Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,8530AC51.8% 65.8 / 88.7 57S725A (TCA→GCA) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base A (21/6);  new base C (0/29);  total (21/36)
Fisher's exact test for biased strand distribution p-value = 2.20e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-09

GCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTA  >  NC_000913/3350708‑3350996
                                                                                                                                                   |                                                                                                                                               
gCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccg                                                                                                                                            <  2:729478/151‑1 (MQ=255)
    cccacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCCCCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCccgccg                                                                                                                                         <  1:155357/150‑1 (MQ=255)
      cacGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCAc                                                                                                                                      <  2:309884/151‑1 (MQ=255)
        cGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCt                                                                                                                                    >  2:424244/1‑151 (MQ=255)
           tttCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCttt                                                                                                                                  >  1:102698/1‑150 (MQ=255)
                 cACTTCTACGTCGTGCCCCCACTCTTTTTTCATCTTTCCAACCCATCCCCCGCCGTTATCTCCCCAGCCGGGAAGGTCAGGAGACTGATTTTGCTGCCCCATTTGTTGCAGATGGGGAAACCCCACGGCCCCCGCCGCACCTTTAAttt                                                                                                                             <  1:567639/149‑1 (MQ=255)
                            cgtGCCCCCCCTCTTCTTTCTTCTCTTCAATCCATTCCCCGACTTTATCTTCCCGGCCGGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGTTGTTGAAGTTGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGtccttc                                                                                                                   <  2:138907/148‑1 (MQ=255)
                            cgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTc                                                                                                                >  1:164979/1‑151 (MQ=255)
                              tgccccccctctTCTTTCATCTCTACACCCCATCCCCCGCCTTTTTCTCCCCAGGCGGGAGGGTAAGGAGACTAATTTTGCTGCCCCAGTTTTTGCAGATGGCTTCCCCCCACTGACCCCGCCGCACCTTTAATTTTATGtccttcct                                                                                                                 <  1:393689/140‑1 (MQ=255)
                                 ccccccttttcttTCATCCCTCCAACCCATCCCCCGACTTTATCTCCCCAGGCCGAAGGGAAGGGAGACTGATTTTCCTCACCCAGTTTTTGGAGATGCCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaa                                                                                                              <  1:833674/140‑1 (MQ=255)
                                  ccctctctTCTTTCATCTCTCCACCCCATCCCCCGGTTTTATCTCCCCAGCCCGGAGGGTCAGAAGACTATTTTTGCTCCCCCAGTTGTTGCAGTGGGTGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAAt                                                                                                          <  1:973065/147‑1 (MQ=255)
                                      ctctTCTTTCATCTCTTCAACCCATCCCCCGACTTTATCTCCCCAGGCCGGAAGGCCAGGAGACTAATTTTGCCGCCCCTTCTTTGGAGGATGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGc                                                                                                        <  1:979483/149‑1 (MQ=255)
                                       tcttctTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtt                                                                                                     >  2:422850/1‑151 (MQ=255)
                                         ttcttcCTTCTTTTCAACCCATCCCCCGACTTTTTCTCCCCAGCCGGGAGGGTCGGGAGACTGAATTTGCTGCCCCGGTTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCt                                                                                                      <  2:1002187/148‑1 (MQ=255)
                                         ttcttTCATCTCTCCAACCCATCCACCGACGTTATCTCCCCAGGCCGGAGGGTCAGGAGACTGAATTTGCTGCCCCAGTTGTTGAAGATGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATg                                                                                                         <  2:1202227/145‑1 (MQ=255)
                                             ttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                               >  1:228004/1‑151 (MQ=255)
                                             ttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                               >  1:104678/1‑151 (MQ=255)
                                             tccATCTCTCAAACCCATCCCCCGCCTTTATCTCCCCAGGCGGGAGGGCAGGAAGACTAATTTTGCTGCCCCATTTTTTGCAGTTGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                                 <  1:203560/147‑1 (MQ=255)
                                                aTCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCt                                                                                            >  2:1044142/1‑151 (MQ=255)
                                                  ctctacatcccATCCTCCGACTTCTTCTTCCCAGGCCGGAGGGTCAGGGGATTAATTTTCCTGCCCCATTTTTTTAAGTTGCCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTa                                                                                                <  1:159178/136‑1 (MQ=255)
                                                  ctctTCAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGAAGATGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCt                                                                                            <  2:771521/149‑1 (MQ=255)
                                                    ctTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcgc                                                                                        >  2:519181/1‑151 (MQ=255)
                                                     ttaaacccATCCCCCGACTTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAATTTTGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgc                                                                                          <  2:392701/142‑1 (MQ=255)
                                                        aaTCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                    >  2:1179994/1‑151 (MQ=255)
                                                        aaTCCATCCCCCGCCGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTAAATTTGCTGACCCAGCTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                    <  2:1172244/151‑1 (MQ=255)
                                                            cATTCCCCGCCTTTTTCTCCCCAGGCCGAAAGGTCAGGAGACTAAATTTGCTGCCCCATCTGTTGCAGGTGGCGTACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGa                                                                                 <  1:224121/150‑1 (MQ=255)
                                                              tcccccGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGCCTGAATTTGCTCCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCa                                                                                   <  2:595947/142‑1 (MQ=255)
                                                              tcccccGACGTTATCTCCCCGGGCGGGAGGGTGAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGAtt                                                                               <  2:936054/146‑1 (MQ=255)
                                                                     aCGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTcc                                                                        >  2:613289/1‑150 (MQ=255)
                                                                        ttATCTCCCCAGGCCGGAGGGTCAGGAGACTGATTTTGCTGCCCCATTTTTTGCAGGGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGc                                                                     <  1:376149/150‑1 (MQ=255)
                                                                         tATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCAc                                                                   >  2:780221/1‑151 (MQ=255)
                                                                              tCCCAGGCCCGGAAGGTAAGGAGATTGATTTTCCTACCCCAGCTTTTGCATATGGCTTACCCCCACTGGCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCa                                                                    <  2:234276/146‑1 (MQ=255)
                                                                              tCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTg                                                               >  2:103295/1‑150 (MQ=255)
                                                                                        ggAAGGTCGGGAGACTAATTTTGCTGCCCCAGTTGTTGAAGAGGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCg                                                     <  1:754038/151‑1 (MQ=255)
                                                                                        ggAAGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGccc                                                      <  2:722012/150‑1 (MQ=255)
                                                                                          aaGGTCAGGAGACTGATTTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCgg                                                    <  2:413149/150‑1 (MQ=255)
                                                                                               cAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcatcat                                              >  1:672934/1‑151 (MQ=255)
                                                                                                       cTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTtctc                                        >  2:821205/1‑150 (MQ=255)
                                                                                                        tGATTTTGCTGCCCCAGTTGTTCGAGAGGGCGAACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                         <  2:591097/148‑1 (MQ=255)
                                                                                                          attttGCTGACCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCa                                       <  1:1165636/146‑1 (MQ=255)
                                                                                                             ttGCGGCCCCAGTTGTTGCAGATGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAacac                                  <  2:1154849/150‑1 (MQ=255)
                                                                                                                cTGCCCCCGCTGTTGCAGATGGCGTCCCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCg                                <  1:1140090/149‑1 (MQ=255)
                                                                                                                        gCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTc                      >  1:200049/1‑151 (MQ=255)
                                                                                                                            ttGCAGAGGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGt                   <  2:1008708/150‑1 (MQ=255)
                                                                                                                              gCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGa                 >  1:767351/1‑150 (MQ=255)
                                                                                                                                  aTGGCGTACCCCCACCGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTg                  <  1:838959/145‑1 (MQ=255)
                                                                                                                                  aTGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGc            >  2:543503/1‑151 (MQ=255)
                                                                                                                                  aTGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGc            >  1:564950/1‑151 (MQ=255)
                                                                                                                                     gcctcaCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAg             <  1:59370/142‑1 (MQ=255)
                                                                                                                                      cGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCg        <  2:849315/151‑1 (MQ=255)
                                                                                                                                      cGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCg        >  1:208346/1‑151 (MQ=255)
                                                                                                                                      cGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCg        >  1:776917/1‑151 (MQ=255)
                                                                                                                                         acccccACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGt      <  2:943318/148‑1 (MQ=255)
                                                                                                                                           ccccccTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCt   <  1:980549/151‑1 (MQ=255)
                                                                                                                                            ccccCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTa  <  2:961545/151‑1 (MQ=255)
                                                                                                                                            cccACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTa  <  2:102699/151‑1 (MQ=255)
                                                                                                                                            cccACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCt   >  2:877762/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTCCTA  >  NC_000913/3350708‑3350996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: