Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,986	A→C	N34K (AAT→AAG)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,986	0	A	C	100.0%	343.6 / NA	84	N34K (AAT→AAG)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (39/45);  total (39/45)

AGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAT  >  NC_000913/3400838‑3401127                                                                                                                                                     |                                                                                                                                              agTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCtt                                                                                                                                             <  2:292704/151‑1 (MQ=255)  gTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTc                                                                                                                                            >  2:925139/1‑151 (MQ=255)     ggCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAAt                                                                                                                                         <  2:184050/151‑1 (MQ=255)       cGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATAc                                                                                                                                       <  2:1183444/151‑1 (MQ=255)           aaCGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATg                                                                                                                                   >  1:632481/1‑151 (MQ=255)              gCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCt                                                                                                                                <  2:1141575/151‑1 (MQ=255)                cGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTg                                                                                                                              <  2:995099/151‑1 (MQ=255)                      tCATTGGGGCAATGGGAGCCAAATTGCCCGGGGGACCGGCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCtt                                                                                                                         <  1:692533/150‑1 (MQ=255)                      tCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCtt                                                                                                                         <  2:704929/150‑1 (MQ=255)                        aTTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCtttt                                                                                                                       >  1:376271/1‑150 (MQ=255)                        aTTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTa                                                                                                                      <  2:796487/151‑1 (MQ=255)                         ttGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTAc                                                                                                                     >  2:983004/1‑151 (MQ=255)                           gggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACa                                                                                                                    <  2:74758/150‑1 (MQ=255)                           gggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACa                                                                                                                    <  2:726913/150‑1 (MQ=255)                            ggCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATa                                                                                                                  <  1:635708/151‑1 (MQ=255)                             gCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATa                                                                                                                  >  2:143958/1‑150 (MQ=255)                               gAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAAt                                                                                                               >  1:894342/1‑151 (MQ=255)                               gAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAAt                                                                                                               >  2:1077200/1‑151 (MQ=255)                               gAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAAt                                                                                                               <  1:1066250/151‑1 (MQ=255)                               gAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAAt                                                                                                               <  1:270689/151‑1 (MQ=255)                                aaTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATg                                                                                                              >  1:310247/1‑151 (MQ=255)                                aaTGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATAGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATg                                                                                                              >  2:262763/1‑151 (MQ=255)                                   ggCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAg                                                                                                            <  2:560790/150‑1 (MQ=255)                                    gcagcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAggg                                                                                                          >  1:719539/1‑151 (MQ=255)                                       gcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAt                                                                                                       >  2:385006/1‑151 (MQ=255)                                       gcaATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAt                                                                                                       <  1:1046406/151‑1 (MQ=255)                                        caATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTAtt                                                                                                      <  2:982623/151‑1 (MQ=255)                                          atatTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCg                                                                                                    <  2:340941/151‑1 (MQ=255)                                                  cGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAccc                                                                                            <  2:1044903/151‑1 (MQ=255)                                                    gCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTAccc                                                                                            >  2:257609/1‑149 (MQ=255)                                                     cGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAgg                                                                                         >  2:739933/1‑151 (MQ=255)                                                      gTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                                                                        <  1:1060114/151‑1 (MQ=255)                                                      gTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGt                                                                                        <  1:1113104/151‑1 (MQ=255)                                                        aCGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTc                                                                                       >  2:82607/1‑150 (MQ=255)                                                         cGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCa                                                                                      >  1:620070/1‑150 (MQ=255)                                                         cGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCa                                                                                      >  1:899174/1‑150 (MQ=255)                                                             ccAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGa                                                                                 <  2:418866/151‑1 (MQ=255)                                                               aGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                                                                                >  1:529725/1‑150 (MQ=255)                                                               aGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGAc                                                                                >  2:347868/1‑150 (MQ=255)                                                                     tGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAt                                                                         >  1:19622/1‑151 (MQ=255)                                                                     tGCCTCGCGTCAAGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGACTGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAt                                                                         >  1:725545/1‑151 (MQ=255)                                                                      gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGt                                                                            <  2:213912/147‑1 (MQ=255)                                                                      gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTc                                                                           <  1:445495/148‑1 (MQ=255)                                                                      gCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCAtt                                                                        >  2:184500/1‑151 (MQ=255)                                                                       cTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTg                                                                       >  2:1199733/1‑151 (MQ=255)                                                                       cTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTg                                                                       >  2:880749/1‑151 (MQ=255)                                                                         tCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTgg                                                                      <  2:973156/150‑1 (MQ=255)                                                                          cgcgTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGa                                                                     >  1:874290/1‑150 (MQ=255)                                                                           gcgTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGaaa                                                                   <  1:1058078/151‑1 (MQ=255)                                                                            cgTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGaaa                                                                   >  1:169986/1‑150 (MQ=255)                                                                             gTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAAc                                                                 >  2:19628/1‑151 (MQ=255)                                                                             gTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAAc                                                                 <  2:967806/151‑1 (MQ=255)                                                                              tCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAAc                                                                 >  2:278286/1‑150 (MQ=255)                                                                               cATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACAt                                                               <  1:446043/151‑1 (MQ=255)                                                                                aTGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATg                                                              >  2:949941/1‑151 (MQ=255)                                                                                          gCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtt                                                    >  1:696015/1‑151 (MQ=255)                                                                                           cAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAttt                                                   <  2:571438/151‑1 (MQ=255)                                                                                           cAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGGCATTGGAAAACATGCCACGAAAttt                                                   <  1:113873/151‑1 (MQ=255)                                                                                            aGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtttt                                                  >  2:960661/1‑151 (MQ=255)                                                                                                aCGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAAtttttt                                                <  2:756455/149‑1 (MQ=255)                                                                                                aCGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTc                                               >  1:831676/1‑150 (MQ=255)                                                                                                  gCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAAc                                            >  1:874373/1‑151 (MQ=255)                                                                                                     tttCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACa                                           <  1:932914/149‑1 (MQ=255)                                                                                                     tttCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACa                                           <  1:980502/149‑1 (MQ=255)                                                                                                           tGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAgg                                   >  2:735229/1‑151 (MQ=255)                                                                                                              agtggCAGGATTCTGAAGAATGGACAGGACGGAAGGATCCTTCAATACGATGCCTTGTCATTTTAAATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGACAAGAAATTTTTTCAACATACTAAGGGAt                                >  1:80952/4‑151 (MQ=255)                                                                                                                 ggCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt                             <  2:561292/151‑1 (MQ=255)                                                                                                                 ggCACGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAAt                             <  2:241090/151‑1 (MQ=255)                                                                                                                    aCGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt                          <  2:791436/151‑1 (MQ=255)                                                                                                                    aCGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt                          <  1:339334/151‑1 (MQ=255)                                                                                                                     cGATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCt                          <  1:747255/150‑1 (MQ=255)                                                                                                                      gATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTg                         >  1:340858/1‑150 (MQ=255)                                                                                                                      gATCCTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGa                        <  1:143956/151‑1 (MQ=255)                                                                                                                         ccTGACGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGaaa                      <  1:464574/150‑1 (MQ=255)                                                                                                                             aCGAATGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTgg                 <  2:784289/151‑1 (MQ=255)                                                                                                                                aaTGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGc              <  2:238266/151‑1 (MQ=255)                                                                                                                                 aTGGCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCg             >  1:724530/1‑151 (MQ=255)                                                                                                                                   ggCCACCACGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGga           <  2:148840/151‑1 (MQ=255)                                                                                                                                     ccaccaCGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaa          >  2:737278/1‑150 (MQ=255)                                                                                                                                      caccacGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaa        <  1:164162/151‑1 (MQ=255)                                                                                                                                       accacGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaag       <  1:382213/151‑1 (MQ=255)                                                                                                                                          acGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa    <  1:881250/151‑1 (MQ=255)                                                                                                                                           cGGAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGgaaaagaaa    >  2:525513/1‑150 (MQ=255)                                                                                                                                            ggAAGGCTCCTTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAt  >  2:432012/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                              AGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCTGCTTCGCGTCATGACCTACTGCAGCTACGCTTTTCGGTGAACCGGCACGATCCTGACGAATGGCCACCACGGAAGGCTCATTCAATACGATGCCTTGTCCTTTTACATAAATGAGGGTATTCGCAGTACCCAGGTCAATGGACAAGTCATTGGAAAACATGCCACGAAATTTTTTCAACATACTAAGGGATAATCCTGAAAGCTGGGGCGGAAAAGAAAAT  >  NC_000913/3400838‑3401127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑