Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,206,435 | 0 | C | T | 49.1% | 39.9 / 43.9 | 57 | intergenic (‑179/‑28) | ttdR/ttdA | transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit |
Reads supporting (aligned to +/- strand): ref base C (2/27); new base T (28/0); total (30/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.09e-14 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT > NC_000913/3206286‑3206581 | aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTcc < 2:663475/151‑1 (MQ=255) gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAg < 2:615261/150‑1 (MQ=255) gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa < 2:955769/151‑1 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGa > 2:155218/1‑149 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAc > 1:165963/1‑150 (MQ=255) ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GAATTTTTCAGaaa > 1:711089/1‑147 (MQ=255) ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACaa > 2:462038/1‑150 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAt < 2:1029001/150‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AGTTTTTcttgagaaa > 1:159698/1‑140 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCCCACAAAAACaaa > 1:892606/1‑150 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAAAACaaa > 2:1143129/1‑150 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTgg < 2:247814/150‑1 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCACACAaaaaaaaaaa > 1:1106912/1‑145 (MQ=255) aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGtt < 1:374861/151‑1 (MQ=255) aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGtt < 2:976917/151‑1 (MQ=255) aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAGTTTTTTAAGCAAAAAAAAAAAATGGAggt > 1:365513/1‑149 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTCGACAaaaaaaaaaaatagggtt > 1:1088265/1‑140 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatg < 1:307339/150‑1 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCATACAAAAAAAAAAAATGGAGtttgcttg > 1:712180/1‑141 (MQ=255) gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 1:1134865/150‑1 (MQ=255) aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGAAAAATAAAAAAACTGGTGATGGCATGGTGAGCGaa > 1:517878/1‑149 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAAAGGAGTTGCCATGATGAGCGgaa > 1:994294/1‑148 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:479231/150‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc > 1:350765/1‑150 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa < 1:914317/148‑1 (MQ=255) aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAg < 2:787486/151‑1 (MQ=255) ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:32644/150‑1 (MQ=255) tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa < 2:705642/150‑1 (MQ=255) cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAAAAAAAAAAAAAAATTGAGGTTGCC‑TGATGGGGGAAAGGAAAAAACaaaa > 1:518148/1‑143 (MQ=255) cgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGa < 2:1061253/151‑1 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa < 2:90803/151‑1 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTGTCATGATGAGCGAAAGAAATAAACAAAAAGCAGGGaaaa > 1:63178/1‑146 (MQ=255) tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAACGGAGTTGGCCTGATGAGGGGAAGTAAAAAAAAACAAGCAATGAATaa > 2:1110795/1‑148 (MQ=255) aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa < 1:369717/151‑1 (MQ=255) gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAATATAAAAAATGGAGTTTGCACGATGAGCGAAAGGAATAAAAAACAAGAAGTGAAAAAGt > 1:1061524/1‑146 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGTCATGATGAGCGGAAAGAATAAACAAAAGGCAGGGAATaa > 2:649532/1‑140 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTTGCATGATTAGGGGAAAGAATAAACAAAAAGCaaggaaaaaatt > 1:239370/1‑132 (MQ=255) tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAAAAAAAAAAGGGGTTGGCCTGATGAGGGAAAGAAATAAAAAACAAGCAGTGAATAAATTGaaa > 1:1111945/1‑145 (MQ=255) aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGt < 1:274385/151‑1 (MQ=255) tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg < 2:1035058/150‑1 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATTGAGTTGCCCTGATGAGCGGAAAGAATAAGCAAAAAGCAGTGAATAAGGTGGAAGAGATTTTCGCTAACttt > 2:422868/1‑150 (MQ=255) cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAATGGAGTTTCCCCGATGAGGGGAAGGAATAAAAAACAAGCCAAGGAAAAAATGAAAGAGATTTTCGCTAAAttt > 1:771676/1‑150 (MQ=255) aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGAAAAAAAAAAAAAACTGAGGTGTCCTGGTGGGGGGAAGGAATAAGCAACAAGCAGGGGATAAGGTGGCAGAGAATGTCGCTAACttttaccccccgattt > 2:922843/1‑137 (MQ=255) aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAATATAAAAAATTGAGGTGGCCTGATGAGGGGAAGGAAAAAAAAAAAAGCAGGGAAAAAAATTAAAGAGGATGTCGCCAAATTTTTCGCCAAGAtttttttcc > 1:99868/1‑144 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAACAAAAAATGGAGGTTGCATGATGAGCGGAAGTAATAAACAACAGGCAGGGAATAAATTGACAGAGATTGTCGCTAAATTTTCCGCCATGATTTCTACCCCaaag > 2:63299/1‑148 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTTGAAAAATTAAAAAACAGGAGGTGTCATGATGAGGGGAAAAAAAAAAAAAAAAGGAGGGAATAAAATTACAAAGAATGTTGCTAAATTTTCCGCCATGATTTCTAcccccaagc > 1:687433/1‑143 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGCG‑AATTTTTTCAGACAAAAAAAAAAAACGGGGGTGCCATGATGGGCGAAAAAAAAAAGCAACACGCCGTGAATAAGATGAAAGTGACTATCCGTTACTTTAACCCCAGGAATTTTAcccacacgcc > 2:177926/1‑143 (MQ=255) ttAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAGGAGGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat < 2:234453/150‑1 (MQ=255) gTGGTATTTTGTGGCGGTG‑ATTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCtgatg < 1:451864/148‑1 (MQ=255) tGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg < 1:580649/150‑1 (MQ=255) ggTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt < 1:780434/150‑1 (MQ=255) aGTTTGTGGCGGTG‑AATTTTTTCCAACAAAAACAAAAAATGGGGGTTCCCTGATGAGGGGAAGGAATAAAAAACAAGCAGGGAATAAATTGACAGAGATTGTCGCTAACTTTACCCCCATGATTTTTTCCCGAAAGCCTGATGACgggggg > 1:861181/1‑149 (MQ=255) gTTTGGGGGGGTG‑AATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAACAACAGGTTGTAAATAGGTTGACAGAGATTGTCGCTATTTTGACCGCCATGATTTCTACCCGAATGCCTGATGACgtggt < 1:377943/149‑1 (MQ=255) tttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggt < 2:251670/148‑1 (MQ=255) gtgGCGGTG‑AATTTTTCCAGACAAATAAAAAAACTGGTGTTGCAAAGATGAGAGAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGAAAGAGTTTGCCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa < 1:552581/151‑1 (MQ=255) ggCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaaa < 2:763797/150‑1 (MQ=255) ggCGGTG‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCTTGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGTTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc < 1:462020/151‑1 (MQ=255) aTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc > 1:384924/1‑149 (MQ=255) ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt > 2:997037/1‑151 (MQ=255) | AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT > NC_000913/3206286‑3206581 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |