Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,890	0	C	A	46.4%	55.5 / 44.4	71	L267F (TTG→TTT)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (25/12);  new base A (0/32);  total (25/46)
Fisher's exact test for biased strand distribution p-value = 5.41e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA  >  NC_000913/357741‑358035                                                                                                                                                           |                                                                                                                                                    tGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaac                                                                                                                                                     >  3:490141/1‑150 (MQ=255)     aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc                                                                                                                                                <  3:1425133/151‑1 (MQ=255)      aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                                               <  2:896948/151‑1 (MQ=255)      aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                                               <  2:1446198/151‑1 (MQ=255)       gCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc                                                                                                                                               <  2:1232859/150‑1 (MQ=255)         gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc                                                                                                                                            <  3:24959/151‑1 (MQ=255)             ccaataaGGCATTCCCAGCCGCAGCCTTGTGTCAGCGGCTACCGTGA‑TTCATTCCCGCC‑AA‑CACCCGCGCATTTATCCAACGCCTTGTGAAAAAAT‑CCCTCC‑GGGGCGGCTGTCTCCCAGCTTTTTTTCCGCCGCAACAAAAccgccg                                                                                                                                           <  3:976774/142‑1 (MQ=18)                 tCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtctctc                                                                                                                                    >  2:1422117/1‑151 (MQ=255)                   aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                                   >  2:1591011/1‑150 (MQ=255)                   aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                                   >  3:303283/1‑150 (MQ=255)                     gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                >  2:115315/1‑151 (MQ=255)                     gCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                >  3:678947/1‑151 (MQ=255)                       aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                >  3:1626660/1‑149 (MQ=255)                        aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                             >  2:619217/1‑151 (MQ=255)                        aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt                                                                                                                             >  2:752390/1‑151 (MQ=255)                         ttCCAGCCGCAGCCCTGTGTCAGCGGCTCCCGTGA‑TTCATTCCCGCC‑AA‑CACCCGCGCTTTTACCCAACGCCATGTGAAAAAAT‑GCCTTC‑GCGGCGGCTGTCTCCCATTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCagtagt                                                                                                                             <  2:394864/150‑1 (MQ=18)                         ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                            >  3:536020/1‑151 (MQ=255)                         ttCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg                                                                                                                            >  3:1270600/1‑151 (MQ=255)                              gCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggg                                                                                                                       >  3:636234/1‑151 (MQ=255)                               ccGCAGCCTTGTTCCAGCGCCTCCCGTG‑‑TTCCTTTCCCCCAAA‑CACCCGCGCTTTTACCCACCGCAGTGTGAAAAAAT‑GCCTTC‑GGGGGGGCTGTCTGCCGGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      <  3:70444/151‑1 (MQ=255)                               ccGCAGCCTTGTGTCGGGGCCTCCCGTTA‑TTCTTCCCCCCC‑AA‑CACCCCCTCTTTTCCCCACCGCCTTGCAAAAAAAT‑GCCTTC‑GGGGGGTCTGTCTGCCTGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      <  3:165028/151‑1 (MQ=255)                               ccGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      >  2:94718/1‑151 (MQ=255)                                cGCAGCCTTGTGCCGGCGGCT‑CCGTTA‑TTTCTTTCCCCC‑AACCACCCGCGCTTTTCCCAACCCCCTTTTAAAAAAAT‑GCTTCC‑GCGGCGGTTGTCGCCCACTTTTTTTCCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                                     <  2:475221/151‑1 (MQ=255)                                cGCAAACCTGTGGCAGCGCCT‑CCGTTATTTCTTCCCCGCA‑AA‑CAACCGCCCTTTTTCCCACCGCCTTGTCCAAAAGT‑GCTTCG‑GGGGCGGCTGTGTGCCGGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc                                                                                                                     <  3:71883/151‑1 (MQ=255)                                 gCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      >  2:784155/1‑149 (MQ=255)                                 gCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      >  2:995855/1‑149 (MQ=255)                                    gACCTGTGTCGGCGCCTCCCGTGT‑TTCATTCCCGCC‑AA‑CACCCGCGCATTTACCCACCGCCTTGTGAAAAAAT‑GCCTTC‑GCGGGGGTTGTCGCCCAGTTTTTTTCCCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCaa                                                                                                                   <  2:903562/149‑1 (MQ=255)                                        tgtgTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg                                                                                                                      >  2:396177/1‑142 (MQ=255)                                        tgtgTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑Gaa                                                                                                             >  2:322155/1‑150 (MQ=255)                                        tgggccgggggCTCCCGTGT‑TTCTTTCCCCCC‑AA‑CACCCGCGCTTTTACCCACCCCCATGTGAAAAATT‑CCCTCC‑GCGGCGGTTGTCTGCAAGTTTTTTTCCCGCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑Gaa                                                                                                             <  3:148192/141‑1 (MQ=255)                                             cAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGA‑Gaaaaaaa                                                                                                        >  2:669123/1‑146 (MQ=255)                                               gCGGCTACCGTGT‑TTCATTCCCCCC‑AA‑CCCCCGCGCTTTTCCCCACCGCCATGGAAAAAAAT‑GCCTGC‑GGGGGGGCTGCCCGCCATTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGc                                                                                                        <  3:1105348/148‑1 (MQ=255)                                                 ggCCACCGTAA‑TTTTTTCCCGCC‑AA‑CACCCGCGCTTTTATCAACCGACTTGTGAAAAAAT‑GCCTTC‑GCGGGGGTTGTCTGCCATTTTTTTTGCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑tt                                                                                                     <  2:1397977/148‑1 (MQ=255)                                                  gCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGG‑Gaaaaaaaattt                                                                                                    >  2:1147582/1‑141 (MQ=25)                                                      ccgtgttttCTTTTCCGCC‑AA‑CACCCGCGCATTTGCCAACCGCCATATACAAAATT‑GCCTCC‑GCGGCGACTCCCTCCAACCTTTTTTCCCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTaa                                                                                                  <  2:1067470/140‑1 (MQ=255)                                                      ccGTTA‑TTCTTTCCCGCC‑AA‑CACCCCCCCATTTATCCACCCCATTGGGCAAAAAC‑GCCTCC‑GCGGCGGTTGTCTCCCAGCTTTTTTCCCGCCGCAATAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑t                                                                                                      <  2:1634742/142‑1 (MQ=255)                                                       cGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑Ggaaaaagatatagca                                                                                                >  3:991055/1‑136 (MQ=25)                                                             ttCATTCCCGCC‑AA‑CACCCGCGCTTTTACCCACCGCCATGTGAAAAAAT‑GCCTCC‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCAtg                                                                                        <  2:884911/150‑1 (MQ=255)                                                                       cc‑AA‑CACCCGCGTTTTTACCAACCGCCATGTAAAAAATT‑GCCTGC‑GGGGGGGCTTTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCaa                                                                             <  2:1595156/151‑1 (MQ=255)                                                                               cccGCGCTTTTACCCACCCCAATGTGAAAAAAT‑GCCTCC‑GCGGGGGCTTTCTCCCAGTTTTTTTCCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATc                                                                           <  3:1335253/146‑1 (MQ=255)                                                                                  gcgcTTTTACCAACCGCAATGTG‑AAAAAA‑GCCTTCGGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCg                                                                          <  2:377633/145‑1 (MQ=255)                                                                                   cgtaTTTACCCACCCCCTTGT‑CAAAAATGGCCTTC‑GGGGCGGCTTTCTGCCAGTTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTgg                                                                   <  3:1023012/148‑1 (MQ=255)                                                                                            ccacccccATGTAAAAAAAT‑GCCTCC‑GGGGCGGCTGTCGCCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg                                                           <  3:1157216/144‑1 (MQ=255)                                                                                             cAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAAA‑TTTTAACCCCCCCTGTTTGGTGGGGAACGCTGCTgggtaaaa                                                             >  1:10347/1‑141 (MQ=25)                                                                                                cGCCATGTGAAAAAAT‑GCCTCC‑GCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGaa                                                      <  2:52696/151‑1 (MQ=255)                                                                                                 gCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGG‑GAAAAAACTTTTAAACCCG‑CCTATTTTGTGGGGAACGCTGCTGGttgcaa                                                             >  2:730861/1‑138 (MQ=21)                                                                                                       tgCAAAAAT‑CCCTTG‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa                                                <  2:155345/150‑1 (MQ=255)                                                                                                       gtttaataaggCCCTC‑GCGGCGGCGGTCTGCCAGCTATTTTTCCGCCATAAGAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAAGCGCTGCTGGTAACAATGTGGAAAGGGaa                                                <  2:1379316/141‑1 (MQ=255)                                                                                                          aaaatT‑CCCTCC‑GCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg                                             <  2:922209/150‑1 (MQ=255)                                                                                                          aaaagT‑GCCTTC‑GCGGCGTCTGCCGGCCGGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg                                             <  3:1534811/150‑1 (MQ=255)                                                                                                          aaaaaT‑CCCTTC‑GGGGGGGTTGTCTGCCAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                  <  2:660253/145‑1 (MQ=255)                                                                                                            aatt‑GCCTCC‑GCGGGGGTTGTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                          <  2:506257/148‑1 (MQ=255)                                                                                                            aact‑GCCTCG‑GCGGCGGCTTCCTGCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg                                               <  3:1343315/143‑1 (MQ=255)                                                                                                            aaaaccccTCC‑GGGGCGCCTGTTTGCCATCTTTTTTCCCGCCCAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcg                                           <  2:1071955/145‑1 (MQ=255)                                                                                                             aattcccTTC‑GGGGCGGTTTTCTCCAAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcgg                                          <  3:1253449/146‑1 (MQ=255)                                                                                                              aT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAA‑GAAAAAAA‑TTTTAACCCCCCCTGTTTTGTTGGGAACGCCGCTGGGTACAAAGTGGGAAGGGGAgggggggg                                        >  2:813301/1‑144 (MQ=255)                                                                                                              aT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGGAAAAAAT‑TTTAACCCCCCCAT‑TTTTGTGGGGAACCCTGCTGGGAAAAAAATGGGAAggggaaggggg                                          >  2:1181885/1‑141 (MQ=255)                                                                                                                  ccTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGA‑GAAAAAAC‑ATTAAATCCGCCATGTTTTGTGGGAAATCCTGCCGGTGAAAAAATGGGAAgggggggggggggg                                       >  2:554405/1‑138 (MQ=255)                                                                                                                                      cctttttttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCgcgc                    <  3:847256/142‑1 (MQ=255)                                                                                                                                      ccAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTa                  <  2:927245/151‑1 (MQ=255)                                                                                                                                      ccAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc                        <  3:1442934/145‑1 (MQ=255)                                                                                                                                      ccAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg                     <  2:1199181/148‑1 (MQ=255)                                                                                                                                      ccAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCGGCTCCAGAACCGCGCTa                  <  2:166166/151‑1 (MQ=255)                                                                                                                                      ccAGTTTTTTTCCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg                       <  2:490176/146‑1 (MQ=255)                                                                                                                                      caattttttttcCCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa                          <  3:1144159/136‑1 (MQ=255)                                                                                                                                      caagtttttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgc                      <  3:881320/140‑1 (MQ=255)                                                                                                                                             ttttcCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGAGCGGCGAATCAGCTCCAGAACCGCGCTAATTGa             <  2:457546/149‑1 (MQ=255)                                                                                                                                             ttttcCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcgcg                     <  3:371708/141‑1 (MQ=255)                                                                                                                                               ttccccccgcAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGtt          <  3:1117225/144‑1 (MQ=255)                                                                                                                                                  cccccgaAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTc         <  3:1431513/145‑1 (MQ=255)                                                                                                                                                  cccccgaAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGTTCCAGAACCGCGCTAATTGAGTTc         <  3:1561662/145‑1 (MQ=255)                                                                                                                                                   cgccgcAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGA‑GAAAAAAC‑TTTTAACCCCTCCTGTTGTGTGGGGATCGCTGCTGGTGACAAAGTGGGAAGGGGAGGGGGGGGGATCAGCTccccaa                          >  2:1511820/1‑126 (MQ=255)                                                                                                                                                      cccaaaaaACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCaa  <  3:377166/149‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                                    TGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGA‑TTCATTTCCGCC‑AA‑CAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑GCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGA‑GAAATAGC‑TTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCAA  >  NC_000913/357741‑358035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑