Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,224,823 A→G A16A (GCT→GCC pbpG ← D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,224,8230AG100.0% 524.6 / NA 136A16A (GCT→GCCpbpGD‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (64/72);  total (64/72)

Only 100 of 142 total aligned reads displayed.

AATCGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGA  >  NC_000913/2224676‑2224970
                                                                                                                                                   |                                                                                                                                                   
   cGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCAcggccagc                                                                                                                                               >  2:1095575/1‑151 (MQ=255)
   cGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCAcggccagc                                                                                                                                               >  2:1793428/1‑151 (MQ=255)
    ggACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCAcggccagc                                                                                                                                               >  2:199764/1‑150 (MQ=255)
     gACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCAt                                                                                                                                             >  1:739540/1‑151 (MQ=255)
         cACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAgg                                                                                                                                         <  1:1607638/151‑1 (MQ=255)
         cACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAgg                                                                                                                                         >  1:1975653/1‑151 (MQ=255)
          aCCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAggg                                                                                                                                        >  1:938070/1‑151 (MQ=255)
           ccAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGc                                                                                                                                       <  1:839318/151‑1 (MQ=255)
            cAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGcc                                                                                                                                      <  2:900175/151‑1 (MQ=255)
            cAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGcc                                                                                                                                      >  1:128905/1‑151 (MQ=255)
             aGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCa                                                                                                                                     <  1:1890476/151‑1 (MQ=255)
              gATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAg                                                                                                                                    <  1:1355612/151‑1 (MQ=255)
               aTCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAgg                                                                                                                                   <  2:896832/151‑1 (MQ=255)
                tCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAgg                                                                                                                                   >  2:1156265/1‑150 (MQ=255)
                 ccGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGc                                                                                                                                  <  1:111527/150‑1 (MQ=255)
                 ccGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCt                                                                                                                                 >  1:1027790/1‑151 (MQ=255)
                 ccGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCt                                                                                                                                 <  1:2012641/151‑1 (MQ=255)
                   gggTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCta                                                                                                                                >  2:746740/1‑150 (MQ=255)
                     gtggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaat                                                                                                                             >  1:2140455/1‑151 (MQ=255)
                     gtggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaat                                                                                                                             >  2:1062226/1‑151 (MQ=255)
                     gtggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaat                                                                                                                             <  2:281740/151‑1 (MQ=255)
                     gtggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaat                                                                                                                             <  2:299557/151‑1 (MQ=255)
                      tggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaat                                                                                                                             >  1:560210/1‑150 (MQ=255)
                       ggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaata                                                                                                                            >  1:557947/1‑150 (MQ=255)
                       ggtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaata                                                                                                                            <  1:1271036/150‑1 (MQ=255)
                        gtTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCtaaataa                                                                                                                           >  2:971520/1‑150 (MQ=255)
                          tCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAg                                                                                                                         <  2:1453337/150‑1 (MQ=255)
                           cGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGaa                                                                                                                       <  1:1476528/151‑1 (MQ=255)
                           cGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGa                                                                                                                        >  2:2022556/1‑150 (MQ=255)
                            gAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGaaa                                                                                                                      <  2:1261402/151‑1 (MQ=255)
                             aaTAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAAc                                                                                                                     >  1:1862741/1‑151 (MQ=255)
                                  aTCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGaa                                                                                                                <  1:2168253/151‑1 (MQ=255)
                                  aTCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGaa                                                                                                                <  2:1913240/151‑1 (MQ=255)
                                  aTCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGa                                                                                                                 >  2:1905517/1‑150 (MQ=255)
                                     aCTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAAtt                                                                                                             >  1:784711/1‑151 (MQ=255)
                                      cTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAAttt                                                                                                            >  2:175655/1‑151 (MQ=255)
                                        ttgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAAttt                                                                                                            <  2:1102162/149‑1 (MQ=255)
                                        ttgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTc                                                                                                           <  2:1868291/150‑1 (MQ=255)
                                        ttgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTc                                                                                                           <  2:1119777/150‑1 (MQ=255)
                                        ttgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTc                                                                                                           <  2:590510/150‑1 (MQ=255)
                                         tgttgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCgg                                                                                                         >  2:714522/1‑151 (MQ=255)
                                            tgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCat                                                                                                      >  2:239926/1‑151 (MQ=255)
                                            tgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCat                                                                                                      >  2:1323383/1‑151 (MQ=255)
                                            tgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCa                                                                                                       <  1:175656/150‑1 (MQ=255)
                                            tgGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCa                                                                                                       <  1:984937/150‑1 (MQ=255)
                                             ggTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCat                                                                                                      <  1:1650487/150‑1 (MQ=255)
                                              gTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCatg                                                                                                     <  2:1106752/150‑1 (MQ=255)
                                                aTTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCatgatg                                                                                                  >  2:1335421/1‑151 (MQ=255)
                                                  tCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCatgatg                                                                                                  >  2:1457151/1‑149 (MQ=255)
                                                   cAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAg                                                                                                <  1:963755/150‑1 (MQ=255)
                                                    aGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGc                                                                                               >  1:779881/1‑150 (MQ=255)
                                                    aGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGc                                                                                               >  2:76953/1‑150 (MQ=255)
                                                      aTCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCAtt                                                                                            >  2:384742/1‑151 (MQ=255)
                                                       tCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTc                                                                                           >  2:1285898/1‑151 (MQ=255)
                                                        caacaaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTc                                                                                           >  1:149776/1‑150 (MQ=255)
                                                           caaTCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTc                                                                                           >  2:1982430/1‑147 (MQ=255)
                                                              tcatcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGt                                                                                    >  2:438066/1‑151 (MQ=255)
                                                               catcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGt                                                                                    <  2:1584954/150‑1 (MQ=255)
                                                                atcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTgg                                                                                  >  2:1791555/1‑151 (MQ=255)
                                                                atcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTgg                                                                                  >  1:940454/1‑151 (MQ=255)
                                                                atcGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTgg                                                                                  >  2:1239580/1‑151 (MQ=255)
                                                                  cgcgCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGtt                                                                                <  1:1380256/151‑1 (MQ=255)
                                                                  cgcgCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGtt                                                                                >  1:1333043/1‑151 (MQ=255)
                                                                      cTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCAcg                                                                            >  2:1456485/1‑151 (MQ=255)
                                                                        aCCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCAcgcg                                                                          <  2:1560614/151‑1 (MQ=255)
                                                                        aCCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCAcgc                                                                           <  2:28312/150‑1 (MQ=255)
                                                                          cGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACgcgc                                                                         >  1:396464/1‑150 (MQ=255)
                                                                            gAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACg                                                                      <  2:1858276/151‑1 (MQ=255)
                                                                             aGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACgg                                                                     <  2:1019025/151‑1 (MQ=255)
                                                                                       cGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCAcc                                                           >  1:1765129/1‑151 (MQ=255)
                                                                                        ggTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCAccg                                                          >  1:358002/1‑151 (MQ=255)
                                                                                            gtgAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCgg                                                      >  1:548056/1‑151 (MQ=255)
                                                                                              gAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAg                                                    <  2:1330080/151‑1 (MQ=255)
                                                                                               aaGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGt                                                   >  2:1772505/1‑151 (MQ=255)
                                                                                                  cGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAg                                                <  1:1849194/151‑1 (MQ=255)
                                                                                                   ggtggtAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAgg                                               >  2:215852/1‑151 (MQ=255)
                                                                                                         aGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTAc                                         <  1:589936/151‑1 (MQ=255)
                                                                                                          gCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACt                                        >  2:338725/1‑151 (MQ=255)
                                                                                                            ggCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTg                                       >  2:1722717/1‑150 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGc                                   <  1:605030/150‑1 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGc                                   <  1:2023945/150‑1 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGc                                   <  1:971534/150‑1 (MQ=255)
                                                                                                                gCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCt                                  >  1:1604114/1‑151 (MQ=255)
                                                                                                                  cGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAg                                <  1:994340/151‑1 (MQ=255)
                                                                                                                      ttCGCAACGGCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGAc                            >  2:858510/1‑151 (MQ=255)
                                                                                                                              ggCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatcat                    <  1:1556816/151‑1 (MQ=255)
                                                                                                                              ggCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatcat                    <  2:295160/151‑1 (MQ=255)
                                                                                                                              ggCCTGCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCcatcat                    <  2:2140410/151‑1 (MQ=255)
                                                                                                                                   gCGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACg                <  1:533971/150‑1 (MQ=255)
                                                                                                                                    cGGTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAg              <  2:1272661/151‑1 (MQ=255)
                                                                                                                                      gTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGc             >  1:538756/1‑150 (MQ=255)
                                                                                                                                      gTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGc             >  1:835418/1‑150 (MQ=255)
                                                                                                                                      gTGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCa            <  2:536519/151‑1 (MQ=255)
                                                                                                                                       tGCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCa            >  2:699594/1‑150 (MQ=255)
                                                                                                                                        gCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaa          <  2:938057/151‑1 (MQ=255)
                                                                                                                                        gCAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaa           <  2:1366520/150‑1 (MQ=255)
                                                                                                                                         cAAAAGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaa         <  1:1351680/151‑1 (MQ=255)
                                                                                                                                          aaaaGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCaaaaa        <  1:109684/151‑1 (MQ=255)
                                                                                                                                             aGGCACGGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTg     <  1:2137986/151‑1 (MQ=255)
                                                                                                                                                cacgGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGa  <  1:1437564/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AATCGGACGCACCAGATCCGGGTGGTTCGAATAGATCACTTTGTTGGTATTCAGATCAACAATCATCGCGCTACCGGAGGCAATTTCCGGTTGTGAAGCGGTGGTAGCGGCTGCCGTTTTCGCAACGGCCTGCGGTGCAAAAGGCACAGCCAGCATCAGGGCCAGGCTAAATAAAGAAACTCGAAATTTCGGCATGATGAGCATTCAGATAGTGGTTCACGCGCACGGGTTGCGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGA  >  NC_000913/2224676‑2224970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: