Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA43.2% 127.1 / 53.5 95intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (47/7);  new base A (0/41);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 6.31e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAA  >  NC_000913/1824907‑1825203
                                                                                                                                                       |                                                                                                                                                    
ccGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTgagaagag                                                                                                                                                      <  2:1102408/150‑1 (MQ=255)
       tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGagaaaagaa                                                                                                                                               <  2:1578550/150‑1 (MQ=255)
        ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                              <  2:1190854/150‑1 (MQ=255)
        ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                              >  3:177932/1‑150 (MQ=255)
         tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAgaaaagaaa‑a                                                                                                                                            <  3:350388/150‑1 (MQ=255)
          aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑Acc                                                                                                                                          <  2:1035341/151‑1 (MQ=255)
          aCGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑Acc                                                                                                                                          <  2:397223/151‑1 (MQ=255)
           cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑Acc                                                                                                                                          <  2:1064904/150‑1 (MQ=255)
              atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑Accgccg                                                                                                                                      >  2:1044288/1‑151 (MQ=255)
               tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑Accgccg                                                                                                                                      >  2:281534/1‑150 (MQ=255)
                atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGAt                                                                                                                                    >  3:244114/1‑151 (MQ=255)
                 tCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATc                                                                                                                                   >  2:642311/1‑151 (MQ=255)
                   aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCt                                                                                                                                 >  3:899772/1‑151 (MQ=255)
                   aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCt                                                                                                                                 >  3:823457/1‑151 (MQ=255)
                     aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGt                                                                                                                               >  3:1469239/1‑151 (MQ=255)
                     aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGt                                                                                                                               >  3:628588/1‑151 (MQ=255)
                       ttttCCATCCCCGCCCCCGGCCCTTTTTTTTTTTTTTTATCGTTGGGGTACCGGTGGATGGTTAT‑CGGCAACGGGGGTGATTTACGTTAGTGGTGATCGGTTTTTTTTA‑ACATCTGTTTGAGAAGAAAAAAAAAA‑CCCGCCGATCCTGt                                                                                                                               <  3:877631/147‑1 (MQ=255)
                         ttCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCAc                                                                                                                           >  2:1127699/1‑151 (MQ=255)
                         ttCCATACTCGCTCCCGGCCAGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCAc                                                                                                                           >  3:1149404/1‑151 (MQ=255)
                           ccATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCg                                                                                                                         >  2:953429/1‑151 (MQ=255)
                            cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGc                                                                                                                        >  3:1470437/1‑151 (MQ=255)
                               ccccccccccGCCCCTTTTTTTTCATTTTTTTCTTTCAGGAACCCGTGGATGTTTCT‑CGCCAGCCGGGTTAATTTACGTTAGGGGTGATCAGTTTTTTTTT‑CCACTTTTTTGAGAAGAAAAAAGAAA‑ACCGCCGATCCTGTCCACCGCAt                                                                                                                      <  3:12547/143‑1 (MQ=255)
                                cTCGCCCCCGGCCTTTTTTTTTCATTTTTATCGTTCAGGTAACGGTGGATGGTTAT‑CGGAACCTGGGGTTTTTTACGTTAGTGGTGATCAGTTTTTTTTT‑AAAACTTTTGGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCa                                                                                                                       <  2:396508/148‑1 (MQ=255)
                                      ccgggCCCGTTCTTTTCTTTTTTATCGTTCAGGTAACCGTGGATGTTTAT‑CGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTT‑ACAACTGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCaa                                                                                                              <  2:158832/148‑1 (MQ=255)
                                           cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                           >  2:544494/1‑149 (MQ=255)
                                            ccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                        >  2:1435710/1‑151 (MQ=255)
                                               tttttttCATTGTTATCGTTAAGTTACCGGTGGTTGTTTAT‑CGGCACCTGGGGTGTTTTACGTTGGTGGTGATAGGGTTTTTTTA‑ACAATTTTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGt                                                                                                        <  2:1270268/145‑1 (MQ=255)
                                                ttttttCATTGTTATCGTTCAGGTACCGGGGGATGGTTAT‑CGGCAACTGGGGTGTTTTACGTTAGTGGTGATAAGGTTTTTTTT‑ACACCTGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa                                                                                                     <  2:1580259/148‑1 (MQ=255)
                                                  ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                   >  2:414780/1‑150 (MQ=255)
                                                   tttCTTTTTTATCGTTCGGTTACCCGTGGTTGTTTTT‑CGGCACCTCGGGTAATTTACGTTAGTGGTGACCGGGTTTTTTTT‑ACATGTTTTGAAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa                                                                                                  <  2:870073/150‑1 (MQ=255)
                                                   tttCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                   >  3:1079241/1‑149 (MQ=255)
                                                        tttttATCGTGAGGGTACCCGGGGTTGTTTAT‑CGGAAACTGGGTTGTTTTACGTTAGTGGTAATCGGTTTTTTTTT‑ACACCTGTTTGAGAAGAAAAAAGAAA‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                             <  3:37910/147‑1 (MQ=255)
                                                        ttGTTTTCTTTCAGGTACCCGGGGTGGTTTAT‑CGGAAACTGGGGTGTTTTACGTTGGGGGTAATCAGTTTTTTTTT‑ACAATTGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                             <  2:1479929/150‑1 (MQ=255)
                                                        ttGTTTTCGTTCAGGTACCGGTGGATGTTTAT‑CGGCAACTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTT‑ACATTTTTTGGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa                                                                                                <  2:845993/147‑1 (MQ=255)
                                                        ttGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                            >  3:1341274/1‑151 (MQ=255)
                                                         tGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                           >  2:1639502/1‑151 (MQ=255)
                                                          tttATCGTTCAGGTACCCGGGGAGGTTTAT‑CGGCACCTCGGGTGATTTCCGTTAGTGGTGATCGGTTTTTTTTT‑ACAACTGTTTGAAAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                           <  3:1271681/149‑1 (MQ=255)
                                                             aTCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                        >  2:1411469/1‑150 (MQ=255)
                                                                gTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                     >  3:1031906/1‑150 (MQ=255)
                                                                gTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                      >  3:897380/1‑149 (MQ=255)
                                                                 ttcggagacccGTGGATGGTTAC‑CGGAATCTC‑GGAGAATTATGTTAAGGGGTAACCGGCTTTTTTTAAAAACTGTTTGAGAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                     <  3:695204/140‑1 (MQ=255)
                                                                 ttaaGGTACCTGTGTAT‑GTTATCCGGCAACCGGGGTTATTGTCTTTAGTGGTAATGTGTTTTTTTTA‑ACAACTTTTTCAGAA‑AAAAAAGAAACACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                    <  2:614639/147‑1 (MQ=255)
                                                                 ttCAGGTACCCGTGGATGTTTAT‑CGGCAACTGGGGTGTTTTCCTTTAGTGGTGATCAGGTTTTTTTT‑ACAACTTTTGGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                        <  2:39878/146‑1 (MQ=255)
                                                                  taggttaccgggggAGGGTTAT‑CGCAACCTGGGGTGTTTTACTTTAGTGGTAACCGGGTTTTTTTA‑ACACCTTTTGGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa                                                                                   <  3:767501/138‑1 (MQ=255)
                                                                  taaGGTACCGGGGGGTGGTTAC‑CGGCACCTGGGGTGATTTACTTTAGTGGTGTTCGGTCTTTTTTT‑ACAACTGTTGGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt                                                                                      <  2:1215008/145‑1 (MQ=255)
                                                                     ggtaccgggggATGTTTAT‑CGGCAACTGGGGTGATTTATGTTAGTGGTGACCGGTTTTTTTTT‑ACAACTTTTTGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAg                                                                                  <  3:298721/139‑1 (MQ=255)
                                                                          ccgggtaTGGTTAT‑CGGCAACTGGGGTGTTTTACGTTGGGGGTAATCGGGTTTTTTTT‑ACACTTTTTTAAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                             <  2:996430/142‑1 (MQ=255)
                                                                            ggggATGGTTAG‑CGCAAACTGGGGTGTTTTACTTTGGTGGGGATCGGTCTTTTTTT‑ACACCTTTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                          <  2:1469378/147‑1 (MQ=255)
                                                                            gTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                            >  2:1546772/1‑147 (MQ=255)
                                                                                aTGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                     >  2:779718/1‑150 (MQ=255)
                                                                                    ttAC‑CGGCATCTCGGTTGATTTACTTTAGTGGTAATCGGGTTTTTTTT‑ACATCTGTTTGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAg                                                                   <  3:1242982/148‑1 (MQ=255)
                                                                                     tAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                >  3:1231650/1‑150 (MQ=255)
                                                                                     tAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                >  2:37870/1‑150 (MQ=255)
                                                                                          ggCACCTGGGGTGTTTTACTTTAGTGGTGATCAGTTTTTTTTT‑ACAATTTTTTGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt                                                             <  2:932372/149‑1 (MQ=255)
                                                                                            cAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                         >  3:15499/1‑151 (MQ=255)
                                                                                             accgggggTGATTTAGGTTAGTGGTGATCAGGTTTTTTTT‑ACAACTTTTGGAGAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                        <  3:1372011/146‑1 (MQ=255)
                                                                                             aaCTGGGGTGATTTACGTTGGTGGTGATCGGTTTTTTTTT‑ACAATTTTTGGAGAAGAAAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                          <  3:1500451/149‑1 (MQ=255)
                                                                                             aaCTCGGTTAATTTACGTTAGTGTTGATCAGGTTTTTTTT‑ACAACTGTTGGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGTCAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag                                                          <  2:741212/149‑1 (MQ=255)
                                                                                                 ggggTGATTTAGGTTAGGGGTAACCGGTTTTTTTTA‑ACAACTTTTAAAGAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                    <  3:316598/150‑1 (MQ=255)
                                                                                                  ggttaaTTTCCTTTAGTGGTGATCAGGTTTTTTTT‑AAAACTTTTGGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                      <  3:1058309/143‑1 (MQ=255)
                                                                                                  gggTGTTTTACGTTAGGGGTGATCAGGTTTTTTTT‑ACAACTGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                   <  2:1343290/151‑1 (MQ=255)
                                                                                                    gTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGc                                                   >  3:535636/1‑149 (MQ=255)
                                                                                                     tGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                >  2:1438225/1‑151 (MQ=255)
                                                                                                     tGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTGAAAGATTATTCAGCAGTTGCAgg                                                >  3:177691/1‑151 (MQ=255)
                                                                                                       aTTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGc                                               >  3:1345476/1‑150 (MQ=255)
                                                                                                        tttACGTTAGTGTGGATGGGTTTTTTTTT‑ACACGGGTTGAAAAAGAGAAAATAAG‑TCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCGTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                              <  3:201996/150‑1 (MQ=255)
                                                                                                             tttggtggtgATCGGTTTTTTTTT‑ACAACTTTTTGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                          <  2:327438/145‑1 (MQ=255)
                                                                                                              ttgggggtgATCAGGTTTTTTTT‑CCAACTGTTTGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                         <  2:754537/144‑1 (MQ=255)
                                                                                                              ttAGTGGTGATCGGGTTTTTTTT‑ACACCTTTTTGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                        <  2:1611020/150‑1 (MQ=255)
                                                                                                                 gtggtgATCGGTTTTTTTTT‑ACAATTTTTTGAGAAGAGAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                     <  3:1358127/150‑1 (MQ=255)
                                                                                                                    gtgATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTTAGTTTTTTGGCTGAAAGATTATTCAGGAGTTGCAGGCATTTTACCTTTTg                                  >  3:1445709/1‑150 (MQ=255)
                                                                                                                     tgATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                >  3:1337047/1‑151 (MQ=255)
                                                                                                                     tgATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                >  3:551912/1‑151 (MQ=255)
                                                                                                                       accagttttttttt‑ACAACTTTTGGAAAAGAAAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                   <  2:1473204/139‑1 (MQ=255)
                                                                                                                       aTCAGGTTTTTTTT‑ACACCTGTTAGAGAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                <  2:193716/149‑1 (MQ=255)
                                                                                                                       aTCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGc                              >  2:1210843/1‑151 (MQ=255)
                                                                                                                          aGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                           >  2:829447/1‑151 (MQ=255)
                                                                                                                            ttttttttt‑ACAACTGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                            <  3:882581/146‑1 (MQ=255)
                                                                                                                            gCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTTAACAGTTGCAGGGATTTTACCTTTTGCCGCCGGAc                         >  3:590353/1‑151 (MQ=255)
                                                                                                                              ttttttt‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACg                        >  3:1217263/1‑150 (MQ=255)
                                                                                                                              ttttttt‑AAAATTTTTTGAAAAGAAAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAc                         <  2:317333/149‑1 (MQ=255)
                                                                                                                               tttttt‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTGGGCGtt                      >  3:882602/1‑151 (MQ=255)
                                                                                                                               tttttt‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                      >  3:811246/1‑151 (MQ=255)
                                                                                                                               ttttta‑ACATCTGTTGGAGAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGt                       <  2:784568/150‑1 (MQ=255)
                                                                                                                                ttttt‑ACACTTTTTTGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                      <  3:1476018/150‑1 (MQ=255)
                                                                                                                                ttttt‑ACACTTTTTGGAAAAAAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                      <  3:862852/150‑1 (MQ=255)
                                                                                                                                  ttt‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                    >  2:1293619/1‑150 (MQ=255)
                                                                                                                                   ttaacacCTTTTAGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                    <  3:16072/147‑1 (MQ=255)
                                                                                                                                    t‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                    >  3:1076204/1‑148 (MQ=255)
                                                                                                                                          cTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg            >  2:361423/1‑151 (MQ=255)
                                                                                                                                           tGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg            >  2:77642/1‑150 (MQ=255)
                                                                                                                                           tGTTTGAAAAGAAAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                <  3:628076/146‑1 (MQ=255)
                                                                                                                                            gTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg            >  2:808108/1‑149 (MQ=255)
                                                                                                                                            gTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc           >  2:721715/1‑150 (MQ=255)
                                                                                                                                              ttGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTTAGACGCtt         >  3:1614749/1‑150 (MQ=255)
                                                                                                                                              ttGAGAAGAGAAAAAAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt         <  3:1372638/150‑1 (MQ=255)
                                                                                                                                              tggagaaaagaaAAAAAA‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt         <  3:537517/148‑1 (MQ=255)
                                                                                                                                              tggaaaaaagaaaaaaaa‑CCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt         <  3:318511/142‑1 (MQ=255)
                                                                                                                                                    agagaaAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa   >  2:171615/1‑150 (MQ=255)
                                                                                                                                                     gagaaAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaaa  >  3:1207591/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
CCGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTAT‑CGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTT‑ACAACTGTTTGAGAAGAGAAAAGAAA‑ACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAA  >  NC_000913/1824907‑1825203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑