Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,398 A→G N54S (AAC→AGC)  yjbQ → thiamine phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,3980AG100.0% 466.8 / NA 122N54S (AAC→AGC) yjbQthiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (60/62);  total (60/62)

Only 100 of 124 total aligned reads displayed.

AAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCC  >  NC_000913/4270250‑4270545
                                                                                                                                                    |                                                                                                                                                   
aaGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCt                                                                                                                                                   <  1:1841323/151‑1 (MQ=255)
aaGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCt                                                                                                                                                   >  2:1388395/1‑151 (MQ=255)
aaGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCt                                                                                                                                                   >  2:899833/1‑151 (MQ=255)
aaGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCt                                                                                                                                                   <  1:308287/151‑1 (MQ=255)
aaGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCt                                                                                                                                                   <  1:447208/151‑1 (MQ=255)
  gACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGc                                                                                                                                                 >  1:1902404/1‑151 (MQ=255)
   aCGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCg                                                                                                                                                <  1:286721/151‑1 (MQ=255)
    cGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCAGACACTTAATGAGAGCTGCGa                                                                                                                                               <  2:2151551/151‑1 (MQ=255)
     gCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGAt                                                                                                                                              >  2:1384613/1‑151 (MQ=255)
     gCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGAt                                                                                                                                              >  1:1939951/1‑151 (MQ=255)
       tCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATcc                                                                                                                                            >  1:908835/1‑151 (MQ=255)
       tCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATcc                                                                                                                                            >  1:1241052/1‑151 (MQ=255)
         aCGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCACCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATccc                                                                                                                                           >  1:1364757/1‑150 (MQ=255)
          cGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCAc                                                                                                                                         <  2:1989702/151‑1 (MQ=255)
          cGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCAc                                                                                                                                         >  2:1606171/1‑151 (MQ=255)
                gcgcCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACg                                                                                                                                   <  1:1392564/151‑1 (MQ=255)
                  gcCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGcc                                                                                                                                 >  1:186478/1‑151 (MQ=255)
                  gcCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGcc                                                                                                                                 <  1:1069474/151‑1 (MQ=255)
                   ccAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGcc                                                                                                                                 >  1:803689/1‑150 (MQ=255)
                   ccAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGcc                                                                                                                                 <  2:477368/150‑1 (MQ=255)
                          tcGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATg                                                                                                                         >  2:101924/1‑151 (MQ=255)
                           cGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTATTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGAc                                                                                                                            <  2:732265/147‑1 (MQ=255)
                           cGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACa                                                                                                                           <  1:1386034/148‑1 (MQ=255)
                           cGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATgg                                                                                                                        >  2:760887/1‑151 (MQ=255)
                                   tCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGttt                                                                                                                 <  1:663611/150‑1 (MQ=255)
                                   tCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGttt                                                                                                                 <  1:1947073/150‑1 (MQ=255)
                                       cTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTtcc                                                                                                            >  1:124518/1‑151 (MQ=255)
                                       cTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTtcc                                                                                                            >  1:1265537/1‑151 (MQ=255)
                                         ggTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTtcctc                                                                                                          <  2:1505133/151‑1 (MQ=255)
                                         ggTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTtcc                                                                                                            <  2:1872009/149‑1 (MQ=255)
                                           tAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCg                                                                                                        >  1:949541/1‑151 (MQ=255)
                                           tAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCg                                                                                                        >  1:59277/1‑151 (MQ=255)
                                             aCGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCa                                                                                                      <  2:618130/151‑1 (MQ=255)
                                               ggATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCAcc                                                                                                    <  2:1775088/151‑1 (MQ=255)
                                                   gAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGtt                                                                                                 <  2:832739/150‑1 (MQ=255)
                                                   gAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGtt                                                                                                 <  2:892534/150‑1 (MQ=255)
                                                   gAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTc                                                                                                <  2:1134256/151‑1 (MQ=255)
                                                    aaaTTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTcc                                                                                               <  2:1842491/151‑1 (MQ=255)
                                                      aTTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCg                                                                                             >  1:1677453/1‑151 (MQ=255)
                                                      aTTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCg                                                                                             <  2:1009468/151‑1 (MQ=255)
                                                      aTTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCg                                                                                             <  1:1721520/151‑1 (MQ=255)
                                                        tCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGAc                                                                                           >  2:510022/1‑151 (MQ=255)
                                                        tCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGAc                                                                                           >  1:820755/1‑151 (MQ=255)
                                                           gAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAAc                                                                                        >  2:225072/1‑151 (MQ=255)
                                                             aTCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACgg                                                                                      >  2:1795907/1‑151 (MQ=255)
                                                               cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGaa                                                                                    <  1:954120/151‑1 (MQ=255)
                                                               cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGaa                                                                                    <  2:1604176/151‑1 (MQ=255)
                                                               cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGaa                                                                                    <  1:1820530/151‑1 (MQ=255)
                                                               cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGa                                                                                     <  1:1419652/150‑1 (MQ=255)
                                                               cAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGa                                                                                     <  2:1939913/150‑1 (MQ=255)
                                                                aGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGaa                                                                                    >  1:885379/1‑150 (MQ=255)
                                                                   tggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAAtt                                                                                 >  1:1640051/1‑150 (MQ=255)
                                                                    ggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAAtt                                                                                 <  2:1986406/149‑1 (MQ=255)
                                                                    ggctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTa                                                                                <  2:1238164/150‑1 (MQ=255)
                                                                     gctgATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATg                                                                              >  1:105877/1‑151 (MQ=255)
                                                                        gATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAg                                                                            <  1:1906478/150‑1 (MQ=255)
                                                                             gCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGAc                                                                      >  2:252679/1‑151 (MQ=255)
                                                                              ccGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACt                                                                     >  1:2069035/1‑151 (MQ=255)
                                                                                   cgTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATg                                                                  >  2:1136215/1‑149 (MQ=255)
                                                                                       aaCATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAggg                                                              >  2:1199709/1‑149 (MQ=255)
                                                                                         cATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCa                                                          <  2:235477/151‑1 (MQ=255)
                                                                                          aTCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAg                                                         <  1:426593/151‑1 (MQ=255)
                                                                                           tCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGc                                                           >  1:983322/1‑148 (MQ=255)
                                                                                            cGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGa                                                        <  1:492360/150‑1 (MQ=255)
                                                                                            cGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGAc                                                       >  1:919345/1‑151 (MQ=255)
                                                                                            cGGCTTACTGAATCTGTTGCTGCAACATGCCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGa                                                        <  2:1745369/150‑1 (MQ=255)
                                                                                             ggCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACg                                                      >  2:1959436/1‑151 (MQ=255)
                                                                                                ttACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGat                                                    >  2:1448687/1‑150 (MQ=255)
                                                                                                 tACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGat                                                    <  2:93871/149‑1 (MQ=255)
                                                                                                  aCTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGatat                                                  <  2:1447953/150‑1 (MQ=255)
                                                                                                    tGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGcc                                               >  1:728584/1‑151 (MQ=255)
                                                                                                      cATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCtt                                             <  2:954972/151‑1 (MQ=255)
                                                                                                      cATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCtt                                             >  2:1327096/1‑151 (MQ=255)
                                                                                                       aTCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTtc                                            >  1:1477439/1‑151 (MQ=255)
                                                                                                       aTCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTtc                                            >  1:839622/1‑151 (MQ=255)
                                                                                                          tGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTtctc                                          >  1:1657007/1‑150 (MQ=255)
                                                                                                          tGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTtctc                                          >  1:1231027/1‑150 (MQ=255)
                                                                                                           gTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTtct                                           <  1:1822381/148‑1 (MQ=255)
                                                                                                           gTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCa                                         <  1:1155239/150‑1 (MQ=255)
                                                                                                                        aCCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCa                            <  1:982738/150‑1 (MQ=255)
                                                                                                                        aCCTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCa                            <  2:585099/150‑1 (MQ=255)
                                                                                                                         ccTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCaa                           <  2:1705441/150‑1 (MQ=255)
                                                                                                                          cTCCGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCt                              <  2:1347659/146‑1 (MQ=255)
                                                                                                                             cGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTg                      >  2:2078204/1‑151 (MQ=255)
                                                                                                                             cGCCTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTg                      <  2:264794/151‑1 (MQ=255)
                                                                                                                               ccTCTCTGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTggg                    >  2:1487897/1‑151 (MQ=255)
                                                                                                                                ctctctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTggg                    >  1:1210596/1‑150 (MQ=255)
                                                                                                                                ctctctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTggg                    >  1:1537264/1‑150 (MQ=255)
                                                                                                                                   tctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAAc                 >  2:854587/1‑150 (MQ=255)
                                                                                                                                    ctGACACTTAATGAGAGCTGCGATCCCGCCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGaa                  <  1:111124/148‑1 (MQ=255)
                                                                                                                                    ctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAAc                 <  2:1119672/149‑1 (MQ=255)
                                                                                                                                    ctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACAt               >  2:617618/1‑151 (MQ=255)
                                                                                                                                    ctGACACTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACAt               >  2:442618/1‑151 (MQ=255)
                                                                                                                                       acacTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGc            >  1:1373604/1‑151 (MQ=255)
                                                                                                                                       acacTTAATGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGc            >  2:182516/1‑151 (MQ=255)
                                                                                                                                               tGAGAGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTAtt     >  2:680144/1‑150 (MQ=255)
                                                                                                                                                gagaGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGc   <  2:839611/151‑1 (MQ=255)
                                                                                                                                                gagaGCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGc   <  2:1887176/151‑1 (MQ=255)
                                                                                                                                                 agagCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGcc  >  1:1538042/1‑151 (MQ=255)
                                                                                                                                                 agagCTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGcc  >  2:279210/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGACGCTCACGCTTAGCGCCAAATCTCGTGGGTTTCATCTGGTAACGGATGAAATTCTGAATCAGCTGGCTGATATGCCGCGCGTTAACATCGGCTTACTGCATCTGTTGCTGCAACATACCTCCGCCTCTCTGACACTTAATGAGAACTGCGATCCCACCGTACGCCACGACATGGAGCGTTTTTTCCTCCGCACCGTTCCCGACAACGGAAATTATGAGCATGACTATGAGGGAGCAGACGATATGCCTTCTCATATCAAATCCTCAATGCTGGGAACATCGCTTGTATTGCC  >  NC_000913/4270250‑4270545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: