Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 384,316 C→G A101P (GCC→CCC)  yaiS ← putative PIG‑L family deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913384,3160CG99.2% 503.7 / ‑3.3 132A101P (GCC→CCC) yaiSputative PIG‑L family deacetylase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (58/73);  total (59/73)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 135 total aligned reads displayed.

CCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGTC  >  NC_000913/384168‑384461
                                                                                                                                                    |                                                                                                                                                 
 cGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAgggggg                                                                                                                                                 >  2:908402/1‑150 (MQ=255)
 cGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAgggggg                                                                                                                                                 >  1:898934/1‑150 (MQ=255)
  gagaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGa                                                                                                                                                >  2:1438882/1‑150 (MQ=255)
  gagaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGa                                                                                                                                                >  2:470475/1‑150 (MQ=255)
   agaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGaa                                                                                                                                               >  2:1738488/1‑150 (MQ=255)
   agaATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGaa                                                                                                                                               >  2:1580571/1‑150 (MQ=255)
      aTTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatca                                                                                                                                           <  1:504869/151‑1 (MQ=255)
      aTTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatc                                                                                                                                            >  2:834157/1‑150 (MQ=255)
      aTTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatc                                                                                                                                            >  1:1595995/1‑150 (MQ=255)
       tttGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatcat                                                                                                                                          >  1:722457/1‑151 (MQ=255)
         tGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatcata                                                                                                                                         <  2:509322/150‑1 (MQ=255)
          gCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAatcatatc                                                                                                                                       >  1:2125172/1‑151 (MQ=255)
           cGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGACATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAatcatatc                                                                                                                                       >  2:141934/1‑150 (MQ=255)
              aaTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTg                                                                                                                                   <  1:1617598/151‑1 (MQ=255)
              aaTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTg                                                                                                                                   <  2:704156/151‑1 (MQ=255)
              aaTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTg                                                                                                                                   <  1:1674968/151‑1 (MQ=255)
               aTAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGa                                                                                                                                  <  1:1750189/151‑1 (MQ=255)
                 aGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAg                                                                                                                                 <  2:407537/150‑1 (MQ=255)
                 aGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGc                                                                                                                                <  1:194972/151‑1 (MQ=255)
                     cGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGt                                                                                                                             <  1:238911/150‑1 (MQ=255)
                      ggcaggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTaa                                                                                                                           <  1:1484032/151‑1 (MQ=255)
                      ggcaggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTaa                                                                                                                           <  1:217122/151‑1 (MQ=255)
                      ggcaggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTACAGGGGGGAAATCATATCATTGAGCTGTaa                                                                                                                           <  1:910280/151‑1 (MQ=255)
                       gcaggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTaa                                                                                                                           >  1:748913/1‑150 (MQ=255)
                        caggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCGGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAAt                                                                                                                         <  2:1276634/151‑1 (MQ=255)
                         aggcaACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATg                                                                                                                        >  2:417114/1‑151 (MQ=255)
                            caACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAgc                                                                                                                     >  1:936015/1‑151 (MQ=255)
                             aaCCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAgcg                                                                                                                    >  1:668653/1‑151 (MQ=255)
                             aaCCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAgcg                                                                                                                    >  1:422874/1‑151 (MQ=255)
                               ccATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGcgcg                                                                                                                  >  1:982025/1‑151 (MQ=255)
                                   tGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGgtg                                                                                                              >  1:890889/1‑151 (MQ=255)
                                   tGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGgtg                                                                                                              >  1:2115802/1‑151 (MQ=255)
                                    gAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGgtgt                                                                                                             >  2:78429/1‑151 (MQ=255)
                                      aGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCa                                                                                                           <  1:1245569/151‑1 (MQ=255)
                                       gCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAg                                                                                                          <  1:851643/151‑1 (MQ=255)
                                         ttGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGc                                                                                                         >  1:1397067/1‑150 (MQ=255)
                                         ttGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGc                                                                                                         >  1:1482657/1‑150 (MQ=255)
                                           gATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCaaa                                                                                                      <  1:1696767/151‑1 (MQ=255)
                                              aaaCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAAtt                                                                                                   >  2:817856/1‑151 (MQ=255)
                                              aaaCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAAtt                                                                                                   >  1:1913627/1‑151 (MQ=255)
                                               aaCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTa                                                                                                  <  1:1454109/151‑1 (MQ=255)
                                                aCAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTa                                                                                                  >  1:179577/1‑150 (MQ=255)
                                                  aGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGa                                                                                               <  1:1069734/151‑1 (MQ=255)
                                                      aGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGa                                                                                            >  2:1081759/1‑150 (MQ=255)
                                                      aGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGa                                                                                            >  1:1190344/1‑150 (MQ=255)
                                                          gatCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAg                                                                                        <  2:319196/150‑1 (MQ=255)
                                                             ccTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGttt                                                                                     <  1:582451/150‑1 (MQ=255)
                                                             ccTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGttt                                                                                     <  1:966651/150‑1 (MQ=255)
                                                             ccTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTtg                                                                                    <  2:1242701/151‑1 (MQ=255)
                                                                gATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTtgg                                                                                   <  1:911372/149‑1 (MQ=255)
                                                                 aTGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTtggtgg                                                                                <  1:735644/151‑1 (MQ=255)
                                                                    gCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCAc                                                                             <  2:1303168/151‑1 (MQ=255)
                                                                      ggTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCAccc                                                                           <  2:2114436/151‑1 (MQ=255)
                                                                       gTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCt                                                                          >  1:656851/1‑151 (MQ=255)
                                                                             atcatGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATc                                                                    >  1:980011/1‑151 (MQ=255)
                                                                                atgcatgGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCttt                                                                 >  2:1383919/1‑151 (MQ=255)
                                                                                      ggTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGc                                                            <  1:897934/150‑1 (MQ=255)
                                                                                      ggTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCg                                                           <  1:1915269/151‑1 (MQ=255)
                                                                                      ggTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCg                                                           >  1:545054/1‑151 (MQ=255)
                                                                                         atataCCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGtt                                                         <  2:827078/150‑1 (MQ=255)
                                                                                           ataCCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTgcg                                                      >  1:2132265/1‑151 (MQ=255)
                                                                                           ataCCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTgc                                                       <  2:2108075/150‑1 (MQ=255)
                                                                                              cccGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGcgcg                                                    >  1:164006/1‑150 (MQ=255)
                                                                                                cGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAtt                                                 <  2:151662/151‑1 (MQ=255)
                                                                                                 gCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAtt                                                 <  2:523966/150‑1 (MQ=255)
                                                                                                    tGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttcttc                                             >  1:567464/1‑151 (MQ=255)
                                                                                                     gATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttcttc                                             >  1:809623/1‑150 (MQ=255)
                                                                                                     gATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttcttc                                             <  1:1080826/150‑1 (MQ=255)
                                                                                                     gATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGAttcttc                                             >  2:1813034/1‑150 (MQ=255)
                                                                                                      aTTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCAt                                           <  1:1354557/151‑1 (MQ=255)
                                                                                                        ttCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATg                                          <  1:417117/150‑1 (MQ=255)
                                                                                                          cAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGcg                                       >  1:630538/1‑151 (MQ=255)
                                                                                                          cAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGc                                        <  2:451744/150‑1 (MQ=255)
                                                                                                          cAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGc                                        <  1:315497/150‑1 (MQ=255)
                                                                                                             cATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGcgatc                                    >  2:1562059/1‑151 (MQ=255)
                                                                                                              aTCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGcgatcg                                   <  2:1450832/151‑1 (MQ=255)
                                                                                                                cAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGcgatcgat                                 >  2:1168757/1‑151 (MQ=255)
                                                                                                                    aGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGattatt                             >  1:2165171/1‑151 (MQ=255)
                                                                                                                    aGGAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGattatt                             <  2:1232303/151‑1 (MQ=255)
                                                                                                                     ggAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTc                            <  2:1293301/151‑1 (MQ=255)
                                                                                                                      gAATTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGTGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTcc                           >  2:216614/1‑151 (MQ=255)
                                                                                                                       aaTTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCa                          <  1:1010526/151‑1 (MQ=255)
                                                                                                                        aTTTGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCAt                         >  2:1142054/1‑151 (MQ=255)
                                                                                                                           tGATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCt                       <  1:1212357/150‑1 (MQ=255)
                                                                                                                            gATTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCtg                      <  2:1482631/150‑1 (MQ=255)
                                                                                                                             aTTTTTAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCtgtg                    <  2:92976/151‑1 (MQ=255)
                                                                                                                                tttAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGcc                  >  2:785895/1‑150 (MQ=255)
                                                                                                                                 ttAATGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCa                 <  2:1426230/150‑1 (MQ=255)
                                                                                                                                   aaTGATGTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCag                >  1:1656187/1‑149 (MQ=255)
                                                                                                                                     tgatgTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGtt            >  2:396114/1‑151 (MQ=255)
                                                                                                                                      gatgTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGtt            <  2:2156189/150‑1 (MQ=255)
                                                                                                                                         gTCTTCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGcc         <  1:79062/150‑1 (MQ=255)
                                                                                                                                             tCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGt      <  2:604755/149‑1 (MQ=255)
                                                                                                                                             tCCAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTa     <  1:190397/150‑1 (MQ=255)
                                                                                                                                              ccAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGt   <  1:2039069/151‑1 (MQ=255)
                                                                                                                                               cAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGtc  >  1:1991008/1‑151 (MQ=255)
                                                                                                                                               cAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGtc  <  1:1338355/151‑1 (MQ=255)
                                                                                                                                               cAGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGtc  >  1:670193/1‑151 (MQ=255)
                                                                                                                                                aGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGtc  >  2:437976/1‑150 (MQ=255)
                                                                                                                                                aGGGGGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGtc  >  2:1891516/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCGAGAATTTGCGGAATAGTTCGGCAGGCAACCATTGAAGCTTGATAAACAGCAAGATGATCCTGATGGCGGTCGGCATCATGCATGGTATATACCCGCATGATTTCAACATCAGAAGGAATTTGATTTTTAATGATGTCTTCCAGGGCGGAAATCATATCATTGAGCTGTAAATGAGCGCGGGTGTCAGCAAAATTAAGATGAATAGTTTGGTGGCACCCTAATATCTTTAGGGCGTTGCGCGATTCTTCATGGCGATCGATTATTCCATCTGTGCCAGAGTTGCCGGTAGTC  >  NC_000913/384168‑384461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑