Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,941,604 G→A A11V (GCG→GTG)  mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,941,6040GA99.3% 560.4 / NA 143A11V (GCG→GTG) mepMmurein DD‑endopeptidase, space‑maker hydrolase, septation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (70/72);  minor base T (0/1);  total (70/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

ACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCC  >  NC_000913/1941456‑1941750
                                                                                                                                                    |                                                                                                                                                  
acgaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACAcc                                                                                                                                                  >  1:181877/1‑151 (MQ=255)
 cgaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCa                                                                                                                                                 <  1:10/151‑1 (MQ=255)
 cgaaTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCa                                                                                                                                                 <  1:644100/151‑1 (MQ=255)
   aaTGTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAgg                                                                                                                                               <  2:1787799/151‑1 (MQ=255)
    aTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAggg                                                                                                                                              >  2:479251/1‑151 (MQ=255)
    aTTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAggg                                                                                                                                              >  1:326041/1‑151 (MQ=255)
              gTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACaga                                                                                                                                       <  2:1863485/148‑1 (MQ=255)
                tCCAGCTCAATTGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCgg                                                                                                                                   <  2:1583650/150‑1 (MQ=255)
                tCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  >  2:347433/1‑151 (MQ=255)
                tCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  >  1:706179/1‑151 (MQ=255)
                 ccAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCggg                                                                                                                                  <  2:967659/150‑1 (MQ=255)
                   aGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCt                                                                                                                                >  1:1757724/1‑150 (MQ=255)
                   aGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTa                                                                                                                               <  1:542449/151‑1 (MQ=255)
                   aGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTa                                                                                                                               <  1:1643663/151‑1 (MQ=255)
                    gCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTAt                                                                                                                              <  1:1011698/151‑1 (MQ=255)
                    gCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTAt                                                                                                                              <  1:590023/151‑1 (MQ=255)
                      tCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCt                                                                                                                            >  1:1837706/1‑151 (MQ=255)
                       cAATGGTTTTGACAATTTGCGTGGCATCACTGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAATAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAACCTCCAGGGCGACAGAGCGGGCTATCt                                                                                                                            <  2:1078200/150‑1 (MQ=255)
                        aaTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGt                                                                                                                          >  2:1834052/1‑151 (MQ=255)
                         aTGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGtt                                                                                                                         <  1:1031689/151‑1 (MQ=255)
                           ggTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGc                                                                                                                       <  2:1498632/151‑1 (MQ=255)
                             ttttGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCAc                                                                                                                     >  2:542482/1‑151 (MQ=255)
                              tttGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCAc                                                                                                                     <  1:1015068/150‑1 (MQ=255)
                                  aCAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAAt                                                                                                                <  1:2023887/151‑1 (MQ=255)
                                    aaTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                               >  1:1095448/1‑150 (MQ=255)
                                    aaTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATc                                                                                                               >  1:721740/1‑150 (MQ=255)
                                    aaTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCa                                                                                                              <  2:1426476/151‑1 (MQ=255)
                                    aaTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCa                                                                                                              <  1:320272/151‑1 (MQ=255)
                                     aTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCAc                                                                                                             >  1:903081/1‑151 (MQ=255)
                                     aTTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGGAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCAc                                                                                                             >  2:1484995/1‑151 (MQ=255)
                                         gCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTcc                                                                                                         >  1:1121529/1‑151 (MQ=255)
                                           gTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTc                                                                                                       >  1:423273/1‑151 (MQ=255)
                                              gCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCAtt                                                                                                    <  1:1717358/151‑1 (MQ=255)
                                               cATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCAtt                                                                                                    >  1:1414710/1‑150 (MQ=255)
                                               cATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTa                                                                                                   <  1:96848/151‑1 (MQ=255)
                                                aTCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTaa                                                                                                  <  2:1205567/151‑1 (MQ=255)
                                                aTCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTa                                                                                                   >  1:36506/1‑150 (MQ=255)
                                                 tCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAt                                                                                                 >  1:423845/1‑151 (MQ=255)
                                                   aCGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGTACGTAATCACTTCCTCATTAAtct                                                                                               <  1:1605375/151‑1 (MQ=255)
                                                   aCGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAAtct                                                                                               <  2:221494/151‑1 (MQ=255)
                                                        gATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCttt                                                                                          <  2:2114319/151‑1 (MQ=255)
                                                         aTAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTc                                                                                         <  1:1594911/151‑1 (MQ=255)
                                                          tAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTc                                                                                         >  2:251042/1‑150 (MQ=255)
                                                             aCATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                                     >  2:1754598/1‑151 (MQ=255)
                                                               aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCt                                                                                            <  2:1413097/142‑1 (MQ=255)
                                                               aTAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGc                                                                                     >  2:1043690/1‑149 (MQ=255)
                                                                tAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGc                                                                                  >  2:771546/1‑151 (MQ=255)
                                                                  aGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTc                                                                                <  2:1032957/151‑1 (MQ=255)
                                                                  aGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTc                                                                                >  2:1363476/1‑151 (MQ=255)
                                                                   ggCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCg                                                                               <  1:1915719/151‑1 (MQ=255)
                                                                       gccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCGGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCAt                                                                            >  1:1445648/1‑150 (MQ=255)
                                                                       gccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATa                                                                           <  1:1994868/151‑1 (MQ=255)
                                                                        ccAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATAc                                                                          >  2:215753/1‑151 (MQ=255)
                                                                          aGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTg                                                                        >  1:1891589/1‑151 (MQ=255)
                                                                           gACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTgg                                                                       <  2:18973/151‑1 (MQ=255)
                                                                             cAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGtt                                                                     >  1:463344/1‑151 (MQ=255)
                                                                             cAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGtt                                                                     <  1:254979/151‑1 (MQ=255)
                                                                             cAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGtt                                                                     >  2:1299581/1‑151 (MQ=255)
                                                                               gCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTgg                                                                   <  1:828379/151‑1 (MQ=255)
                                                                                cGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGc                                                                  >  2:1536904/1‑151 (MQ=255)
                                                                                 gACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCt                                                                 <  2:1752623/151‑1 (MQ=255)
                                                                                 gACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCt                                                                 >  1:2165195/1‑151 (MQ=255)
                                                                                  aCGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTa                                                                <  2:619495/151‑1 (MQ=255)
                                                                                      ccAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTg                                                            <  1:20081/151‑1 (MQ=255)
                                                                                       cAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTg                                                            >  1:1340146/1‑150 (MQ=255)
                                                                                         gagTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACt                                                         >  1:1887250/1‑151 (MQ=255)
                                                                                           gTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCa                                                       <  1:1890451/151‑1 (MQ=255)
                                                                                              aGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGa                                                    <  1:1556794/151‑1 (MQ=255)
                                                                                               gAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGa                                                    >  2:829857/1‑150 (MQ=255)
                                                                                                    gTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTg                                              <  2:896142/151‑1 (MQ=255)
                                                                                                    gTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTg                                              >  2:282844/1‑151 (MQ=255)
                                                                                                      gAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGaa                                            <  1:1904184/151‑1 (MQ=255)
                                                                                                       aGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAAt                                           <  2:462736/151‑1 (MQ=255)
                                                                                                          gACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGc                                        >  1:1595238/1‑151 (MQ=255)
                                                                                                          gACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGc                                        >  1:1203124/1‑151 (MQ=255)
                                                                                                           aCCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCt                                       <  2:966342/151‑1 (MQ=255)
                                                                                                           aCCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCt                                       >  2:2050737/1‑151 (MQ=255)
                                                                                                             cccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTg                                     <  1:1054025/151‑1 (MQ=255)
                                                                                                              ccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGGATAGCTTGt                                    <  1:811974/151‑1 (MQ=255)
                                                                                                              ccAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGt                                    >  1:1146480/1‑151 (MQ=255)
                                                                                                               cAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtt                                   <  1:262723/151‑1 (MQ=255)
                                                                                                                 aCATAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGtttt                                 <  2:1990971/151‑1 (MQ=255)
                                                                                                                    tAACGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAcc                              >  2:1804449/1‑151 (MQ=255)
                                                                                                                      aCGCGGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTAccc                             >  2:1318833/1‑150 (MQ=255)
                                                                                                                        gcgGTGTGGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGt                          >  1:1780323/1‑151 (MQ=255)
                                                                                                                             gtgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGat                     <  1:1651657/151‑1 (MQ=255)
                                                                                                                             gtgGTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGa                      <  1:1099358/150‑1 (MQ=255)
                                                                                                                               ggTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGatat                   >  2:171239/1‑151 (MQ=255)
                                                                                                                               ggTCGCGGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGata                    <  1:190762/150‑1 (MQ=255)
                                                                                                                                  cgcgGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCg                <  2:348091/151‑1 (MQ=255)
                                                                                                                                   gcgGTAAATTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGt               >  1:896467/1‑151 (MQ=255)
                                                                                                                                        aaaTTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTcccc           >  1:630087/1‑150 (MQ=255)
                                                                                                                                        aaaTTATTAAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTcccc           >  1:1690316/1‑150 (MQ=255)
                                                                                                                                           ttattaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAggg       >  1:1913143/1‑151 (MQ=255)
                                                                                                                                            tattaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAgggg      >  2:1367331/1‑151 (MQ=255)
                                                                                                                                            tattaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAggg       <  1:1299602/150‑1 (MQ=255)
                                                                                                                                             attaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAgggg      <  1:1858242/150‑1 (MQ=255)
                                                                                                                                             attaAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAgggg      <  2:1092440/150‑1 (MQ=255)
                                                                                                                                               taAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGAt    <  1:1619676/150‑1 (MQ=255)
                                                                                                                                               taAACACCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATc   >  1:240220/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACGAATTTCGTTCTGTTCCAGCTCAATGGTTTTGACAATTGGCGTGGCATCACGGTGATAAACATAAGGCCGCCAGACAGCGACGGCCAGAGTAAGAACGGTGAGCGACCCCAACATAACGCGGTGTGGTCGCGGTAAATTATTAAACGCCAGGGCGACAGAGCGGGCTATCTGTTGCACGTAATCACTTCCTCATTAATCTCCTTTCAGGCAGCTCGCATACTGGTTGGCTAATTGACTCAGGAATTCTGAATAGCTTGTTTTACCCAGTTTGATATTCGTCCCCAGGGGATCC  >  NC_000913/1941456‑1941750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: