Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,511,9230AC41.8% 217.1 / 12.4 110V141G (GTT→GGT) yhfZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (54/10);  new base C (0/46);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 1.61e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

ACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTG  >  NC_000913/3511775‑3512073
                                                                                                                                                      |                                                                                                                                                      
aCGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAacc                                                                                                                                                      >  2:563817/1‑151 (MQ=255)
aCGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAacc                                                                                                                                                      >  2:501603/1‑151 (MQ=255)
aCGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAacc                                                                                                                                                      <  2:1484574/151‑1 (MQ=255)
 cGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAacc                                                                                                                                                      >  3:1494868/1‑150 (MQ=255)
 cGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAacc                                                                                                                                                      >  2:778297/1‑150 (MQ=255)
              ccACGCGCTTCACTTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATa                                                                                                                                        <  2:1426878/151‑1 (MQ=255)
                  gcgcTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCTTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATacac                                                                                                                                     <  3:1140623/150‑1 (MQ=255)
                  gcgcTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACg                                                                                                                                    >  2:203113/1‑151 (MQ=255)
                    gcTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGcc                                                                                                                                  >  2:1534353/1‑151 (MQ=255)
                      ttCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCAt                                                                                                                                >  2:66734/1‑151 (MQ=255)
                      ttCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCAt                                                                                                                                >  3:67882/1‑151 (MQ=255)
                       tCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCAtt                                                                                                                               >  3:541439/1‑151 (MQ=255)
                        cACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCAtt                                                                                                                               >  3:282532/1‑150 (MQ=255)
                           gTTTGGGGACTCGCTTTTCCGGCAAATCAACGGGTGCCCGCCAACGTGGGTGGGGGGCCCCACCCCCA‑GCGCGAGGCAAAAGCTTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAg                                                                                                                            <  2:282552/150‑1 (MQ=255)
                             tggCGGACTCGCTTTTACGGCAAATCAACGGGTGCTCGCCAACGTGGGTGTGCGGCCCCAACCCCA‑GCGCGGGGCAAAAGCTTTTTTGCGGGAGATAATTTC‑CCCCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCa                                                                                                                          <  2:1092916/148‑1 (MQ=255)
                               gCGGCCCCGCTTTTCCGGCAAATCAACTGGTGCTCGCAACCGGGGGTGGGCGCCCCCAACCCCA‑GCGGGGGGCAAAACCTTTTTTGGGTGAGATAACTTT‑CCGCCGCTAGGCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAg                                                                                                                         <  3:171467/149‑1 (MQ=255)
                                 ggccTCGCTTTTGCGGCAAAGCAATTGGTGCTCGCCACCGTGGGTGTGCGCCCCCAACTCCA‑GCGGGGGGCATAAGCCTTTTTGCGTGAAATAACTTC‑CCGCCGTTATTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAg                                                                                                                         <  2:1215658/144‑1 (MQ=255)
                                    cTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTcc                                                                                                                  >  2:1355670/1‑151 (MQ=255)
                                     tCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTcc                                                                                                                  >  3:1531115/1‑150 (MQ=255)
                                      cGCTTTTACGGCAAACCAACGGGGCCTGGCCAACGTGGGTGTGCGCCCCAACCCCCA‑GCGGGGGCAAAAACCCTTTTTGGGGGAGATATTTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCAc                                                                                                                <  3:1361381/151‑1 (MQ=255)
                                        cttttCCGGCAAACCACCGGGTGCTCGCCACGGTGGGTGTGCGCCCCCACCCCCA‑GCGGGGGGCAAAAGCTTTTTTGCGTGAGATACCTTT‑CCGCCGCTGGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTcc                                                                                                                  <  2:815017/145‑1 (MQ=255)
                                              cGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAatat                                                                                                        >  2:232670/1‑151 (MQ=255)
                                                 cAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATcc                                                                                                      >  3:170932/1‑150 (MQ=255)
                                                  aaataacttGGTGCTCGCCAAGGTGGGTGTGCGCCCCCACCCCCA‑GCGCGGGCCATAACCTTTTTTGCGTGAAATACCTTC‑CCGCCGCTAGCCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAatat                                                                                                        <  3:1188573/139‑1 (MQ=255)
                                                  aaaTCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCAAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCcg                                                                                                     <  2:532887/150‑1 (MQ=255)
                                                  aaaCCAACTGGTCCTCGCCAACGTAGGTGTGCGCCCCCAACCCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTC‑CCGCCGCTGGTCGCGAACCCCCCGCCATGTCATACACGCCATTAATCAGACACTCCACGCGAATATCcgc                                                                                                    <  3:466009/151‑1 (MQ=255)
                                                       aaCTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCAc                                                                                               >  3:32643/1‑151 (MQ=255)
                                                          tGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGc                                                                                             >  2:1056214/1‑150 (MQ=255)
                                                          tGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGc                                                                                             >  2:1446778/1‑150 (MQ=255)
                                                           ggtccccGCCACCGTGGGTGGGCGCCCCCACCCCCG‑GCGCGGGCCAAACGCTTTTTGGGGTGAGAAACCTTC‑CCGCCGCTAGCGGGGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACg                                                                                              <  3:943148/142‑1 (MQ=255)
                                                           ggTGCTCGCCACCGTGGGTGGGCGCCCCCACCCCCG‑GCGCGGGGAAAAACCCTTTTTCCGTGAAATAACTTC‑CCGCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCa                                                                                            <  3:1414282/150‑1 (MQ=255)
                                                                tCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTgcg                                                                                      >  2:394569/1‑151 (MQ=255)
                                                                tCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTgcg                                                                                      >  2:1486187/1‑151 (MQ=255)
                                                                 cccaaaCGTAGGTGTCCGCCCCCACCCCCA‑GCGCGGGCCATAACCTTTTTTGCGTGA‑AAAACTTTCCCCCCGCTGGCCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTgcg                                                                                      <  3:632831/146‑1 (MQ=255)
                                                                 cGCCAACTTGGGTGTGCGCCCCCACCCCCG‑GCGGGGGGCAAAACCTTTTTTGGGTGAAATAACTTC‑CCGCCGCTAGTCGCAAAACCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTgc                                                                                       <  3:1062013/149‑1 (MQ=255)
                                                                  gCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTgcgc                                                                                     >  3:624223/1‑150 (MQ=255)
                                                                   ccAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCTTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCAt                                                                                   <  2:1133110/151‑1 (MQ=255)
                                                                       cGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGt                                                                                >  2:1015341/1‑150 (MQ=255)
                                                                        gTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTa                                                                               >  3:1630377/1‑150 (MQ=255)
                                                                          aGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTaaa                                                                             >  3:1484602/1‑150 (MQ=255)
                                                                           ggTGTGCGGCCCCACCCCCA‑GCGCGGGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTaaa                                                                             <  2:539734/149‑1 (MQ=255)
                                                                           ggTGTGCGGCCCCACCCCCA‑GCGCGAGGAATAAGCCTTTTTGCGTGAAATACCTTT‑CCCCCGCTGGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTaa                                                                              <  2:853422/148‑1 (MQ=255)
                                                                           ggTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTaa                                                                              <  2:265515/148‑1 (MQ=255)
                                                                            ggtggccgCCCCAACCCCAGGCGCGGGCAAAAAGCTTTTTTGCGTGA‑AAAACTTTCCCGCCGCTGGCCGCGAACCCACCGCCAGGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTa                                                                               <  3:1224997/140‑1 (MQ=255)
                                                                                gcgcCCCCACCTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATACCTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGa                                                                        <  2:1601102/149‑1 (MQ=255)
                                                                                 cGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATg                                                                     >  3:973169/1‑151 (MQ=255)
                                                                                   gCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGCCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGcc                                                                   >  3:1151298/1‑151 (MQ=255)
                                                                                    ccccACCTCCA‑GCGCGAGGCATAAGCTTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCa                                                                  <  2:618129/151‑1 (MQ=255)
                                                                                        acccccA‑GCGCGAGCCAAAACCTTTTTTGGGGGAAAAACCTTC‑CCCCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCaa                                                              <  3:6353/147‑1 (MQ=255)
                                                                                          ccccA‑GCGCGGGGCAAAACCTTTTTGGCGTGAAAAACCTTC‑CCGCCGCTAGCCGAAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCaaa                                                             <  3:1005204/148‑1 (MQ=255)
                                                                                          ccccA‑GCGCGAGGCATAAGCTTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAAc                                                            <  2:701943/149‑1 (MQ=255)
                                                                                          ccccA‑GCGCGAGGCATAAGCTTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCaaa                                                             <  2:219401/148‑1 (MQ=255)
                                                                                          cTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAAc                                                            >  2:593898/1‑151 (MQ=255)
                                                                                           tCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACt                                                           >  2:405707/1‑151 (MQ=255)
                                                                                           tCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACt                                                           >  3:278653/1‑151 (MQ=255)
                                                                                           tCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACt                                                           >  2:1327862/1‑151 (MQ=255)
                                                                                            ccA‑GCGGGGGCCAAAAGCCTTTTTGCGTGAAAAAACTTC‑CCGCCCCAAGTCGGGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCaaa                                                             <  3:1562418/148‑1 (MQ=255)
                                                                                                  gcgAGGCATAACCTTTTTTGCTTGAGATACCTTC‑CCGCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTgg                                                         <  3:725206/147‑1 (MQ=255)
                                                                                                   cgAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCttt                                                    >  2:1395688/1‑151 (MQ=255)
                                                                                                    ggggCATAACCTTTTTGGCGTGAAATAACTTC‑CCGCCGCTAGTCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTggg                                                        <  2:1270723/144‑1 (MQ=255)
                                                                                                     gggaaaaaGCTTTTTTGCGTG‑GAAAACTTTCCCCCCGCTAGTCGCAAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCa                                                  <  3:71876/145‑1 (MQ=255)
                                                                                                      ggCATAAGCTTTTTTGCGTGAGATAACTTC‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCaa                                                 <  2:1116755/151‑1 (MQ=255)
                                                                                                      ggCATAAGCTTTTTTGCGGGAGATACCTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCttt                                                    <  2:641176/148‑1 (MQ=255)
                                                                                                      ggCATAAGCTTTTTGGCGTAAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCa                                                  <  2:174696/150‑1 (MQ=255)
                                                                                                         aTAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATcc                                              >  2:1571414/1‑151 (MQ=255)
                                                                                                          tAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCg                                             >  2:1177982/1‑151 (MQ=255)
                                                                                                           accccTTTTTGCGTGAGATACCTTC‑CCGCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCg                                             <  2:42759/147‑1 (MQ=255)
                                                                                                           aacccTTTTGGCGTGAGAAAACTTC‑CCCCCGCTAGCCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATcc                                              <  3:1637336/146‑1 (MQ=255)
                                                                                                            aGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCt                                           >  3:541724/1‑151 (MQ=255)
                                                                                                              cttttttGGGTGAGATACCTTC‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTc                                          <  2:1590064/148‑1 (MQ=255)
                                                                                                              cttttttGCGGGAGATAACTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTc                                          <  3:1505212/148‑1 (MQ=255)
                                                                                                              ccTTTTTGCGTGAGATACCTTC‑CCCCCGCTAGTCGGAAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCt                                           <  2:1116680/149‑1 (MQ=255)
                                                                                                              ccTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCg                                         >  3:45888/1‑151 (MQ=255)
                                                                                                               cTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGc                                        >  2:753060/1‑151 (MQ=255)
                                                                                                               cTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGc                                        >  2:1193006/1‑151 (MQ=255)
                                                                                                                tttttGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGcc                                       >  2:1646594/1‑151 (MQ=255)
                                                                                                                tttttGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGcc                                       >  3:23582/1‑151 (MQ=255)
                                                                                                                  tttGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGcc                                       >  2:342182/1‑149 (MQ=255)
                                                                                                                     gggTGAAATACCTTT‑CCGCCGCTGGTCGCAAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAg                                     <  3:270295/146‑1 (MQ=255)
                                                                                                                     gCGTGAGATACCTTC‑CCGCCGCTAGTCGCGAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGc                                   <  2:315816/150‑1 (MQ=255)
                                                                                                                      tgTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCCCCCTGGGCTTTCCCTGCGCTCGCCCGGCCt                                 >  3:393784/2‑151 (MQ=255)
                                                                                                                        tGAAATACCTTC‑CCGCCGCTAGTCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCtt                                <  2:595259/150‑1 (MQ=255)
                                                                                                                             tAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTAc                           >  2:310531/1‑150 (MQ=255)
                                                                                                                              aatttt‑CCGCCCCTAGCCGCGAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTa                            <  2:360811/145‑1 (MQ=255)
                                                                                                                               aCTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACaa                         >  2:656239/1‑150 (MQ=255)
                                                                                                                                 ttt‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACg                      >  3:1060193/1‑151 (MQ=255)
                                                                                                                                  ttccccccgcTAGCCGCAAACCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTa                            <  3:887667/139‑1 (MQ=255)
                                                                                                                                   t‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACggg                    >  2:426172/1‑151 (MQ=255)
                                                                                                                                   t‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACggg                    >  2:896751/1‑151 (MQ=255)
                                                                                                                                      ccccgcTGGCCGCAAAACCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGta                  <  2:1118481/149‑1 (MQ=255)
                                                                                                                                        cgcctggTCGGGAACCCCCCGCCATGTCATCCACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGtat                 <  3:11260/144‑1 (MQ=255)
                                                                                                                                         cgcTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGtat                 >  3:1592882/1‑149 (MQ=255)
                                                                                                                                            tAGTCGCAAACCCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagg              <  2:858718/149‑1 (MQ=255)
                                                                                                                                               tCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggca           >  2:823057/1‑149 (MQ=255)
                                                                                                                                               tCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggca           >  2:405799/1‑149 (MQ=255)
                                                                                                                                                cgcgAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggcaagg        >  2:959047/1‑151 (MQ=255)
                                                                                                                                                  caaaaCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggcaagg        <  3:398973/147‑1 (MQ=255)
                                                                                                                                                   gAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggcaagggc      >  3:1355164/1‑150 (MQ=255)
                                                                                                                                                    aaaCCCCCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATagggcaagg        <  2:967186/147‑1 (MQ=255)
                                                                                                                                                      accaccGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCTTTCGTACAAACGGGTATAGGGCAAGGGCATTg  >  2:1134592/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
ACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA‑GCGCGAGGCATAAGCCTTTTTGCGTGAGATAACTTT‑CCGCCGCTAGTCGCGAAACCACCGCCATGTCATACACGCCATTAAGCAGACACTCCACGCGAATATCCGCGCCACGCATGTGCGCATAGTAAAAAGGAATGCCATCAAACTGGGCTTTCAATCCGCTCGCCAGGCCTTCGTACAAACGGGTATAGGGCAAGGGCATTG  >  NC_000913/3511775‑3512073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑