Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	47.0%	76.7 / 35.2	66	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (30/5);  new base C (0/31);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 6.18e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443127‑3443427                                                                                                                                                           |                                                                                                                                                       gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                        <  2:308355/151‑1 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                       >  1:627013/1‑151 (MQ=255)  cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                       <  2:405382/151‑1 (MQ=255)        cATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                                 >  2:1056734/1‑151 (MQ=255)          tAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                                >  2:1057394/1‑150 (MQ=255)           agagTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCa                                                                                                                                               <  2:797836/150‑1 (MQ=255)               tAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTc                                                                                                                                          >  1:1172743/1‑151 (MQ=255)                          gCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                               >  2:407186/1‑151 (MQ=255)                            ggTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGt                                                                                                                              >  1:58789/1‑150 (MQ=255)                             gtttgccAGGCCTGAAAATACAGCAAATTTTTTGTCA‑CCCTTTCCAACCCA‑GTCCCCCCCCCGACCCATGACGCGATGG‑TCGCCGGAAAAAAAATAATACTGGAAGCAAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                             <  2:777584/146‑1 (MQ=255)                                 cccaagcctaaaaaaACAGCAAATTTTTTGTCAGCCATTCCCAA‑CAA‑TTCCCCCCCCCGACCCATGCCGCGATGG‑TCGCGGGGAACAAAATAATACGGGAAGCAAAGTTTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                         <  2:1109333/140‑1 (MQ=255)                                  cgggggcgacAAAGACAGGAAAATTTTTGGCAGCCTCTTCCAC‑CCA‑GTCCCGCCCCCAAACCATGGCGCTGTGG‑GCGCCGGAAACAGAATAATTCGGAAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                        <  2:928652/142‑1 (MQ=255)                                           cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                               >  2:238631/1‑151 (MQ=255)                                            aaaacaaGCAAATTTTTTTTCAGCC‑GTTCCCA‑CCAGGTCCCCCCCCAGAACCATGGCGGGGTGG‑TCCCGGGAAAAAAAATATTACGGGAGGCGAAGATGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  <  1:1079725/141‑1 (MQ=255)                                            aaaaACAGGAAATTTTTTGCCAGCCAGTCCCAC‑CCA‑GCCCCCCCCCCAAACCATGACGGGAGGG‑CCGCGGGAAAAAAAATAATCCGGGAAGCAAAGATTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                               <  2:326669/150‑1 (MQ=255)                                            aaaaACAGAGAAATTGTTGTGAGCCGGTCCAAG‑CTT‑GTCCCCCCCCCGACCGTGGTGGGGGTGG‑TCGCCGGGAACAAAAAAATCGGGGAGGAGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgtg                                                                                                              <  2:263998/151‑1 (MQ=255)                                                      aatttgttgTCAGCCTGTCCCAA‑CCA‑GTCCCCCCCCCAAACCATGACGCGATGG‑TCGCGGGAAAAAGAATAATACTGAAAGCAAAGATGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATa                                                                                                       <  2:950170/145‑1 (MQ=255)                                                      aaatggtgttaaGCCATTCCCAA‑CCA‑GCCCCCCCCCCAAACCATGACGCGATGG‑TCGCGGGAAACAAAATAATCCTGGAAGCAAATATTCCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                                                                            <  2:768334/132‑1 (MQ=255)                                                            ttgtCAGCCAGTCCCAC‑CCA‑GTCCCCCCCCCAACCCATGACGGGATGG‑TCGCGGGAAAAAAAAAAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  2:609476/150‑1 (MQ=255)                                                            ttgcCACCCAGTCCCAC‑CCG‑GTCCCCCCCCCAACCCATGACGGGAGGG‑TCGCCGGAAAAAGAAAAATACTGAAAGAGAAGATGGCGGGAATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                               <  2:190501/150‑1 (MQ=255)                                                             tgtCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                              >  2:616021/1‑150 (MQ=255)                                                              gtCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                            >  2:374020/1‑151 (MQ=255)                                                               tCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                           >  1:1183882/1‑151 (MQ=255)                                                                   ccAGTTCCAC‑CCA‑GCCCCCCCCCCGAACCATGACGCGATGG‑TCGCGGGAAAAAAAAAAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                        <  2:844429/150‑1 (MQ=255)                                                                   ccAGTCCCAA‑CCA‑GCCCCCCCCCAGAACCAAGCCGCGATGG‑TCGCCGGAAAAAGAATAATACTGGAGGCGAAGATTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCtt                                                                                         <  1:119333/149‑1 (MQ=255)                                                                       tcccAC‑CCA‑GTCCCCCCCCAGAACCATGCCGGGATGG‑CCGCCGGAAAAAAAATAATACGGAAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                                                                    <  2:390331/148‑1 (MQ=255)                                                                        ttcccacccgccaCCCCCCCCGATCCCTGCCGCAATGG‑TCGCCCGGCACAAAAAAATACTGGAAGCAAGGTTTGCGGGACTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCt                                                                                      <  2:214440/137‑1 (MQ=255)                                                                        tCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGa                                                                                    >  1:573859/1‑149 (MQ=255)                                                                         ccAC‑CCA‑GTCCCGCCCCCGACCCAGGACGCGATGG‑TCGCGGGGAACAGAATAATACTGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                     <  2:345867/147‑1 (MQ=255)                                                                               caagccccccccccAAACCATGACGCGATGG‑TCGCCGGAAAAAAAAAAATGTAGGAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              <  2:58789/141‑1 (MQ=255)                                                                                a‑GTCCCCCCCCCGAACCAGGACGGGATGG‑TCGCCGGGAAAAAAATAATTCTGGAAGCAAATATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                            <  1:450799/150‑1 (MQ=255)                                                                                     ccccccccGACCCAGGACGCGATGG‑TCGCGGGAAAAAAAAAAATACTGGAAGCAAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       <  1:513040/148‑1 (MQ=255)                                                                                     ccccccccAACCCAGGCCGGGAGGG‑TCGCCGGAAAAAAAATAATACTGGAAGAGAAGTTTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       <  1:215920/148‑1 (MQ=255)                                                                                     ccGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                       >  1:1012519/1‑151 (MQ=255)                                                                                      cccccccAAACCATG‑CGGGATGGTTCGCCGGAAAAAAAAAAATAGGGAAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTa                                                                         <  1:804002/146‑1 (MQ=255)                                                                                      cGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                      >  2:1145350/1‑151 (MQ=255)                                                                                       ccccccAAACCATGCCGGGATGG‑CCCCGGGAAAAAAAAAAATACTGGAAGCGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     <  1:141043/150‑1 (MQ=255)                                                                                         ccccGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                                   >  1:985850/1‑151 (MQ=255)                                                                                               aCCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             >  1:958185/1‑151 (MQ=255)                                                                                                   tGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcgg                                                         >  2:570148/1‑151 (MQ=255)                                                                                                       gcgtggg‑TCGCCGGAAAAAAAATAATACGGAAAGCAAAGATTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                           <  1:916702/140‑1 (MQ=255)                                                                                                                cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                             >  1:1026855/1‑151 (MQ=255)                                                                                                                   cGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                           >  1:1108740/1‑150 (MQ=255)                                                                                                                    ggaaaaaaaataatACTGAAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaa                                         <  1:681304/143‑1 (MQ=255)                                                                                                                          aaaataatACGGAAAGCAAAGATGGCCGGGATCACCCCCGCCATATTCACTTTCAGCGGTAAATGTGAGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  1:1067079/149‑1 (MQ=255)                                                                                                                          aaaaaaatcCTGGAAGGGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    <  1:379788/145‑1 (MQ=255)                                                                                                                          aaaaaaatcCTGAAAGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                                   <  2:783025/146‑1 (MQ=255)                                                                                                                          aaaaaaataCGGGAAGCAAAGATTGCCGGGTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                        <  1:381577/141‑1 (MQ=255)                                                                                                                          aGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    >  1:132551/1‑150 (MQ=255)                                                                                                                            aataatACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                                  >  1:369705/1‑150 (MQ=255)                                                                                                                               aataCTGGAAGGGAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              <  2:1150077/151‑1 (MQ=255)                                                                                                                                ataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                              >  1:1028364/1‑150 (MQ=255)                                                                                                                                  acgggAAGCAAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                            <  1:673048/147‑1 (MQ=255)                                                                                                                                  aCTGAAAGCGAATATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                            <  1:856876/150‑1 (MQ=255)                                                                                                                                   cTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                             >  1:1153547/1‑148 (MQ=255)                                                                                                                                        aGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     >  2:1114661/1‑151 (MQ=255)                                                                                                                                         gagaatgttGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                        <  1:396243/140‑1 (MQ=255)                                                                                                                                         gCGAAGATGGCGGGTATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     <  1:1171629/150‑1 (MQ=255)                                                                                                                                           gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  >  1:531463/1‑151 (MQ=255)                                                                                                                                           gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  >  2:189926/1‑151 (MQ=255)                                                                                                                                              gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc                >  2:258484/1‑150 (MQ=255)                                                                                                                                                 tGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              >  1:1137770/1‑149 (MQ=255)                                                                                                                                                   cggggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaa              <  2:419736/145‑1 (MQ=255)                                                                                                                                                   ccGGTATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa               <  2:449831/146‑1 (MQ=255)                                                                                                                                                    cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata           >  2:502166/1‑149 (MQ=255)                                                                                                                                                     gggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:143285/1‑150 (MQ=255)                                                                                                                                                        aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat      >  1:309486/1‑150 (MQ=255)                                                                                                                                                           aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  1:408241/1‑151 (MQ=255)                                                                                                                                                           |                                                                                                                                                       GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGG‑CTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAA‑CCA‑GTACCGCCCCCGAACCATGACGCGATGG‑TCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443127‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑