Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,529,852 A→G V131A (GTG→GCG)  sgcC ← putative PTS system EIIC permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,529,8520AG100.0% 447.6 / NA 120V131A (GTG→GCG) sgcCputative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/72);  total (48/72)

Only 100 of 124 total aligned reads displayed.

CCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGG  >  NC_000913/4529705‑4529999
                                                                                                                                                   |                                                                                                                                                   
ccGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGAccgccg                                                                                                                                                  >  2:2013646/1‑151 (MQ=255)
ccGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGAccgccg                                                                                                                                                  >  1:1937472/1‑151 (MQ=255)
 cGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGt                                                                                                                                                 >  2:1936496/1‑151 (MQ=255)
 cGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGt                                                                                                                                                 >  1:20069/1‑151 (MQ=255)
      ccTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCg                                                                                                                                            >  1:1215538/1‑151 (MQ=255)
      ccTTGCGGTATCGAAATACCTCCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTAccc                                                                                                                                             <  1:968770/150‑1 (MQ=255)
        ttGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGt                                                                                                                                           >  2:1396046/1‑150 (MQ=255)
          gCGGTATCGAAATACCTCCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCGGGACCGCCGTACCCGTaa                                                                                                                                         <  2:1026980/150‑1 (MQ=255)
                  gAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGt                                                                                                                                >  2:1784856/1‑151 (MQ=255)
                  gAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGt                                                                                                                                >  1:1386816/1‑151 (MQ=255)
                   aaaTCCCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTa                                                                                                                                  <  1:242607/148‑1 (MQ=255)
                      tACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGcc                                                                                                                             >  1:1488093/1‑150 (MQ=255)
                       aCCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGcc                                                                                                                             >  1:576921/1‑149 (MQ=255)
                        ccTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGt                                                                                                                          >  2:1421223/1‑151 (MQ=255)
                          ttCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTa                                                                                                                         >  2:1525321/1‑150 (MQ=255)
                            ccAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACCCCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTa                                                                                                                         <  1:238541/148‑1 (MQ=255)
                            ccAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAAtt                                                                                                                      >  2:1162446/1‑151 (MQ=255)
                            ccAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGCCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACCCCATAAACCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTa                                                                                                                         <  2:858608/148‑1 (MQ=255)
                               gCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGt                                                                                                                    >  1:98140/1‑150 (MQ=255)
                                cccGACGATGTTCTGTCCCGTTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGATTGGCAGATTGCCCCCAGCACACCATAAATCGGGCTACCGGTCATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGt                                                                                                                    <  1:1164514/149‑1 (MQ=255)
                                cccGACGATGTTCTGTACCGGTTTTGCCGCCCAGTCGGCCATTTTTAACGATAGCGCCGCAGGGCAGTTTGCCCCCAGCACCCCATAAACCAGGCTACCGTTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGt                                                                                                                    <  2:1790256/149‑1 (MQ=255)
                                         gTTCTGTACCGGTTTGGCCGTCCAGTCGGCCTTTTTAAACGATGGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATAAGGCTACCGGTTATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATc                                                                                                             <  2:1508424/147‑1 (MQ=255)
                                         gTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACg                                                                                                         >  2:157577/1‑151 (MQ=255)
                                          ttCTGTACCCTTTTTGCCGCCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTCCCCCCAGCCCACCATAAATCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACg                                                                                                         <  2:960247/150‑1 (MQ=255)
                                          ttCTGTACCCGTTTTGCCGCCCAGTCGGCCATTTTTAACGAAAGCCCCGCATGGCAGATTGCCCCCAGCACCCCATAAACCAGGCTACCGGTAATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGAc                                                                                                          <  2:78069/149‑1 (MQ=255)
                                             tGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGt                                                                                                        <  2:439227/148‑1 (MQ=255)
                                             tGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTc                                                                                                      >  1:131176/1‑150 (MQ=255)
                                             tGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTc                                                                                                      >  1:491069/1‑150 (MQ=255)
                                              gtccccTTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGtt                                                                                                       <  1:1447114/145‑1 (MQ=255)
                                              gTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCa                                                                                                     <  1:1007466/150‑1 (MQ=255)
                                              gTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCAt                                                                                                    <  1:1958535/151‑1 (MQ=255)
                                                 cccTTTTTGCCGTCCATTCGGCCATTTTTAACGATACCGCCGCATGGCAGTTTGCCCCCAGCACACCATAAATCAGGCTACCGTTCATAAGCTGAACCGCCATACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGt                                                                                                        <  2:1128691/144‑1 (MQ=255)
                                                 cccGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTCCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTc                                                                                                      <  2:6839/146‑1 (MQ=255)
                                                   cGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGttt                                                                                               >  2:883677/1‑151 (MQ=255)
                                                    gTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGtttt                                                                                              >  1:2037097/1‑151 (MQ=255)
                                                     ttttGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCATATTGCCCCCAGCACACCAAAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGttt                                                                                               <  2:1693968/149‑1 (MQ=255)
                                                     ttttGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTg                                                                                             >  2:1259811/1‑151 (MQ=255)
                                                      tttGCCGTCAAGTCGGCCATTTTTAACGATAGCGCCGCAGGGTAGATTCCCCCCAGCACACCAAAAACCAGGCTAACGGTTATAAGCTGAACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTg                                                                                             <  2:551532/150‑1 (MQ=255)
                                                        tGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTc                                                                                          >  1:902019/1‑151 (MQ=255)
                                                         gCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGt                                                                                           <  1:1542611/149‑1 (MQ=255)
                                                          ccGTCCAGTCGGCCTTTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTAATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAg                                                                                        <  2:1221449/151‑1 (MQ=255)
                                                          ccGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTc                                                                                          >  1:1990194/1‑149 (MQ=255)
                                                              ccAGTCGGCCATTTTTAACGTTAGCGCCGCAGGGCAGATTGCCCCCAGCACACCATAAATCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGAc                                                                                      <  1:456114/149‑1 (MQ=255)
                                                              ccAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACgg                                                                                    >  1:1381867/1‑151 (MQ=255)
                                                                aGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGt                                                                                  >  2:1705262/1‑151 (MQ=255)
                                                                 ggcGGCCATTTTTAACGAAGGCGCCGCATGGGAGTTTGCCCCCAGCACACCAAAAACCAGGCTGCCGGTCAAAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTg                                                                                 <  1:547849/149‑1 (MQ=255)
                                                                    ggCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGAcc                                                                              >  2:1861561/1‑151 (MQ=255)
                                                                     gCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGAcc                                                                              >  2:199361/1‑150 (MQ=255)
                                                                         tttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGc                                                                           <  1:39973/149‑1 (MQ=255)
                                                                         tttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCa                                                                          <  1:876524/150‑1 (MQ=255)
                                                                         tttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCa                                                                          <  1:515601/150‑1 (MQ=255)
                                                                          ttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACCCCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCAt                                                                         <  1:144437/150‑1 (MQ=255)
                                                                             tAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCg                                                                     >  1:793715/1‑151 (MQ=255)
                                                                              aaCGATAGCGCCGCATGGCAGATTCCCCCCAGCACACCATAAACCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCg                                                                     <  2:1138470/150‑1 (MQ=255)
                                                                              aaCGATAGCGCCGCATGGCAATTTCCCCCCAGCACCCCAAAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGc                                                                      <  2:913707/149‑1 (MQ=255)
                                                                               aCGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGAc                                                                   >  2:709686/1‑151 (MQ=255)
                                                                                cGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACa                                                                  <  1:272393/151‑1 (MQ=255)
                                                                                 gATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACAt                                                                 <  2:1627629/151‑1 (MQ=255)
                                                                                  aTAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACAtt                                                                >  2:40529/1‑151 (MQ=255)
                                                                                   tAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTg                                                               >  2:1051159/1‑151 (MQ=255)
                                                                                   tAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTg                                                               >  1:1176673/1‑151 (MQ=255)
                                                                                     gcgcCGCATGGCAGATTGCCCCCAGCACCCCATAAATCAGGCTACCGATCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTga                                                              <  1:432980/150‑1 (MQ=255)
                                                                                        ccgcATGGCAGATTGCCCCCAGCACACCATAAACCAGGCTACCGGTCATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGagaag                                                          <  2:1993899/151‑1 (MQ=255)
                                                                                           cATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAgaagaa                                                        >  1:1788386/1‑150 (MQ=255)
                                                                                           cATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGaaa                                                       <  1:595670/151‑1 (MQ=255)
                                                                                                 aGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATa                                                   <  1:2019612/149‑1 (MQ=255)
                                                                                                 aGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAAc                                                 >  2:385028/1‑151 (MQ=255)
                                                                                                        ccccAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGAt                                           >  2:649760/1‑150 (MQ=255)
                                                                                                        ccccAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATa                                          <  1:1996122/151‑1 (MQ=255)
                                                                                                         cccAGCACACAAAAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATaa                                         <  2:1874693/151‑1 (MQ=255)
                                                                                                          ccAGCACCCCATAAATCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAGTGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAAt                                        <  2:965151/151‑1 (MQ=255)
                                                                                                            aGCACACCAAAAATCAGGCTACCGGTAATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAAt                                        <  1:208355/149‑1 (MQ=255)
                                                                                                              cccacCATAAAGCAGCCTGCCGGTCATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCa                                      <  1:1653306/147‑1 (MQ=255)
                                                                                                               acacCAAAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATg                                    <  2:1973515/150‑1 (MQ=255)
                                                                                                                  ccATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGgcg                                 <  1:1162864/150‑1 (MQ=255)
                                                                                                                  ccATAAATCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGgcg                                 <  1:60412/150‑1 (MQ=255)
                                                                                                                  ccATAAATCAGGCTACCGGTCATAAGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGgct                                 <  2:815880/150‑2 (MQ=255)
                                                                                                                      aaaTCAGGCTCCCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCaa                             <  2:1590311/150‑1 (MQ=255)
                                                                                                                      aaaTCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCaa                             <  1:407828/150‑1 (MQ=255)
                                                                                                                      aaaTCAGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAAt                            <  2:20069/151‑1 (MQ=255)
                                                                                                                           aGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCt                        <  1:1559646/150‑1 (MQ=255)
                                                                                                                           aGGCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTg                       >  2:1934961/1‑151 (MQ=255)
                                                                                                                            ggCTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGt                      <  1:14884/151‑1 (MQ=255)
                                                                                                                              cTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTc                     <  2:25911/150‑1 (MQ=255)
                                                                                                                              cTACCGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCg                    >  1:107272/1‑151 (MQ=255)
                                                                                                                                 ccGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCAt                 >  1:1542752/1‑151 (MQ=255)
                                                                                                                                 ccGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCAt                 >  2:2087892/1‑151 (MQ=255)
                                                                                                                                  cGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCAt                 >  2:1652378/1‑150 (MQ=255)
                                                                                                                                  cGGTCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCAt                 >  2:734159/1‑150 (MQ=255)
                                                                                                                                     tCATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAAcc             >  2:144157/1‑151 (MQ=255)
                                                                                                                                      cATAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAAcc             <  1:1936534/150‑1 (MQ=255)
                                                                                                                                       aTAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCaa           <  1:1882762/151‑1 (MQ=255)
                                                                                                                                       aTAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCa            >  1:924666/1‑150 (MQ=255)
                                                                                                                                        tAAGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAAc          >  2:755260/1‑151 (MQ=255)
                                                                                                                                         aaGCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACg         <  2:1937434/151‑1 (MQ=255)
                                                                                                                                         aaGCGGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACg         <  2:633052/151‑1 (MQ=255)
                                                                                                                                           gCTGGACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGc        <  1:855333/150‑1 (MQ=255)
                                                                                                                                              ggACCGCCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCg      <  2:1408836/149‑1 (MQ=255)
                                                                                                                                                accgccGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAgg  >  2:68739/1‑151 (MQ=255)
                                                                                                                                                accgccGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAg   <  2:62323/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGCCAATGGCTGTCGCATAACCAACGCCGGAGG  >  NC_000913/4529705‑4529999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑