Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	43.8%	104.5 / 49.3	73	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (30/11);  new base A (0/32);  total (30/43)
Fisher's exact test for biased strand distribution p-value = 1.91e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTG  >  NC_000913/29338‑29630                                                                                                                                                       |                                                                                                                                                 tttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCcaca                                                                                                                                                 <  1:745545/150‑1 (MQ=255)  ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACaa                                                                                                                                                >  2:1032059/1‑150 (MQ=255)  ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACaa                                                                                                                                                <  2:922829/150‑1 (MQ=255)       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataat                                                                                                                                          >  2:436886/1‑151 (MQ=255)       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataat                                                                                                                                          <  2:804613/151‑1 (MQ=255)       gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAaataa                                                                                                                                           <  1:338482/150‑1 (MQ=255)         tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAataata                                                                                                                                         <  1:1078220/150‑1 (MQ=255)               gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATaaaaaa                                                                                                                                  <  2:327540/151‑1 (MQ=255)                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAAt                                                                                                                                 <  2:99570/150‑1 (MQ=255)                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAAt                                                                                                                                 <  1:1051770/150‑1 (MQ=255)                 aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATc                                                                                                                                >  1:455313/1‑151 (MQ=255)                  aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATcc                                                                                                                               <  1:500824/151‑1 (MQ=255)                   aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATccc                                                                                                                              >  1:1161693/1‑151 (MQ=255)                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCa                                                                                                                          <  1:600956/150‑1 (MQ=255)                        gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCAt                                                                                                                         >  2:386029/1‑151 (MQ=255)                         aCCTTTTGGTCCTTTTTTTTCTGCTCGTTTTTATTTCATGAATTCTTCTTGCTGCGCAAGCGTTTTCA‑AAAAAAGGTTAGATAATTTTTTTGTCTCTAAATGCCTGAAAAACATGCG‑TGAGCCACAAAATAATATAAAAAACCCCGCCAt                                                                                                                         <  1:255574/150‑1 (MQ=255)                           cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                       >  2:958210/1‑150 (MQ=255)                           cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                       >  2:285739/1‑150 (MQ=255)                            aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                       >  1:317589/1‑149 (MQ=255)                               tgttCCATTTTTTTCTTCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTCCC‑AAAACAGGTTAGATAATTTTTTTGTCGCTTAATCCCTGTAAAACATGCA‑GGGGCCAAAAAATAATATAAAAAACCCCGCCATTaa                                                                                                                      <  1:751755/144‑1 (MQ=255)                               tGGTCAACTTTTTTATGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                  >  1:926141/1‑151 (MQ=255)                                      cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                            >  2:163559/1‑150 (MQ=255)                                       ttttttCTTCTCTTTTTTATTTCATGTAATCTTCTTGCTGCGCAGGCTTTTCCC‑AAAACATTTAAAATTATCTTTTTTTCGTTTAATGCTTATAAAACATGCA‑TGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGa                                                                                                                 <  2:193037/144‑1 (MQ=255)                                       ttttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                            >  1:691875/1‑149 (MQ=255)                                         ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAg                                                                                                          >  1:296432/1‑149 (MQ=255)                                                 tttttttttttTCATGCATTCTTCTTGCGGCAAAAGCTTTTCCA‑AAAACAGTTAAGATATTTTTTTTGTCTTTTAACGCTTAAAAAACAGGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCa                                                                                                    <  1:639434/144‑1 (MQ=255)                                                   ttttttATTTGATCCCCTCTTCTTCCGGCGCACGCTTTTTCA‑AGAGCTGATAAGATAATTTTTTTGACTGTCAACCCATGTAAAAC‑TGTAGTGAGCCACAAAATACAAGAAAAAACTCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                 <  2:378769/147‑1 (MQ=255)                                                     ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                             >  1:56055/1‑150 (MQ=255)                                                       ttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                             >  1:787566/1‑148 (MQ=255)                                                          tttCATGAATTTTTCTTGCTGCGAAAGCTTTTTCA‑AAAACAGGTTAAATGTTTTTTTTGTCGTTAAATCCTTGAAAAACATGCA‑GGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                        <  1:1024265/150‑1 (MQ=255)                                                                 cAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                >  1:119054/1‑151 (MQ=255)                                                                 cAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGt                                                                                >  2:183782/1‑151 (MQ=255)                                                                       tcttGCGGCGCAAGCTTTTCCC‑AAAACGGGTTAGATGATTTTTTTGTCGTTTAATGCCTAAAAAACATGAA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGc                                                                            <  2:929894/149‑1 (MQ=255)                                                                       tcttGCGGCGCAAGCTTTTCCA‑AAAACAGGTTAGATGTTTTTTTTGTCGTTTAATCCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                              <  2:294597/147‑1 (MQ=255)                                                                          tgctgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                        >  2:956239/1‑150 (MQ=255)                                                                                  aaGCTTTTTCC‑AGAACAGTTTAGATGATTTTTTTGTCGCTTATTGCCTGAAAAAAATGCA‑TGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                 <  1:133604/149‑1 (MQ=255)                                                                                   atgcttttCCAAGAAAAGTTTAAATTATTTTTTTGTCGTTAAATGCCTTAAAACCATGAA‑GGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                 <  1:210903/145‑1 (MQ=255)                                                                                        tttCCAAGAACAGTTTAGATGTTTTTTTTGTCGTTTAATGCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                              <  2:18713/147‑1 (MQ=255)                                                                                        tttCC‑AGACCAGGTAATATAATTTTTTGGCCCCTAATTGCTAGAAAAAAATCCT‑TGAGCCAAAAAAAAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                           <  2:873924/149‑1 (MQ=255)                                                                                            caaaaaCAGGTTAGATATTTTTTTTGTCGTTAATTCCCTGAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                        <  1:937777/145‑1 (MQ=255)                                                                                              aaaagagtttAGATGATTTTTTTTTCGCTAAAGTCCAAAAAACCAGGCA‑TGCCCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAt                                                     <  2:4223/142‑1 (MQ=255)                                                                                              aGAAAAGGTGAGATGTTTTTTTTTCCGTTTAATGCATGAAAAACATGCA‑GGGCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAtt                                                    <  2:657674/151‑1 (MQ=255)                                                                                                aacggtttAGTTGATTTTTTTGTCGCTAAATGCTTGAAAAACATGAA‑TGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                  <  2:967684/145‑1 (MQ=255)                                                                                                      ttAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                             >  1:790851/1‑150 (MQ=255)                                                                                                        aGATGTTTTTTTTGTCGTTAAATGCCTAAAAAACATGCA‑TGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                             <  2:232117/148‑1 (MQ=255)                                                                                                         gatgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAttt                                         >  1:44115/1‑151 (MQ=255)                                                                                                           tgatCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                       >  1:363822/1‑151 (MQ=255)                                                                                                              ttttttttTCGTTTATTGCCTGTAAAACATGCA‑TGACCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                      <  1:331695/147‑1 (MQ=255)                                                                                                              tttttttGTGTCTTAATGCCTAAAAAACAGGCA‑AGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                      <  1:305257/147‑1 (MQ=255)                                                                                                              tttttttGTCTTTTAATGCCTGAAAAACAGGCA‑TGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                      <  2:684083/147‑1 (MQ=255)                                                                                                              tttttttGTCGTTAATTGCCTAAAAAACATGCA‑TGGCCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                       <  1:508635/146‑1 (MQ=255)                                                                                                              tttttttGTCGTTAAATCCCTGAAAAACATGAA‑TGAGCAAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                     <  1:1035455/148‑1 (MQ=255)                                                                                                              tCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                    >  1:608560/1‑151 (MQ=255)                                                                                                               cTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                    >  2:1043668/1‑150 (MQ=255)                                                                                                                ttttttTCGTTTAATGCTTAAAAACCAAGCA‑TTTCCAAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAt                                           <  1:320724/142‑1 (MQ=255)                                                                                                                tttttGTCTTTTAATGCCTAAAAAACATGCA‑TGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                   <  1:1116018/150‑1 (MQ=255)                                                                                                                  tttGTCTTTTAATCCCTAAAAAAAAGGCA‑GGAGCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                 <  1:998252/150‑1 (MQ=255)                                                                                                                   ttGTCTCTTAATCCCTTAAAAACATGCA‑TGACCAAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                <  1:1126109/150‑1 (MQ=255)                                                                                                                   ttGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                               >  2:412587/1‑151 (MQ=255)                                                                                                                   ttGTCGCTTAATCCTTGAAAAACATGCA‑GGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                <  2:909466/150‑1 (MQ=255)                                                                                                                      tCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                            >  1:322850/1‑151 (MQ=255)                                                                                                                          ttAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                        >  1:853697/1‑151 (MQ=255)                                                                                                                          ttAATCCCTGTAAAACATGCA‑TGAGCCAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                            <  2:1105151/147‑1 (MQ=255)                                                                                                                                cctaaaaaaCATGCA‑TGACCCAAAAAATAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                    <  1:436869/144‑1 (MQ=255)                                                                                                                                    aaaaaCACCCA‑TGAGCACAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGATAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                 <  1:368061/147‑1 (MQ=255)                                                                                                                                     aaaaaaTGCA‑TGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa             <  1:147962/151‑1 (MQ=255)                                                                                                                                     aaaaCATGCA‑TGGGCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                 <  1:15190/147‑1 (MQ=255)                                                                                                                                     aaaaCATGCA‑TGAGCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCa               <  2:980624/149‑1 (MQ=255)                                                                                                                                     aaaaCATGCA‑TGAACCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa             <  1:732313/151‑1 (MQ=255)                                                                                                                                         catgca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa          >  2:473533/1‑150 (MQ=255)                                                                                                                                            gca‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg      >  2:838000/1‑151 (MQ=255)                                                                                                                                              a‑tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg      >  2:9944/1‑149 (MQ=255)                                                                                                                                                  aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg  >  2:408880/1‑150 (MQ=255)                                                                                                                                                  aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg  >  1:1108768/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                 TTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCA‑TGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTG  >  NC_000913/29338‑29630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑