Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,049,656 T→C A1573A (GCT→GCC yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,049,6560TC99.2% 488.9 / NA 128A1573A (GCT→GCCyeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (61/66);  total (61/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 129 total aligned reads displayed.

TCACGCTGAAAGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATAT  >  NC_000913/2049511‑2049802
                                                                                                                                                 |                                                                                                                                                  
tCACGCTGAAAGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCcaata                                                                                                                                                >  1:1752792/1‑150 (MQ=255)
   cGCTGAAAGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGt                                                                                                                                            <  1:1782643/151‑1 (MQ=255)
   cGCTGAAAGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGt                                                                                                                                            <  2:2042029/151‑1 (MQ=255)
        aaaGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCggg                                                                                                                                        >  2:100220/1‑150 (MQ=255)
         aaGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCggg                                                                                                                                        >  2:1152289/1‑149 (MQ=255)
         aaGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGAt                                                                                                                                      >  1:263048/1‑151 (MQ=255)
         aaGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGAt                                                                                                                                      <  2:628244/151‑1 (MQ=255)
          aGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGGGGGGGCAGGTGGTGGTGAACTTTATTGCGGATACGCTCACTTCGCAGGGTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATg                                                                                                                                     >  2:842682/1‑151 (MQ=255)
          aGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATg                                                                                                                                     <  1:749054/151‑1 (MQ=255)
            gTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGAc                                                                                                                                   <  2:1186270/151‑1 (MQ=255)
              aCAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGAcc                                                                                                                                  >  1:1607532/1‑150 (MQ=255)
              aCAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCa                                                                                                                                 <  1:2083926/151‑1 (MQ=255)
                  aaGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCt                                                                                                                             <  2:1990873/151‑1 (MQ=255)
                   aGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTg                                                                                                                            >  2:2136738/1‑151 (MQ=255)
                    gCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGc                                                                                                                           <  2:742608/151‑1 (MQ=255)
                    gCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCCCACTGCGCAGGGTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTg                                                                                                                            >  2:1941237/1‑150 (MQ=255)
                     cAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGc                                                                                                                           >  1:1086554/1‑150 (MQ=255)
                     cAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCa                                                                                                                          <  1:1647157/151‑1 (MQ=255)
                      aGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAg                                                                                                                         <  1:1941972/151‑1 (MQ=255)
                        gcgcTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGc                                                                                                                       <  2:733558/151‑1 (MQ=255)
                           cTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAAc                                                                                                                    >  2:1748436/1‑151 (MQ=255)
                            tCATACTGTTACAGCATCGATGACTGGCGGTTAGGGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTTACTGCGCAGGTTAATTTTAACGTTTCCGAGGACAATTTTATCGCCAATAACGTCGGGATGTCCCGGCTGCAGGCAAc                                                                                                                    >  1:1029653/1‑150 (MQ=255)
                             cATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAAc                                                                                                                    <  1:1238937/149‑1 (MQ=255)
                             cATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAAc                                                                                                                    >  1:1075351/1‑149 (MQ=255)
                                   gTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACt                                                                                                             <  2:1990104/150‑1 (MQ=255)
                                   gTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACt                                                                                                             <  1:1496396/150‑1 (MQ=255)
                                     tACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGa                                                                                                           <  1:386194/150‑1 (MQ=255)
                                     tACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGa                                                                                                           <  1:2013962/150‑1 (MQ=255)
                                      aCAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATg                                                                                                         >  1:97172/1‑151 (MQ=255)
                                      aCAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATg                                                                                                         <  1:1326411/151‑1 (MQ=255)
                                      aCAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGGGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTg                                                                                                            >  2:1377496/1‑148 (MQ=255)
                                       cAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATgg                                                                                                        <  1:427766/151‑1 (MQ=255)
                                         gCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGa                                                                                                       >  2:1069089/1‑150 (MQ=255)
                                          cATCGATGACTGGCGGTAAGAGTGAGCAGATGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGAACAGGCTGCAGGCAACAGTGACTGATGGaa                                                                                                      >  2:772337/1‑150 (MQ=255)
                                           aTCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAAc                                                                                                    >  1:1006415/1‑151 (MQ=255)
                                             cGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACgg                                                                                                  >  1:1045021/1‑151 (MQ=255)
                                              gATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGc                                                                                                 <  2:1890866/151‑1 (MQ=255)
                                              gATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGc                                                                                                 <  2:1360771/151‑1 (MQ=255)
                                                tGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCa                                                                                                >  1:237192/1‑150 (MQ=255)
                                                tGACTGGCGGTAAGAGTGAGCAGTTGGTGGGGGACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCa                                                                                                >  2:778144/1‑150 (MQ=255)
                                                 gACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCaa                                                                                               <  1:1089271/150‑1 (MQ=255)
                                                    tGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCg                                                                                           >  1:34227/1‑151 (MQ=255)
                                                      gCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGtt                                                                                         <  1:53271/151‑1 (MQ=255)
                                                        ggTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGtt                                                                                         >  1:514409/1‑149 (MQ=255)
                                                         gTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGc                                                                                      <  1:623191/151‑1 (MQ=255)
                                                           aaGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGcc                                                                                     >  1:398876/1‑150 (MQ=255)
                                                           aaGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGcc                                                                                     <  2:1572338/150‑1 (MQ=255)
                                                           aaGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGcc                                                                                     <  1:395944/150‑1 (MQ=255)
                                                           aaGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCa                                                                                    <  1:524416/151‑1 (MQ=255)
                                                            agagTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCaa                                                                                   <  1:1685105/151‑1 (MQ=255)
                                                             gagTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAAt                                                                                  <  1:247695/151‑1 (MQ=255)
                                                             gagTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAAt                                                                                  <  1:1807627/151‑1 (MQ=255)
                                                               gTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGa                                                                                <  1:167511/151‑1 (MQ=255)
                                                               gTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGa                                                                                <  1:82968/151‑1 (MQ=255)
                                                                   gCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGGCCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCg                                                                            <  1:1716256/151‑1 (MQ=255)
                                                                    cAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCgg                                                                           >  1:1953127/1‑151 (MQ=255)
                                                                        tggtggTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGAc                                                                       >  1:386614/1‑151 (MQ=255)
                                                                           tggtgAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACAtt                                                                    >  2:1131575/1‑151 (MQ=255)
                                                                            ggtgAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTc                                                                   >  1:1487124/1‑151 (MQ=255)
                                                                               gAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACg                                                                <  1:812768/151‑1 (MQ=255)
                                                                                 aCTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCt                                                              >  1:99775/1‑151 (MQ=255)
                                                                                  cTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCt                                                              >  1:1981790/1‑150 (MQ=255)
                                                                                    ttATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTa                                                             <  2:1993359/149‑1 (MQ=255)
                                                                                        tGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGc                                                        <  1:2027796/150‑1 (MQ=255)
                                                                                            gATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtg                                                   <  1:1537063/151‑1 (MQ=255)
                                                                                            gATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtg                                                   <  2:1940253/151‑1 (MQ=255)
                                                                                               aCGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtgtg                                                 >  1:569635/1‑150 (MQ=255)
                                                                                               aCGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGAtgtg                                                 >  2:1227153/1‑150 (MQ=255)
                                                                                                cGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAg                                               <  1:1238425/151‑1 (MQ=255)
                                                                                                 gCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAg                                               >  2:1821561/1‑150 (MQ=255)
                                                                                                  cTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAgcg                                             >  1:1576541/1‑151 (MQ=255)
                                                                                                     aCTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCaa                                          <  2:689974/151‑1 (MQ=255)
                                                                                                      cTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCa                                           >  1:1684100/1‑149 (MQ=255)
                                                                                                       tGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAg                                         >  2:1040775/1‑150 (MQ=255)
                                                                                                       tGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGc                                        >  1:1648493/1‑151 (MQ=255)
                                                                                                        gcgcAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAg                                         <  1:1144103/149‑1 (MQ=255)
                                                                                                           cAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTa                                    <  1:847615/151‑1 (MQ=255)
                                                                                                           cAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTa                                    <  2:748313/151‑1 (MQ=255)
                                                                                                            aGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTAc                                   >  2:1165138/1‑151 (MQ=255)
                                                                                                              gTTAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTAct                                  >  2:6625/1‑150 (MQ=255)
                                                                                                                tAATCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACtctc                               >  2:1583569/1‑151 (MQ=255)
                                                                                                                 aaTCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACtctc                               <  2:314268/150‑1 (MQ=255)
                                                                                                                 aaTCTTAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCg                              >  2:243134/1‑151 (MQ=255)
                                                                                                                      tAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAAGAGTGACTGATAGAATCGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCgg                             <  2:1597093/147‑1 (MQ=255)
                                                                                                                      tAACGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCgg                             <  1:772217/147‑1 (MQ=255)
                                                                                                                       aaCGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGAc                           >  2:165730/1‑148 (MQ=255)
                                                                                                                       aaCGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACa                          >  1:392884/1‑149 (MQ=255)
                                                                                                                       aaCGTTACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAg                        <  1:473781/151‑1 (MQ=255)
                                                                                                                            tACCGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCgg                    >  1:768440/1‑150 (MQ=255)
                                                                                                                               cGAGGACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGGTcc                >  1:55299/1‑151 (MQ=255)
                                                                                                                                  ggACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGcc             <  1:122835/151‑1 (MQ=255)
                                                                                                                                  ggACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGcc             <  1:2040219/151‑1 (MQ=255)
                                                                                                                                   gACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGcc             >  2:176480/1‑150 (MQ=255)
                                                                                                                                   gACAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCa            <  1:1194136/151‑1 (MQ=255)
                                                                                                                                    aCAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCa            >  2:1604090/1‑150 (MQ=255)
                                                                                                                                     cAATTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGGTCCGCCAtt          >  1:100253/1‑151 (MQ=255)
                                                                                                                                       aTTTTATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCAtt          >  2:646973/1‑149 (MQ=255)
                                                                                                                                         tttATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTa         >  2:1407881/1‑148 (MQ=255)
                                                                                                                                           tATCGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGa     <  2:1135358/150‑1 (MQ=255)
                                                                                                                                              cGCCAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGatat  <  1:121149/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCACGCTGAAAGGTACAAAAGCAGGCGCTCATACTGTTACAGCATCGATGACTGGCGGTAAGAGTGAGCAGTTGGTGGTGAACTTTATTGCGGATACGCTCACTGCGCAGGTTAATCTTAACGTTACCGAGGACAATTTTATCGCTAATAACGTCGGGATGACCAGGCTGCAGGCAACAGTGACTGATGGAAACGGCAACCCGTTAGCCAATGAGGCGGTGACATTCACGCTACCGGCAGATGTGAGCGCAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATAT  >  NC_000913/2049511‑2049802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: