Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	45.3%	122.2 / 22.6	75	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (39/2);  new base C (0/34);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 2.51e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTA  >  NC_000913/3443133‑3443421                                                                                                                                                   |                                                                                                                                                 gCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                            <  1:570102/151‑1 (MQ=255)  cATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                           >  1:374549/1‑151 (MQ=255)           acacATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCAc                                                                                                                                  >  2:1085664/1‑151 (MQ=255)             acaTAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGGTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                                >  1:546867/1‑151 (MQ=255)               aTAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                              >  1:1051216/1‑151 (MQ=255)                aaaaGCGGTGCCCCA‑GGCTAAAAAAACAGAAAATTTTTTGTCACCCATTCCCAACCAGTCCCCCCCCCAAACAATGACGCGATGGTCGCCGGGAAC‑AAAATAATCCTGAAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCa                                                                                                                             <  1:88086/150‑1 (MQ=255)                    gCGGTTCCCCC‑GCCTGAAAATCCAGCAAAATTGTTGTCAGCCGGTCCCACCCGATACCCCCCCGGAACAAGAACGGGATGGCCGCCGGAAAC‑ATAAAAATACTGGAAGGGAGGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCg                                                                                                                          <  1:446568/150‑1 (MQ=255)                         tgccccggtcTGAAAATAGAGAAAATTTGTTGCCAGCCGGTCCAAACCAGTCCCCCCCCCAACCCAGGCCGCGATGGTCGCGGGGAAA‑AAAATAATACTGGAAGCGAAGATTGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                       <  1:846966/140‑1 (MQ=255)                         tGCCCAGGGCTAAAAATACAGCAAATTTGTTGTCACCCATTCCCACCCAGCCCCCCCCCCAACCCAGGCCGGGAGGGCCGCGGGAAAA‑AAAATAATACGGAAAGCAAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTaa                                                                                                                      <  2:926073/150‑1 (MQ=255)                            ccA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAAtgtg                                                                                                                 >  2:609297/1‑151 (MQ=255)                                ggctaaaaaTACAGCGAATTTGTTGTCAGCCATTTCCACCCAGTCCCGCCCCCGAACCATGACGGGATGGTCGCGGGAAAA‑AAAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtgtgt                                                                                                                <  2:177722/143‑1 (MQ=255)                                  cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                            >  2:520501/1‑151 (MQ=255)                                  cTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                            >  1:874471/1‑151 (MQ=255)                                    gCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                          >  2:694789/1‑151 (MQ=255)                                      aaaaACAGGAAAATTGTTGCCAGCCAGTCCAACCCAGCCCCCCCCCCAAACCATGCGGCGATGGCCGCCGGAAAA‑AAAATAATACGGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                          <  1:412230/149‑1 (MQ=255)                                      aaaTACAGCGAATTTGTTGTCAGCCATTCCCAACCAGTCCCCCCCCCAAACCAGGAGGGGATGGTCGCGGGAAAA‑AAAATAATACGGGAGGCGAAAATTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                          <  1:803344/149‑1 (MQ=255)                                         tACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                       >  1:101370/1‑149 (MQ=255)                                          aCAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                    >  2:1094211/1‑151 (MQ=255)                                               gAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                  >  1:1131863/1‑148 (MQ=255)                                                aattttttgtCGGCCATTCCCACCCAGTCCCCCCCCAGAACCATGCCGGGATGTCCGCGGCGAAC‑AAAATAAAACTGGAGGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                  <  2:1025633/141‑1 (MQ=255)                                                aatttgttgCCAGCCATTCCCACCCAGTCCCGCCCCCGACCCAGGACGCGAGGGTCGCCGGAAAC‑AAAATAATACGGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgcagc                                                                                                    <  2:795743/142‑1 (MQ=255)                                                 aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                              >  2:958267/1‑150 (MQ=255)                                                  aTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                            >  2:1062248/1‑151 (MQ=255)                                                   tttttgtCAGCCAGTCCCACCCATCCCCGCCCCCGAACCAGGCCGGGATGGTCGCGGGGAAC‑AAAATAATCCTGGAAGCGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                            <  2:935266/147‑1 (MQ=255)                                                    ttttgtCGCCCATTCCCAACCAGCCCCGCCCCCGACCCATGACGGGATGGTCGCCGGAAAC‑AAAAAAATACTGAAAGAGAAGATTCCCGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                            <  2:6554/147‑1 (MQ=255)                                                     gttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                         >  1:382547/1‑151 (MQ=255)                                                      ttgtCAGCCAGTCCAACCCAGTCCCCCCCCCGACCCAGGCCGGGGGGGTCGCCGGAAAA‑AAAATAATACGGGAAGCAAAGATTGGGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                          <  1:48778/149‑1 (MQ=255)                                                        gtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                      >  2:919760/1‑151 (MQ=255)                                                         tCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                     >  2:151266/1‑151 (MQ=255)                                                          cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                    >  1:899235/1‑151 (MQ=255)                                                                 ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                             >  2:403230/1‑151 (MQ=255)                                                                 ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                             >  1:63419/1‑151 (MQ=255)                                                                 ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                             >  2:1044275/1‑151 (MQ=255)                                                                   ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                           >  1:731375/1‑151 (MQ=255)                                                                   ccAACCAGCCCCCCCCCCAAACCATGGCGCGATGGTCGCCGGGAAA‑AAAATAATACGGAAAGCGAAGATTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                             <  1:1087834/149‑1 (MQ=255)                                                                    cAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                          >  1:329139/1‑151 (MQ=255)                                                                     aaCCAGTCCCGCCCCCGAACCAGGACGGGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                         <  2:266876/151‑1 (MQ=255)                                                                     aaCCAGCCCCCCCCCAGAACCATGCCGCGATGGTCGCGGGAAAA‑AAAAAAATACGGGAAGAGAAATTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                           <  2:952481/149‑1 (MQ=255)                                                                       cgaGTCCCCCCCACGAACCATGACGCGATGGTCG‑CGGGAACAAGAATAATACTGAAAGAAAAGAGTGGGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcg                                                                       <  2:1094465/149‑1 (MQ=255)                                                                       ccAGTCCCCCCCCCAAACCATGACGCGATGGTCGCGGGGAAA‑AAAATAATACTGAAAGCGAAGTTTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                          <  2:97581/148‑1 (MQ=255)                                                                        cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                      >  2:805487/1‑151 (MQ=255)                                                                        cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGAGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTcgc                                                                      >  2:161444/1‑151 (MQ=255)                                                                           tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                    >  2:596784/1‑150 (MQ=255)                                                                             ccGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AAAATAATACTGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                    <  2:490250/148‑1 (MQ=255)                                                                              cccccccAAACAATGACGCGAGGGTCGCCGGAAAA‑AAAAAAATACTGGAAGGGAAGATGGCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                <  1:142405/149‑1 (MQ=255)                                                                              cGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                >  1:710817/1‑151 (MQ=255)                                                                              cGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGtt                                                                >  2:748884/1‑151 (MQ=255)                                                                                 ccccGAACCAGGCCGGGAGGGTCGCGGGAAAA‑AAAAAAATACTGAAAGCAAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                             <  2:905759/151‑1 (MQ=255)                                                                                     gAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcac                                                          >  1:785587/1‑150 (MQ=255)                                                                                      acccaggcggcgATGGTCGCCGGAAAA‑AAAAAACTCCGGGAAGGGAAGAGTGGCGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACaa                                                        <  1:374113/142‑1 (MQ=255)                                                                                      aaCCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                         >  2:388723/1‑150 (MQ=255)                                                                                               gcgGTGGCCGCGGGAAAA‑AAAAAAATACGGGAAGGAAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                               <  1:926293/151‑1 (MQ=255)                                                                                               gcgATGGTCGCGGGAAAA‑AAAATAATTGGGGAAGCGAAGTTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                               <  1:1092237/151‑1 (MQ=255)                                                                                               gcgATGGTCGCCGGAAAC‑AAAAAAATACGGGAAGCAAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                  <  2:457118/148‑1 (MQ=255)                                                                                                  aTGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                            >  1:1047246/1‑151 (MQ=255)                                                                                                    ggTCGCCGGAAAA‑AGAATAATGCGGAAAGCGAAGTTGGCCGGATTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                            <  1:308932/149‑1 (MQ=255)                                                                                                         ccGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                     >  2:715437/1‑151 (MQ=255)                                                                                                          cGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                     >  1:106247/1‑150 (MQ=255)                                                                                                           gggAAA‑AAAATAATGTTGGAGGGGAAGTTCGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                    <  2:1053050/150‑1 (MQ=255)                                                                                                               aC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaacaa                                >  1:599080/1‑150 (MQ=255)                                                                                                               aC‑AAAATAATACTGGAAGCAAAGTTTGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACaaa                               <  2:557979/151‑1 (MQ=255)                                                                                                                  aaaataatACTGGAAGCGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATa                             <  2:1016195/149‑1 (MQ=255)                                                                                                                   gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                            >  1:545518/1‑151 (MQ=255)                                                                                                                          aCTGAAAGCGAAGTTTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACaaa                      <  2:727465/150‑1 (MQ=255)                                                                                                                             ggAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                  <  1:902582/151‑1 (MQ=255)                                                                                                                               aaGCGAATATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                <  1:195439/151‑1 (MQ=255)                                                                                                                                   gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt            >  2:264028/1‑151 (MQ=255)                                                                                                                                    aagcttccggggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTg           <  2:942474/142‑1 (MQ=255)                                                                                                                                     aaaTTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc          <  1:694766/149‑1 (MQ=255)                                                                                                                                     aGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACACATACAACAAAGAACGTCACTGc          >  2:540600/1‑151 (MQ=255)                                                                                                                                      gATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGc          >  1:1032831/1‑150 (MQ=255)                                                                                                                                         tGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat      >  2:1024528/1‑151 (MQ=255)                                                                                                                                            cGGGATTACCCCCGCCATATTCACTTTCAGCGTTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact   >  1:125904/1‑151 (MQ=255)                                                                                                                                             ggtaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac    <  1:6336/146‑1 (MQ=255)                                                                                                                                             gggATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta  <  1:80736/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCATAGAGTAACACATAAAGCGGTTGCCCA‑GGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAAC‑AGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTA  >  NC_000913/3443133‑3443421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑