Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 471,711 A→G N359S (AAC→AGC)  mdlB → putative multidrug ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913471,7110AG100.0% 568.3 / NA 141N359S (AAC→AGC) mdlBputative multidrug ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (60/81);  total (60/81)

Only 100 of 144 total aligned reads displayed.

AGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCT  >  NC_000913/471564‑471858
                                                                                                                                                   |                                                                                                                                                   
aGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCAt                                                                                                                                                  >  1:1166180/1‑151 (MQ=255)
aGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCAt                                                                                                                                                  <  2:1265867/151‑1 (MQ=255)
aGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCAt                                                                                                                                                  <  2:1608538/151‑1 (MQ=255)
  gCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCAtt                                                                                                                                                 >  1:684896/1‑150 (MQ=255)
   cTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTa                                                                                                                                                >  2:498314/1‑150 (MQ=255)
   cTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTa                                                                                                                                                >  1:830800/1‑150 (MQ=255)
       tgttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAAtctc                                                                                                                                           >  1:628770/1‑151 (MQ=255)
           gCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCtg                                                                                                                                       >  2:142262/1‑151 (MQ=255)
              ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCtgt                                                                                                                                      <  1:1908749/149‑1 (MQ=255)
              ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCtgt                                                                                                                                      <  2:1699124/149‑1 (MQ=255)
               gTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGcc                                                                                                                                   <  2:318562/151‑1 (MQ=255)
                 gAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCtt                                                                                                                                 >  2:1294511/1‑151 (MQ=255)
                 gAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCtt                                                                                                                                 <  2:828410/151‑1 (MQ=255)
                  aGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTc                                                                                                                                <  1:1825919/151‑1 (MQ=255)
                  aGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTc                                                                                                                                >  1:906002/1‑151 (MQ=255)
                   gcgcGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTcg                                                                                                                               <  2:1488614/151‑1 (MQ=255)
                   gcgcGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTcg                                                                                                                               <  2:906063/151‑1 (MQ=255)
                   gcgcGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTcg                                                                                                                               <  2:414364/151‑1 (MQ=255)
                     gcgTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTcgcg                                                                                                                             <  1:2082127/151‑1 (MQ=255)
                     gcgTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTcgc                                                                                                                              <  2:2051056/150‑1 (MQ=255)
                           ttGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAAtt                                                                                                                        <  1:1755104/150‑1 (MQ=255)
                              aaCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTtgt                                                                                                                    >  2:688879/1‑151 (MQ=255)
                               aCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTtgtg                                                                                                                   <  2:227997/151‑1 (MQ=255)
                               aCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTtgtg                                                                                                                   <  2:798519/151‑1 (MQ=255)
                               aCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTtgtg                                                                                                                   <  2:289109/151‑1 (MQ=255)
                                  gATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGgc                                                                                                                 <  2:2159047/150‑1 (MQ=255)
                                           acCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCggg                                                                                                       >  2:1725181/1‑151 (MQ=255)
                                             cgcgCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGc                                                                                                      >  2:291202/1‑150 (MQ=255)
                                              gcgcCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCAt                                                                                                    >  2:751299/1‑151 (MQ=255)
                                              gcgcCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCAt                                                                                                    <  1:250789/151‑1 (MQ=255)
                                               cgcCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATa                                                                                                   <  1:1690838/151‑1 (MQ=255)
                                                gcCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATAc                                                                                                  >  2:1956067/1‑151 (MQ=255)
                                                   aGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCgg                                                                                               <  1:810016/151‑1 (MQ=255)
                                                   aGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCgg                                                                                               >  1:973603/1‑151 (MQ=255)
                                                     cAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCa                                                                                             <  1:2070075/151‑1 (MQ=255)
                                                           ggCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCa                                                                                       <  2:485006/151‑1 (MQ=255)
                                                            gCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa                                                                                      <  1:687567/151‑1 (MQ=255)
                                                             cAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaa                                                                                      >  2:1088100/1‑150 (MQ=255)
                                                               atgatgATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCaaa                                                                                     >  2:1095032/1‑149 (MQ=255)
                                                                  atgatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAc                                                                                >  1:760166/1‑151 (MQ=255)
                                                                  atgatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAc                                                                                >  2:1401833/1‑151 (MQ=255)
                                                                   tgatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc                                                                               >  2:1628330/1‑151 (MQ=255)
                                                                   tgatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAcc                                                                               >  1:136557/1‑151 (MQ=255)
                                                                    gatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAccc                                                                              <  2:1384672/151‑1 (MQ=255)
                                                                    gatCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCAccc                                                                              >  2:1865086/1‑151 (MQ=255)
                                                                     atCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCt                                                                             <  2:275618/151‑1 (MQ=255)
                                                                      tCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTc                                                                            >  1:491282/1‑151 (MQ=255)
                                                                      tCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTc                                                                            >  1:1620748/1‑151 (MQ=255)
                                                                       cGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCg                                                                           <  2:1763607/151‑1 (MQ=255)
                                                                       cGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCg                                                                           >  2:954003/1‑151 (MQ=255)
                                                                           cGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAg                                                                       <  2:203560/151‑1 (MQ=255)
                                                                            gTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt                                                                      >  1:1243789/1‑151 (MQ=255)
                                                                            gTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGt                                                                      >  2:1646116/1‑151 (MQ=255)
                                                                              tACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGtt                                                                     <  1:1258857/150‑1 (MQ=255)
                                                                                cAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAt                                                                  <  1:199538/151‑1 (MQ=255)
                                                                                 agagTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTAtt                                                                 >  2:1900098/1‑151 (MQ=255)
                                                                                  gagTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTg                                                                <  1:1850542/151‑1 (MQ=255)
                                                                                    gTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGAt                                                              <  1:903445/151‑1 (MQ=255)
                                                                                      ggCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATg                                                             <  1:1436634/150‑1 (MQ=255)
                                                                                      ggCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATg                                                             <  1:9507/150‑1 (MQ=255)
                                                                                          ccATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTa                                                        <  1:159722/151‑1 (MQ=255)
                                                                                            aTCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTAtt                                                      >  2:945581/1‑151 (MQ=255)
                                                                                              cGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTAc                                                    >  1:2007718/1‑151 (MQ=255)
                                                                                                aaGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTAccc                                                  <  1:1099078/151‑1 (MQ=255)
                                                                                                aaGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTAccc                                                  <  2:1820311/151‑1 (MQ=255)
                                                                                                aaGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTAccc                                                  >  2:669317/1‑151 (MQ=255)
                                                                                                    cGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTa                                              <  2:1986513/151‑1 (MQ=255)
                                                                                                     gATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTa                                              >  2:1673645/1‑150 (MQ=255)
                                                                                                      aTAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAAc                                            >  2:1407355/1‑151 (MQ=255)
                                                                                                         aCGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACgg                                          >  2:572457/1‑150 (MQ=255)
                                                                                                         aCGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACgg                                          >  1:788434/1‑150 (MQ=255)
                                                                                                           gtgtCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAg                                       <  2:1749760/151‑1 (MQ=255)
                                                                                                             gtCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGt                                     <  2:2080893/151‑1 (MQ=255)
                                                                                                              tCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTg                                    >  2:1568695/1‑151 (MQ=255)
                                                                                                               cATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTg                                    <  1:1650700/150‑1 (MQ=255)
                                                                                                                aTTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTgag                                  <  1:1375296/151‑1 (MQ=255)
                                                                                                                aTTTGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTgag                                  <  1:260524/151‑1 (MQ=255)
                                                                                                                  ttGCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTg                                    >  2:1850505/1‑147 (MQ=255)
                                                                                                                    gCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGAtt                               <  2:756733/150‑1 (MQ=255)
                                                                                                                    gCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTc                              <  2:783668/151‑1 (MQ=255)
                                                                                                                    gCTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTc                              <  1:503921/151‑1 (MQ=255)
                                                                                                                     cTTATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCg                             >  1:1866728/1‑151 (MQ=255)
                                                                                                                       tATCGCGATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCg                             <  2:261274/149‑1 (MQ=255)
                                                                                                                          cgcgATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTg                        >  2:503540/1‑151 (MQ=255)
                                                                                                                            cgATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGAt                      >  2:1958365/1‑151 (MQ=255)
                                                                                                                            cgATGACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGAt                      >  2:1499856/1‑151 (MQ=255)
                                                                                                                                gACAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGgt                   <  2:896190/150‑1 (MQ=255)
                                                                                                                                  cAATCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGgtcgt                <  2:37942/151‑1 (MQ=255)
                                                                                                                                    aTCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTcc              >  1:1341214/1‑151 (MQ=255)
                                                                                                                                     tCTGGTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTcc              >  1:225302/1‑150 (MQ=255)
                                                                                                                                        ggTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCa             <  1:1347772/148‑1 (MQ=255)
                                                                                                                                        ggTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCAtt           >  1:1030305/1‑150 (MQ=255)
                                                                                                                                        ggTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCAtt           <  1:1017853/150‑1 (MQ=255)
                                                                                                                                        ggTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCAtt           <  1:1507519/150‑1 (MQ=255)
                                                                                                                                         gTGCTAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTaa         <  2:1433903/151‑1 (MQ=255)
                                                                                                                                             tAAAGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTc     >  2:1213926/1‑151 (MQ=255)
                                                                                                                                              aaaGAGCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCg    <  2:1215493/151‑1 (MQ=255)
                                                                                                                                                agagCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCt  >  1:1202245/1‑151 (MQ=255)
                                                                                                                                                agagCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCt  <  1:1900687/151‑1 (MQ=255)
                                                                                                                                                agagCATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCt  <  1:930897/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATCGAAGTCGATAACGTGTCATTTGCTTATCGCGATGACAATCTGGTGCTAAAGAACATTAATCTCTCTGTGCCTTCGCGCAATTTTGTGGCGCTGGTCGGGCATACCGGCAGTGGCAAAAGCACCCTCGCCAGTTTATTGATGGGCTATTACCCGCTAACGGAAGGTGAGATTCGCCTTGATGGTCGTCCATTAAGTTCGCT  >  NC_000913/471564‑471858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: