Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,350,844	0	A	C	45.1%	76.8 / 12.7	51	L728V (TTA→GTA)	arcB	aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base A (24/4);  new base C (0/23);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.27e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTC  >  NC_000913/3350699‑3350993                                                                                                                                                  |                                                                                                                                                      gTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCa                                                                                                                                                  <  2:197035/151‑1 (MQ=255)   ggCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACt                                                                                                                                                <  2:276084/151‑1 (MQ=255)    gCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTg                                                                                                                                               <  2:820692/151‑1 (MQ=255)        ttGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGAcc                                                                                                                                            <  1:7523/150‑1 (MQ=255)                  cGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCt                                                                                                                                 >  2:943383/1‑151 (MQ=255)                   gCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGTAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCtt                                                                                                                                >  1:871380/1‑151 (MQ=255)                         aGCTCTTCTCCGTCGTGACCCCCCTCTTTTTTCATCTCTCCAACCCTTCCCCCGACGTTTTCTCCCCAGGCCGGAAGGCCGGGAGACTAAATTTGCTGCCCCAGTTGTTGAAGATGGCGTACCCCCCCTCACCCCGCCGCACCTTTAAtt                                                                                                                           <  1:63202/150‑1 (MQ=255)                           cACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAAtttt                                                                                                                         >  2:541783/1‑150 (MQ=255)                              ttCTACGTCGTGCCCCCCCTCTTCTTTCATCTCTTCAACCCATCCCCCGACGTTATCTCCCCAGGCGGAAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTAt                                                                                                                       <  2:568602/149‑1 (MQ=255)                                   cgtcgtGCCCCCACTTTTTTTTCATCTCTTCAACCCATCCCCCGACGTTATCTCCCCAGGCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCGGCCCCCGCCGCACCTTTAATTTTATGtcct                                                                                                                  <  2:266240/149‑1 (MQ=255)                                    gtcgtGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGtccttcc                                                                                                               >  2:1100450/1‑151 (MQ=255)                                             cctcttttcttTCATCTCTTCAACCCATCCCCCGCCTTTATCTCCCCGGGCCGGAAGGTCAGGAGACTGATTTTGCTGCCCCAGTTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTcaa                                                                                                           <  2:753677/140‑1 (MQ=255)                                             ccccttttcttTCATCTCTTCAACCCATCCCCCGACTTTATCTCCCCGGGCCGGAAGGTCAGGAGACTGAATTTGCTGCCCCAGTTTTTGCAGATGGCGAACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAAt                                                                                                       <  1:1160113/144‑1 (MQ=255)                                             cccctctTTTTTCATCTCTACAACCCATCCCCCGACTTTATCTTCCCAGGCGGAAAGGTGAGGAGACTAATTTTGCTGCCCCGGCTGTTGCAGAGGGCGTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATg                                                                                                      <  2:733231/148‑1 (MQ=255)                                                   tttttccATCTCTTCAATCCATCCCCCGCCGTTATCTCCCCAGGCGGGAAGGTCGGGAGACTGATTTTGCTGCCCCAGCTTTTGAAGATGGCTTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCt                                                                                                   <  2:562166/142‑1 (MQ=255)                                                    tcttTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCttttt                                                                                               >  2:240321/1‑151 (MQ=255)                                                      tttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                              >  1:391321/1‑150 (MQ=255)                                                      tttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                              >  2:1070335/1‑150 (MQ=255)                                                       ttCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTAt                                                                                            >  1:281579/1‑151 (MQ=255)                                                       ttCATCTCCACAATCCATCCCCCGTCTTTTCCTCCCCAGGCCGAAAGTTCGGAAAACGAATTTTGCTCACCCAGTTGTTGGAGATGGATTACCCCAACGCCCTCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCtttttt                                                                                              <  1:330917/149‑1 (MQ=255)                                                        tCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATc                                                                                           >  1:766833/1‑151 (MQ=255)                                                        tCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATc                                                                                           >  2:182523/1‑151 (MQ=255)                                                         cATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATcc                                                                                          >  1:1079030/1‑151 (MQ=255)                                                          aTCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCt                                                                                         >  2:486885/1‑151 (MQ=255)                                                          aTCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCGTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCt                                                                                         >  2:190086/1‑151 (MQ=255)                                                            ctctTCAACCCATCCCCCGACGTTATCTCCCCGGGCGGAAAGGTAGGGAGACTAATTTTGCTGCCCCAGCTTTTGCAGAGGGCTTACCCCCACGGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATc                                                                                           <  2:713055/147‑1 (MQ=255)                                                              ctTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTgcgc                                                                                     >  1:321042/1‑151 (MQ=255)                                                                 cAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGt                                                                                  >  1:213178/1‑151 (MQ=255)                                                                  aatccttcaacggatgTTATCTTCCCAGCCGGGAGGGTGAGAAGACTAAATTTGCAGCCCCAGTTTTTGCAGAGGGCGTACCCCCACTACCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTc                                                                                 <  1:273931/136‑1 (MQ=255)                                                                       aTTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGAtt                                                                            >  1:1164737/1‑151 (MQ=255)                                                                          ccccccctttATCTCCCCAGGCGGGAAGGTCAGGAGACTAATTTTGCTGCCCCATTTTTTGAAGATGGCTAACCCCCACTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGcc                                                                                    <  1:922469/132‑1 (MQ=255)                                                                          cACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTca                                                                          >  2:1060388/1‑149 (MQ=255)                                                                                 gTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGc                                                                  >  2:538091/1‑151 (MQ=255)                                                                                  tcaTCTCCCCAGGCGGGAAGGGCAGGAGACTGATTTTGCTCACCCAGTTGTTGCAGATGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGc                                                                  <  1:519263/148‑1 (MQ=255)                                                                                       ttCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTg                                                            >  1:1069095/1‑151 (MQ=255)                                                                                        ccccAGGCGGGAGGGTCAGGAGACTAATTTTGCTGCCCCAGTTTTTGCAGATGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGa                                                           <  1:391845/150‑1 (MQ=255)                                                                                          ccggccgggAAGGTAAGGAGACTAATTTTGCTGCCCCGGCTGTTGCAGAGGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACa                                                         <  2:236108/144‑1 (MQ=255)                                                                                             ggCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACAt                                                        >  1:419789/1‑149 (MQ=255)                                                                                              gCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGc                                                     >  1:1101976/1‑151 (MQ=255)                                                                                                 ggAAGGTCAGGAGACTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCg                                                  <  2:184360/151‑1 (MQ=255)                                                                                                   aaGGTCAGGAGACTAAATTTCCGGCCCCAGCTGTTGCAGAGGGCTTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCgg                                                 <  2:973291/150‑1 (MQ=255)                                                                                                     ggtaaggactcaaattttGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCGACCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcat                                              <  2:640477/136‑1 (MQ=255)                                                                                                     ggTCGGGAGATTGATTTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGcat                                              <  2:129097/151‑1 (MQ=255)                                                                                                        cAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGACGTCAGATTCGACTCAAGCACGCTGACATAGAGCGGcatca                                            >  2:978744/1‑150 (MQ=255)                                                                                                         aGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCatcatc                                          >  1:1050819/1‑151 (MQ=255)                                                                                                             gaCTGAATTTGCTGCCCCAGCTGTTGCAGATGGCGTACCCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCAtcttct                                      <  2:493387/151‑1 (MQ=255)                                                                                                                  attttCCTGCCCCGCCTGTTGCATTTGGCGTACCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCaaa                                  <  2:712878/148‑1 (MQ=255)                                                                                                                    tttGCTGCCCCAGCTTTTGCAGATGGCGTACCCCCCCTCCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTtctc                                     <  1:544705/145‑1 (MQ=255)                                                                                                                         tGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                           >  1:592133/1‑150 (MQ=255)                                                                                                                            cccAGCTGTTGCAGATGGCGTACCCCCCCTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCt                           <  2:286789/147‑1 (MQ=255)                                                                                                                                              cGTACCCCCCCTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTc      <  1:48442/150‑1 (MQ=255)                                                                                                                                                 aaCCCCACTGCCCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTc  <  2:777099/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                      GTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCAGGAGACTGAATTTGCTGACCCAGCTGTTGCAGATGGCGTAACCCCACTGACCCCGCCGCACCTTTAATTTTATGTCCTTCCTCAACAATGCCTTTTTTATCCTGCGCCGTCAGATTCGACTCCAGCACGCTGACATAGCCCGGCATCATCTTCTCAAACACCGCTAACCCGTCGGTGATCAGCTTCGGTC  >  NC_000913/3350699‑3350993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑