Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,274,435 A→G T218A (ACA→GCA)  ychO → putative invasin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,274,4350AG100.0% 550.8 / NA 141T218A (ACA→GCA) ychOputative invasin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (89/52);  total (89/52)

Only 100 of 142 total aligned reads displayed.

GCAACTGGCTGGTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTAT  >  NC_000913/1274289‑1274581
                                                                                                                                                  |                                                                                                                                                  
gCAACTGGCTGGTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGc                                                                                                                                                <  2:567518/151‑1 (MQ=255)
gCAACTGGCTGGTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGc                                                                                                                                                >  1:1618097/1‑151 (MQ=255)
    ctggctggTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACg                                                                                                                                            >  2:519586/1‑151 (MQ=255)
     tggctggTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGc                                                                                                                                           >  1:89113/1‑151 (MQ=255)
     tggctggTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGc                                                                                                                                           >  1:623376/1‑151 (MQ=255)
     tggctggTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGc                                                                                                                                           >  1:131990/1‑151 (MQ=255)
      ggctggTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGc                                                                                                                                           >  1:690929/1‑150 (MQ=255)
         tggtggGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGTGCGAATATTTGCGATTATCCGCAAACTTTTATCAGACGTTTGCTGCATGGCATGAACAGGCAGCCACGCAg                                                                                                                                         >  2:1008979/1‑149 (MQ=255)
         tggtggGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAgg                                                                                                                                        >  1:1071855/1‑150 (MQ=255)
          ggtggGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGaa                                                                                                                                      <  2:1109265/151‑1 (MQ=255)
            tggGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGaac                                                                                                                                     >  2:2018641/1‑150 (MQ=255)
             gggTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGaaca                                                                                                                                    >  2:432962/1‑150 (MQ=255)
               gTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACg                                                                                                                                 >  1:1896983/1‑151 (MQ=255)
               gTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACg                                                                                                                                 <  2:1784593/151‑1 (MQ=255)
                ttATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACgg                                                                                                                                >  1:50150/1‑151 (MQ=255)
                 tataACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACgg                                                                                                                                <  2:673612/150‑1 (MQ=255)
                 tataACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACgg                                                                                                                                >  2:29135/1‑150 (MQ=255)
                  ataACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGAt                                                                                                                              >  1:54889/1‑151 (MQ=255)
                  ataACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGAt                                                                                                                              <  1:1206441/151‑1 (MQ=255)
                    aaCACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATgg                                                                                                                            >  1:1914473/1‑151 (MQ=255)
                     acacTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgc                                                                                                                           >  2:2119890/1‑151 (MQ=255)
                      cacTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgcg                                                                                                                          <  1:340105/151‑1 (MQ=255)
                       acTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgcgc                                                                                                                         >  2:363663/1‑151 (MQ=255)
                        cTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgcgcg                                                                                                                        >  1:573996/1‑151 (MQ=255)
                         tttttATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgcgcgc                                                                                                                       >  2:1182218/1‑151 (MQ=255)
                         tttttATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGgcgcgc                                                                                                                       >  2:2097346/1‑151 (MQ=255)
                          ttttATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCGAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGcgcgcg                                                                                                                      >  2:455385/1‑151 (MQ=255)
                            ttATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCggg                                                                                                                    >  2:53838/1‑151 (MQ=255)
                             tATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGt                                                                                                                   >  1:535361/1‑151 (MQ=255)
                              aTGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGt                                                                                                                   >  2:1787738/1‑150 (MQ=255)
                              aTGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGt                                                                                                                   <  2:2149772/150‑1 (MQ=255)
                                gACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAGTATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACg                                                                                                                >  2:94293/1‑151 (MQ=255)
                                gACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACg                                                                                                                <  1:1423827/151‑1 (MQ=255)
                                 aCAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACg                                                                                                                >  2:1289844/1‑150 (MQ=255)
                                   aaCTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGAcc                                                                                                             >  1:1526987/1‑151 (MQ=255)
                                       tGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGAc                                                                                                         >  2:1301567/1‑151 (MQ=255)
                                         cTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGAc                                                                                                         >  1:1111164/1‑149 (MQ=255)
                                          tGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGc                                                                                                      >  2:334692/1‑151 (MQ=255)
                                                aaaaTCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGAt                                                                                                <  1:2043026/151‑1 (MQ=255)
                                                  aaTCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATgc                                                                                              <  1:844526/151‑1 (MQ=255)
                                                  aaTCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATgc                                                                                              <  1:905272/151‑1 (MQ=255)
                                                  aaTCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATgc                                                                                              >  2:500172/1‑151 (MQ=255)
                                                        cAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATg                                                                                         <  1:2106856/150‑1 (MQ=255)
                                                        cAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGc                                                                                        >  1:2125092/1‑151 (MQ=255)
                                                            gagcgGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGtt                                                                                    <  1:792490/151‑1 (MQ=255)
                                                             agcgGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTc                                                                                   >  1:283461/1‑151 (MQ=255)
                                                                  gCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATc                                                                               >  1:771985/1‑150 (MQ=255)
                                                                      tGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAacac                                                                          >  2:1954985/1‑151 (MQ=255)
                                                                          gCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTc                                                                       >  1:298714/1‑150 (MQ=255)
                                                                              aaGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATAc                                                                  >  2:627903/1‑151 (MQ=255)
                                                                              aaGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATAc                                                                  >  2:1200600/1‑151 (MQ=255)
                                                                               aGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATAcc                                                                 >  2:1639856/1‑151 (MQ=255)
                                                                                 cGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATAcc                                                                 >  2:1453932/1‑149 (MQ=255)
                                                                                  gTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgt                                                              >  1:1364999/1‑151 (MQ=255)
                                                                                  gTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAgt                                                              >  2:1278534/1‑151 (MQ=255)
                                                                                     gggCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTc                                                           <  2:77336/151‑1 (MQ=255)
                                                                                      ggCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCa                                                          <  1:712566/151‑1 (MQ=255)
                                                                                       gCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTc                                                           >  1:1035371/1‑149 (MQ=255)
                                                                                        cGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGc                                                        >  1:177634/1‑151 (MQ=255)
                                                                                         gAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGcc                                                       <  2:236132/151‑1 (MQ=255)
                                                                                         gAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGcc                                                       >  1:541811/1‑151 (MQ=255)
                                                                                          aaTATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCt                                                      >  1:1085902/1‑151 (MQ=255)
                                                                                           atatTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCt                                                      >  1:1140784/1‑150 (MQ=255)
                                                                                           atatTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTa                                                     <  1:172849/151‑1 (MQ=255)
                                                                                             atTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCt                                                      >  1:1850955/1‑148 (MQ=255)
                                                                                              tttGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGa                                                   >  2:1934855/1‑150 (MQ=255)
                                                                                              tttGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGa                                                   >  1:1840786/1‑150 (MQ=255)
                                                                                              tttGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGa                                                   >  2:1138828/1‑150 (MQ=255)
                                                                                              tttGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGa                                                   >  1:1007596/1‑150 (MQ=255)
                                                                                                  cGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGt                                              >  1:1836666/1‑151 (MQ=255)
                                                                                                   gATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGt                                              >  1:2114210/1‑150 (MQ=255)
                                                                                                    aTTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAt                                            <  1:1527138/151‑1 (MQ=255)
                                                                                                     ttATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAtt                                           >  1:1378799/1‑151 (MQ=255)
                                                                                                       aTCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTAtttt                                         <  2:416771/151‑1 (MQ=255)
                                                                                                           gCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTg                                     <  1:325970/151‑1 (MQ=255)
                                                                                                           gCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTg                                     <  1:105712/151‑1 (MQ=255)
                                                                                                            cAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTg                                     >  2:1149625/1‑150 (MQ=255)
                                                                                                             aaaCTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACTCCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGAt                                   <  1:1619897/151‑1 (MQ=255)
                                                                                                              aaCTTTTATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATc                                  >  2:1350724/1‑151 (MQ=255)
                                                                                                                  tttATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCgtg                               <  2:298752/150‑1 (MQ=255)
                                                                                                                  tttATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCgtg                               <  2:1392256/150‑1 (MQ=255)
                                                                                                                   ttATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCgtgt                              <  1:11200/150‑1 (MQ=255)
                                                                                                                    tATCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGtt                             <  2:241345/150‑1 (MQ=255)
                                                                                                                      tCAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGa                           <  2:1930969/150‑1 (MQ=255)
                                                                                                                       cAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGAtt                         >  2:848648/1‑151 (MQ=255)
                                                                                                                       cAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGAtt                         <  2:275904/151‑1 (MQ=255)
                                                                                                                       cAGCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGAt                          <  1:1945337/150‑1 (MQ=255)
                                                                                                                         gCCGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTg                       >  2:2032727/1‑151 (MQ=255)
                                                                                                                          ccGTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGt                      >  2:926501/1‑151 (MQ=255)
                                                                                                                            gTTTGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGttt                    <  1:160366/151‑1 (MQ=255)
                                                                                                                              ttGCTGCATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTa                   <  1:1248669/150‑1 (MQ=255)
                                                                                                                                gctgcATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAct                <  1:432140/151‑1 (MQ=255)
                                                                                                                                gctgcATGGCATGAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAAct                >  1:933920/1‑151 (MQ=255)
                                                                                                                                    catggcatgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTgg            >  1:849131/1‑151 (MQ=255)
                                                                                                                                     atggcatgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGt           >  1:664558/1‑151 (MQ=255)
                                                                                                                                       ggcatgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTa          <  1:1578405/150‑1 (MQ=255)
                                                                                                                                       ggcatgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTAc         <  1:1597175/151‑1 (MQ=255)
                                                                                                                                         catgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACgg       <  1:1298287/151‑1 (MQ=255)
                                                                                                                                          atgAACAGGCAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACggg      >  2:2100317/1‑151 (MQ=255)
                                                                                                                                              acaggcagCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTAt  >  2:641202/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCAACTGGCTGGTGGGTTATAACACTTTTTATGACAACTTGCTGGACGAAAATCTTCAGCGAGCGGGCTTTGGTGCCGAAGCGTGGGGCGAATATTTGCGATTATCGGCAAACTTTTATCAGCCGTTTGCTGCATGGCATGAACAGACAGCCACGCAGGAACAACGGATGGCGCGCGGGTACGACCTGACAGCTCGGATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTAT  >  NC_000913/1274289‑1274581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: