Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,693	G→A	A21V (GCG→GTG)	mqo ←	malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,693	0	G	A	100.0%	388.1 / NA	97	A21V (GCG→GTG)	mqo	malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/67);  total (30/67)

GCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAAT  >  NC_000913/2306547‑2306840                                                                                                                                                   |                                                                                                                                                    gcgcGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGccacca                                                                                                                                                  <  1:1018061/150‑1 (MQ=255)       cacCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAAc                                                                                                                                           >  1:778512/1‑151 (MQ=255)       cacCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAAc                                                                                                                                           >  2:132466/1‑151 (MQ=255)        acCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACg                                                                                                                                          >  1:979561/1‑151 (MQ=255)         cccTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACgg                                                                                                                                         <  2:993661/151‑1 (MQ=255)          ccTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGc                                                                                                                                        <  2:326498/151‑1 (MQ=255)              cAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTa                                                                                                                                    <  1:1363765/151‑1 (MQ=255)                      ccaccaTGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCa                                                                                                                             <  1:1063180/150‑1 (MQ=255)                      ccaccaTGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCAc                                                                                                                            <  1:494653/151‑1 (MQ=255)                      ccaccaTGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCAc                                                                                                                            <  2:1442920/151‑1 (MQ=255)                       caccaTGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACg                                                                                                                           >  1:760040/1‑151 (MQ=255)                           aTGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCa                                                                                                                       <  2:85156/151‑1 (MQ=255)                              gTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCg                                                                                                                    <  2:2127034/151‑1 (MQ=255)                                cATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCgag                                                                                                                  <  1:1175765/151‑1 (MQ=255)                                cATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCga                                                                                                                   <  2:2158728/150‑1 (MQ=255)                                           cAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGc                                                                                                       >  1:1667452/1‑151 (MQ=255)                                            aGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCa                                                                                                      <  1:132466/151‑1 (MQ=255)                                              gCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGt                                                                                                    <  1:1286649/151‑1 (MQ=255)                                                  cAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACt                                                                                                <  1:2046432/151‑1 (MQ=255)                                                     cTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttt                                                                                             >  2:789706/1‑151 (MQ=255)                                                       cgcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttttt                                                                                           <  2:146924/151‑1 (MQ=255)                                                       cgcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttttt                                                                                           <  2:1849164/151‑1 (MQ=255)                                                       cgcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttt                                                                                             <  2:1630702/149‑1 (MQ=255)                                                       cgcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttt                                                                                             <  1:600125/149‑1 (MQ=255)                                                       cgcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCACTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACttttt                                                                                            <  2:1632717/150‑1 (MQ=255)                                                        gcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTc                                                                                          <  2:1708502/151‑1 (MQ=255)                                                        gcgTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTc                                                                                          >  1:1758638/1‑151 (MQ=255)                                                          gTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCAt                                                                                        <  1:1644141/151‑1 (MQ=255)                                                          gTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCAt                                                                                        <  1:1456295/151‑1 (MQ=255)                                                          gTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCAt                                                                                        >  1:436079/1‑151 (MQ=255)                                                           tAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGTCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCAt                                                                                        <  2:1148452/150‑1 (MQ=255)                                                           tAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACtttt                                                                                             <  1:805274/145‑1 (MQ=255)                                                           tAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATc                                                                                       >  1:1385208/1‑151 (MQ=255)                                                             aaTAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCtt                                                                                     <  1:762046/151‑1 (MQ=255)                                                             aaTAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCtt                                                                                     <  1:348265/151‑1 (MQ=255)                                                                aGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGtt                                                                                  <  1:996272/151‑1 (MQ=255)                                                                aGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGt                                                                                   <  2:1081669/150‑1 (MQ=255)                                                                 ggTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGtt                                                                                  >  2:379236/1‑150 (MQ=255)                                                                 ggTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGtt                                                                                  >  2:1715566/1‑150 (MQ=255)                                                                      ccAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGcc                                                                            <  2:1212548/151‑1 (MQ=255)                                                                          cGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGTAGTCACTTTTTTCATCTTGTTAATGCCTTa                                                                         >  1:1502211/1‑150 (MQ=255)                                                                          cGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTAc                                                                        <  2:1385172/151‑1 (MQ=255)                                                                          cGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTAc                                                                        <  2:2142044/151‑1 (MQ=255)                                                                           gTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACt                                                                       <  1:1515499/151‑1 (MQ=255)                                                                           gTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACt                                                                       >  1:1689072/1‑151 (MQ=255)                                                                             ggCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACtt                                                                      <  2:666400/150‑1 (MQ=255)                                                                             ggCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACtt                                                                      <  2:188148/150‑1 (MQ=255)                                                                              gcgcTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACtttt                                                                    <  1:1103374/151‑1 (MQ=255)                                                                               cgcTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTa                                                                   <  2:1019910/151‑1 (MQ=255)                                                                               cgcTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTa                                                                   <  2:498525/151‑1 (MQ=255)                                                                                   cATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCg                                                               <  1:981397/151‑1 (MQ=255)                                                                                       aTGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCttt                                                           >  1:1002468/1‑151 (MQ=255)                                                                                         gccgccgccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTAt                                                         >  2:1457782/1‑151 (MQ=255)                                                                                         gccgccgccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTAt                                                         <  2:436074/151‑1 (MQ=255)                                                                                             ccgccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCCCGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCa                                                     <  1:71478/151‑1 (MQ=255)                                                                                             ccgccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCa                                                     <  2:334259/151‑1 (MQ=255)                                                                                               gccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAgg                                                   >  2:292033/1‑151 (MQ=255)                                                                                                ccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGt                                                  >  2:83573/1‑151 (MQ=255)                                                                                                ccAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGt                                                  <  2:1417751/151‑1 (MQ=255)                                                                                                  aaTCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTg                                                 >  1:496531/1‑150 (MQ=255)                                                                                                        cAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCAGCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTg                                          <  1:983714/151‑1 (MQ=255)                                                                                                         aGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTgc                                         <  1:1922716/151‑1 (MQ=255)                                                                                                         aGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTgc                                         <  1:169728/151‑1 (MQ=255)                                                                                                          gTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTgcg                                        <  2:758050/151‑1 (MQ=255)                                                                                                            aCATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTgcgc                                       <  2:1404165/150‑1 (MQ=255)                                                                                                            aCATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTgcgc                                       <  2:420735/150‑1 (MQ=255)                                                                                                            aCATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGcgcg                                      <  2:1347876/151‑1 (MQ=255)                                                                                                            aCATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGcgcg                                      >  2:2016807/1‑151 (MQ=255)                                                                                                              aTCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGc                                    >  2:960039/1‑151 (MQ=255)                                                                                                                cAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCAt                                  >  2:1231385/1‑151 (MQ=255)                                                                                                                 aGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGACCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATc                                 <  2:1542830/151‑1 (MQ=255)                                                                                                                   tttCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCa                                <  1:379238/150‑1 (MQ=255)                                                                                                                    ttCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAAc                              >  1:290841/1‑151 (MQ=255)                                                                                                                    ttCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAAc                              >  2:1283950/1‑151 (MQ=255)                                                                                                                    ttCCTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTGTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCaa                               <  2:1523839/150‑1 (MQ=255)                                                                                                                      ccTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACgg                            >  1:716912/1‑151 (MQ=255)                                                                                                                       cTGCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGt                           >  1:2018840/1‑151 (MQ=255)                                                                                                                         gCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGt                           <  1:351455/149‑1 (MQ=255)                                                                                                                         gCTCCTCGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGt                           <  2:2018797/149‑1 (MQ=255)                                                                                                                           tcctcGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTaa                         >  1:486461/1‑149 (MQ=255)                                                                                                                            cctcGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAacac                      <  2:1758606/151‑1 (MQ=255)                                                                                                                             ctcGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACt                     >  1:1164814/1‑151 (MQ=255)                                                                                                                               cGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACtt                    >  1:1105830/1‑150 (MQ=255)                                                                                                                               cGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACtt                    <  1:2045788/150‑1 (MQ=255)                                                                                                                               cGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACt                     <  2:2103216/149‑1 (MQ=255)                                                                                                                               cGGACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTa                   <  1:632453/151‑1 (MQ=255)                                                                                                                                ggACGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTa                   >  1:1776453/1‑150 (MQ=255)                                                                                                                                  aCGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGt                <  1:1257930/151‑1 (MQ=255)                                                                                                                                  aCGCTTTCGCTTTTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGt                <  1:215446/151‑1 (MQ=255)                                                                                                                                   cgctttcgctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTa               <  1:663968/151‑1 (MQ=255)                                                                                                                                   cgctttcgctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTa               <  1:415686/151‑1 (MQ=255)                                                                                                                                        tcgctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTaacaa           <  1:377931/150‑1 (MQ=255)                                                                                                                                         cgctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTaacaac          <  1:899411/150‑1 (MQ=255)                                                                                                                                          gctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAacaacac        <  2:998363/151‑2 (MQ=255)                                                                                                                                          gctttTGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACAt        >  2:1703059/1‑151 (MQ=255)                                                                                                                                             tttGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACAtt       <  1:1626204/149‑1 (MQ=255)                                                                                                                                             tttGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACAtt       <  2:1936254/149‑1 (MQ=255)                                                                                                                                             tttGCCACCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACAtt       <  2:2005305/149‑1 (MQ=255)                                                                                                                                                gccaccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAaat  <  2:671990/151‑1 (MQ=255)                                                                                                                                                gccaccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAaat  <  2:344652/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCGCGACACCCTCCAGGCGCTCCACCATGGTCATCGACCATTCAGGCTCCAGCTCGCGTAAATAGGTCCCCAACGTGGCGCTCATAATGCCGCCGCCAATCAACAGTACATCAGTTTCCTGCTCCTCGGACGCTTTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAAT  >  NC_000913/2306547‑2306840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑