Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,178,239 G→T G166V (GGT→GTT)  nusG → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,2390GT97.7% 350.0 / ‑3.1 88G166V (GGT→GTT) nusGtranscription termination factor
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (43/43);  total (43/45)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAC  >  NC_000913/4178092‑4178386
                                                                                                                                                   |                                                                                                                                                   
agGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCttcgttcg                                                                                                                                                  >  2:1136461/1‑151 (MQ=255)
 ggTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTtcgttcgt                                                                                                                                                 <  3:416793/151‑1 (MQ=255)
  gTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTg                                                                                                                                                <  2:45052/151‑1 (MQ=255)
    tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGc                                                                                                                                               <  2:1140914/150‑1 (MQ=255)
    tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCg                                                                                                                                              >  2:376856/1‑151 (MQ=255)
    tGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCg                                                                                                                                              >  2:634498/1‑151 (MQ=255)
           aaGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCgg                                                                                                                                       >  3:1586451/1‑151 (MQ=255)
             gCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGt                                                                                                                                      >  3:11602/1‑150 (MQ=255)
                gcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTag                                                                                                                                    >  2:1132741/1‑149 (MQ=255)
                 cgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGc                                                                                                                                 >  3:711094/1‑151 (MQ=255)
                  gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCt                                                                                                                                >  3:447298/1‑151 (MQ=255)
                  gTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCt                                                                                                                                >  2:764326/1‑151 (MQ=255)
                    ccGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATGTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTg                                                                                                                               <  2:1625344/150‑1 (MQ=255)
                         aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTc                                                                                                                         <  3:928082/151‑1 (MQ=255)
                               gTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCa                                                                                                                    <  2:1005878/150‑1 (MQ=255)
                               gTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAg                                                                                                                   <  3:1533911/151‑1 (MQ=255)
                                tttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAgg                                                                                                                  >  3:1248146/1‑151 (MQ=255)
                                 ttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAgg                                                                                                                  <  2:1149814/150‑1 (MQ=255)
                                  tGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGt                                                                                                                 <  2:383233/150‑1 (MQ=255)
                                    aaCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGa                                                                                                              <  3:686316/151‑1 (MQ=255)
                                      ccGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaa                                                                                                            <  3:149815/151‑1 (MQ=255)
                                      ccGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaa                                                                                                             >  2:1385469/1‑150 (MQ=255)
                                            gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCt                                                                                                      <  2:965248/151‑1 (MQ=255)
                                            gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCt                                                                                                      >  2:516212/1‑151 (MQ=255)
                                            gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCt                                                                                                      >  3:843969/1‑151 (MQ=255)
                                            gAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCt                                                                                                      >  2:952819/1‑151 (MQ=255)
                                                 ggTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAAcc                                                                                                  <  2:1349798/150‑1 (MQ=255)
                                                  gTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCa                                                                                                <  2:1055560/151‑1 (MQ=255)
                                                    ccGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGc                                                                                              >  3:737986/1‑151 (MQ=255)
                                                     cGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCg                                                                                             >  2:750371/1‑151 (MQ=255)
                                                      gtgtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCg                                                                                             >  2:1063871/1‑150 (MQ=255)
                                                      gtgtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCg                                                                                             >  3:528269/1‑150 (MQ=255)
                                                      gtgtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCg                                                                                             <  3:107401/150‑1 (MQ=255)
                                                       tgtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGAt                                                                                           >  3:723562/1‑151 (MQ=255)
                                                       tgtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGAt                                                                                           >  2:1433464/1‑151 (MQ=255)
                                                          tAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCa                                                                                         <  2:59667/150‑1 (MQ=255)
                                                          tAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCa                                                                                         >  2:968750/1‑150 (MQ=255)
                                                          tAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCa                                                                                         <  2:446180/150‑1 (MQ=255)
                                                           aaTGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                                                                                        >  3:62616/1‑150 (MQ=255)
                                                           aaTGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                                                                                        <  2:1109582/150‑1 (MQ=255)
                                                                     cGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                                             <  3:1572765/151‑1 (MQ=255)
                                                                      gTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                                            >  2:951485/1‑151 (MQ=255)
                                                                      gTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                                            <  2:834866/151‑1 (MQ=255)
                                                                        tCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                                           <  3:879808/150‑1 (MQ=255)
                                                                          gCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                                         >  2:1419548/1‑150 (MQ=255)
                                                                            tGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                                                      >  2:1372573/1‑151 (MQ=255)
                                                                             gACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                                                     >  3:666106/1‑151 (MQ=255)
                                                                                 tCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                                                  <  2:962578/150‑1 (MQ=255)
                                                                                 tCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGc                                                                 >  2:1532837/1‑151 (MQ=255)
                                                                                   aaCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                                               >  2:655304/1‑151 (MQ=255)
                                                                                   aaCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                                               >  3:563938/1‑151 (MQ=255)
                                                                                   aaCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                                               >  3:828002/1‑151 (MQ=255)
                                                                                    aCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                                              <  2:1054735/151‑1 (MQ=255)
                                                                                     cGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                                             <  2:1248265/151‑1 (MQ=255)
                                                                                     cGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                                             <  2:501560/151‑1 (MQ=255)
                                                                                      ggTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                                            <  3:346434/151‑1 (MQ=255)
                                                                                        tgttgtTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                                          >  3:252055/1‑151 (MQ=255)
                                                                                              tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                       >  3:740561/1‑148 (MQ=255)
                                                                                              tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                     <  1:6953/150‑1 (MQ=255)
                                                                                               gaagaaGTGGATTGCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCGTCGGTCGTGCGGCGCCGGTGGAGGTGGGGTGGGGGGGGGTTGGAGGGGGGGGGGGGAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                                                    <  3:790487/150‑1 (MQ=255)
                                                                                               gaagaaGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                                                    <  2:330206/150‑1 (MQ=255)
                                                                                               gaagaaGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                       >  3:1187715/1‑147 (MQ=255)
                                                                                                aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                                                  >  2:1453207/1‑151 (MQ=255)
                                                                                                       ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                                           <  2:441819/151‑1 (MQ=255)
                                                                                                        gATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACa                                          <  3:1162723/151‑1 (MQ=255)
                                                                                                           tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                                       >  3:1246298/1‑151 (MQ=255)
                                                                                                            aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt                                      <  2:136037/151‑1 (MQ=255)
                                                                                                              gagaAATCTCGTCTGGAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg                                    >  3:859643/1‑151 (MQ=255)
                                                                                                              gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg                                    <  3:296781/151‑1 (MQ=255)
                                                                                                               agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc                                   >  2:1543012/1‑151 (MQ=255)
                                                                                                                gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgcg                                  <  3:824168/151‑1 (MQ=255)
                                                                                                                     ctcGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt                             >  2:615423/1‑151 (MQ=255)
                                                                                                                       cGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt                             <  3:1543576/149‑1 (MQ=255)
                                                                                                                       cGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt                              <  3:1079673/148‑1 (MQ=255)
                                                                                                                       cGTCTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg                           <  3:50795/151‑1 (MQ=255)
                                                                                                                          cTGAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttt                        >  3:862216/1‑151 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      >  3:1511732/1‑151 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      >  3:975698/1‑151 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      >  2:1031920/1‑151 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      <  2:1152819/151‑1 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      <  2:126725/151‑1 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtttt                       <  2:376424/150‑1 (MQ=255)
                                                                                                                            gAAAGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttt                        <  3:1284892/149‑1 (MQ=255)
                                                                                                                             aaaGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                      >  3:1488915/1‑150 (MQ=255)
                                                                                                                               aGTGTCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATg                   <  2:723606/151‑1 (MQ=255)
                                                                                                                                gtgtCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATgg                  <  2:1311615/151‑1 (MQ=255)
                                                                                                                                gtgtCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATgg                  >  3:1272202/1‑151 (MQ=255)
                                                                                                                                  gtCTGTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATggg                 <  2:727001/150‑1 (MQ=255)
                                                                                                                                      gTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt              <  3:1197591/149‑1 (MQ=255)
                                                                                                                                      gTTTCTATCTTCGTTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt              <  2:1613697/149‑1 (MQ=255)
                                                                                                                                                tcgttcgtGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAc  >  3:455653/1‑151 (MQ=255)
                                                                                                                                                tcgttcgtGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAc  >  2:645554/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGTTGGTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAC  >  NC_000913/4178092‑4178386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: