Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913345,3280CA46.1% 142.3 / 58.3 102intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (50/5);  new base A (0/47);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 8.94e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTG  >  NC_000913/345179‑345475
                                                                                                                                                        |                                                                                                                                                    
aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa                                                                                                                                                     <  2:321770/151‑1 (MQ=255)
 aaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCa                                                                                                                                                     >  3:1646248/1‑150 (MQ=255)
  aaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaa                                                                                                                                                   <  2:577064/151‑1 (MQ=255)
  aaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaa                                                                                                                                                    >  2:367949/1‑150 (MQ=255)
    aaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCaaaaa                                                                                                                                                 <  3:106162/151‑1 (MQ=255)
      tGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAAcc                                                                                                                                              >  2:1051349/1‑151 (MQ=255)
      tGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAAcc                                                                                                                                              >  3:1338595/1‑151 (MQ=255)
          ctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGcc                                                                                                                                           >  3:769812/1‑150 (MQ=255)
           tcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCAt                                                                                                                                         >  3:1515629/1‑151 (MQ=255)
            cATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCAtt                                                                                                                                        >  3:75111/1‑151 (MQ=255)
               gCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGc                                                                                                                                     >  3:809207/1‑151 (MQ=255)
                cAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGc                                                                                                                                     >  3:1560711/1‑150 (MQ=255)
                    tGTTTTGTGAATGTTTTGTTTGACATTCATATAAAAAAAATCATAATCCCATCTTGTTTGTGGGGGGTTTTTTCTGTATCTTCCGCACCGCACTAAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCt                                                                                                                                    <  2:1108603/147‑1 (MQ=255)
                      ttttGTGAATGTGTTGTTTGACATTCATATAAAAAAATTCATAATCCCATCATGTTTGTGTGGGTTTTTTTCTGTATCTTACGCATCCCACTCAAGCCTGACAAAAAATAGGATTAAAGGCTAATATAAAAAACCGCCATTAGCtcatca                                                                                                                               <  3:1236722/150‑1 (MQ=255)
                       tttGTGAATGTTTTGGTTGACATTCATAGGAAAAAAATCATAATCCCATCATTTTTGTGGGGGTTTTTTTCTGTATCTTACGCATCGCACCAAGGCCTGACAAAAAATATGCTGTAGGCTTAATATAAAAAACCGCCATTAGCTCATCAg                                                                                                                              <  2:1169995/150‑1 (MQ=255)
                        ttGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAgg                                                                                                                             >  2:962742/1‑150 (MQ=255)
                         tgtgATTGTGTTGTTTGACTTTAATATAAAAAAAATCATAATCCCATCATTTTTGGGTGGGGTTTTTTCTGTTTCTTACCAACCGCACCCAACCCTAAAAAAAAATAGGATGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGa                                                                                                                            <  3:622582/150‑1 (MQ=255)
                          gtgAATGTGTTGGTTGAAATTCATAAAAAAAAAATTATAATCCCATCATGTTGGTGTGGGTTTTTTTCTGTATCTAACGCACCGCCCCCACCCCTGAAAAAAAATAGGCTGTAAGGCAAATAAAAAAAACCGCCATTAGCTCATCAGGaa                                                                                                                           <  3:582418/150‑1 (MQ=255)
                          gtaattgtttgggttaACATTCATATAAAAAAAATAAAAATCCCATCATGTTGGTGGGGGTTTTTTTCTTTATCTTCCCCACCCCCCCCAAGCCGAACAAAAAATATGCTGAAAGGCTAAAATAAAAATCCGCCATTAGCTCATCAGGaa                                                                                                                           <  3:516067/139‑1 (MQ=255)
                            gAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAag                                                                                                                          >  2:493543/1‑149 (MQ=255)
                               tgtttggtttgACTTTAATAAAAAAAAAATCATAACTCCATCTTTTTTGTGTGGTTTTTTTTTTTTATCTTACCCCCCCCACAAAAGCCAGAAAAAAAATATTCTATAGGGCACATATAAAAACCCGCCATTAGCTCATCAGGaa                                                                                                                           <  3:1459407/137‑1 (MQ=255)
                               tgtgTTGTTTGACTTTAATAAAAAAAAAATCAAAATCCCATAATTTTTGTGTGGGTTTTTTTTTGTATCTTACGCCCCCCACCCAACCCTGAAAAAAAATATGCTGAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAaga                                                                                                                         <  3:1488909/147‑1 (MQ=255)
                               tgtgTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                       >  2:788073/1‑149 (MQ=255)
                               tgtgTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                       >  2:1073890/1‑149 (MQ=255)
                                 ttttgtttgACATTCATATAAAAAAAATCATAATCCCATCATGTTTGTGTGGGGTTTTTTCTGTTTTTTCCGCATCGCACCCAAGCCTAAAAAAAAATTTGCTGAAGGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAg                                                                                                                     <  2:1164418/147‑1 (MQ=255)
                                 tgtTGGTTGACATTCATATAAAAAAAATCATAATCCCATCATGTTTGTGTGGGGTTTTTTCTGTATCTTACGCATCGCACTAAACCCTGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAga                                                                                                                    <  2:566625/150‑1 (MQ=255)
                                  gttggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  >  2:492942/1‑151 (MQ=255)
                                   ttggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacg                                                                                                                  >  2:1436769/1‑150 (MQ=255)
                                   ttggttgACATTAATATAAAAAAAATAATAATCCCATCATGTTTGTGGGGGTTTTTTTTTTTATCTTACGCACCCCACCAAAGCCTGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                   <  2:1216429/149‑1 (MQ=255)
                                   ttggttgACATTAATAAAAAAAAAATAATAATTCCATCTTGTTTGGGGGGGTTTTTTTTTTTATCTTACGCACCCCCCCAACGCCTGAAAAAAAATATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                   <  3:913979/149‑1 (MQ=255)
                                   ttggttgAAATAAATATAAAAAAAATATTATTACCATCTTGTTGGTGTGGGTTCTTTTCTTTATGGTACCCTTCGCACACATGCCTAGAAAAAAATTTTCGCAAGGTCTCAATCAAAAATCCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                   <  3:878983/149‑1 (MQ=255)
                                     ggttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac                                                                                                                >  2:168979/1‑150 (MQ=255)
                                      gttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACa                                                                                                               >  3:1102172/1‑150 (MQ=255)
                                       ttgTCATTCTTAAAAAAAAAAATATACTCCCTCCATGTTTGTGTGGGGTCTTTTCTTTATCTTCCCCTTCGCCCGCAAGCCTCACAAAAAATGTGGTATAAGCCTAATAACAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                   <  2:1254339/145‑1 (MQ=255)
                                        ttcatattcataaaaaaaaaaaaaaaaTCCATAATTGTTTGTGTGGGGTTTTTTCTTTTCTTTACCAATCGCACTCACCCCGGACAGAAAATATGTTTAAAGGCTAAAATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACaa                                                                                                              <  2:1184423/137‑1 (MQ=255)
                                             ttaatatAAAAAAAATAATAATCCCATCTTGTTGGTGGGGGTTTTTTTTTGTTTTTACCCCACCCCCCACAAGCCAGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCa                                                                                                           <  3:318812/144‑1 (MQ=255)
                                                atatGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgt                                                                                                    >  3:869840/1‑151 (MQ=255)
                                                atatAAAAAAAATCTTAATCCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt                                                                                                       <  2:315683/148‑1 (MQ=255)
                                                  atGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAttgttg                                                                                                  >  2:1317518/1‑151 (MQ=255)
                                                     aaaaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTAt                                                                                               >  2:1522425/1‑151 (MQ=255)
                                                       aaaaaTCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATgg                                                                                             >  2:48742/1‑151 (MQ=255)
                                                               tAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGt                                                                                     >  3:1413072/1‑151 (MQ=255)
                                                                aaTCCCATCTTTTTGGGGGGGGGTTTTTTTTTTTTCTTCCGCTCCCCCCTAAAGCCGGAAAAAAAATATGTTGAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACggg                                                                                       <  3:1236973/148‑1 (MQ=255)
                                                                aaTCCCATCATTTTGTGGGGGGTTTTTTTTTTTATTTTACGCACCGCCCTAAACCCTAAAAAAAAATATGCTTAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGt                                                                                     <  2:832736/150‑1 (MQ=255)
                                                                  ttCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                  >  2:1368051/1‑151 (MQ=255)
                                                                   tCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                  >  3:226736/1‑150 (MQ=255)
                                                                    cccttcttGTTTTGGTGGGTTTTTTTCTGTTTCTTCCGCGCCCCACTCAAGCCTAAAAGAAAAGATGATTTAGGCCTCAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                  <  3:651524/143‑1 (MQ=255)
                                                                    ccATCTTGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                    <  2:31146/147‑1 (MQ=255)
                                                                    ccATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                  >  2:892635/1‑149 (MQ=255)
                                                                       tcatTTTTGTGGGGGTTTTTTTTTTTATCTTACGCACCGCACAAAAGCTGAAAAAAAAATAGGCTGAAAGGTTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGa                                                                                 <  3:1613900/147‑1 (MQ=255)
                                                                       tcatGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGcc                                                                             >  2:596235/1‑151 (MQ=255)
                                                                       tcatGTTGGTGTGGGTTTTTTTCTGTATCTTCCCCATCGCACCCAACCCTGAAAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAg                                                                                <  3:418039/148‑1 (MQ=255)
                                                                         atGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc                                                                           >  3:295181/1‑151 (MQ=255)
                                                                           tGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg                                                                          >  3:529087/1‑151 (MQ=255)
                                                                            gTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg                                                                          >  3:916086/1‑150 (MQ=255)
                                                                            gTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCg                                                                          >  2:268101/1‑150 (MQ=255)
                                                                                  gtgGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCgg                                                                   >  3:588602/1‑151 (MQ=255)
                                                                                   gggggtttttttctttttctTACGCACCCCCCTCAAGCCTGAAAAAAAATATGCTAAAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCgg                                                                   <  3:909223/143‑1 (MQ=255)
                                                                                    ggggTTTTTTCTTTATCTTACGCACCGCCCACAAGCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGt                                                                  <  3:1395898/150‑1 (MQ=255)
                                                                                    ggggTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTc                                                                 >  3:515115/1‑151 (MQ=255)
                                                                                         tCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAAc                                                             >  2:70231/1‑151 (MQ=255)
                                                                                           ttttCTTTATCTTACCCACCCCACTCAACCCTGAAAAAAAATATGCTGAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAAc                                                             <  2:729848/149‑1 (MQ=255)
                                                                                           ttttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtt                                                           >  3:157920/1‑151 (MQ=255)
                                                                                            tttgtttttctcccGCATCGCACACAGGCCTGAAAAAAAATATGTTGAAGGTCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtt                                                           <  3:1461268/137‑1 (MQ=255)
                                                                                             ttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtttt                                                         >  2:542887/1‑151 (MQ=255)
                                                                                                tttATCTTACCCATCGCACACAACCTTGACAAAAAATATGTTGAAAGCCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCa                                                       <  3:1173339/148‑1 (MQ=255)
                                                                                                tGTTTCTTCCGCATCCCACTCAAGCCTAAAAAAAAATATGCTGTAAGCCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTc                                                        <  2:198500/149‑1 (MQ=255)
                                                                                                tGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCa                                                       >  3:1218262/1‑150 (MQ=255)
                                                                                                       tACGCACCGCACTAAAGCCTGAAAAAAAATATGCTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaaa                                                    <  2:723639/146‑1 (MQ=255)
                                                                                                         cgcaccgcacTCAAGCCTGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtctt                                               <  2:111229/149‑1 (MQ=255)
                                                                                                         cGCATCCCACTCAAGCCGGAAAAAAAATATGCTGTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtcttct                                             <  2:1219840/151‑1 (MQ=255)
                                                                                                            aTCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGa                                           >  2:907223/1‑150 (MQ=255)
                                                                                                              cccacacaAGCCGGAAAGAAAATATGCTGAAGGCCTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAAt                                         <  3:1612108/148‑1 (MQ=255)
                                                                                                               gCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                        >  2:1456530/1‑150 (MQ=255)
                                                                                                                caccaaacccggACAAAAAATATCCTTAAAGCCTAATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtcttc                                              <  2:1237615/132‑1 (MQ=255)
                                                                                                                 aCTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                        >  3:1078859/1‑148 (MQ=255)
                                                                                                                  cacaccccTGACAAAAAATATGTTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCa                                       <  3:244215/142‑1 (MQ=255)
                                                                                                                  cTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                        >  2:200340/1‑147 (MQ=255)
                                                                                                                     agccctaaaaGAAAATATGGTAAAAGCTTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCAGTCCAACTTTTCAAAACTCTTCTGAATCAGa                                     <  3:916243/138‑1 (MQ=255)
                                                                                                                     aaGCCTAAAAAAAAATTTGTTGTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCa                                       <  2:809254/145‑1 (MQ=255)
                                                                                                                      aGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcccc                                >  3:494652/1‑151 (MQ=255)
                                                                                                                      aGCCTAACAAAAAATATGCTGTAGGGCTCAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAg                                      <  2:786433/145‑1 (MQ=255)
                                                                                                                        ccgaaaaaaaaaTATGCTTTAAGGCTAAAATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATcc                                  <  3:1307211/139‑1 (MQ=255)
                                                                                                                        ccTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt                              >  2:17600/1‑151 (MQ=255)
                                                                                                                        ccTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt                              >  2:704284/1‑151 (MQ=255)
                                                                                                                            aaaaaaaaTATGCTGAAGGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCttatta                          <  3:1279731/147‑1 (MQ=255)
                                                                                                                              aaaaaaTATGCTGAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtta                             <  2:144277/144‑1 (MQ=255)
                                                                                                                              aaaaaaTATGCTGAAAGGCTAATATAAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                        <  3:1603667/149‑1 (MQ=255)
                                                                                                                              aGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                        >  3:415270/1‑151 (MQ=255)
                                                                                                                                 aaaTATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcatc                     >  3:1601820/1‑151 (MQ=255)
                                                                                                                                       gcggTAAGGCTAATATAAAAAACCGCCATTAGCTTATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCt                  <  2:1346739/145‑1 (MQ=255)
                                                                                                                                         tGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaa              >  2:958966/1‑150 (MQ=255)
                                                                                                                                            aaGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTg          >  2:63403/1‑151 (MQ=255)
                                                                                                                                            aaGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCaaa             <  2:1047530/148‑1 (MQ=255)
                                                                                                                                            aaGGCTCATAAAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATccc                   <  3:1625818/142‑1 (MQ=255)
                                                                                                                                              ggCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa        <  2:919733/151‑1 (MQ=255)
                                                                                                                                              ggCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGa        <  2:785820/151‑1 (MQ=255)
                                                                                                                                              ggCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGt           <  3:1176957/148‑1 (MQ=255)
                                                                                                                                               gCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGaa       <  3:712854/151‑1 (MQ=255)
                                                                                                                                                 cTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAAc      >  2:1608674/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                    
AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGGTCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCTGTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATTG  >  NC_000913/345179‑345475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: