BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,091,158	T→C	T378A (ACC→GCC)	pgaA ←	biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,091,158	0	T	C	100.0%	515.0 / NA	129	T378A (ACC→GCC)	pgaA	biofilm adhesin polysaccharide PGA secretin; OM porin; poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (69/60); total (69/60)

Only 100 of 129 total aligned reads displayed.

TCTGGCTGTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGTATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGG  >  NC_000913/1091015‑1091305

 ctGGCTGTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGtt                                                                                                                                                <  2:2138690/148‑1 (MQ=255)

   ggCTGTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTgg                                                                                                                                              <  1:1889195/148‑1 (MQ=255)

    gCTGTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGa                                                                                                                                          <  2:1429877/151‑1 (MQ=255)

       gTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACa                                                                                                                                        <  2:1617520/150‑1 (MQ=255)

          aTTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGtt                                                                                                                                     >  1:1191210/1‑150 (MQ=255)

           tttCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGtt                                                                                                                                     >  2:1736743/1‑149 (MQ=255)

            ttCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAgc                                                                                                                                  >  1:870004/1‑151 (MQ=255)

              cAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAgcgc                                                                                                                                <  2:503612/151‑1 (MQ=255)

              cAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAgcgc                                                                                                                                >  2:175535/1‑151 (MQ=255)

              cAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAgcgc                                                                                                                                <  2:2012943/151‑1 (MQ=255)

               aGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAgcgcg                                                                                                                               >  1:1623007/1‑151 (MQ=255)

                 ccTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGcc                                                                                                                             >  2:939245/1‑151 (MQ=255)

                  cTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGccc                                                                                                                            >  2:1074237/1‑151 (MQ=255)

                  cTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGccc                                                                                                                            >  1:1930619/1‑151 (MQ=255)

                  cTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGccc                                                                                                                            >  2:2121685/1‑151 (MQ=255)

                  cTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGccc                                                                                                                            >  1:1192637/1‑151 (MQ=255)

                     aGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCgg                                                                                                                          >  2:1664975/1‑150 (MQ=255)

                      ggAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGAt                                                                                                                        <  2:1302279/151‑1 (MQ=255)

                       gAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATa                                                                                                                       <  1:594882/151‑1 (MQ=255)

                         aGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAAt                                                                                                                     >  2:1199097/1‑151 (MQ=255)

                           aTCATTACTATATTTTGCTACGGTTGCGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGCAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATACttt                                                                                                                   >  2:762800/1‑151 (MQ=255)

                           aTCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACTGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAAttt                                                                                                                   >  1:2142287/1‑151 (MQ=255)

                             cATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTc                                                                                                                 >  2:2079396/1‑151 (MQ=255)

                              aTTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTc                                                                                                                 >  1:1268747/1‑150 (MQ=255)

                                tACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCAct                                                                                                              >  1:461624/1‑151 (MQ=255)

                                 aCTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCActc                                                                                                             <  1:1326693/151‑1 (MQ=255)

                                    atatTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTcc                                                                                                          >  2:1407011/1‑151 (MQ=255)

                                     tatTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCACGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTcc                                                                                                          >  2:348136/1‑150 (MQ=255)

                                      atTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTcc                                                                                                          >  1:464065/1‑149 (MQ=255)

                                        tttGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAgg                                                                                                       <  2:35009/150‑1 (MQ=255)

                                          tGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTgg                                                                                                    >  1:2050259/1‑151 (MQ=255)

                                          tGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTgg                                                                                                    >  1:164309/1‑151 (MQ=255)

                                           gCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTgg                                                                                                    <  1:1598515/150‑1 (MQ=255)

                                           gCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGc                                                                                                   >  2:1536163/1‑151 (MQ=255)

                                               cGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGt                                                                                                >  1:1428539/1‑150 (MQ=255)

                                               cGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTa                                                                                               <  1:750375/151‑1 (MQ=255)

                                                ggTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTa                                                                                               >  1:279118/1‑150 (MQ=255)

                                                    gagagaAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTaaa                                                                                             >  1:1937426/1‑148 (MQ=255)

                                                    gagagaAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTaaa                                                                                             >  1:924408/1‑148 (MQ=255)

                                                         aaaCGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATcc                                                                                     >  2:50449/1‑151 (MQ=255)

                                                         aaaCGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATcc                                                                                     <  1:665501/151‑1 (MQ=255)

                                                          aaCGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCg                                                                                    <  1:175843/151‑1 (MQ=255)

                                                          aaCGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGT‑AAAGAGATCCGc                                                                                   >  2:1442497/1‑151 (MQ=255)

                                                            cGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCa                                                                                  <  2:942210/151‑1 (MQ=255)

                                                             gAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCaa                                                                                 <  2:2149872/151‑1 (MQ=255)

                                                             gAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCa                                                                                  >  1:1968567/1‑150 (MQ=255)

                                                              aaTGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAg                                                                                <  1:1810944/151‑1 (MQ=255)

                                                                  ccccTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGttct                                                                            <  2:331315/151‑1 (MQ=255)

                                                                   cccTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGttctt                                                                           >  2:874427/1‑151 (MQ=255)

                                                                    ccTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGttcttc                                                                          <  1:478541/151‑1 (MQ=255)

                                                                      tGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCAt                                                                        >  1:1752304/1‑151 (MQ=255)

                                                                         aaCCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCgg                                                                     <  1:804946/151‑1 (MQ=255)

                                                                         aaCCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCgg                                                                     >  1:39824/1‑151 (MQ=255)

                                                                                  tCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCg                                                            >  2:741320/1‑151 (MQ=255)

                                                                                   cATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCgg                                                           <  2:2046204/151‑1 (MQ=255)

                                                                                   cATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCgg                                                           >  1:571251/1‑151 (MQ=255)

                                                                                    aTTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCggg                                                          <  2:1485633/151‑1 (MQ=255)

                                                                                      tCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCgggg                                                         <  2:236313/150‑1 (MQ=255)

                                                                                       cGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCGTCGGATAAATCCGGGGCa                                                       <  2:1268726/151‑1 (MQ=255)

                                                                                          gATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATg                                                    <  2:1303289/151‑1 (MQ=255)

                                                                                           aTGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATgg                                                   <  2:821344/151‑1 (MQ=255)

                                                                                                 gTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCt                                             >  1:1289910/1‑151 (MQ=255)

                                                                                                   aGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCtt                                            <  1:1594019/150‑1 (MQ=255)

                                                                                                      cGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTtgtg                                         >  2:1193408/1‑150 (MQ=255)

                                                                                                       gTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGAt                                       >  2:1533343/1‑151 (MQ=255)

                                                                                                       gTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGAt                                       >  2:113029/1‑151 (MQ=255)

                                                                                                         gCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATaa                                     <  2:931639/151‑1 (MQ=255)

                                                                                                         gCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATa                                      <  1:1068513/150‑1 (MQ=255)

                                                                                                         gCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATa                                      <  2:188478/150‑1 (MQ=255)

                                                                                                          cccATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATaaa                                    <  2:223034/151‑1 (MQ=255)

                                                                                                           ccATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATaaaa                                   <  2:1931686/151‑1 (MQ=255)

                                                                                                             aTTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATaaa                                    >  1:310317/1‑148 (MQ=255)

                                                                                                             aTTAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAaga                                 <  2:1375000/151‑1 (MQ=255)

                                                                                                              ttAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAag                                  >  2:751194/1‑149 (MQ=255)

                                                                                                               tAACCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAagag                                <  2:635358/150‑1 (MQ=255)

                                                                                                                 aCCGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTc                             <  2:350795/151‑1 (MQ=255)

                                                                                                                  ccGAAGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCg                            <  1:1480491/151‑1 (MQ=255)

                                                                                                                     aaGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGGCAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTc                         <  2:793435/151‑1 (MQ=255)

                                                                                                                     aaGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTc                         >  2:335663/1‑151 (MQ=255)

                                                                                                                      aGGAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTc                         >  2:879884/1‑150 (MQ=255)

                                                                                                                       ggAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCat                       <  1:465097/151‑1 (MQ=255)

                                                                                                                       ggAAAGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCat                       >  1:1808435/1‑151 (MQ=255)

                                                                                                                         aaaGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCatta                     <  1:1100742/151‑1 (MQ=255)

                                                                                                                         aaaGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCatta                     <  2:189519/151‑1 (MQ=255)

                                                                                                                         aaaGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCatt                      >  1:1806789/1‑150 (MQ=255)

                                                                                                                           aGGCGGCGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCattatt                   <  2:1795116/151‑1 (MQ=255)

                                                                                                                            ggcggcGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTg                  <  2:1039470/151‑1 (MQ=255)

                                                                                                                            ggcggcGAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTg                  <  1:1736774/151‑1 (MQ=255)

                                                                                                                             gcggcgAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGa                 <  2:544350/151‑1 (MQ=255)

                                                                                                                              cggcgAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGAc                >  1:365533/1‑151 (MQ=255)

                                                                                                                              cggcgAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGAc                >  2:508257/1‑151 (MQ=255)

                                                                                                                               ggcgAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATCTTCACTCTCCAGGTGGCTGCAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCCCGGCAATGGTCCCCTTGTGATAAAAGAGCCCGGTCattat                    >  1:855752/1‑146 (MQ=255)

                                                                                                                                gcgAAGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACtg              >  1:1663817/1‑151 (MQ=255)

                                                                                                                                   aaGTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGc           >  2:701944/1‑151 (MQ=255)

                                                                                                                                     gTATTAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCtt         <  2:185735/151‑1 (MQ=255)

                                                                                                                                        ttAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCttttt      >  2:1159406/1‑151 (MQ=255)

                                                                                                                                        ttAATGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCttttt      >  1:1739352/1‑151 (MQ=255)

                                                                                                                                          aaTGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTc    <  2:1989379/151‑1 (MQ=255)

                                                                                                                                           aTGGCATGTTGGGTGATAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCg   <  2:645960/151‑1 (MQ=255)

                                                                                                                                            tGGCATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCgg  >  2:1830655/1‑151 (MQ=255)

TCTGGCTGTCATTTCAGCCTGAGGAAGATCATTACTATATTTTGCTACGGTTGAGAGAAACGAATGCCCCTGTAACCAGGTATCATTCGGGATGCTCGTAGGCGTGCCCATTAACCGAAGGAAAGGCGGCGAAGTATTAATGGTATGTTGGGTGACAGTTAGCGCGCCCGGATAATTTTCACTCTCCAGGTGGCTGTAAAAGAGATCCGCAAGTTCTTCATCGGATAAATCCGGGGCAATGGTCTCCTTGTGATAAAAGAGCTCGGTCATTATTGACTGTGCTTTTTTCGG  >  NC_000913/1091015‑1091305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`