Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,712	0	A	C	42.9%	88.2 / 15.1	56	T152P (ACC→CCC)	tus	inhibitor of replication at Ter, DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (25/7);  new base C (0/24);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 8.52e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAACCTTTCGCAAGCAGCGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCAC  >  NC_000913/1684567‑1684862                                                                                                                                                   |                                                                                                                                                        tAACCTTTCGCAAGCAGCGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCtt                                                                                                                                                    >  1:165346/1‑151 (MQ=255)           cAAGCAGCGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTAcc                                                                                                                                          >  1:991376/1‑151 (MQ=255)            aaGCAGCGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCg                                                                                                                                         <  2:707374/151‑1 (MQ=255)              gcagcGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCa                                                                                                                                       <  2:811069/151‑1 (MQ=255)               cagcGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCAc                                                                                                                                      >  1:690691/1‑151 (MQ=255)                 gcGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGc                                                                                                                                    >  1:10035/1‑151 (MQ=255)                      ggTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCAcc                                                                                                                               >  1:736304/1‑151 (MQ=255)                        tcagtcaTATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGt                                                                                                                             >  2:183212/1‑151 (MQ=255)                           gtcaTATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑Ct                                                                                                                         >  2:1006177/1‑151 (MQ=255)                              atatTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGc                                                                                                                       >  1:1023323/1‑150 (MQ=255)                                   cAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGAcc                                                                                                                 <  1:163913/151‑1 (MQ=255)                                      cacaTCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCg                                                                                                              <  1:133224/151‑1 (MQ=255)                                       acaTCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGc                                                                                                             <  1:436481/151‑1 (MQ=255)                                         aTCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCa                                                                                                           <  2:437454/151‑1 (MQ=255)                                               aaaCTCAAGCCCACGTTCGAGCATATCGTCAGGTTGGAATCAGAACCCCCCCCCGCGGCATGTTTTGAAGGGG‑TGCATCGTCTTTTGCCGGGGCTGCCCCCCTTTAATGCTCACCGCACGCTCCCCGTT‑CTGCCCGCCCCCGCCACTTTa                                                                                                      <  1:585622/150‑1 (MQ=255)                                                     aaGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTttg                                                                                               >  2:333907/1‑151 (MQ=255)                                                         ccccGTTGGAGAATTTCGTCAGGTTTAAATCAGACCCCCCCCCCGCGCCACGTTTTAAATGGG‑TGCATCTTCTTTTGCCGGGGCTAACCCCCTTTATTTCTTCCCGCCCGCTCCCCGTT‑CTGCACGACCCCGCCACTTTACGCTttgg                                                                                              <  1:762229/145‑1 (MQ=255)                                                         ccccGTTCGAGCATATCGTCACGGTTAAATCAAACCCCCCCCCCGCGGCACTTTTTGAAGGGG‑TGCTTCGTCTTTTGCCGGGGCTGATCCCCTTTAATGCTCCCCGCACGCCCCCCTTT‑CTGCACGCCCCCGCCACTTTACGCTttggttg                                                                                           <  2:140287/148‑1 (MQ=255)                                                           aCGTTCGAGCATAGCGTCAGGGTTGAATAAGACCCCCCCCCCGCGGCGCTTTTTGAAGGGG‑TGCATCGTCATTTGCGGGGGCTGACCACCCTTAATGCTTCCCGCCCGCTCCCCGTT‑CTGCACGCCCCCGCCACTTTACGCTTTGGTTggg                                                                                         <  2:649676/151‑1 (MQ=255)                                                            cGTTCGAGCATATGGCCCCGGTTAAATCAAACCCCCCCACCGCGGCGCTTTTTGAATGGG‑TGCGTCGTCATTTGCCGGGGCTGATCCCCCTAAATGCTTCCCGCCCGCTCCCCTTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGc                                                                                        <  2:794855/151‑1 (MQ=255)                                                              ttCGAGCATATCGTCAGGGTTGAATCAGACCCCCCCCCCGCGCCCCTTTTTGAAGGGG‑TGCATCGTCTTTTGCGGGGGCTGATCCCCTTTAATGCTTCCCCCACGCCCCCCTTT‑CTCCACGCCCCCGCCACTTTACGCTTTGGTTggg                                                                                         <  2:589085/148‑1 (MQ=255)                                                                cgaccatacgtgccgggTTGACTCAGAACCCCCCCCCGCGGCA‑TTTTTAAATGGGTTGGACCTTCATTTCCCGGGGCTCATCCCCCTTAATGCTTCCCGCCCCCTC‑CCGTTACTCCACGCCCCCGCCACTTTACGCTTTGGTTGGGCtaat                                                                                    <  1:988476/136‑1 (MQ=255)                                                                cGAACATATCGTCACGGTTAAATCAGAACCCCCCCCCGCGGCCCGTTTTGAAGGGG‑TGCATCGTCATTTGCGGGGGCTGATCCCCCTTATTGCTTACCGCCCGCTCACCTTT‑CTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCtaat                                                                                    <  2:388634/151‑1 (MQ=255)                                                                 gAGCATATCGTCACGGTTAAATCAGACCCCCCCCCCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCCCCCTTAATGCTTACCGCACGCTCCCCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGc                                                                                        <  2:359596/146‑1 (MQ=255)                                                                    cATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAAc                                                                                >  2:578491/1‑151 (MQ=255)                                                                              cGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTa                                                                       >  2:205510/1‑150 (MQ=255)                                                                                 ttaattCAGACCCCCCCCCCGCGGCAGGTTTTGAGGGGG‑TGCATCGTCTTTTCCCGGGCCTGATCCCCATTACTGCTAACCGCTCGCTCCCCTTT‑CTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCAt                                                                         <  2:683452/140‑1 (MQ=255)                                                                                    aaacaaaaCCCCCCCCCGCGCCACTTTTGGAAGGGG‑TGCATCGTCTTTGGCCGGGGCTGACCCCCTTTATTGCTTCCCGCACGCCCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAAtt                                                                 <  1:87957/144‑1 (MQ=255)                                                                                    aaTCAGACCCCCCCCCCGCGCCCCTTTTTGAATGGG‑TCCATCGTCTTTTGCCGGGGCTGACCCCCCTTAATGCTTACCGCGCGCTCCCCGTT‑CTGCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGa                                                                    <  1:21423/147‑1 (MQ=255)                                                                                    aaTCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAAttt                                                                >  1:651352/1‑151 (MQ=255)                                                                                    aaTCAGAACCCCCCCCCGCGGCACTTTTTGAAGGGG‑TGCATCGTCATTTGCGGGGGCTGATCCCCCTTAATGCTTCCCGCACGCTCCCCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAAttt                                                                <  2:679744/151‑1 (MQ=255)                                                                                       caaacccccccccCGCGGCATGTTTTGAAGGGG‑TGCTTCGTCATTTGCCGGGGCTGATCCCCTTTATTGCTTCCCGCACGCTCACCTTT‑CTCCCCGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAAtt                                                                 <  1:233025/140‑1 (MQ=255)                                                                                        aGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACAt                                                            >  1:659537/1‑151 (MQ=255)                                                                                        aGAACCCCCCCCCGCGGCATTTTTTGAATGGG‑TGCATCGTTTTTTGCGGGGGCTGATCCCCTTTAATTCTTCCCGCACGCCCCCCGTT‑CTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTAc                                                              <  1:977713/149‑1 (MQ=255)                                                                                          acccccccccCGCGGCGCTTTTTGAGTGGG‑TGCATCGTCATTTGCGGGGGCTGTTCCCCCTTAATGCTTCCCGCCCGCTCCCTTTT‑CTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCg                                                          <  1:1095389/147‑1 (MQ=255)                                                                                           aCTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGt                                                         >  1:608517/1‑151 (MQ=255)                                                                                            ccccccACCGCGGCACGTTTTGAGTGGG‑TCCACCCCCATTTGCGGGGCCTGACCCCCTTTATTGCTTCCCGCCCGCCCCCCGTT‑CTCCCCCACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCg                                                          <  1:738590/147‑1 (MQ=255)                                                                                             tCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAGTAAACATATCATTAAGAATTTACATCGt                                                         >  1:418462/1‑149 (MQ=255)                                                                                                ccccggcgGCCCTTTTTGAGTGGG‑TGCGTCGTCTTTGGCGGGGGCTGACCCCCCTTATTTTTTCCCGCGCGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga                                                    <  1:391557/146‑1 (MQ=255)                                                                                                        gCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTgg                                            >  1:649556/1‑151 (MQ=255)                                                                                                         cACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGc                                           >  2:581199/1‑151 (MQ=255)                                                                                                            gTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGcac                                         >  1:540331/1‑150 (MQ=255)                                                                                                             ttatGAATGGG‑TGCACCGCAATTCGCCGGGGCGAATCCCCCTTATTCCTTCCCCCACGCTCTCCTTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGcac                                         <  2:402104/146‑1 (MQ=255)                                                                                                                  acgggg‑TGCTTCCTCATTTCCCGGGCCTGATCCCCCTTAATGTTTCCCCCCCGCTCCCCTTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGc                                      <  2:503328/144‑1 (MQ=255)                                                                                                                      gg‑TGCATCGTCATTGGCCGGGGCTGATCCCCTTTAATGCTTACCGCACTCTCCCCGTT‑CTGCACCCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGaaaaa                              <  1:678593/151‑1 (MQ=255)                                                                                                                       gtttcttCGTCTTTTGCGGGGGCTGATCCCCTTTAATGCTTCCCGCCCGCCCCCCGTT‑CGGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTgg                                   <  2:275210/140‑1 (MQ=255)                                                                                                                        gcgaatcttaaTTCGCCGGGCCTGTCCCCCTTTATTGCTTCCCCCACGCCCACCTTT‑CTCCACAACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGaaaaaa                             <  1:68337/141‑1 (MQ=255)                                                                                                                              cGTCTTTTGCCGGGGCTAATCCCCCTTAATGCTTCCCGCCCGCTCCCCTTT‑CTCCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAg                            <  1:686385/146‑1 (MQ=255)                                                                                                                                   tttGCCGGGGCTGATCCCCCTTAATGCTTCCCGCACGCCCCCCTTT‑CTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAAt                    <  2:538618/149‑1 (MQ=255)                                                                                                                                    ttGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATcac                 >  1:713266/1‑151 (MQ=255)                                                                                                                                          gggCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGc            >  2:282101/1‑150 (MQ=255)                                                                                                                                             cTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAgtg        >  2:138344/1‑151 (MQ=255)                                                                                                                                              tGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAgtgt       >  1:211341/1‑151 (MQ=255)                                                                                                                                               gATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTc      >  1:465602/1‑151 (MQ=255)                                                                                                                                                aTCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCg     >  1:1127287/1‑151 (MQ=255)                                                                                                                                                  cACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGc    >  2:852835/1‑150 (MQ=255)                                                                                                                                                   aCCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCAc  >  1:165374/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                        TAACCTTTCGCAAGCAGCGTTGGTCAGTCATATTCAGCACATCAATAAACTCAAGACCACGTTCGAGCATATCGTCACGGTTGAATCAGAACTCCCCACCGCGGCACGTTTTGAATGGG‑TGCATCGTCATTTGCCGGGGCTGATCACCCTTAATGCTTACCGCACGCTCACCGTT‑CTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCAC  >  NC_000913/1684567‑1684862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑