Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,806 C→T intergenic (‑202/+33) edd ← / ← zwf 6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,8060CT99.1% 405.2 / ‑3.9 106intergenic (‑202/+33)edd/zwf6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (57/48);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 106 total aligned reads displayed.

CAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCC  >  NC_000913/1934659‑1934953
                                                                                                                                                   |                                                                                                                                                   
caGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGt                                                                                                                                                  <  3:755437/151‑1 (MQ=255)
 agagGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGt                                                                                                                                                  >  2:1119822/1‑150 (MQ=255)
  gagGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTaa                                                                                                                                                <  2:649434/151‑1 (MQ=255)
  gagGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTa                                                                                                                                                 >  2:451239/1‑150 (MQ=255)
  gagGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTa                                                                                                                                                 >  3:1396868/1‑150 (MQ=255)
   agGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTaa                                                                                                                                                >  3:1268409/1‑150 (MQ=255)
   agGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTaa                                                                                                                                                >  2:1345988/1‑150 (MQ=255)
    ggATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTaaaa                                                                                                                                              <  3:110809/151‑1 (MQ=255)
     gATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAAt                                                                                                                                             <  3:440679/151‑1 (MQ=255)
     gATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAAt                                                                                                                                             <  3:210759/151‑1 (MQ=255)
        ttATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATaa                                                                                                                                           <  3:845610/150‑1 (MQ=255)
            gACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATa                                                                                                                                      <  2:1185331/151‑1 (MQ=255)
              cTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATaa                                                                                                                                     >  3:825306/1‑150 (MQ=255)
               tGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATaa                                                                                                                                     >  2:1494242/1‑149 (MQ=255)
                gAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATaaa                                                                                                                                    <  2:620777/149‑1 (MQ=255)
                 aaaCGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAgg                                                                                                                                  >  2:1539520/1‑150 (MQ=255)
                  aaCGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGAt                                                                                                                                <  2:238875/151‑1 (MQ=255)
                   aCGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGAt                                                                                                                                >  2:1244131/1‑150 (MQ=255)
                   aCGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATa                                                                                                                               <  2:536895/151‑1 (MQ=255)
                    cGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATaa                                                                                                                              <  3:783149/151‑1 (MQ=255)
                         gTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAgcg                                                                                                                           <  2:1308381/149‑1 (MQ=255)
                          tAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGAAAAgcgc                                                                                                                          >  3:873343/1‑149 (MQ=255)
                           aaCCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAgcgc                                                                                                                          >  3:1011201/1‑148 (MQ=255)
                                gAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATAtt                                                                                                                   >  2:227838/1‑150 (MQ=255)
                                  gCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACt                                                                                                                <  3:713600/151‑1 (MQ=255)
                                     cATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTc                                                                                                               <  2:1331478/149‑1 (MQ=255)
                                      aTAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCaa                                                                                                             >  2:657034/1‑150 (MQ=255)
                                        aGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAAc                                                                                                           <  3:1563885/150‑1 (MQ=255)
                                        aGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                          <  2:333157/151‑1 (MQ=255)
                                        aGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                          <  3:175967/151‑1 (MQ=255)
                                        aGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACt                                                                                                          <  3:1458970/151‑1 (MQ=255)
                                         gggCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTc                                                                                                         <  2:487673/151‑1 (MQ=255)
                                         gggCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTc                                                                                                         <  2:399137/151‑1 (MQ=255)
                                         gggCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTc                                                                                                         <  3:880969/151‑1 (MQ=255)
                                          ggCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTc                                                                                                         >  3:91832/1‑150 (MQ=255)
                                           gCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCAt                                                                                                       >  2:757574/1‑151 (MQ=255)
                                           gCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCAt                                                                                                       >  2:840602/1‑151 (MQ=255)
                                             aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTc                                                                                                     >  2:249758/1‑151 (MQ=255)
                                             aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTc                                                                                                     >  2:955835/1‑151 (MQ=255)
                                               aaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTcc                                                                                                    >  3:1458738/1‑150 (MQ=255)
                                                aaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTcc                                                                                                    >  2:1549500/1‑149 (MQ=255)
                                                aaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTcc                                                                                                    >  3:1044846/1‑149 (MQ=255)
                                                     ttCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACg                                                                                             >  3:1436455/1‑151 (MQ=255)
                                                        agtcagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGAcc                                                                                          <  2:164742/151‑1 (MQ=255)
                                                          tcagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCAt                                                                                        >  2:680365/1‑151 (MQ=255)
                                                           cagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCAt                                                                                        <  3:368371/150‑1 (MQ=255)
                                                           cagtGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATc                                                                                       >  2:438224/1‑151 (MQ=255)
                                                             gtgtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCAc                                                                                     >  2:1337902/1‑151 (MQ=255)
                                                               gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACgg                                                                                   <  2:1191850/151‑1 (MQ=255)
                                                               gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACgg                                                                                   <  3:1615299/151‑1 (MQ=255)
                                                               gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACgg                                                                                   <  3:1228219/151‑1 (MQ=255)
                                                               gtAATAAAAAAAGCCTCGTGGGCGAATCCGCACGAGGCCTGAAAGCGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACgg                                                                                   >  3:239544/1‑151 (MQ=255)
                                                                 aataaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGt                                                                                 <  3:1416069/151‑1 (MQ=255)
                                                                  ataaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACggg                                                                                  >  3:901229/1‑149 (MQ=255)
                                                                   taaAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTa                                                                                >  2:233038/1‑150 (MQ=255)
                                                                    aaaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAat                                                                              <  3:865264/151‑1 (MQ=255)
                                                                    aaaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAat                                                                              <  3:576289/151‑1 (MQ=255)
                                                                     aaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAatc                                                                             >  2:995712/1‑151 (MQ=255)
                                                                     aaaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAatc                                                                             >  3:76464/1‑151 (MQ=255)
                                                                          aGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGc                                                                        >  1:8804/1‑151 (MQ=255)
                                                                              tCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCAcc                                                                    >  2:132130/1‑151 (MQ=255)
                                                                               cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCAcc                                                                    >  3:808613/1‑150 (MQ=255)
                                                                               cGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCg                                                                   >  3:540452/1‑151 (MQ=255)
                                                                                  gggTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAg                                                                 <  2:541151/150‑1 (MQ=255)
                                                                                  gggTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAg                                                                 <  3:264708/150‑1 (MQ=255)
                                                                                     tGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCa                                                              >  3:1457381/1‑150 (MQ=255)
                                                                                       aaTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACg                                                           <  2:381682/151‑1 (MQ=255)
                                                                                        aTCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACgg                                                          >  3:536081/1‑151 (MQ=255)
                                                                                             cACGAGGCCTGAAAGTGTAAAAATTGATCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTccc                                                     <  2:394588/151‑1 (MQ=255)
                                                                                                gAGGCCTGAAAGTGTAAAAATTGCTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc                                                    >  3:1281037/1‑149 (MQ=255)
                                                                                                 aGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAgg                                                 >  2:1017012/1‑151 (MQ=255)
                                                                                                  ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGt                                                >  3:922570/1‑151 (MQ=255)
                                                                                                  ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGt                                                >  2:666611/1‑151 (MQ=255)
                                                                                                     cTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTcc                                             >  3:560200/1‑151 (MQ=255)
                                                                                                       gAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTcc                                             <  2:825356/149‑1 (MQ=255)
                                                                                                           gtgtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTg                                       <  3:1082557/151‑1 (MQ=255)
                                                                                                            tgtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTg                                       >  3:1331819/1‑150 (MQ=255)
                                                                                                              tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGAt                                     >  3:1401683/1‑150 (MQ=255)
                                                                                                               aaaaaTTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATa                                    <  2:438330/150‑1 (MQ=255)
                                                                                                               aaaaaTTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATAc                                   >  2:772447/1‑151 (MQ=255)
                                                                                                                 aaaTTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACGCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACgg                                 <  3:1186386/151‑1 (MQ=255)
                                                                                                                  aaTTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGt                                <  2:705653/151‑1 (MQ=255)
                                                                                                                   aTTGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGtt                               <  2:56481/151‑1 (MQ=255)
                                                                                                                     tGTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGttt                              <  3:973940/150‑1 (MQ=255)
                                                                                                                      gTTCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTc                             <  3:1145278/150‑1 (MQ=255)
                                                                                                                       ttCTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCgg                           >  3:30054/1‑151 (MQ=255)
                                                                                                                         cTACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcg                         >  3:73132/1‑151 (MQ=255)
                                                                                                                          tACAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGCCCATCACGGGTAATCATCGCCACCGCGGCAACGGGTCCACAGGTTCCGGCCTGACACGGCTTAGgcgc                        >  3:504367/1‑151 (MQ=255)
                                                                                                                           aCAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGgcgc                        >  3:1580951/1‑150 (MQ=255)
                                                                                                                            cAATCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGACAACGTGTCCCCAGGATCCGGCCTGATACGGTTTGGTCGcat                      >  3:235216/1‑151 (MQ=255)
                                                                                                                             aaTCTGCGCAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatc                     >  2:1261016/1‑151 (MQ=255)
                                                                                                                              aTCTGCGCAAGATCATGTTACTGGTAAAATACCCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatc                     >  2:971952/1‑150 (MQ=255)
                                                                                                                                  gcgcAAGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGcatca                    <  3:137174/147‑1 (MQ=255)
                                                                                                                                      aaGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCAt            >  2:346380/1‑151 (MQ=255)
                                                                                                                                      aaGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCAt            >  2:1201937/1‑151 (MQ=255)
                                                                                                                                       aGATCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATc           <  2:236861/151‑1 (MQ=255)
                                                                                                                                         aTCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc         <  3:843413/151‑1 (MQ=255)
                                                                                                                                         aTCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc         >  3:1047410/1‑151 (MQ=255)
                                                                                                                                         aTCATGTTACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGc         <  3:1522829/151‑1 (MQ=255)
                                                                                                                                               ttACTGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGc   >  3:565308/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCC  >  NC_000913/1934659‑1934953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: