| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 941,713 | 0 | T | G | 50.0% | 71.2 / 25.5 | 58 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
| Reads supporting (aligned to +/- strand): ref base T (4/25); new base G (29/0); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.72e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT > NC_000913/941572‑941862 | cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACt < 1:209291/150‑1 (MQ=255)cctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACt < 1:1080486/150‑1 (MQ=255) ctccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTa > 1:520318/1‑150 (MQ=255) tccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTAt > 2:128638/1‑150 (MQ=255) ccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATc < 1:1112710/150‑1 (MQ=255) gcgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTg < 1:1101622/151‑1 (MQ=255) gcgcAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCttt > 1:61003/1‑150 (MQ=255) cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGAcct > 2:1095715/1‑137 (MQ=255) cAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGCGGGGGGACTTACTATCTTTaa > 2:424234/1‑150 (MQ=255) aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCCTACTATCTTGAAAAgg > 2:375338/1‑151 (MQ=255) gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCa < 2:956880/150‑1 (MQ=255) aaCTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTTTCTTTAAAACGGCCGCCAGAAAACt > 1:786131/1‑150 (MQ=255) cTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATCT‑aa > 2:277399/1‑150 (MQ=255) tataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAAGCACGCCCGAAAACT‑aa > 2:680412/1‑149 (MQ=255) aCGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCAt < 2:344968/151‑1 (MQ=255) cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCGCGCCAGAAATCT‑AATGCCCGCa < 1:759506/149‑1 (MQ=255) cggcggCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATg < 2:360448/151‑1 (MQ=255) cggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTTATATCTTTAACAGGCACGCCCGAAATCT‑TATGCCCGCCTGa > 1:887452/1‑149 (MQ=255) cAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGa < 2:616487/151‑1 (MQ=255) aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGAt < 2:561444/151‑1 (MQ=255) aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACGCCAGAAAACT‑AATGCCCGCATGatcat > 2:932779/1‑144 (MQ=255) aaCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTTGGGGGAATTACTATCTTTAAAAGGGCCGCCCGAAAACT‑AATGCCCCCCTGATAAACATCCATCcgccc > 2:593949/1‑148 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCtata < 1:997407/151‑1 (MQ=255) aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTTTTATCTTTAACAGGCACGCCCGAAAACT‑AATGCCCCCACAATTATCATATATcc > 1:347110/1‑143 (MQ=255) ccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAAGCCCGCCAGAAATCT‑AATGCCCCCCTGATCATCATCGATCCGCGCTATAcc > 2:410356/1‑150 (MQ=255) cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAAACT‑AATGCCCGCATGATCATCATCGATCCGCGCTATAcc > 1:416565/1‑149 (MQ=255) gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGac < 1:1095677/151‑1 (MQ=255) gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAAAAGGCCCCCCAGAAATCT‑AATGCCCGCCAGAACATCATCGATCCGCGCTCTAcc > 1:131737/1‑148 (MQ=255) gATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCAGAAAACT‑AATGCCCCCCTGATCATCATCGATCCGCGCTATACCGCCAcc > 2:67008/1‑150 (MQ=255) atatCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAAGCACGCCCGAAATCT‑TATGCCCCCATGATCATCATCGAACCGCGCTAAACCGACAcc > 2:969222/1‑149 (MQ=255) cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACCCCAGAAAACT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGcc > 2:56005/1‑151 (MQ=255) aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCgg < 2:1051480/151‑1 (MQ=255) aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACGTTCTCTATTTGACAAGGACGCCACAAATCTAAATGCCC‑CCTGATCATCACAGAACCCCGCTATACACGCACCGGGGCCg > 2:866271/1‑150 (MQ=255) aaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCggg < 1:149004/151‑1 (MQ=255) aCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcg < 2:719979/151‑1 (MQ=255) gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAAGCACGCCAGAAAACT‑AATGCCCGCCTGATCATCATCGATCCCCCCCATACCGACACccgggccggg > 2:394836/1‑146 (MQ=255) aGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTGAAAAGGCACGCCAGAAAAAT‑AATGCCCCCCTGATCATCATCGATCCCCGCTATACCGAAACCCGGggcgggcggg > 1:996/1‑145 (MQ=255) gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGAt < 2:422934/151‑1 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATg < 2:710427/149‑1 (MQ=255) ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAg < 2:862102/151‑1 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTg < 1:313994/150‑1 (MQ=255) aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTTAAAAAGCACGCCAGAAAACT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGCCACCGGTGCCGGGGGCGAAGATGAggg > 1:615984/1‑148 (MQ=255) gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATcc < 1:1061425/149‑1 (MQ=255) gTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTTTTTTGAACAGGCACGCCACAAATCT‑AATGGCCGCATCATCATCATCCAGACGCGCTATAACAACACCGGTGGCCGGGGCGAAGATGAGAGGGTccc > 2:654838/1‑150 (MQ=255) tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCCCGCCCGAAATCT‑AATTCCCGCATGATCATCATCGATCCCCGCTATACCGACACCCGGGCCGGGCGCGAAGATGAGTGGGTccc > 2:1141467/1‑149 (MQ=255) taaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATTTTTAAAAAGCACGCCAGAAATCT‑AATGCCCGCACGATCATCATCGATCCGCCCTATACCGACACCGGTGCCGGGCGCGGAGATGAGTGGATCCCCATTCGTcc > 1:806008/1‑150 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTTGGGGGACATGCTATTTTTAAAAAGCACGCCACAAAACT‑TATACCCTCCTAGTTATTATCCAACCGCGCCCTAACGACACCGGTGCCGGGGCCGGAGATGCGCGGGTACCTATTCTTCCgg > 1:1048091/1‑151 (MQ=255) cccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCCTAATATCTTTAACAGGCACGCCAGAAAACT‑AATGGCCGCCTGATCATCATCGATCCGCGCTATACCGCCCCCCGTGCCCGGGGCGGAGATGAGGGGGACCCCTTTCGTCCggg > 1:419255/1‑150 (MQ=255) cgcgAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGc < 1:1005268/151‑1 (MQ=255) aaTGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTg < 2:641611/151‑1 (MQ=255) aaTGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTg < 2:103893/151‑1 (MQ=255) aTGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAATCT‑AATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGGAGATGAGGGGATCCCTATTCGTCCGGGGAAAGATGCCGCACTgg > 2:1164044/1‑151 (MQ=255) tGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc > 1:117844/1‑151 (MQ=255) ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg < 1:1012814/151‑1 (MQ=255) ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg > 1:59877/1‑151 (MQ=255) gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCt > 2:1157259/1‑151 (MQ=255) ggggTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTgg > 2:1088267/1‑151 (MQ=255) gTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt < 2:226765/151‑1 (MQ=255) tGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt > 1:676784/1‑150 (MQ=255) | CCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCT‑AATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT > NC_000913/941572‑941862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |