Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,130,545 G→A R112C (CGC→TGC)  wcaD ← putative colanic acid polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,130,5450GA100.0% 574.2 / NA 142R112C (CGC→TGC) wcaDputative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (64/78);  total (64/78)

Only 100 of 142 total aligned reads displayed.

TCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGC  >  NC_000913/2130398‑2130692
                                                                                                                                                   |                                                                                                                                                   
tCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAAt                                                                                                                                                   <  1:587526/150‑1 (MQ=255)
     acaTAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTtgt                                                                                                                                             <  1:489949/151‑1 (MQ=255)
      caTAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTtgtg                                                                                                                                            <  2:1220890/151‑1 (MQ=255)
          gCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGc                                                                                                                                        <  1:1104984/151‑1 (MQ=255)
          gCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGc                                                                                                                                        <  1:1459753/151‑1 (MQ=255)
            tGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCg                                                                                                                                       >  2:239038/1‑150 (MQ=255)
            tGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCg                                                                                                                                       >  2:796265/1‑150 (MQ=255)
              ttGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGaa                                                                                                                                     >  2:958748/1‑150 (MQ=255)
               tGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAAt                                                                                                                                   >  1:1572453/1‑151 (MQ=255)
                gCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAAtt                                                                                                                                  <  2:2153916/151‑1 (MQ=255)
                  tGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGt                                                                                                                                >  2:151002/1‑151 (MQ=255)
                  tGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGt                                                                                                                                >  1:1720454/1‑151 (MQ=255)
                   gTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtt                                                                                                                               <  2:1378208/151‑1 (MQ=255)
                   gTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGt                                                                                                                                <  1:1058589/150‑1 (MQ=255)
                      aaTCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGtttt                                                                                                                             >  1:2019752/1‑150 (MQ=255)
                         cAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTaaa                                                                                                                          <  1:1113694/150‑1 (MQ=255)
                          aGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAAc                                                                                                                        <  1:1644390/151‑1 (MQ=255)
                          aGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAAc                                                                                                                        <  2:314369/151‑1 (MQ=255)
                           gATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACg                                                                                                                       >  2:95641/1‑151 (MQ=255)
                           gATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACg                                                                                                                       <  2:847552/151‑1 (MQ=255)
                             tatTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGa                                                                                                                      >  2:1888710/1‑150 (MQ=255)
                              atTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGaa                                                                                                                     <  1:443823/150‑1 (MQ=255)
                              atTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAAt                                                                                                                    >  1:2148576/1‑151 (MQ=255)
                              atTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAAt                                                                                                                    >  1:83364/1‑151 (MQ=255)
                                  cGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTc                                                                                                                >  2:206065/1‑151 (MQ=255)
                                  cGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTc                                                                                                                >  1:481370/1‑151 (MQ=255)
                                     aaTCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCcac                                                                                                             <  2:1452170/151‑1 (MQ=255)
                                     aaTCGACCCCCAAATACTGGAGGCCCCGGGGAAGATAATTTGGGACATCTCCCCCGCCGCCAGCGGCACCCCCCGCCCGACCACCAGATAAAAAAAACGGAATAACCTGCAATGGGTGTGTGGCGAAATTGTTTTAAAACGAATACTCcac                                                                                                             <  2:1807194/151‑1 (MQ=255)
                                        cGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACAcc                                                                                                          >  2:1825035/1‑151 (MQ=255)
                                          aCTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCAt                                                                                                        <  1:1300721/151‑1 (MQ=255)
                                             ccATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                                                                                                     >  1:39489/1‑151 (MQ=255)
                                             ccATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                                                                                                     >  1:480296/1‑151 (MQ=255)
                                             ccATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGcc                                                                                                     <  2:1459286/151‑1 (MQ=255)
                                              cATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCa                                                                                                    <  2:1402510/151‑1 (MQ=255)
                                                taataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaat                                                                                                  >  1:152282/1‑151 (MQ=255)
                                                 aataCTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCaat                                                                                                  <  2:1699050/150‑1 (MQ=255)
                                                      tgctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGAt                                                                                            >  1:1009215/1‑151 (MQ=255)
                                                      tgctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGAt                                                                                            >  1:1157431/1‑151 (MQ=255)
                                                       gctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                           >  2:1782241/1‑151 (MQ=255)
                                                        ctgcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATc                                                                                           >  2:700691/1‑150 (MQ=255)
                                                          gcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATcac                                                                                         <  1:79645/150‑1 (MQ=255)
                                                          gcCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATca                                                                                          <  2:101620/149‑1 (MQ=255)
                                                           ccACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATcaca                                                                                        >  1:146572/1‑150 (MQ=255)
                                                            cACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAat                                                                                      >  2:715865/1‑151 (MQ=255)
                                                            cACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAat                                                                                      <  1:1671750/151‑1 (MQ=255)
                                                              ccGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAatat                                                                                    >  1:1779940/1‑151 (MQ=255)
                                                                ggTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAg                                                                                  <  2:1885913/151‑1 (MQ=255)
                                                                   gAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGAc                                                                                <  1:1516970/150‑1 (MQ=255)
                                                                   gAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACa                                                                               <  2:367106/151‑1 (MQ=255)
                                                                     ggATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACaaa                                                                             <  1:1177349/151‑1 (MQ=255)
                                                                        tAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAAc                                                                            >  2:326458/1‑149 (MQ=255)
                                                                         aaTTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAg                                                                          <  2:1174535/150‑1 (MQ=255)
                                                                          aTTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCa                                                                        <  1:907372/151‑1 (MQ=255)
                                                                           tttGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCAt                                                                       >  2:289271/1‑151 (MQ=255)
                                                                           tttGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCAt                                                                       >  2:1408395/1‑151 (MQ=255)
                                                                            ttGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCAt                                                                       <  2:2034877/150‑1 (MQ=255)
                                                                            ttGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCAt                                                                       >  2:1467495/1‑150 (MQ=255)
                                                                             tgtgCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATa                                                                      <  1:1205016/150‑1 (MQ=255)
                                                                                gCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAACTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTg                                                                  <  1:105762/151‑1 (MQ=255)
                                                                                  cATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGaa                                                                <  1:2151486/151‑1 (MQ=255)
                                                                                      tcCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTg                                                             >  2:2044954/1‑150 (MQ=255)
                                                                                       ccACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTg                                                             <  2:1743809/149‑1 (MQ=255)
                                                                                        cACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACg                                                          >  1:1867021/1‑151 (MQ=255)
                                                                                         aCCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGt                                                         <  1:3479/151‑1 (MQ=255)
                                                                                           cgccgcCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTAt                                                       <  2:64899/151‑1 (MQ=255)
                                                                                            gccgccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTAtt                                                      >  2:1237584/1‑151 (MQ=255)
                                                                                             ccgccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTAttt                                                     >  1:1916888/1‑151 (MQ=255)
                                                                                             ccgccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTAttt                                                     <  2:1604764/151‑1 (MQ=255)
                                                                                               gccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTAc                                                   >  2:357332/1‑151 (MQ=255)
                                                                                               gccAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTAc                                                   >  2:658889/1‑151 (MQ=255)
                                                                                                 cAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTg                                                 >  2:1589793/1‑151 (MQ=255)
                                                                                                 cAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTg                                                 <  2:1805109/151‑1 (MQ=255)
                                                                                                   gcgcCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGc                                               <  2:1539229/151‑1 (MQ=255)
                                                                                                     gcCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCAt                                             <  2:2019709/151‑1 (MQ=255)
                                                                                                      ccaccaccaGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATc                                            <  1:420147/151‑1 (MQ=255)
                                                                                                       caccaccaGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATcc                                           <  1:1826303/151‑1 (MQ=255)
                                                                                                             caGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGAc                                     <  2:296740/151‑1 (MQ=255)
                                                                                                             caGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGAc                                     <  1:201968/151‑1 (MQ=255)
                                                                                                              aGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACt                                    >  1:634142/1‑151 (MQ=255)
                                                                                                               gCCCGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTg                                   <  2:1056860/151‑1 (MQ=255)
                                                                                                                 ccGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGAc                                 >  2:910212/1‑151 (MQ=255)
                                                                                                                 ccGACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGAc                                 <  2:171354/151‑1 (MQ=255)
                                                                                                                   gACCACCAGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGAcc                                >  1:903006/1‑150 (MQ=255)
                                                                                                                        ccaGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAg                          <  1:2163209/151‑1 (MQ=255)
                                                                                                                         caGATAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAAc                            >  1:245671/1‑148 (MQ=255)
                                                                                                                             tAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGAt                        >  2:1292852/1‑148 (MQ=255)
                                                                                                                             tAAAAGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGt                     >  1:364161/1‑151 (MQ=255)
                                                                                                                               aaaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGtt                    >  2:789802/1‑150 (MQ=255)
                                                                                                                                aaGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTa                   >  2:323246/1‑150 (MQ=255)
                                                                                                                                 aGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTaaa                 <  2:409831/151‑1 (MQ=255)
                                                                                                                                 aGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTaaa                 <  1:1909859/151‑1 (MQ=255)
                                                                                                                                 aGAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTaa                  >  2:1991550/1‑150 (MQ=255)
                                                                                                                                  gAAACGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAc                >  1:150920/1‑151 (MQ=255)
                                                                                                                                    aaCGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGc              >  1:244574/1‑151 (MQ=255)
                                                                                                                                    aaCGTAATATCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGc              >  2:660671/1‑151 (MQ=255)
                                                                                                                                            atCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTc      >  1:788909/1‑151 (MQ=255)
                                                                                                                                            atCTTGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTc      >  1:1363763/1‑151 (MQ=255)
                                                                                                                                                tGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTcc     <  2:414545/148‑1 (MQ=255)
                                                                                                                                                tGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTc      <  2:1593081/147‑1 (MQ=255)
                                                                                                                                                tGCAATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCcgc  >  2:807036/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAGCACATAGCTGTTGCTGTAAATCAGATATTTCGAAATCGACTCCATAATACTGCTGCCACCGGTGAGGATAATTTGTGCCATCTCCACCGCCGCCAGCGCCACCACCAGCCCGACCACCAGATAAAAGAAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGC  >  NC_000913/2130398‑2130692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑