Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,589	0	A	T	41.4%	89.8 / 40.0	70	L135F (TTA→TTT)	yegJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (6/35);  new base T (27/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.94e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAC  >  NC_000913/2151442‑2151738                                                                                                                                                     |                                                                                                                                                          cATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTg                                                                                                                                                        <  2:983080/151‑1 (MQ=255)  aTGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATATCAATAATACCTTAtt                                                                                                                                                         >  3:653799/1‑149 (MQ=255)   tGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATAACATTTTTCCTTTTTTTGt                                                                                                                                                      >  2:825757/1‑151 (MQ=255)            gCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑Tcc                                                                                                                                           <  2:919453/151‑1 (MQ=255)             cGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTACCATTGATTCTTTTTTTGTTAcc                                                                                                                                                  >  3:1480927/1‑145 (MQ=255)                 aTCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTTATCCTTTTTTGGTCACCT‑A‑TCCcaaa                                                                                                                                       >  2:502339/1‑150 (MQ=255)                   cAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAaagaa                                                                                                                                    <  3:896337/151‑1 (MQ=255)                          gACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATTACATTTATTCTTTTTTTGTTACCT‑A‑TCCaaaaaaaaaa                                                                                                                                 >  2:1351668/1‑147 (MQ=255)                            cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTAACATTTATTCTTTTTTTGTTACCC‑A‑TCCCaaaaaaaa                                                                                                                                  >  2:556206/1‑142 (MQ=255)                            cccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑GTATACCATTTATCCTTTTTTTGTTACCT‑A‑TCCaaaaaaaaaa                                                                                                                                 >  2:92567/1‑145 (MQ=255)                             ccTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑GTTTAACCTTTATTCTTTTTTTTTCCTCC‑A‑TCCCAaaaaagaa                                                                                                                                 >  3:441233/1‑144 (MQ=255)                               tGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTATCAATTATTCTTTTTTTTTCTCCT‑ATTCCAaaaaaaaa                                                                                                                                  >  3:1272100/1‑140 (MQ=255)                                ggTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCcgacga                                                                                                                         <  4:2925/149‑1 (MQ=255)                                  tATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATAACATTTATCCTTTTTTTGTTTCCT‑C‑TCCAAAAAAAAAAAacgacggg                                                                                                                        >  2:959721/1‑146 (MQ=255)                                      gTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑GTATAACATTTATTCCTTTTTTGTCACCT‑A‑TCCAAAAAAAAAAAACGGAGAGCT‑a                                                                                                                    >  3:1204715/1‑147 (MQ=255)                                        tACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGc                                                                                                               <  1:7960/150‑1 (MQ=255)                                          cAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCCTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCaaa                                                                                                            <  2:1197695/151‑1 (MQ=255)                                            aaCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAAt                                                                                                          <  3:435239/151‑1 (MQ=255)                                             aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTTCCAATTATTCTTTTTTTGTTACCC‑C‑TCCAAAAAAAAAAAacgacggggccaaagcaaaaa                                                                                                           >  3:1271869/1‑135 (MQ=255)                                             aCGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATAACATTTATTCTTTTTTTTTGACCT‑A‑TCCAAAAAAAAAAAACGAAGAGGT‑aaaaagaaaa                                                                                                           >  3:622624/1‑141 (MQ=255)                                                tAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑ATTTAACATTTTTTTTTTTTTTTTCACCT‑A‑TCCAAAAAAAAAAAGCGGCAAGCC‑AAGAGCAAAAATaat                                                                                                      >  3:972932/1‑149 (MQ=255)                                                 aGAATATGGCCAGGAGTGGTAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTACCATTTATTCTTTTTTTGTTACCT‑C‑TCCCAAAAAAAAAACCGACGAGCT‑AAGAGCAAAAATAAtt                                                                                                     >  2:218630/1‑151 (MQ=255)                                                  gAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTTCCAATTATCCTTTTTTTGTTACCC‑C‑TCCAAAAAAAAAAAACGACGAGCG‑AAAAGGAAAAATAAttt                                                                                                    >  2:299198/1‑150 (MQ=255)                                                  gAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTc                                                                                                    <  2:1457362/151‑1 (MQ=255)                                                      atGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTa                                                                                                <  3:747591/151‑1 (MQ=255)                                                        ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTTATCCTTTTTTTGTCACCC‑A‑TCCCAAAAAAAAAACCGACGAGCT‑AAAAGCAAAAATAATTTGCTaa                                                                                               >  2:979171/1‑150 (MQ=255)                                                        ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAAt                                                                                              <  2:897653/151‑1 (MQ=255)                                                        ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAAt                                                                                              <  2:918767/151‑1 (MQ=255)                                                        ggCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAAt                                                                                              <  3:1043832/151‑1 (MQ=255)                                                          ccAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTAACATTTATTCTTTTTTTGTTACCT‑A‑TCCCAAAAAAAAAAACGACCAGCT‑AAAAGCAAAAATAATTTTCTaa                                                                                               >  2:275471/1‑148 (MQ=255)                                                           cAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAg                                                                                           <  2:936184/151‑1 (MQ=255)                                                             ggAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCACTGATCCCTTTTTTGTTACCTCT‑TCCAAAAAAAAAAG‑CGACGAGCC‑AAAAGGAAAAATAATTTGGTAAtta                                                                                            >  2:1044381/1‑146 (MQ=255)                                                               aGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGt                                                                                       <  3:723559/151‑1 (MQ=255)                                                                 tGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTTATCCCTTTTTTGTCACCT‑A‑TCCCAAAAAAAAAAACGACGAGCC‑AAGAGCAAAATTAGTTTGCTAATAAGACGtt                                                                                      >  2:480733/1‑150 (MQ=255)                                                                 tGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATAACCTTGATCCCTTTTTTGTGACCT‑A‑TCCAAAAAAAAAAGCCGACGAGCT‑AAAAGCAAAAATAGTTCTCTTATCAACCggt                                                                                      >  2:323109/1‑148 (MQ=255)                                                                  ggAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTta                                                                                     <  2:1324831/150‑1 (MQ=255)                                                                    aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTAACATTTATTCTTTTTTTGTGACCT‑A‑TTCAAAAAAAAAAGACGACGGGGC‑AAGAGGAAAAATAATTTGCTAATCAGCCGGtata                                                                                   >  2:1470828/1‑150 (MQ=255)                                                                    aaaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTatat                                                                                  <  2:1553027/151‑1 (MQ=255)                                                                     aaaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtatt                                                                                 <  3:825707/151‑1 (MQ=255)                                                                      aaTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattt                                                                                <  2:1050695/151‑1 (MQ=255)                                                                       aTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTAACATTTATCCTTTTTTTGTCACCC‑A‑TCCCAAAAAAAAAAACGACGAGCT‑AAGAGCAAAAATAATTTGCTAAttaacccgtttattt                                                                                >  2:1308887/1‑136 (MQ=255)                                                                       aTCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattta                                                                               <  2:303284/151‑1 (MQ=255)                                                                           agaaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGCTTTTACAATT‑ATTCTTTTTTTGTCTCCC‑A‑TTCAAAAAAAGAAACCGACGAGCT‑AAGAGCAAAAATAATTTGCTAATCAGACCTTAtatttttttt                                                                           >  3:452428/1‑149 (MQ=255)                                                                           agaaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCAATTATTCTTTTTTTGTTACCT‑A‑TTCAAAAAAAAAAACCGACGAGCT‑AAGAGCAAAAATATTTTGCTTATCAGCCGGTTtatttttttt                                                                           >  2:89123/1‑149 (MQ=255)                                                                             aaagaaGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGttt                                                                         <  2:1355217/151‑1 (MQ=255)                                                                              aagaagATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTa                                                                        <  3:1558513/151‑1 (MQ=255)                                                                                gaagaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTaaa                                                                      <  2:260129/151‑1 (MQ=255)                                                                                   gaTATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTg                                                                   <  3:522988/151‑1 (MQ=255)                                                                                    atatTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑GTTTAACATTTATTCTTTTTTTGTTTCCC‑A‑TCCAAAAAAAAAAAACGGCAAGCTAAAAAGCAAAATTA‑TTTGCTTATTAACCGTTTTATTTTTTTTTTtaa                                                                      >  2:863833/1‑147 (MQ=255)                                                                                           aGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGa                                                          <  3:500351/151‑1 (MQ=255)                                                                                            gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGaa                                                         <  3:1555865/151‑1 (MQ=255)                                                                                                 gATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTTTTCCATTTATCCTTTTTTTTTCACCT‑A‑TCCAAAAAAAAAAAGCGACCCGCC‑AAAAACAAAAATAATTTGCTAAACAACCCTTTTTTTTTTTTTTTTAAATAGC‑ggg                                                             >  3:1191122/1‑141 (MQ=255)                                                                                                  aTGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCCTTGATTCTTTTTTTGTCACCT‑C‑TCCAAAAAAAAAAGACGAAGAGCT‑AAGAGGAAAAATAGTTTTCTTATAAGCCGCTTTATTTTTTTTTTTAAATGGC‑GGGTTTAttgttt                                                   >  2:1334908/1‑150 (MQ=255)                                                                                                  aTGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAAttgttg                                                   <  3:585835/151‑1 (MQ=255)                                                                                                              ggggATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGcc                                       <  3:1060351/151‑1 (MQ=255)                                                                                                              ggggATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGcc                                       <  2:485387/151‑1 (MQ=255)                                                                                                                    aaaaTTTACGGGGT‑TTATCCTTTTGTTCTTTTATGGCTCACCT‑A‑CCAAAAAAAAGAAGCCGACGAGAA‑AAGAGAAAAATTAGTTCCCTAACCGGCTTTTATATTTTTTGTTAAACCGGGC‑GGTGAATTTGTTGTCTTCAACCGCCATgag                                  <  3:1072191/150‑1 (MQ=255)                                                                                                                        tttACGGGGT‑TTATACTTTTGATCTTTTATTGGTCCCCT‑A‑CCAAAAAAAAGAACCCGACGAGTA‑AAGAGAAAAATTATTCCGCTAACCCGCCGTTATATTTATTGTTAAAACGGGG‑GTTTAAATTGTTGTCTTCAACCGCCATGAGAAac                              <  3:635049/150‑1 (MQ=255)                                                                                                                            cggcgg‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGGTTGATTTTTTTTTTTTAACccccttaggaaaacac                           >  2:216116/1‑138 (MQ=255)                                                                                                                             ggggg‑TTATACCATTGATCTTTTATTGGTCCCCT‑A‑TCAAAAAGAAGAAGCCGACGAGTT‑AAGAGAAAAATTAGTTCGCTAATCAGCCGTTATTTTTATTTTAAAAACGGGG‑GGTTAAATTGTTTTCTCAAACCGCCATGAGAAactcactc                        <  3:91615/148‑1 (MQ=255)                                                                                                                              gcgt‑TTATACCTTTGATCTTTTATTGGTCCCCT‑A‑CCAAAAAAAAGAGGCCGACGAGCT‑AAGAGAAAAATTAGTTCGTTAACCAGCCTTTATATTTATTTTTAAACCTGGC‑GTTTAATTTGTTGTCTTCAACCGCCATGAGAAACTCActct                       <  3:551655/151‑1 (MQ=255)                                                                                                                               gggtttatccttttaaTCTTTTTTTGGCCACCT‑A‑CCCAAAAGAAGAGCCCGACGAGAT‑AAAAGAAAAATTAGTCCGCTAATCGGCCTTTTTTTTTATTTTTTAAACGGGC‑TTTTGAATTGTTGTCTCCAACCGCCATGAGAAACTCActct                       <  3:1047034/136‑1 (MQ=255)                                                                                                                                ggtttATACTTTTGTTCTTTTTTTGGTCACTT‑C‑TCAAAAAAAAGAGCCCGACGAGCT‑AAAAAAAAATTTTTTTCTTTACTCACCCATAATATTTTTTGTTAAAGCTGGC‑TGTTGATTTGTTGTTTTCAACCGCCATGAGAAACTCActct                       <  3:1491439/147‑1 (MQ=255)                                                                                                                                gg‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTTAATTGTTTTTTTCCACCGCCATGTGaaa                               >  3:565696/1‑141 (MQ=255)                                                                                                                                         cATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCcac                 >  2:1285770/1‑147 (MQ=255)                                                                                                                                             gATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTTTCTTCCACCGCCATGAGAAAATCACTCTGCCCACAGCGcc           >  3:714692/1‑149 (MQ=255)                                                                                                                                                  tttATTGTTCCCCT‑A‑CCAAAAAAAAAACGCCGAGGAGAA‑AAGAAAAAAATTATTTCGCAAACCACCCTTTATTTTTTTTTTTAAACCTGGC‑GTTGGATTTTTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGt        <  3:862280/147‑1 (MQ=255)                                                                                                                                                  tttATTGGTCCCCT‑A‑CCCAAAAGAAGAAGCCGACGAGCT‑AAGAGAAAAATTATTTCGTTAATCAGCCGTTATTTTTTTTTTTTAAACTGGG‑GGTTGATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGc      <  2:1424398/149‑1 (MQ=255)                                                                                                                                                    tATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTTTCTTCAACCCCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCaa   >  3:1422916/1‑150 (MQ=255)                                                                                                                                                    tATTGGCCCCCT‑A‑CCAAAAAAAAAAAGCCGACGAGCA‑AAGAGCAAAATTAGTTCGCTAATCAGCTTTTATATTTATTTTTAAAACGGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa   <  2:1453743/150‑1 (MQ=255)                                                                                                                                                     aTTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGTATTGTTGTCTTCAACCTCCATGAGAAACACACCCTGCCCACAGCGCCAGTGCCAAc  >  3:857587/1‑150 (MQ=255)                                                                                                                                                     aTTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGCTTAATATTTTGCTTTAAACGCCCTTAGAAAATCACTCTGCCCACCGCGCGATTGCTaa   >  3:663798/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                          CATGTTTATTGGCGTAATCAGCAATGACCCTGGTATTGTTACAAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCT‑A‑TCCAAAAGAAGAAGCCGACGAGCT‑AAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGC‑GGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAC  >  NC_000913/2151442‑2151738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑