Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	907,602	0	A	C	44.6%	77.3 / 22.0	56	G227G (GGT→GGG)	ybjT	putative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (28/3);  new base C (0/25);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGC  >  NC_000913/907457‑907750                                                                                                                                                   |                                                                                                                                                     gcgcGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGc                                                                                                                                                  >  2:565139/1‑150 (MQ=255)    cgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCAc                                                                                                                                              >  2:464998/1‑151 (MQ=255)        atcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGcc                                                                                                                                          >  2:692118/1‑151 (MQ=255)              cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATaaa                                                                                                                                     >  2:484383/1‑150 (MQ=255)              cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATaa                                                                                                                                      >  1:714393/1‑149 (MQ=255)                    cagATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTc                                                                                                                               >  1:526317/1‑150 (MQ=255)                     agATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCa                                                                                                                              >  1:218707/1‑150 (MQ=255)                       aTCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAAc                                                                                                                           >  2:1065445/1‑151 (MQ=255)                       aTCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAAc                                                                                                                           >  2:1030485/1‑151 (MQ=255)                        tCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACt                                                                                                                          >  1:439353/1‑151 (MQ=255)                         cGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTg                                                                                                                         >  2:230153/1‑151 (MQ=255)                               tCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTCCGGAAGTAATCCCATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCt                                                                                                                    >  1:731486/1‑150 (MQ=255)                               tCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGACACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                                                   >  2:905295/1‑151 (MQ=255)                                cAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                                                   >  1:843317/1‑150 (MQ=255)                                cAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                                                   >  1:787714/1‑150 (MQ=255)                                   ccccTAAATCACCGCCCGGGGGGGGGG‑GGGGGGTACGGAAGTAATCACTTTGAAAACCCACCCCAAAACCCGGGGGGGGGGGGGGGGGAGGGGGATCACCCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGa                                                                                                                  <  2:810481/148‑1 (MQ=255)                                   cccataaacaaccccccGGGGGGTGGG‑GGGGGGTAGGGAAGAAACCACATTGAGAACCCCCACCAAAACCCGGGGGGGGGGGGGGGGGGGGGGAACCACCCGGGGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGAt                                                                                                                 <  1:567494/135‑1 (MQ=255)                                         aaTCACCGCCCGGGGGGGGGT‑GGGGGGTAGGGAATAAATCAGATTGAAAACCCACACCAAACTCCAGGGGGGGGGGGGGGGGATGGGGATCACCCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACt                                                                                                             <  1:177682/147‑1 (MQ=255)                                          aTCACCCCCCGGGGGGGGGG‑GGGGGGGAGGGAAGAAATCACATTGAAAACCCCCCCCAAAAGCCGGCGGGGGGGGAGGGGGAGGGGGATCAACCAGCGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCa                                                                                                        <  1:683168/151‑1 (MQ=255)                                          aTCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc                                                                                                         >  1:268388/1‑150 (MQ=255)                                             accccccGGGCGGTGGG‑GGGGGGAAGGGAAGTAATCACAGTAAAAAACCCCCCAAAAACCCAGGGGGGGGGGAGGGGGATGGGAATCAACCGGCGGCTCTTCCCGCTAACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAcc                                                                                                      <  2:851892/146‑1 (MQ=255)                                                  ccttggggtggtgGGGAGGTGCGGAACTAATCACTTAGAAAAGCCACCCGAAAATCCGGGGGGGGGGGGGTGGGATGGGGTTCCACCAGAGGTGAATACGACTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACct                                                                                                     <  1:1013770/141‑1 (MQ=255)                                                    agacggcggT‑GGGCGGCACGGAAGTAATCCCCTTGAGCAACCACACAGAAATCCAGCGGGAGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTc                                                                                               >  1:707521/4‑150 (MQ=255)                                                     ggCGGTGGG‑GGGCGGTACGAAAGTAACCACATTAAAAACCCCCCCCGAAACCCGGGGGGGGGGGGGGGGTGGGGGGATCACCGAGCGGCGGTCCCCCCTCACCGCCATAAAATGTTCAAACTGTCGCTGATAACTGAGCACCTCTGGTCcg                                                                                             <  1:932216/151‑1 (MQ=255)                                                     ggCGGGGGG‑GGGCGGTAGGGAAAAAATCACATGAAAAACCCCCCCCAAAACCCAGGGGGGGGGGGGGGGGATGGGAACCAACCGGGGGCCTTCCCCGCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTc                                                                                               <  1:366675/149‑1 (MQ=255)                                                      ggggtggg‑GGGGGGTAGGGAAGAAATCACATTGAAAACCCCCCCCAAAACCCGGGGGGGGGGGGGGGGGGGGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcgg                                                                                            <  2:269245/149‑1 (MQ=255)                                                             t‑ggGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTc                                                                                     >  2:1061866/1‑151 (MQ=255)                                                              gggggggtggggAAGAAATCACATGGAAAACCCCCCCCAAACCCCGGGGGGTGGGGGGGGGGAGGGGGCACGACCGGCGGCGCTCCCCGCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTc                                                                                     <  1:365632/141‑1 (MQ=255)                                                               gggCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCg                                                                                    >  2:670742/1‑151 (MQ=255)                                                                   ggTAGGAAATTAATCACTTGGAAAACCCCCCCCAAACCCCAGGGGGGGGGGGGGGGGATGGGGATCAACCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGa                                                                                <  2:478188/151‑1 (MQ=255)                                                                       cGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                                                                            >  1:218016/1‑151 (MQ=255)                                                                         gAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgt                                                                          >  2:730581/1‑151 (MQ=255)                                                                          aaGTAATCACATTGAAAACCCACCCCAAAACCCAGGGGGGGGGGGGGGGGAGGGGGATCACCCGGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgt                                                                          <  2:749234/150‑1 (MQ=255)                                                                           aGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgtgt                                                                        >  2:880169/1‑151 (MQ=255)                                                                                    atagaaaacccACACCGAAACCCAGGGGGGGGGGGGGGGGGGGGGAATCACCCAGGGGCGTTTCCCCCTCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGc                                                                <  2:28521/141‑1 (MQ=255)                                                                                        agaAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgat                                                           >  1:911613/1‑151 (MQ=255)                                                                                                 acCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaac                                                   >  1:730089/1‑150 (MQ=255)                                                                                                   cGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaacaac                                                >  2:451644/1‑151 (MQ=255)                                                                                                     aaaCCCAGGGGGGGGGGGGGGGAAGGGGGAAAACCCAGGGCCCCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaaca                                                  <  1:358295/147‑1 (MQ=255)                                                                                                      aacccgggggggggggggggggAGGGGAATCACCCGGGGGCCCTCCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCa                                             <  1:1030451/143‑1 (MQ=255)                                                                                                         ccAGGGGGGGGGGGGGGGGAGGGGGATCACCCAGGGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCaa                                            <  2:1102954/149‑1 (MQ=255)                                                                                                          cggCGGGGGGGGGGGGGGAGGGGAACCACCCGGCGGCGCTTCCCCCCCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCa                                         <  1:230142/149‑1 (MQ=255)                                                                                                             gggggggggggggggAGGGGGATCACCCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGAt                                      <  2:332998/150‑1 (MQ=255)                                                                                                             gggggggggggggggAGGGGGACCAACCAGGGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGAt                                      <  1:381995/150‑1 (MQ=255)                                                                                                              ggggggggggggggAGGGGAACCACCCGGGGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGAt                                      <  2:704446/150‑1 (MQ=255)                                                                                                                        ggggagggggatCACCCAGCGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAg                           <  2:456737/151‑1 (MQ=255)                                                                                                                          gggtggggatCACCCAGCGGCGCTCCCCGCTCTCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGtt                         <  1:573149/148‑1 (MQ=255)                                                                                                                             tggggatCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTc                      >  2:899466/1‑151 (MQ=255)                                                                                                                             ggggaatCACCCGGCGGCGCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTc                      <  1:664365/150‑1 (MQ=255)                                                                                                                               ggaaacacccAGCGGCCTTTCCCGTTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCa                    <  2:881120/143‑1 (MQ=255)                                                                                                                                     aaCCGGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGGGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAgc                  <  2:294897/147‑1 (MQ=255)                                                                                                                                        cAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAAt             <  2:445006/149‑1 (MQ=255)                                                                                                                                          gcggcgCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATgg           <  2:827218/149‑1 (MQ=255)                                                                                                                                            ggcgCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGc         <  2:87062/149‑1 (MQ=255)                                                                                                                                                cTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGCGCAGCAAGTTTTCCAGCGCAATGGGCgtggtg   >  1:502238/1‑151 (MQ=255)                                                                                                                                                 ttACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGc  >  2:524104/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGT‑GGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCAATGGGCGTGGTGC  >  NC_000913/907457‑907750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑