Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,257,602	A→T	N163I (AAT→ATT)	lexA →	transcriptional repressor of SOS regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,257,602	0	A	T	100.0%	306.9 / NA	82	N163I (AAT→ATT)	lexA	transcriptional repressor of SOS regulon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (43/39);  total (43/39)

GCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGC  >  NC_000913/4257455‑4257748                                                                                                                                                    |                                                                                                                                                   gcgTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTa                                                                                                                                                  >  2:597800/1‑150 (MQ=255)  cgTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTaaa                                                                                                                                                <  2:1097914/151‑1 (MQ=255)  cgTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTaaa                                                                                                                                                <  1:510691/151‑1 (MQ=255)   gTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTaa                                                                                                                                                 >  2:661894/1‑149 (MQ=255)    tCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGt                                                                                                                                              >  2:11293/1‑151 (MQ=255)     cAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGt                                                                                                                                              <  2:409160/150‑1 (MQ=255)     cAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTc                                                                                                                                             >  1:868884/1‑151 (MQ=255)         gggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAAc                                                                                                                                         >  2:1027307/1‑151 (MQ=255)         gggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAAc                                                                                                                                         <  1:238628/151‑1 (MQ=255)              gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGtt                                                                                                                                     <  2:1124891/150‑1 (MQ=255)              gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGtt                                                                                                                                     <  1:608587/150‑1 (MQ=255)              gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTg                                                                                                                                    <  2:21235/151‑1 (MQ=255)                cGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGcc                                                                                                                                  >  2:785363/1‑151 (MQ=255)                 gATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCa                                                                                                                                 <  1:826253/151‑1 (MQ=255)                      aaGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGaaa                                                                                                                             >  1:1098824/1‑150 (MQ=255)                       aGATATCGGCATTATTGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACTTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAATTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCTATAAAAt                                                                                                                           <  1:350898/151‑1 (MQ=255)                         atatCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAg                                                                                                                         <  2:140541/151‑1 (MQ=255)                           atCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCg                                                                                                                       >  2:970531/1‑151 (MQ=255)                              ggCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGt                                                                                                                    <  1:754201/151‑1 (MQ=255)                               gCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGtt                                                                                                                   <  1:296893/151‑1 (MQ=255)                               gCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGtt                                                                                                                   >  2:992370/1‑151 (MQ=255)                                cATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                  >  2:882738/1‑151 (MQ=255)                                 aTTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTAATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                  >  2:1155614/1‑150 (MQ=255)                                     tggatggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAAcc                                                                                                             >  1:1098855/1‑151 (MQ=255)                                     tggatggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAAcc                                                                                                             >  1:62303/1‑151 (MQ=255)                                        atggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAAt                                                                                                          >  1:1008354/1‑151 (MQ=255)                                         tggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAAt                                                                                                          <  1:147816/150‑1 (MQ=255)                                             gACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTc                                                                                                      <  2:998092/150‑1 (MQ=255)                                                ttGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg                                                                                                  >  2:829902/1‑151 (MQ=255)                                                    tGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCt                                                                                              >  2:781968/1‑151 (MQ=255)                                                     ggCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAcc                                                                                               <  2:262243/149‑1 (MQ=255)                                                         gTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc                                                                                         <  2:659083/151‑1 (MQ=255)                                                          tGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTc                                                                                         >  2:1088281/1‑150 (MQ=255)                                                           gCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTca                                                                                        <  2:663726/150‑1 (MQ=255)                                                                     cAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc                                                                              >  2:606266/1‑150 (MQ=255)                                                                     cAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTc                                                                              >  1:385359/1‑150 (MQ=255)                                                                        gATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAcc                                                                           >  1:548672/1‑150 (MQ=255)                                                                         aTGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAt                                                                         >  2:661829/1‑151 (MQ=255)                                                                         aTGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAt                                                                         <  1:509541/151‑1 (MQ=255)                                                                          tGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAtt                                                                        >  1:1149936/1‑151 (MQ=255)                                                                          tGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAtt                                                                        >  2:289392/1‑151 (MQ=255)                                                                             aCGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGa                                                                      >  2:572050/1‑150 (MQ=255)                                                                              cGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGa                                                                      >  1:1124176/1‑149 (MQ=255)                                                                                 aaCGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAggg                                                                  <  1:517991/150‑1 (MQ=255)                                                                                      tCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCg                                                            >  1:278465/1‑151 (MQ=255)                                                                                          gTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTg                                                        <  1:65174/151‑1 (MQ=255)                                                                                             gTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTgggg                                                     >  2:350551/1‑151 (MQ=255)                                                                                             gTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTgggg                                                     <  1:274217/151‑1 (MQ=255)                                                                                                gTCGCACGTATTGATGACGAAGTT‑CCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTAt                                                 <  2:994203/151‑1 (MQ=255)                                                                                                  cGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTAtt                                                <  1:1117366/151‑1 (MQ=255)                                                                                                   gCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTAtt                                                >  2:122873/1‑150 (MQ=255)                                                                                                     aCGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGc                                             >  1:293124/1‑151 (MQ=255)                                                                                                        tATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCaa                                           >  2:58589/1‑150 (MQ=255)                                                                                                         aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAAc                                          >  2:735104/1‑150 (MQ=255)                                                                                                           tgatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACgg                                        >  1:37281/1‑150 (MQ=255)                                                                                                            gatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCg                                      <  1:492537/151‑1 (MQ=255)                                                                                                            gatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCg                                      >  2:765891/1‑151 (MQ=255)                                                                                                                 cGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTgg                                 >  2:883844/1‑151 (MQ=255)                                                                                                                   taGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGGTGGGGGTATTCGCCAAGGAGACAgggt                               >  1:1144900/2‑149 (MQ=255)                                                                                                                   aaGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTGATTCGCAACGGCGACTg                                  >  1:824764/1‑148 (MQ=255)                                                                                                                    aGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTg                                  >  1:672760/1‑147 (MQ=255)                                                                                                                     gTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGt                             <  1:992335/151‑1 (MQ=255)                                                                                                                       tACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGt                             >  2:536434/1‑149 (MQ=255)                                                                                                                        aCCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTaa                           <  1:393892/150‑1 (MQ=255)                                                                                                                        aCCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAAc                          <  1:311650/151‑1 (MQ=255)                                                                                                                          cGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACat                        <  1:618734/151‑1 (MQ=255)                                                                                                                            ttAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACatat                      >  2:101479/1‑151 (MQ=255)                                                                                                                             tAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACatat                      <  1:705362/150‑1 (MQ=255)                                                                                                                              aaGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATAtct                    <  2:689373/151‑1 (MQ=255)                                                                                                                               aGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATAtctc                   <  2:654972/151‑1 (MQ=255)                                                                                                                                gcgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATctct                  <  2:293518/151‑1 (MQ=255)                                                                                                                                 cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTTGCAACGGCGACCGGCTGTAAAattattct                  >  1:823837/1‑144 (MQ=255)                                                                                                                                 cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTg                 <  1:179408/151‑1 (MQ=255)                                                                                                                                 cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTg                 <  1:970497/151‑1 (MQ=255)                                                                                                                                 cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTGGCTGTTAAATATctct                  >  2:877618/1‑150 (MQ=255)                                                                                                                                  gcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTga                <  2:877271/151‑1 (MQ=255)                                                                                                                                        aaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAAGAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACcgc          <  2:206346/151‑1 (MQ=255)                                                                                                                                           aaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGaa       <  1:575132/151‑2 (MQ=255)                                                                                                                                            aCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGAATGGGTGGAACATATTTCTGAGGACGCGa        >  2:72827/1‑149 (MQ=255)                                                                                                                                              aGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTTGGGGTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgcc    >  1:953028/1‑151 (MQ=255)                                                                                                                                               gggCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgccg   <  1:215834/151‑1 (MQ=255)                                                                                                                                                ggCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgccgc  <  1:816325/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCGCACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGC  >  NC_000913/4257455‑4257748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑