Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 268,643 C→T A106A (GCG→GCA ykfA ← CP4‑6 prophage; putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913268,6430CT99.3% 541.5 / NA 136A106A (GCG→GCAykfACP4‑6 prophage; putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (71/64);  minor base G (0/1);  total (71/65)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 137 total aligned reads displayed.

CCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGG  >  NC_000913/268495‑268788
                                                                                                                                                    |                                                                                                                                                 
ccGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAgtgc                                                                                                                                                 >  2:142845/1‑151 (MQ=255)
 cGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTgca                                                                                                                                                <  2:140436/151‑1 (MQ=255)
    cTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTgcagc                                                                                                                                              <  2:604749/150‑1 (MQ=255)
       gCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATAc                                                                                                                                          <  2:1919942/151‑1 (MQ=255)
         tGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTc                                                                                                                                        >  2:1003578/1‑151 (MQ=255)
         tGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTc                                                                                                                                        >  1:448312/1‑151 (MQ=255)
          gATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACt                                                                                                                                         <  1:399334/149‑1 (MQ=255)
          gATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCg                                                                                                                                       <  2:1947127/151‑1 (MQ=255)
              acaaacaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTAt                                                                                                                                   <  1:606618/151‑1 (MQ=255)
               caaacaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATc                                                                                                                                  >  1:1212034/1‑151 (MQ=255)
                aaacaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGTCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATcg                                                                                                                                 <  2:1283416/151‑1 (MQ=255)
                aaacaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATcg                                                                                                                                 >  1:38666/1‑151 (MQ=255)
                aaacaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATcg                                                                                                                                 >  1:1115985/1‑151 (MQ=255)
                  acaGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATcgcg                                                                                                                               >  2:925330/1‑151 (MQ=255)
                      cACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAgcg                                                                                                                           <  2:1182285/151‑1 (MQ=255)
                      cACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAgcg                                                                                                                           <  1:1143698/151‑1 (MQ=255)
                           tATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCa                                                                                                                        <  2:473619/149‑1 (MQ=255)
                           tATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCa                                                                                                                        <  2:1426482/149‑1 (MQ=255)
                            aTGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACtt                                                                                                                     <  2:669857/151‑1 (MQ=255)
                            aTGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACt                                                                                                                      <  2:162238/150‑1 (MQ=255)
                            aTGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACt                                                                                                                      <  1:1163392/150‑1 (MQ=255)
                            aTGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACt                                                                                                                      <  2:1516433/150‑1 (MQ=255)
                              gCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACttt                                                                                                                    <  2:2074253/150‑1 (MQ=255)
                              gCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTc                                                                                                                   <  2:705050/151‑1 (MQ=255)
                              gCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTc                                                                                                                   >  1:570697/1‑151 (MQ=255)
                               ccGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCg                                                                                                                  >  1:835510/1‑151 (MQ=255)
                                 ggTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGcc                                                                                                                >  1:295776/1‑151 (MQ=255)
                                    ataCCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCa                                                                                                              <  2:1659419/150‑1 (MQ=255)
                                      aCCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGc                                                                                                           >  2:1353414/1‑151 (MQ=255)
                                       ccTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGcc                                                                                                          <  1:215030/151‑1 (MQ=255)
                                         tctcCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCgg                                                                                                        >  1:1969040/1‑151 (MQ=255)
                                            ccAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCCCTTTCGCCCACGCCGGGc                                                                                                      <  2:812954/150‑1 (MQ=255)
                                            ccAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGc                                                                                                      <  1:2151445/150‑1 (MQ=255)
                                            ccAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGc                                                                                                      >  2:158/1‑150 (MQ=255)
                                            ccAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCa                                                                                                     <  1:618335/151‑1 (MQ=255)
                                              aaTCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGc                                                                                                      >  1:489334/1‑148 (MQ=255)
                                              aaTCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGa                                                                                                   <  2:1452975/151‑1 (MQ=255)
                                                 cACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATcc                                                                                                >  1:277906/1‑151 (MQ=255)
                                                     tgatgaTAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATc                                                                                             >  1:1691023/1‑150 (MQ=255)
                                                      gatgatAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATc                                                                                             >  1:2132335/1‑149 (MQ=255)
                                                          atAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGtt                                                                                       <  1:143747/151‑1 (MQ=255)
                                                                aTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTtatat                                                                                   >  2:1377540/1‑149 (MQ=255)
                                                                aTGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAg                                                                                 <  2:1590303/151‑1 (MQ=255)
                                                                 tGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGc                                                                                >  1:2085764/1‑151 (MQ=255)
                                                                  gTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAgag                                                                               <  1:1659134/151‑3 (MQ=255)
                                                                    tCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCgg                                                                              <  1:573365/150‑1 (MQ=255)
                                                                    tCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGt                                                                             >  2:136614/1‑151 (MQ=255)
                                                                    tCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGGGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCgg                                                                              <  1:661839/150‑1 (MQ=255)
                                                                     cATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTc                                                                            >  2:294828/1‑151 (MQ=255)
                                                                     cATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTc                                                                            >  1:2033248/1‑151 (MQ=255)
                                                                     cATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTc                                                                            <  1:16920/151‑1 (MQ=255)
                                                                       tCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGc                                                                          >  1:1826438/1‑151 (MQ=255)
                                                                        ccACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGcc                                                                         >  1:363159/1‑151 (MQ=255)
                                                                              tCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTg                                                                   >  1:1243123/1‑151 (MQ=255)
                                                                               cAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTg                                                                   <  1:281423/150‑1 (MQ=255)
                                                                               cAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTg                                                                   <  2:2111824/150‑1 (MQ=255)
                                                                               cAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGc                                                                  <  2:368717/151‑1 (MQ=255)
                                                                               cAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGc                                                                  >  1:1065134/1‑151 (MQ=255)
                                                                                aGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGc                                                                  >  1:1240413/1‑150 (MQ=255)
                                                                                   gcCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGc                                                              >  1:1675605/1‑151 (MQ=255)
                                                                                    cccGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCg                                                             >  1:1552811/1‑151 (MQ=255)
                                                                                     ccGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCg                                                             >  2:1864034/1‑150 (MQ=255)
                                                                                     ccGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCg                                                             >  1:1619619/1‑150 (MQ=255)
                                                                                          cATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAgcg                                                       <  1:1735241/151‑1 (MQ=255)
                                                                                           aTCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAgcgc                                                      >  1:1606323/1‑151 (MQ=255)
                                                                                               gCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTg                                                  >  1:1151628/1‑151 (MQ=255)
                                                                                                  ttAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCt                                               >  2:542470/1‑151 (MQ=255)
                                                                                                   tAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTc                                              >  1:843368/1‑151 (MQ=255)
                                                                                                    aaTCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCa                                             <  1:1617826/151‑1 (MQ=255)
                                                                                                     aTCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCAc                                            <  1:1462136/151‑1 (MQ=255)
                                                                                                     aTCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCAc                                            >  2:697721/1‑151 (MQ=255)
                                                                                                      tCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACg                                           >  2:706202/1‑151 (MQ=255)
                                                                                                       cAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACgt                                          >  2:983143/1‑151 (MQ=255)
                                                                                                       cAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACgt                                          >  2:1648825/1‑151 (MQ=255)
                                                                                                         gCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACgtgt                                        >  1:855418/1‑151 (MQ=255)
                                                                                                          ccACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACgtgt                                        >  1:1993968/1‑150 (MQ=255)
                                                                                                           cacaGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTAc                                      <  2:2032927/151‑1 (MQ=255)
                                                                                                             caGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAg                                    <  2:2109052/151‑1 (MQ=255)
                                                                                                              aGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAgg                                   <  2:561538/151‑1 (MQ=255)
                                                                                                               gTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgc                                  <  2:21425/151‑1 (MQ=255)
                                                                                                               gTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgc                                  <  2:1721601/151‑1 (MQ=255)
                                                                                                                  ccAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgccgc                               >  1:1267566/1‑151 (MQ=255)
                                                                                                                  ccAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGgccgc                               >  2:790364/1‑151 (MQ=255)
                                                                                                                      gTCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCcac                            >  2:1183545/1‑150 (MQ=255)
                                                                                                                       tCGAGGCGAGGAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACAt                          >  2:1382244/1‑151 (MQ=255)
                                                                                                                          aggcgaggAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGc                       >  1:1766077/1‑151 (MQ=255)
                                                                                                                           ggcgaggAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCTCATCGCt                      <  1:1699541/151‑1 (MQ=255)
                                                                                                                              gaggAAGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGAc                   >  1:1738154/1‑151 (MQ=255)
                                                                                                                                  aaGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGt               >  2:805034/1‑151 (MQ=255)
                                                                                                                                  aaGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGt               >  1:1339805/1‑151 (MQ=255)
                                                                                                                                  aaGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGt               <  1:54119/151‑1 (MQ=255)
                                                                                                                                   aGCTGTTCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTg              >  1:531743/1‑151 (MQ=255)
                                                                                                                                         tCGCGGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGatata         <  2:1722439/150‑1 (MQ=255)
                                                                                                                                          cgcgGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATAtc       >  2:2103772/1‑151 (MQ=255)
                                                                                                                                           gcgGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATAtct      >  2:38637/1‑151 (MQ=255)
                                                                                                                                           gcgGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATAtct      <  1:403392/151‑1 (MQ=255)
                                                                                                                                            cgGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATAtctc     <  1:1042502/151‑1 (MQ=255)
                                                                                                                                            cgGTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATAtctc     <  2:688479/151‑1 (MQ=255)
                                                                                                                                             ggTACAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTcc    <  2:303317/151‑1 (MQ=255)
                                                                                                                                                aCAGTGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCgg  <  2:1688090/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGACTGGCTGATAACAAACAGCACCTTATGCCGGTATACCTCTCCAATCACCTGATGATAAAAATGTTCATCCACCGTCAGCGCCCGGTCATCGGCCTTAATCAGCCACAGTACCAGGTCGAGGCGAGGAAGCTGTTCGCGGTACAGCGCAGCATACTCGGTATCGCGAGCGCCACTTTCGCCCACGCCGGGCAGATCCATCAGCGTTATATAGCGGTCGCCGACTTGCAGGCGAAAGCGCAGTGGCTCACGTGTACAGGCCGCCACATCGCTGACCGGTGATATATCTCCGG  >  NC_000913/268495‑268788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: