Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,457,105	0	A	G	43.5%	79.4 / 22.9	62	I441V (ATC→GTC)	mpl	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base A (3/32);  new base G (27/0);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.45e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTC  >  NC_000913/4456955‑4457255                                                                                                                                                        |                                                                                                                                                       cATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGa                                                                                                                                                        <  2:275990/151‑1 (MQ=255)   tatTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGG‑GGGGTc                                                                                                                                                      >  2:617034/1‑151 (MQ=255)    atTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATcc                                                                                                                                                     <  1:959528/151‑1 (MQ=255)     ttCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGG‑GGGGTcc                                                                                                                                                     >  2:381384/1‑150 (MQ=255)         gtggcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAg                                                                                                                                                <  1:845776/151‑1 (MQ=255)         gtggcaggtggcagAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAg                                                                                                                                                <  1:68162/151‑1 (MQ=255)                gtggcagaagtggcagaaGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAActgc                                                                                                                                         <  2:810440/151‑1 (MQ=255)                   gcagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTgg                                                                                                                                      <  2:169295/151‑1 (MQ=255)                    cagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGTTCCTCAGGAACTGATgg                                                                                                                                      >  1:500113/1‑150 (MQ=255)                    cagaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGGTTTGGT‑GGGGTCCATCAGAAAATGCTgg                                                                                                                                      >  1:534753/1‑150 (MQ=255)                     agaagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGTCCATCAGAAAATGCTgg                                                                                                                                      >  2:375352/1‑149 (MQ=255)                       aagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATgg                                                                                                                                  <  2:769524/151‑1 (MQ=255)                       aagtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGGTTTGGG‑GGGGACCATCAGAAAATGCTGGGTgg                                                                                                                                  >  1:505465/1‑151 (MQ=255)                        agtggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATggg                                                                                                                                 <  1:284104/151‑1 (MQ=255)                           ggcagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCt                                                                                                                               <  2:756469/150‑1 (MQ=255)                             cagaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGG‑GGGGTCCATCAGAAAATGCTGGGTGGGCTgg                                                                                                                             >  2:731267/1‑150 (MQ=255)                              agaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGGTTTTGT‑GGGGGCCATCAAAAAATGCTGGGTGGGGGGGCg                                                                                                                           >  1:1075483/1‑151 (MQ=255)                               gaagCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCga                                                                                                                          <  2:1064537/151‑1 (MQ=255)                                 agCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCCGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGGCCATATTCTGGTGGTGAGCAACGGCGGCTTTTGG‑GGGGTTCATGCCAAAGTGCTGGGGGGGCgggag                                                                                                                           >  1:109086/1‑146 (MQ=255)                                      gCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGG‑GGGGTCCCTCCGAAAATGGTTGGTGGGGTGGCGGAGAAgg                                                                                                                    >  1:786595/1‑150 (MQ=255)                                       cGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCg                                                                                                                  <  2:440841/151‑1 (MQ=255)                                        gTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCgg                                                                                                                 <  2:741419/151‑1 (MQ=255)                                           cAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGT‑GGGGGCCATCAGAAAATGCTGGGTGGGCTGGCGAAGAAAGCgg                                                                                                                 >  2:10219/1‑148 (MQ=255)                                               cTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGT‑GGGGTCCCTCAGAAAAAGCTGGGTGGGGTGGCggagaaagggggaggc                                                                                                            >  1:312115/1‑139 (MQ=255)                                                tGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGTGGGGATCC‑TCAGAAACAGCTGGAAGGGGTGGGgaaaaaagggg                                                                                                                 >  1:269303/1‑138 (MQ=255)                                                 gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgcg                                                                                                         <  1:925283/150‑1 (MQ=255)                                                 gCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCcgc                                                                                                          <  1:821177/149‑1 (MQ=255)                                                    cacTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGt                                                                                                     <  1:102034/151‑1 (MQ=255)                                                        ggAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTaa                                                                                                   <  2:745440/149‑1 (MQ=255)                                                          aGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCg                                                                                               <  2:141122/151‑1 (MQ=255)                                                              gCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCt                                                                                           <  2:737376/151‑1 (MQ=255)                                                                 aTGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTAGTGATGAGCAACGGCGGTTTTGGT‑GGGGTCCATCAGAAAATGCTGGATGGGGTGGCGAAGAAGGGGGAAGCCGCGCCGTAATTCGGCCtt                                                                                          >  2:192335/1‑149 (MQ=255)                                                                    tgGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGG‑GGGGTCCATCAGAAAATGCTGGATGGGCTGGCGGAGAAGGCGGGAGCCGCGCAGTAATTCGGCCCTAGcc                                                                                      >  2:1044105/1‑150 (MQ=255)                                                                       aTACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGat                                                                                  <  1:483267/151‑1 (MQ=255)                                                                            cTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGG‑GGGGTCCCTCCGAAAATGCTGGATGGGGCGGCGGAGAAGGGGGGAGCCCCGCAGGAAATTGGGCTTAGcccg                                                                                    >  1:112660/1‑142 (MQ=255)                                                                              ggCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGt                                                                             <  1:314066/149‑1 (MQ=255)                                                                                    tatGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGG‑GGGGTCCATCAGAAACTGGTGGATGGGCTGGGGGAGAAAGCGGGAGCCGCGCCGTAATTTGGCCTTAGCCAGATATAGTAt                                                                           >  2:703029/1‑145 (MQ=255)                                                                                     atGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAg                                                                     <  1:368497/150‑1 (MQ=255)                                                                                         tggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGTCCCTCAGAAACTGCTGGATGGGCTGGGGAAGAAGGCGGGAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCgg                                                                >  2:756808/1‑151 (MQ=255)                                                                                          ggtgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGG‑GGGGTCCATCAGAAAATGCTGGGTGGGCTGGCGGAGAAAGCGGAAGCCGCGCAGTAATTTGGCCTTAGCCAGATATAGTATGCGTAGGCCgg                                                                >  2:630549/1‑150 (MQ=255)                                                                                            tgAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGCTTTGGT‑GGGGTCCATCAGAAACTGCTGGGTGGGGTGGCGGAGAAGGGGGAAGCCGCGCCGTAAATCGGCCCTAGCCAGATATAGTATGCGTAGGCCgg                                                                >  1:175384/1‑148 (MQ=255)                                                                                                aaCCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGc                                                         <  2:149613/151‑1 (MQ=255)                                                                                                       cAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc                                                   <  1:729729/150‑1 (MQ=255)                                                                                                        aGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAc                                                   <  1:245987/149‑1 (MQ=255)                                                                                                         gCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCAcgcc                                                <  1:701506/151‑1 (MQ=255)                                                                                                           cTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTTGG‑GGGGTCCATCAGAAAATTGTGGGGGGGGTGGGGGAGAAGGGGGGAGCCGCGCAGGAATTCGGCCTTAGCCAGGTATAGTAAGCGGAGGCCGGGTAAGGCGTTCAcgcc                                                >  1:352694/1‑149 (MQ=255)                                                                                                              gCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGGCCCTCAAAAACTGCTGGATGGGCGGGGGGAGAAGGGGGGAGCCGCGCAGTAATTTGGGCTTTGCCAGATATAGGATTCGTAGGCCGGGTAAGGGGGTTACGCCGCATc                                           >  1:352298/1‑151 (MQ=255)                                                                                                              gCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                           <  1:854447/151‑1 (MQ=255)                                                                                                               cGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                          <  1:536025/151‑1 (MQ=255)                                                                                                                 aCCATATTCTGGTGATGAGCAACGGCGGTTTTTGG‑GGGGTCCCTCAGAAACTGCTGGGTGGGCTGGCGGAGAAGGCGGGAGGCGCGCAGTAATTCGGCCTTTGCCAGATATAGTATGCGTAGGCCGGGTAAAGCGGTCACGCCGCATCCgg                                        >  1:213099/1‑151 (MQ=255)                                                                                                                  ccATATTCTGGTGATGAGCAACGGCGGTTTTTGT‑GGGGTCCATCAGAAACTGCTGGATGGGGTGGCGAAGAAGGCGGAAGACGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGGTAAGGCGGTCACGCCGCATCCgg                                        >  2:805092/1‑150 (MQ=255)                                                                                                                  ccATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGTCCCTCAGCAACTTCTGGAGGGGCCGGCGGAGAAGGCGGGAGCCGCGCCGCAATTCGGGCTTCGCCAGATATAGTATGCGCAGGCCGGGTAAGGCCTTCACGCCCCAccccg                                        >  1:860620/1‑148 (MQ=255)                                                                                                                  ccATATTCTGGTGATGAGCAACGGCGGGTTTTGG‑GGGGTCCCTCAGAAACTGCTGGATGGGGTGGGGGAGAAAGCGGGAGCCGCGCAGGAATTCGGCCCTAGCCAGAAATAGGATGCGGAGGCCGGGTAAGGCGGTCACGCCGCCTcccg                                        >  1:923191/1‑148 (MQ=255)                                                                                                                    atatTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAt                                     <  2:835197/151‑1 (MQ=255)                                                                                                                      atTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGTCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCCGATATAGTATGCGTAGGCCGGGTAAGGCGTTCACGCCGCATCCGGCAttt                                   >  2:1114516/1‑151 (MQ=255)                                                                                                                      atTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGGACCATCAGAAACAGCTGGATGGGGTGGCGGAAAAGGCGGAAACCGCGCAGTAATTTGGCCTTAGCCAGATATAGTATGCGGAGGCCGGGGAAGGCGGTCACGCCCCATCCGGCCttt                                   >  2:563010/1‑151 (MQ=255)                                                                                                                        tCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTTGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTg                                  <  2:652548/150‑1 (MQ=255)                                                                                                                        tCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTg                                  <  1:617012/150‑1 (MQ=255)                                                                                                                                           ggTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGa               <  1:558230/150‑1 (MQ=255)                                                                                                                                           ggTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGAc              >  1:776135/1‑151 (MQ=255)                                                                                                                                             ttttGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGc            >  2:685423/1‑151 (MQ=255)                                                                                                                                                 ggt‑ggGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGTGACGCTTGa        <  1:655507/151‑1 (MQ=255)                                                                                                                                                      ggATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCg    <  2:618404/151‑1 (MQ=255)                                                                                                                                                        aTCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTc  >  1:513015/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                       CATATTCCGTGGCAGGTGGCAGAAGTGGCAGAAGCCTGCGTTCAGCCTGCACACTGGAGTGGCGATGTGGATACGCTGGCAGATATGGTGGTGAAAACCGCTCAGCCTGGCGACCATATTCTGGTGATGAGCAACGGCGGTTTTGGT‑GGGATCCATCAGAAACTGCTGGATGGGCTGGCGAAGAAGGCGGAAGCCGCGCAGTAATTCGGCCTTAGCCAGATATAGTATGCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTGAGCATAGTGCCTGATGCGACGCTTGATGCGTC  >  NC_000913/4456955‑4457255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑