Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 216,672 A→G V111A (GTC→GCC)  yaeF ← putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913216,6720AG99.2% 514.4 / ‑4.6 134V111A (GTC→GCC) yaeFputative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (75/58);  total (76/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 135 total aligned reads displayed.

GACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGACGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGG  >  NC_000913/216524‑216817
                                                                                                                                                    |                                                                                                                                                 
gACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGTGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCg                                                                                                                                                 <  2:2104505/151‑1 (MQ=255)
gACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCg                                                                                                                                                 >  2:932433/1‑151 (MQ=255)
  cAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGcc                                                                                                                                               >  2:1644484/1‑151 (MQ=255)
   aaTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGcc                                                                                                                                               >  2:554428/1‑150 (MQ=255)
   aaTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGcc                                                                                                                                               >  2:1509054/1‑150 (MQ=255)
   aaTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGcc                                                                                                                                               >  2:1084898/1‑150 (MQ=255)
   aaTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCa                                                                                                                                              <  2:1346859/151‑1 (MQ=255)
    aTGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAg                                                                                                                                             <  1:1766135/151‑1 (MQ=255)
     tGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAgc                                                                                                                                            >  2:1314553/1‑151 (MQ=255)
      gCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAgcg                                                                                                                                           >  2:328743/1‑151 (MQ=255)
      gCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAgcg                                                                                                                                           <  1:365135/151‑1 (MQ=255)
         gcgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCt                                                                                                                                        <  1:1767781/151‑1 (MQ=255)
          cgATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGACGCCAGCGCCTg                                                                                                                                       >  2:483437/1‑151 (MQ=255)
           gATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGt                                                                                                                                      >  2:1968442/1‑151 (MQ=255)
           gATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGt                                                                                                                                      <  1:1189765/151‑1 (MQ=255)
            aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                     >  1:1624876/1‑151 (MQ=255)
            aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                     >  2:1794381/1‑151 (MQ=255)
            aTAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTc                                                                                                                                     >  2:544376/1‑151 (MQ=255)
              aGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGc                                                                                                                                   >  1:1896188/1‑151 (MQ=255)
                 tataACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTc                                                                                                                                >  1:539240/1‑151 (MQ=255)
                  ataACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCt                                                                                                                               >  1:1602169/1‑151 (MQ=255)
                  ataACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCt                                                                                                                               >  2:1569539/1‑151 (MQ=255)
                    aaCCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGc                                                                                                                             >  1:1501524/1‑151 (MQ=255)
                     aCCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCa                                                                                                                            <  1:2131479/151‑1 (MQ=255)
                           aTCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtt                                                                                                                       <  2:610827/150‑1 (MQ=255)
                            tCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGttat                                                                                                                     >  2:4019/1‑151 (MQ=255)
                             cTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGttatt                                                                                                                    >  1:182185/1‑151 (MQ=255)
                               ttGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGttatta                                                                                                                   <  2:1863355/150‑1 (MQ=255)
                               ttGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtta                                                                                                                      <  2:859600/147‑1 (MQ=255)
                               ttGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGtta                                                                                                                      <  1:958321/147‑1 (MQ=255)
                                 gATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATc                                                                                                                 >  1:1294206/1‑150 (MQ=255)
                                    tttATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCAccc                                                                                                             >  2:529416/1‑151 (MQ=255)
                                     ttATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCAccc                                                                                                             >  2:87817/1‑150 (MQ=255)
                                      tATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCAccc                                                                                                             >  2:903604/1‑149 (MQ=255)
                                        ttGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGa                                                                                                          >  1:1036113/1‑150 (MQ=255)
                                         tGGCAAACGCGGTGATATCTGTGGCTTGGTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGaa                                                                                                         >  1:152024/1‑150 (MQ=255)
                                            cAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGAt                                                                                                     <  1:580881/151‑1 (MQ=255)
                                                  cgGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAAc                                                                                               <  2:1629178/151‑1 (MQ=255)
                                                  cgGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAAc                                                                                               >  1:1345190/1‑151 (MQ=255)
                                                   ggTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACg                                                                                              <  1:1379428/151‑1 (MQ=255)
                                                      gATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTg                                                                                            >  1:845112/1‑150 (MQ=255)
                                                      gATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTg                                                                                            >  1:440018/1‑150 (MQ=255)
                                                      gATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGa                                                                                           <  1:432864/151‑1 (MQ=255)
                                                       atatCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGAc                                                                                          >  1:1426469/1‑151 (MQ=255)
                                                         atCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACt                                                                                         <  1:1720787/150‑1 (MQ=255)
                                                         atCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTc                                                                                        >  1:901634/1‑151 (MQ=255)
                                                          tCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTc                                                                                        >  1:1163058/1‑150 (MQ=255)
                                                            tgtgGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTcac                                                                                      >  1:326735/1‑150 (MQ=255)
                                                             gtgGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTcac                                                                                      <  2:1022767/149‑1 (MQ=255)
                                                             gtgGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTcac                                                                                      >  2:1566527/1‑149 (MQ=255)
                                                             gtgGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTca                                                                                       <  2:140913/148‑1 (MQ=255)
                                                             gtgGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACag                                                                                    <  2:2124791/151‑1 (MQ=255)
                                                                   tgttgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGaa                                                                               <  1:1699051/150‑1 (MQ=255)
                                                                   tgttgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGaa                                                                               <  1:1644515/150‑1 (MQ=255)
                                                                   tgttgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGa                                                                                <  1:80860/149‑1 (MQ=255)
                                                                    gttgCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGt                                                                             <  1:103678/151‑1 (MQ=255)
                                                                         ggggTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTg                                                                         >  2:196299/1‑150 (MQ=255)
                                                                           ggTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAg                                                                      <  1:944414/151‑1 (MQ=255)
                                                                            gTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGa                                                                     <  1:601112/151‑1 (MQ=255)
                                                                              aaGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGAcg                                                                   >  2:1105191/1‑151 (MQ=255)
                                                                                 aTCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCgg                                                                <  1:1379663/151‑1 (MQ=255)
                                                                                   ccGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGAt                                                              <  2:1676647/151‑1 (MQ=255)
                                                                                    cGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGAtt                                                             >  2:1139975/1‑151 (MQ=255)
                                                                                                gACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGtt                                                 >  2:514193/1‑151 (MQ=255)
                                                                                                 aCGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGtt                                                 >  2:1200004/1‑150 (MQ=255)
                                                                                                 aCGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGtt                                                 >  1:1904908/1‑150 (MQ=255)
                                                                                                 aCGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa                                                <  2:185520/151‑1 (MQ=255)
                                                                                                   gAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAcc                                              <  1:1299652/151‑1 (MQ=255)
                                                                                                     aaaGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAcccc                                            >  2:2169467/1‑151 (MQ=255)
                                                                                                       aGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCa                                           >  2:1779118/1‑150 (MQ=255)
                                                                                                         cTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAgg                                        >  2:629573/1‑151 (MQ=255)
                                                                                                          ttATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGc                                       >  2:1391646/1‑151 (MQ=255)
                                                                                                           tATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCt                                      >  1:1987278/1‑151 (MQ=255)
                                                                                                            aTCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCt                                      <  2:1616675/150‑1 (MQ=255)
                                                                                                              cACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTg                                    >  2:949031/1‑150 (MQ=255)
                                                                                                                 tATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCtt                                     >  1:880255/1‑146 (MQ=255)
                                                                                                                 tATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCtt                                     <  2:880243/146‑1 (MQ=255)
                                                                                                                    gCTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGaa                             <  1:932446/151‑1 (MQ=255)
                                                                                                                     cTTCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAAc                            >  1:1915622/1‑151 (MQ=255)
                                                                                                                       tCATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACa                           <  2:78510/150‑1 (MQ=255)
                                                                                                                        cATCGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGc                         >  2:1252496/1‑151 (MQ=255)
                                                                                                                           cGCTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCaa                       <  1:2021062/150‑1 (MQ=255)
                                                                                                                             cTTTTTTAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATc                    >  1:1493731/1‑151 (MQ=255)
                                                                                                                               tttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATc                    <  1:694526/149‑1 (MQ=255)
                                                                                                                               tttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCg                   <  1:1185665/150‑1 (MQ=255)
                                                                                                                               tttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                  >  2:397155/1‑151 (MQ=255)
                                                                                                                               tttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                  >  1:1977408/1‑151 (MQ=255)
                                                                                                                                ttttAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGc                  <  1:2161111/150‑1 (MQ=255)
                                                                                                                                  ttAAGGGGAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCgg               >  2:1882008/1‑151 (MQ=255)
                                                                                                                                   tAAGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCgg               <  2:259314/150‑1 (MQ=255)
                                                                                                                                    aaGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCggg              <  1:1065113/150‑1 (MQ=255)
                                                                                                                                    aaGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGt             <  2:539234/151‑1 (MQ=255)
                                                                                                                                     aGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGtt            >  2:1458898/1‑151 (MQ=255)
                                                                                                                                     aGGGAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGtt            <  2:1584147/151‑1 (MQ=255)
                                                                                                                                        gAAACGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga         <  2:1487305/151‑1 (MQ=255)
                                                                                                                                          aaCGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTg          >  2:587888/1‑148 (MQ=255)
                                                                                                                                           aCGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTg          >  2:1963277/1‑147 (MQ=255)
                                                                                                                                           aCGATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGAt      <  2:845101/151‑1 (MQ=255)
                                                                                                                                             gATCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAAACGCCGGGTTTGAGATc     >  2:134506/1‑150 (MQ=255)
                                                                                                                                               tCTGGGCGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAgg  >  2:2046685/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GACAATGCCGCGATAGTTATAACCGCTATCTTTGATTTTATTGGCAAACGCGGTGATATCTGTGGCTTGTTGCGGGGTAAGATCCGGGACTCGTAAGACGAAAAGCTTATCACTATGCTTCATCGCTTTTTTAAGGGAAACGATCTGGACGCCAGCGCCTGTCGCTTCTGCAACGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGG  >  NC_000913/216524‑216817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: