 (MQ=255)

                         gCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCa                                                                                                                        <  2:75328/149‑1 (MQ=255)

                          ccGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATg                                                                                                                     <  2:1537666/151‑1 (MQ=255)

                             aTACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTaa                                                                                                                  <  1:667769/151‑1 (MQ=255)

                               accaccATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAAc                                                                                                                 <  1:119692/150‑1 (MQ=255)

                               accaccATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAAc                                                                                                                 >  1:676908/1‑150 (MQ=255)

                                 caccaTCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAg                                                                                                              <  2:825000/151‑1 (MQ=255)

                                   ccaTCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAgtg                                                                                                            <  1:933728/151‑1 (MQ=255)

                                   ccaTCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAgt                                                                                                             <  1:307965/150‑1 (MQ=255)

                                       cAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGtt                                                                                                        <  2:1556055/151‑1 (MQ=255)

                                       cAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGtt                                                                                                        >  1:1114998/1‑151 (MQ=255)

                                        aGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtg                                                                                                       >  2:357592/1‑151 (MQ=255)

                                            cccgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatct                                                                                                   >  1:1663803/1‑151 (MQ=255)

                                              cgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctg                                                                                                  >  2:1490594/1‑150 (MQ=255)

                                              cgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctg                                                                                                  >  2:1462521/1‑150 (MQ=255)

                                               gccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctgat                                                                                                <  1:682707/151‑1 (MQ=255)

                                                ccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGATCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctgatc                                                                                               <  2:651217/151‑1 (MQ=255)

                                                 cagcagACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATcc                                                                                              <  2:1508824/151‑1 (MQ=255)

                                                 cagcagACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATcc                                                                                              <  1:659966/151‑1 (MQ=255)

                                                  agcagACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATcc                                                                                              >  2:233416/1‑150 (MQ=255)

                                                    cagACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAgg                                                                                           >  2:133587/1‑151 (MQ=255)

                                                     agACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAggg                                                                                          <  1:69660/151‑1 (MQ=255)

                                                       aCCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTc                                                                                        <  2:395632/151‑1 (MQ=255)

                                                        ccAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTc                                                                                        >  1:1322953/1‑150 (MQ=255)

                                                          agagGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGt                                                                                     >  1:1963883/1‑151 (MQ=255)

                                                           gagGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGtt                                                                                    <  1:104083/151‑1 (MQ=255)

                                                                gAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTAcc                                                                               <  1:162510/151‑1 (MQ=255)

                                                                 aaGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTAccc                                                                              <  2:16097/151‑1 (MQ=255)

                                                                    aTTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCAc                                                                           <  1:952574/151‑1 (MQ=255)

                                                                       tcgtcgTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTc                                                                         <  2:852721/150‑1 (MQ=255)

                                                                        cgtcgtAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTc                                                                         >  1:929244/1‑149 (MQ=255)

                                                                            gtAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCt                                                                   >  1:2029937/1‑151 (MQ=255)

                                                                               aCCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGa                                                                <  1:1941666/151‑1 (MQ=255)

                                                                                 cGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAt                                                              <  2:1848135/151‑1 (MQ=255)

                                                                                 cGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAt                                                              >  2:2086102/1‑151 (MQ=255)

                                                                                  gTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAtg                                                             >  1:1227164/1‑151 (MQ=255)

                                                                                      taGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAtgttgt                                                         >  1:772900/1‑151 (MQ=255)

                                                                                      taGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAtgttgt                                                         >  1:1277085/1‑151 (MQ=255)

                                                                                       agagCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCGGTTTTACCCCACTCAGGGCTGGAAtgttg                                                          >  1:546419/1‑149 (MQ=255)

                                                                                          gCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTacac                                                     <  1:1324497/151‑1 (MQ=255)

                                                                                           cacaAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTAcacc                                                    >  2:1448862/1‑151 (MQ=255)

                                                                                             caAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTAcaccac                                                  >  2:1177625/1‑151 (MQ=255)

                                                                                               aGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACaccacc                                                 >  1:1341974/1‑150 (MQ=255)

                                                                                                 aTCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                              >  2:1096344/1‑151 (MQ=255)

                                                                                                   cAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCgg                                            <  1:760822/151‑1 (MQ=255)

                                                                                                    aGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGc                                           <  2:160617/151‑1 (MQ=255)

                                                                                                      gATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCa                                          >  2:19061/1‑150 (MQ=255)

                                                                                                       aTGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaaa                                        <  2:249276/151‑1 (MQ=255)

                                                                                                         gTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAc                                       <  2:1333768/150‑1 (MQ=255)

                                                                                                          ttCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAcc                                      >  1:2015230/1‑150 (MQ=255)

                                                                                                            cACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCAt                                    <  2:525060/150‑1 (MQ=255)

                                                                                                              cgacgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATcc                                  >  2:1868746/1‑150 (MQ=255)

                                                                                                               gacgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgc                                <  1:1107072/151‑1 (MQ=255)

                                                                                                                acgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgcc                               >  2:406261/1‑151 (MQ=255)

                                                                                                                acgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgcc                               >  2:112454/1‑151 (MQ=255)

                                                                                                                acgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgcc                               <  2:1762926/151‑1 (MQ=255)

                                                                                                                 cgGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgccg                              <  1:703810/151‑1 (MQ=255)

                                                                                                                      cTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                          >  2:1337086/1‑150 (MQ=255)

                                                                                                                       tGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCa                         >  1:567204/1‑150 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                            <  1:653372/146‑1 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                            <  1:2108343/146‑1 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAAt                           <  2:176271/147‑1 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAAt                           <  1:2047396/147‑1 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCa                         <  1:684629/149‑1 (MQ=255)

                                                                                                                        gCTCCATCTGATAGAGAGCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCCCCATCGGCAAACCATCCGCCGac                            <  2:1982754/146‑2 (MQ=255)

                                                                                                                           ccATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                    >  1:304798/1‑151 (MQ=255)

                                                                                                                           ccATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                    <  1:357265/151‑1 (MQ=255)

                                                                                                                           ccATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataa                     <  1:836580/150‑1 (MQ=255)

                                                                                                                            cATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                   >  2:1313837/1‑151 (MQ=255)

                                                                                                                            cATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                   <  2:1600902/151‑1 (MQ=255)

                                                                                                                             aTCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTg                  >  1:2134512/1‑151 (MQ=255)

                                                                                                                                tGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGccc               >  1:78546/1‑151 (MQ=255)

                                                                                                                                 gATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg              <  1:1026832/151‑1 (MQ=255)

                                                                                                                                 gATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg              <  1:1998948/151‑1 (MQ=255)

                                                                                                                                  aTAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg              >  2:894458/1‑150 (MQ=255)

                                                                                                                                     gagaTCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGAt            >  1:495425/1‑149 (MQ=255)

                                                                                                                                      agaTCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGt         >  2:2069876/1‑151 (MQ=255)

                                                                                                                                       gaTCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg        <  1:149238/151‑1 (MQ=255)

                                                                                                                                          cGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAt      <  2:1686662/150‑1 (MQ=255)

                                                                                                                                           gCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCg    >  1:1473731/1‑151 (MQ=255)

                                                                                                                                           gCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCg    <  2:2059340/151‑1 (MQ=255)

                                                                                                                                            ccAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc   <  2:1323890/151‑1 (MQ=255)

                                                                                                                                             cAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa  <  1:1872906/151‑1 (MQ=255)

GCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA  >  NC_000913/3372931‑3373222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`