Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,984,4410AC53.7% 46.9 / 87.7 54G384G (GGT→GGGyqeFshort chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (24/1);  new base C (0/29);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.78e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.35e-10

CGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAC  >  NC_000913/2984291‑2984589
                                                                                                                                                       |                                                                                                                                                     
cGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCcaca                                                                                                                                                       <  2:721894/151‑1 (MQ=255)
    tatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                                                                                                                                   >  1:582141/1‑151 (MQ=255)
        tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                                                              >  2:755068/1‑151 (MQ=255)
          aTGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATTGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcccc                                                                                                                                            >  1:45684/1‑151 (MQ=255)
                aTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgccg                                                                                                                                       >  2:793213/1‑150 (MQ=255)
                           aGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGtt                                                                                                                           >  2:36291/1‑151 (MQ=255)
                           aGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGt                                                                                                                            >  2:869602/1‑150 (MQ=255)
                               aGCCAAGAAAATTTATGTCGACTTGCCAGCCATCATTTTTCAAAAGATTAATGCCGGCAAATTTTTCTCGTTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTAATTCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGcc                                                                                                                        <  2:37922/150‑1 (MQ=255)
                                    aaaaatTGTTTGTCGACTTGCCACCCATCTTTATTAAACAGATTAATGACGGAAGATATTTATCGGTAAGGAGGAAATGCAGGGCTTTTCGTCACTTTCATTGGTAAATCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCt                                                                                                                  <  2:891110/149‑1 (MQ=255)
                                      aaaaTGTATGTCGCCTGGCCACCAATAATTATCCAACAGATTAATGACGGCAAATATTTATGGGAAAGGAGAAATGGGAGAGTTTTTCTTCATGTTAAAGGGTAAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCtt                                                                                                                 <  1:659572/150‑1 (MQ=255)
                                      aaaaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt                                                                                                                >  1:807895/1‑151 (MQ=255)
                                        aaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATAAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTTTTCGTCACGTTAAATGGTAAATGCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                                                                              <  2:1117423/151‑1 (MQ=255)
                                            tatgtCGACTGGCCCGCCATCTTTATCAAAAAAATAAATGCCGGAAGATTTTTCGCGGTAGGGAGAAAATGCGAGGCTTTTCTTCACTTTCACTGGTAAATGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                                                             <  2:76261/148‑1 (MQ=255)
                                                 cgtcTTGCCACCCATCATTATTCAAAAAATTAAGGGCGGAAGATTTTTATGGGAAGGGAGAAAATGCAGAGTTTTTCTTCCCTTTAATTGGCAAATCCCCCCCCGTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt                                                                                                       <  1:230563/146‑1 (MQ=255)
                                                 cGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc                                                                                                     >  1:662407/1‑151 (MQ=255)
                                                  gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACg                                                                                                    >  1:714906/1‑151 (MQ=255)
                                                  gACTTGCCAGCCATCATTATCCAACAGATAAATGGCGGCAGATTTTTATCGGTAAGGAGAAAATGCAGACTTATACTTCACTTTCATGGGTAAACCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc                                                                                                     <  1:789249/150‑1 (MQ=255)
                                                     ttcccagccaTAATTATTCAACAGATTAATGCGGAAAGATTTTTACCGGAAAGGAGAAAATGCAGAGCTTTTCTTCCCGTTCAATGGTCATTCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAc                                                                                                     <  1:65552/144‑1 (MQ=255)
                                                     ttcccacccaTCTTTATTCAAAAAATAAATACCGGAATATATTTATGGGAAAGGAGAAAACGAAGACTTTTTCGTCAGGTTAAGGGGCCATTGCCCCACCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGt                                                                                                   <  1:1040644/146‑1 (MQ=255)
                                                     tgcccacccaTTATTATCCAACAGATTAATGAGGGCATATTTTTAGGGGTAAGGAGGAAAGGCAGAGCTTTTCGTCGCTTTCAGGGGTAAATGCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGt                                                                                                   <  1:1107692/146‑1 (MQ=255)
                                                       gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc                                                                                                >  1:766737/1‑150 (MQ=255)
                                                        ccacccaTAATTATTCAACAAATAAATGCCGGAAGATATTTATGGGTAAGGAGGAAATGCAGATCTTTTCGTCCCGTTCAATGGTCACCGCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTc                                                                                                <  1:458955/149‑1 (MQ=255)
                                                         cagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc                                                                                             >  1:265047/1‑151 (MQ=255)
                                                                  ttattCAACAGATTAATGCCGGCAGATATTTACCGGTAAGGAGAAAATGCAGACCTTTTCGTCACGTTAAATGGTCAATCCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAt                                                                                           <  1:1161262/144‑1 (MQ=255)
                                                                       cAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAAcc                                                                                 >  2:903900/1‑149 (MQ=255)
                                                                        aaaagtttAATGACGGCAAATATTTATCGGGAGGGAGGAAATGCAGACTTTTTCGTCACGTTCAGTGGCTAATCCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga                                                                              <  2:625078/145‑1 (MQ=255)
                                                                             aTTAAGGACGGCAGTTATTTATGGGAAGGGAGAAAATGCAGACTTATTCGTCTCGATAATTGGTCAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc                                                                         <  1:858157/151‑1 (MQ=255)
                                                                                aaTGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                                                                        >  1:1156304/1‑149 (MQ=255)
                                                                                aaTGACGGCAGATATTTATCGGAAAGGAGAAAATGCAGAGCTTTTCGTCACGCTCAAGGGTAAATGCCACCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                                                                        <  2:215280/149‑1 (MQ=255)
                                                                                aaTGACGGAAGATATTTATGGGTGAGGAGAAAATGAAGAGTTTTTCGTCTTTTTCAAGGTTAAACGCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAcc                                                                        <  2:38330/149‑1 (MQ=255)
                                                                                      ggaaaatatTTATCGGTAAGGAGAAAATGCAGAGCTTTTCGTCACTTTAAATGGTCAATCCCACCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTc                                                                 <  2:824119/145‑1 (MQ=255)
                                                                                      ggCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTAAATGGTAAATCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCg                                                                <  2:534162/151‑1 (MQ=255)
                                                                                         agttatTTAGCGGTAGGGAGGAAATGCAGGCCTTTTCGTCTCGTCAAAGGGTAAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCgg                                                               <  1:692476/146‑1 (MQ=255)
                                                                                              tttATCGGAAAGGAGAAAATGCAGATTTTTTCGTCACTTTAATTGGTCAACGCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAc                                                           <  1:1153662/148‑1 (MQ=255)
                                                                                                    ggaagggagaaAATGCAGATCTTTTCGTCCCTTTCAAGGGTCAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgc                                                    <  1:137746/144‑1 (MQ=255)
                                                                                                    ggaaaGGAGAAAATGAAGAGCTATTCGTCACGTTAAATGGTCAATCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgc                                                    <  2:738952/146‑1 (MQ=255)
                                                                                                    ggTAAGGAGAAAATGCAGAGCTTTTCGTCACGTTAAATGGTAAATCCCCCCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAgc                                                    <  2:652789/149‑1 (MQ=255)
                                                                                                      tAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCt                                                >  2:1141580/1‑151 (MQ=255)
                                                                                                       aaGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCt                                                >  2:1084429/1‑150 (MQ=255)
                                                                                                       aaGGAGAAATTGCAGAGTTTTTCGTCACTTTAATGGGTAAAGCCCCCCCCCGGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCt                                                <  2:347236/150‑1 (MQ=255)
                                                                                                         ggaggaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATc                                             >  1:416298/1‑151 (MQ=255)
                                                                                                             gaAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCggg                                          >  2:136723/1‑150 (MQ=255)
                                                                                                              aaaTGCAGAGCTTTTCGTCACGTTAAAGGGTAAATCCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCggg                                          <  2:681290/149‑1 (MQ=255)
                                                                                                              aaaGGCAGAGTTATTCGTCCCTTTAATGGGTAATCCCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTg                                        <  2:244221/151‑1 (MQ=255)
                                                                                                               aaTGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTg                                        >  2:575840/1‑150 (MQ=255)
                                                                                                               aaTGAAGAGCTTTTCGTCACGTTAAATGGTCAATCCCACCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGa                                       <  2:995287/151‑1 (MQ=255)
                                                                                                                aTGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCTCCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAc                                      >  2:78480/1‑151 (MQ=255)
                                                                                                                     gagtttttCGTCCCTTTCAAGGGTCAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGAcc                                     <  2:1097219/141‑1 (MQ=255)
                                                                                                                     gagCTTTTCGTCACGTTCAAGGGTCAATGCCCCCCCCGGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGt                                 <  1:182664/151‑1 (MQ=255)
                                                                                                                                  cGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccac                    >  1:117586/1‑151 (MQ=255)
                                                                                                                                   gTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGACAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGccacc                   >  1:77405/1‑151 (MQ=255)
                                                                                                                                       aagggTCAATGCCACCCCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg               <  1:17665/148‑1 (MQ=255)
                                                                                                                                        aTGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattg               >  2:260986/1‑150 (MQ=255)
                                                                                                                                             cAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACcattgacattg         >  2:162588/1‑151 (MQ=255)
                                                                                                                                                   ccACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCg    >  2:648641/1‑151 (MQ=255)
                                                                                                                                                     acacCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc  >  1:995164/1‑151 (MQ=255)
                                                                                                                                                     acacCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAc  >  1:890873/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGAC  >  NC_000913/2984291‑2984589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: