Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,070,398 A→G R186R (CGT→CGC rutE ← putative malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,070,3980AG99.3% 543.1 / NA 148R186R (CGT→CGCrutEputative malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (87/60);  total (88/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

GGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCC  >  NC_000913/1070250‑1070545
                                                                                                                                                    |                                                                                                                                                   
ggTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCg                                                                                                                                                   <  1:19927/151‑1 (MQ=255)
     aCCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCa                                                                                                                                              <  1:2162556/151‑1 (MQ=255)
      cccGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGc                                                                                                                                               <  2:2109197/149‑1 (MQ=255)
      cccGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGc                                                                                                                                               >  1:197301/1‑149 (MQ=255)
      cccGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCa                                                                                                                                              <  2:1426882/150‑1 (MQ=255)
      cccGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAg                                                                                                                                             <  1:1485090/151‑1 (MQ=255)
        cGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGgc                                                                                                                                           <  1:840814/151‑1 (MQ=255)
        cGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGgc                                                                                                                                           <  2:961462/151‑1 (MQ=255)
        cGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGgc                                                                                                                                           <  2:695979/151‑1 (MQ=255)
          gcgcgcCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGgcgc                                                                                                                                         <  1:142298/151‑1 (MQ=255)
              gcCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCAt                                                                                                                                     >  1:989643/1‑151 (MQ=255)
               cccGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATa                                                                                                                                    <  2:2158229/151‑1 (MQ=255)
                 cGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATaaa                                                                                                                                  <  2:1207824/151‑1 (MQ=255)
                 cGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATaaa                                                                                                                                  <  1:211578/151‑1 (MQ=255)
                 cGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATaa                                                                                                                                   >  1:244329/1‑150 (MQ=255)
                  ggCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATaa                                                                                                                                   >  2:740326/1‑149 (MQ=255)
                  ggCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATaa                                                                                                                                   >  1:755321/1‑149 (MQ=255)
                     tGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCtt                                                                                                                              >  1:594803/1‑151 (MQ=255)
                      ggACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCtt                                                                                                                              <  2:557542/150‑1 (MQ=255)
                      ggACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTtg                                                                                                                             >  1:1416754/1‑151 (MQ=255)
                         ccgtccgtGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTtgctg                                                                                                                          >  2:1911435/1‑151 (MQ=255)
                            tccgtGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTgctgct                                                                                                                        >  1:1346762/1‑150 (MQ=255)
                             ccgtGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTa                                                                                                                       <  2:353677/150‑1 (MQ=255)
                                 ggtgATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGc                                                                                                                   <  2:1496751/150‑1 (MQ=255)
                                  gtgATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCa                                                                                                                 <  1:352934/151‑1 (MQ=255)
                                   tgATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCAt                                                                                                                >  1:1446679/1‑151 (MQ=255)
                                    gATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATa                                                                                                               <  1:989396/151‑1 (MQ=255)
                                     ataataTTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAg                                                                                                              <  1:1610955/151‑1 (MQ=255)
                                      taataTTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAg                                                                                                              >  2:1264908/1‑150 (MQ=255)
                                        atatTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCg                                                                                                           <  1:1435723/151‑1 (MQ=255)
                                         tatTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCg                                                                                                           >  1:862617/1‑150 (MQ=255)
                                          atTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGat                                                                                                         <  2:1883723/151‑1 (MQ=255)
                                          atTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGa                                                                                                          <  2:244328/150‑1 (MQ=255)
                                            tGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGatat                                                                                                       >  2:1246771/1‑151 (MQ=255)
                                             gACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGatat                                                                                                       <  1:1285436/150‑1 (MQ=255)
                                             gACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATAtt                                                                                                      >  2:1850165/1‑151 (MQ=255)
                                             gACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATAtt                                                                                                      <  1:133033/151‑1 (MQ=255)
                                              aacaccgcGCCCAAGCAGGACATCGCATCGCGAAAAGTGTGTTGATCGACAATGTTCACAGTTTGCTCCTTACAACAGCCCGCAGACTTATTCAAAGGGCAGACGTGGCAGGCGCGCATAAAAGTTGCTCCGATCCCCCTAGCCCAtttt                                                                                                      >  2:250158/5‑150 (MQ=255)
                                                     cgcCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATcagc                                                                                               >  1:640797/1‑150 (MQ=255)
                                                      gcCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATcagc                                                                                               >  1:1266869/1‑149 (MQ=255)
                                                       cccATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGa                                                                                            <  1:933236/151‑1 (MQ=255)
                                                         cATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGAtt                                                                                          >  1:105644/1‑151 (MQ=255)
                                                          aTGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTg                                                                                         <  1:1741519/151‑1 (MQ=255)
                                                          aTGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTg                                                                                         >  1:451255/1‑151 (MQ=255)
                                                            gCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGc                                                                                        >  2:1412229/1‑150 (MQ=255)
                                                                 aCATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCa                                                                                  <  2:1576870/151‑1 (MQ=255)
                                                                  catcgcatcgCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAg                                                                                 <  2:1857224/151‑1 (MQ=255)
                                                                     cgcatcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCg                                                                               <  1:577837/150‑1 (MQ=255)
                                                                     cgcatcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGt                                                                              >  1:526762/1‑151 (MQ=255)
                                                                      gcatcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGtg                                                                             >  2:1973803/1‑151 (MQ=255)
                                                                        atcgcGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGtgc                                                                            >  2:443137/1‑150 (MQ=255)
                                                                          cgcgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGtgctgc                                                                         >  2:725140/1‑151 (MQ=255)
                                                                           gcgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGcc                                                                        >  1:1821831/1‑151 (MQ=255)
                                                                            cgAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGccc                                                                       >  1:1974788/1‑151 (MQ=255)
                                                                             gAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCg                                                                      >  1:1733486/1‑151 (MQ=255)
                                                                             gAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCg                                                                      >  2:1346676/1‑151 (MQ=255)
                                                                              aaaaGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGt                                                                     >  1:2048528/1‑151 (MQ=255)
                                                                              aaaaGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGt                                                                     >  2:2157683/1‑151 (MQ=255)
                                                                               aaaGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCg                                                                      >  1:1602630/1‑149 (MQ=255)
                                                                                aaGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaa                                                                   <  1:1449397/151‑1 (MQ=255)
                                                                                 aGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaaa                                                                  <  2:680036/151‑1 (MQ=255)
                                                                                 aGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaaa                                                                  <  1:1437832/151‑1 (MQ=255)
                                                                                 aGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaa                                                                   >  1:2070066/1‑150 (MQ=255)
                                                                                 aGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaa                                                                   >  2:1970244/1‑150 (MQ=255)
                                                                                  gTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTaaaa                                                                 <  1:771207/151‑1 (MQ=255)
                                                                                        tgATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGc                                                            <  2:1468439/150‑1 (MQ=255)
                                                                                         gATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgt                                                          >  2:1097213/1‑151 (MQ=255)
                                                                                         gATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcg                                                           <  1:2097095/150‑1 (MQ=255)
                                                                                          aTCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgt                                                          >  2:310051/1‑150 (MQ=255)
                                                                                           tCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgtcg                                                        >  1:1154693/1‑151 (MQ=255)
                                                                                            cGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGcgtcgt                                                       <  1:727984/151‑1 (MQ=255)
                                                                                             gACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCgtcgtc                                                      >  1:1805107/1‑151 (MQ=255)
                                                                                              aCAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTcc                                                     <  2:1630367/151‑1 (MQ=255)
                                                                                              aCAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTcc                                                     >  1:1534652/1‑151 (MQ=255)
                                                                                               cAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTcc                                                     >  2:1460833/1‑150 (MQ=255)
                                                                                               cAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTcc                                                     >  2:1185356/1‑150 (MQ=255)
                                                                                                aaTGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCa                                                    >  2:951171/1‑150 (MQ=255)
                                                                                                 aTGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACg                                                  <  1:1246792/151‑1 (MQ=255)
                                                                                                 aTGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACg                                                  >  1:251654/1‑151 (MQ=255)
                                                                                                         aGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGAc                                           <  1:691772/150‑1 (MQ=255)
                                                                                                         aGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACg                                          >  2:377181/1‑151 (MQ=255)
                                                                                                          gTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACgg                                         <  2:412328/151‑1 (MQ=255)
                                                                                                            ttGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGt                                        <  1:447590/150‑1 (MQ=255)
                                                                                                            ttGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTc                                       >  2:1974277/1‑151 (MQ=255)
                                                                                                             tGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTc                                       <  1:2031618/150‑1 (MQ=255)
                                                                                                             tGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTc                                       <  1:1103473/150‑1 (MQ=255)
                                                                                                               cTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCaa                                     >  1:1133732/1‑150 (MQ=255)
                                                                                                               cTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCaa                                     >  1:1242669/1‑150 (MQ=255)
                                                                                                               cTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAACCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCaa                                     >  2:786298/1‑150 (MQ=255)
                                                                                                                        acaGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACAt                           <  2:1784998/151‑1 (MQ=255)
                                                                                                                          aGCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCg                         >  1:58085/1‑151 (MQ=255)
                                                                                                                           gCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCgg                        >  2:612703/1‑151 (MQ=255)
                                                                                                                           gCCCGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCgg                        >  1:1065792/1‑151 (MQ=255)
                                                                                                                             ccGCAGGCTTCTTCAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCggg                       >  2:655214/1‑150 (MQ=255)
                                                                                                                                        ttcAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGt           >  2:2093809/1‑151 (MQ=255)
                                                                                                                                         tcAAAGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCa             <  1:537765/148‑1 (MQ=255)
                                                                                                                                           aaaGGACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTccc        >  1:38766/1‑151 (MQ=255)
                                                                                                                                              ggACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAgc     <  2:27855/151‑1 (MQ=255)
                                                                                                                                               gACAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAgcg    <  2:1210016/151‑1 (MQ=255)
                                                                                                                                                 cAGGCGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGcc  <  2:1354997/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGTGAACCCGGCGCGCCCGGCTGGACCGTCCGTGGTGATAATATTGACCGCCGCGCCCATGCAGGACATCGCATCGCGAAAAGTTTGTTGATCGACAATGTTCATAGTTTGCTCCTTACAACAGCCCGCAGGCTTCTTCAAAGGACAGACGTGGCAGGCGCGCATAAAGCTTGCTGCTATCGCCATAGCCGATATTAATCAGCAGATTGCTCTTCAGCGTGCTGCCCGTAAAAAAGGCGTCGTCCACGTGTTGACGGTCAAAGCCCGACATCGGGCCGGTATCCAGTCCCAGCGCC  >  NC_000913/1070250‑1070545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: