Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	42.5%	45.7 / 30.1	47	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (23/4);  new base A (0/20);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.27e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTG  >  NC_000913/1222448‑1222737                                                                                                                                                        |                                                                                                                                               cAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGa                                                                                                                                               >  1:58440/1‑150 (MQ=255)       tGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAaaacaa                                                                                                                                         >  1:693428/1‑150 (MQ=255)         gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAaaacaaac                                                                                                                                       >  1:239918/1‑150 (MQ=255)          cattacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAaaacaaac                                                                                                                                       >  2:17025/1‑149 (MQ=255)           attacccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAgg                                                                                                                                    <  1:812275/151‑1 (MQ=255)              acccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑cc                                                                                                                                 <  2:548480/150‑1 (MQ=255)               cccattaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCg                                                                                                                               <  2:1020849/151‑1 (MQ=255)                      ccGCAAAAATTTCTGCACCCTGTGCGCTGTCAGTTTTTGCCATTTGGTTGGTTCCCGCGCAATTAATATAGGTTTCTGAGGCCAAACCT‑TTTTAAATATTGTAGCCCGGCGCCCGGGTCATT‑GG‑TGCAAAAAAAAACGGG‑CCCGCCAGTa                                                                                                                         <  1:1126430/150‑1 (MQ=255)                            caaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCaa                                                                                                                   >  2:568604/1‑150 (MQ=255)                                      acccTGTGCGCTGTCAGTTTTTGCCATTTCGTTGTTTTCTGCGAAATTAATATAGTTTCCGGGCGGCAACCCT‑TTTTTAATATTGTAGCCCTGCCCCCAGGTCTTT‑GG‑TGAAAAAAAAAAAGGG‑CCCGCCAGTAAAGCAAAtttttt                                                                                                            <  2:394063/145‑1 (MQ=255)                                      aaCCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTtcat                                                                                                         >  1:473734/1‑150 (MQ=255)                                        ccTGTGCGCTGTCATTTTTTGCCTTTTCGTTGGTTTCTGCGAAAGAAATATAGGTTTCTGACGGAAAACCT‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTtcatca                                                                                                       <  2:189182/150‑1 (MQ=255)                                           gtgCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTcatcatcat                                                                                                   >  1:600059/1‑151 (MQ=255)                                              cgctttaattttttGCCTTTTCGTTGGTTTCTGCGAAAGAAATATAGGTTTCTGACGGAAACCCT‑TTTTTAATTTTGTAGTCCTGCGCCCAGGTCTTT‑GG‑TGCAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCAtt                                                                                                  <  2:836146/140‑1 (MQ=255)                                              cgcTGTCATTTTTTGCCTTTTCGTTGTTTTCTGCGAAAGTAATATAGGTTTCGGGCGGCAAACCG‑TTTTAAATATTGTAGCCCGGCGCCCAGGTCTTG‑GG‑TCCAAAAACAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTcc                                                                                                <  2:1028499/151‑1 (MQ=255)                                              cgcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTcc                                                                                                >  2:81727/1‑151 (MQ=255)                                               gcTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCt                                                                                               >  1:881985/1‑151 (MQ=255)                                                       tttttGCCTTTTCGTTGTTTTCTGCGCAAGTAATATAGTTTTCTGACGGCAACCCT‑TTTTAAATATTGTAGTCCTGCCCCCAGGTCATT‑GG‑TGCAAAAACAACCAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAttt                                                                                          <  2:630910/148‑1 (MQ=255)                                                          ttGCCTTTTCGTTGGTTTCTGCGAAAGTAATATAGTTTTCTGACGGCAACCCT‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCAAAAAAAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAttt                                                                                     <  2:561584/150‑1 (MQ=255)                                                           tcccttttCGTTGGTTTCTGCGAAATTAATATAGGTTTCTGAGGACAACCCT‑TTTTAAATATGGTAGCCCGGCGCCC‑GGTTTTT‑GGTTGGAAAAACAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                    <  2:332837/145‑1 (MQ=255)                                                            gCCTTTTCGTTGGTTTCGGCGAAAGTAATATAGGTTTCTGACGGCAACCCT‑TTTTAAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                    <  2:434084/149‑1 (MQ=255)                                                                tttGGTTGTTTTCTGCGAAAGAAATATGGTTTTCTGCCGAAAACCTT‑TTTTTAATTTTGTAGTCCTGCGCCCAGTTTTTT‑GG‑TGCAAAAACAAAAAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                               <  1:563815/150‑1 (MQ=255)                                                                   cGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAt                                                                           >  2:582548/1‑151 (MQ=255)                                                                     ttGGCTTCTGCGAAAGTAATATAGGTTTCTGACGGCAACCCT‑TTTTTAATATTGTAGTCCGGCCCCCAGGTCATT‑GG‑GGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAg                                                                              <  2:585654/146‑1 (MQ=255)                                                                     ttGCCTTCTGCGAAAGAAAATTAGGTTTCTGACGGAAAACCT‑TTTTTAATATGGTCGCCCGGCGCCCATTTCTTT‑GG‑TGCAAAACCAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                          <  1:908196/150‑1 (MQ=255)                                                                       ggtttCTGCGAAAGAAATTTAGGTTTCTGACGGAAACCCT‑TTTTAAATATTATGGCCCTGCACCCGGTTCTTT‑GG‑TGTGAAAAAAAACACC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                        <  1:1061199/147‑1 (MQ=255)                                                                         cTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                     >  1:154396/1‑151 (MQ=255)                                                                         cTTCTGCGCAAGTAATATAGGCTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcacc                                                                     >  1:776534/1‑151 (MQ=255)                                                                          ttCTGCGCAAATAATATAGTTTTCTGCCGGCAACCTT‑TTTTTAATATTGTACCCCGCCCCCCGGGTATTT‑GG‑TGCAAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGc                                                                        <  1:673439/147‑1 (MQ=255)                                                                          ttCTGCGAAAGTAATATAGTTTTCTGCCGGCAACCTT‑TTTTTAATATTGTAGTCCTGCGCCCAGGTTATT‑GG‑TGCAAAAAAAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcac                                                                      <  2:39737/149‑1 (MQ=255)                                                                             tGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCAccc                                                                 >  1:581823/1‑151 (MQ=255)                                                                                gcAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                >  2:931827/1‑149 (MQ=255)                                                                                      aaTATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGcc                                                         >  1:768068/1‑150 (MQ=255)                                                                                         ataGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                     >  1:660688/1‑151 (MQ=255)                                                                                                    gccgCAAACCCTTTTTTAATTTTGTGTCCCTCCCCCCAGTTCTTT‑GG‑T‑CAAAAAAAAACCGGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCt                                            <  1:212936/147‑1 (MQ=255)                                                                                                    aCGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                          >  2:562216/1‑151 (MQ=255)                                                                                                                tttttAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCtt                               >  1:745386/1‑151 (MQ=255)                                                                                                                ttttaAATTTTTTAGCCCTGCGCCCAGTTCATT‑GG‑CGAAAAAACAAACGCC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCt                                <  1:1014425/150‑1 (MQ=255)                                                                                                                       ttttGTAGCCCTGCCCCCAGGTATTG‑GG‑GGCAAAAAAAAACGGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGc                         <  2:1072829/148‑1 (MQ=255)                                                                                                                           tgtatccccgccccAGGTTCTTGGG‑TGCAAAAAAAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCAt                      <  2:613297/139‑1 (MQ=255)                                                                                                                                ccTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGt               >  2:642059/1‑151 (MQ=255)                                                                                                                                ccTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGt               >  2:754861/1‑151 (MQ=255)                                                                                                                                  tGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTc              >  1:1139889/1‑150 (MQ=255)                                                                                                                                         aGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGcc      >  2:588448/1‑151 (MQ=255)                                                                                                                                            ttttt‑GG‑TGAAAAAACAAACGGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCa     <  1:1094776/146‑1 (MQ=255)                                                                                                                                             cATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg  >  2:983794/1‑151 (MQ=255)                                                                                                                                              ttt‑GG‑TGAAAAAAAAAACAGC‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAg    <  1:396377/147‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                               CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCG‑TTTTTAATATTGTAGTCCTGCGCCCAGGTCATT‑GG‑TGCGAAAACAAACAGG‑CCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTG  >  NC_000913/1222448‑1222737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑