Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,575,211 T→C A47A (GCT→GCC)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,575,2110TC98.6% 535.5 / ‑2.5 145A47A (GCT→GCC) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (62/80);  total (64/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 149 total aligned reads displayed.

TTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAG  >  NC_000913/3575064‑3575357
                                                                                                                                                   |                                                                                                                                                  
ttGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGccct                                                                                                                                                  <  1:1870258/150‑1 (MQ=255)
  gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCcctcct                                                                                                                                               >  1:2143510/1‑151 (MQ=255)
  gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCcctcct                                                                                                                                               >  2:625701/1‑151 (MQ=255)
      tATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTggcg                                                                                                                                           >  2:2000871/1‑149 (MQ=255)
      tATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGt                                                                                                                                            <  2:1621413/150‑1 (MQ=255)
       atgaatgaAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTg                                                                                                                                           <  1:971667/150‑1 (MQ=255)
         gaatgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGAt                                                                                                                                         <  1:1281546/150‑1 (MQ=255)
           atgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCt                                                                                                                                      <  2:1511420/151‑1 (MQ=255)
           atgaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCt                                                                                                                                      <  2:1757132/151‑1 (MQ=255)
             gaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTg                                                                                                                                     <  1:1996279/150‑1 (MQ=255)
              aaaTCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGgt                                                                                                                                   >  2:1863356/1‑151 (MQ=255)
                    tttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCt                                                                                                                             >  2:981147/1‑151 (MQ=255)
                     ttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCt                                                                                                                             <  2:2088540/150‑1 (MQ=255)
                         gcagcaGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGttt                                                                                                                         <  1:1552142/150‑1 (MQ=255)
                         gcagcaGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGttt                                                                                                                         <  2:765276/150‑1 (MQ=255)
                         gcagcaGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGtt                                                                                                                          <  1:1182752/149‑1 (MQ=255)
                         gcagcaGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTg                                                                                                                        >  2:1248309/1‑151 (MQ=255)
                         gcagcaGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTg                                                                                                                        <  1:296696/151‑1 (MQ=255)
                          cagcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGc                                                                                                                       <  1:783457/151‑1 (MQ=255)
                            gcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCgg                                                                                                                     <  2:441392/151‑1 (MQ=255)
                              agTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGt                                                                                                                   >  1:1466695/1‑151 (MQ=255)
                               gTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                                                                                  >  1:2123281/1‑151 (MQ=255)
                                ttttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                                                                                  >  2:1655720/1‑150 (MQ=255)
                                 tttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTga                                                                                                                 <  2:1351666/150‑1 (MQ=255)
                                  ttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                                                                                  >  2:1461962/1‑148 (MQ=255)
                                    aTTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaa                                                                                                              <  2:1503102/150‑1 (MQ=255)
                                    aTTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaa                                                                                                              >  2:854325/1‑150 (MQ=255)
                                    aTTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaa                                                                                                              >  2:532212/1‑150 (MQ=255)
                                      tGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAAt                                                                                                           >  2:639800/1‑151 (MQ=255)
                                       gATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAAt                                                                                                           <  1:1406759/150‑1 (MQ=255)
                                        aTCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTc                                                                                                         >  2:1770488/1‑151 (MQ=255)
                                          ccTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAAGCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                                                                                  <  1:1849463/140‑1 (MQ=255)
                                            tGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGc                                                                                                     >  1:1306269/1‑151 (MQ=255)
                                             gATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGc                                                                                                     <  1:1424661/150‑1 (MQ=255)
                                             gATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCa                                                                                                    <  2:75660/151‑1 (MQ=255)
                                              aTTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCAt                                                                                                   <  2:762884/151‑1 (MQ=255)
                                                  tGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCt                                                                                               >  2:1288023/1‑151 (MQ=255)
                                                    gATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTa                                                                                             <  1:826527/151‑1 (MQ=255)
                                                     aTCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCtt                                                                                              >  2:1456035/1‑149 (MQ=255)
                                                       ccGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGcc                                                                                          >  1:2049987/1‑151 (MQ=255)
                                                       ccGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGcc                                                                                          >  2:821834/1‑151 (MQ=255)
                                                         gCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCt                                                                                         >  2:1650452/1‑150 (MQ=255)
                                                            cGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGa                                                                                     <  2:1612804/151‑1 (MQ=255)
                                                            cGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGa                                                                                     <  1:442818/151‑1 (MQ=255)
                                                             ggAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACg                                                                                      >  1:728109/1‑149 (MQ=255)
                                                              gAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGc                                                                                   <  1:930502/151‑1 (MQ=255)
                                                                aaCCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGc                                                                                   >  1:1003784/1‑149 (MQ=255)
                                                                 acctacctATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGa                                                                                <  1:784170/151‑1 (MQ=255)
                                                                  cctacctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGaa                                                                               <  1:540375/151‑1 (MQ=255)
                                                                  cctacctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGaa                                                                               <  1:809094/151‑1 (MQ=255)
                                                                     acctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAAcc                                                                            <  1:1803755/151‑1 (MQ=255)
                                                                      cctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCg                                                                           <  2:1883957/151‑1 (MQ=255)
                                                                      cctaTTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCg                                                                           <  2:72127/151‑1 (MQ=255)
                                                                            ttCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTcc                                                                      <  2:466034/150‑1 (MQ=255)
                                                                            ttCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTcc                                                                      <  1:715998/150‑1 (MQ=255)
                                                                              cATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAtt                                                                   >  1:308368/1‑151 (MQ=255)
                                                                              cATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAtt                                                                   <  2:1843390/151‑1 (MQ=255)
                                                                              cATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAt                                                                    <  2:541988/150‑1 (MQ=255)
                                                                               aTGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAttt                                                                  <  1:623397/151‑1 (MQ=255)
                                                                                tGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAttt                                                                  <  1:214082/150‑1 (MQ=255)
                                                                                 gTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCt                                                                <  1:1964672/151‑1 (MQ=255)
                                                                                  tCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTg                                                               >  1:1074114/1‑151 (MQ=255)
                                                                                    cGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTggc                                                             <  1:1442912/151‑1 (MQ=255)
                                                                                       aCTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTggcggc                                                          <  2:427862/151‑1 (MQ=255)
                                                                                         tGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcat                                                        >  1:1004778/1‑151 (MQ=255)
                                                                                          gAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatc                                                       <  2:397817/151‑1 (MQ=255)
                                                                                           aaaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatca                                                      <  1:466145/151‑1 (MQ=255)
                                                                                            aaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatcat                                                     <  1:1834029/151‑1 (MQ=255)
                                                                                            aaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatcat                                                     >  2:2100151/1‑151 (MQ=255)
                                                                                            aaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatcat                                                     <  1:1043821/151‑1 (MQ=255)
                                                                                            aaCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGcatcat                                                     >  1:943107/1‑151 (MQ=255)
                                                                                             aCATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCAtt                                                    >  2:1334325/1‑151 (MQ=255)
                                                                                              cATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTc                                                   >  1:1166945/1‑151 (MQ=255)
                                                                                                tACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTg                                                 >  1:1247550/1‑151 (MQ=255)
                                                                                                 aCTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGt                                                <  1:1786170/151‑1 (MQ=255)
                                                                                                   tGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGttt                                              >  1:1131400/1‑151 (MQ=255)
                                                                                                    gAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTc                                             >  2:1845762/1‑151 (MQ=255)
                                                                                                       ccGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTg                                           >  2:785928/1‑150 (MQ=255)
                                                                                                           aaaGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGc                                      <  2:2134505/151‑1 (MQ=255)
                                                                                                            aaGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGcc                                     >  2:262257/1‑151 (MQ=255)
                                                                                                                cggcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCAtt                                  >  2:1033917/1‑150 (MQ=255)
                                                                                                                cggcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTa                                 <  2:1140751/151‑1 (MQ=255)
                                                                                                                 ggcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTaa                                <  2:213662/151‑1 (MQ=255)
                                                                                                                 ggcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTaa                                <  1:2026660/151‑1 (MQ=255)
                                                                                                                  gcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTaaa                               <  1:1873829/151‑1 (MQ=255)
                                                                                                                  gcggGCAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTaa                                >  2:659410/1‑150 (MQ=255)
                                                                                                                       cAATCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGAt                          <  2:707444/151‑1 (MQ=255)
                                                                                                                        aaTCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGAtt                         >  1:1003565/1‑151 (MQ=255)
                                                                                                                          tCATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGAtttt                       >  2:2008679/1‑151 (MQ=255)
                                                                                                                           cATGGTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTc                      <  2:106491/151‑1 (MQ=255)
                                                                                                                               gTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCa                  <  2:726316/151‑1 (MQ=255)
                                                                                                                               gTGCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCa                  <  1:945686/151‑1 (MQ=255)
                                                                                                                                 gCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAg                 <  2:461942/150‑1 (MQ=255)
                                                                                                                                 gCGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAg                 <  2:973003/150‑1 (MQ=255)
                                                                                                                                  cGAGAGTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAgcg               >  2:1213376/1‑151 (MQ=255)
                                                                                                                                      agTTGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTc           >  1:1227272/1‑151 (MQ=255)
                                                                                                                                         tGCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAgg        >  1:2143021/1‑151 (MQ=255)
                                                                                                                                          gCTTATTGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGa       <  1:1639499/151‑1 (MQ=255)
                                                                                                                                               ttGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAAt    >  1:1044282/1‑149 (MQ=255)
                                                                                                                                                tGCCCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATag  <  1:90714/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCATTTCTGGCGGCATCATTCTGTTTCTGATCGCCATTAAAATGATTTTCCCCAGCGCTTCAGGAAATAG  >  NC_000913/3575064‑3575357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑