Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,453	T→G	E212A (GAA→GCA)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,453	0	T	G	100.0%	236.0 / NA	62	E212A (GAA→GCA)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/31);  total (31/31)

TGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAAC  >  NC_000913/3400306‑3400598                                                                                                                                                    |                                                                                                                                                  tGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCAc                                                                                                                                                >  1:155120/1‑151 (MQ=255)  gCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCAc                                                                                                                                                <  2:388911/150‑1 (MQ=255)     gTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGAt                                                                                                                                            <  1:643029/151‑1 (MQ=255)     gTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGAt                                                                                                                                            <  2:224126/151‑1 (MQ=255)       gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATc                                                                                                                                           <  2:1059999/150‑1 (MQ=255)       gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATc                                                                                                                                           >  2:7058/1‑150 (MQ=255)       gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCa                                                                                                                                          <  1:559341/151‑1 (MQ=255)       gCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCa                                                                                                                                          <  2:562954/151‑1 (MQ=255)         ttCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATc                                                                                                                                           >  1:1041787/1‑148 (MQ=255)            gAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAgaga                                                                                                                                      >  2:328065/1‑150 (MQ=255)            gAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAgaga                                                                                                                                      >  2:102638/1‑150 (MQ=255)              ggATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAAcc                                                                                                                                   >  2:1084486/1‑151 (MQ=255)                    cATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAAtt                                                                                                                             >  1:118111/1‑151 (MQ=255)                    cATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAAtt                                                                                                                             <  2:322397/151‑1 (MQ=255)                    cATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAAt                                                                                                                              <  1:955450/150‑1 (MQ=255)                       tGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCATAGAACCGTAATTac                                                                                                                           <  1:85794/150‑1 (MQ=255)                        ggAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTacg                                                                                                                          <  2:698269/150‑1 (MQ=255)                        ggAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTacg                                                                                                                          <  2:14603/150‑1 (MQ=255)                          aGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTacgacg                                                                                                                       >  1:433335/1‑151 (MQ=255)                           gTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGc                                                                                                                      >  1:395730/1‑151 (MQ=255)                             tCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGca                                                                                                                     <  2:880469/150‑1 (MQ=255)                                    aaaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtg                                                                                                             <  2:500536/151‑1 (MQ=255)                                    aaaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtg                                                                                                             >  1:154726/1‑151 (MQ=255)                                     aaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtg                                                                                                             >  1:766283/1‑150 (MQ=255)                                     aaaCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTAGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtg                                                                                                             >  2:187583/1‑150 (MQ=255)                                       aCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtgatg                                                                                                          >  1:1024045/1‑151 (MQ=255)                                       aCCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTtgatg                                                                                                          >  2:42241/1‑151 (MQ=255)                                         cgcgTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTgatgat                                                                                                        >  2:882831/1‑151 (MQ=255)                                                 acacCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTtcgt                                                                                                <  1:309117/151‑1 (MQ=255)                                                  cacCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTtcgtc                                                                                               <  1:364628/151‑1 (MQ=255)                                                        cTGCCAGGTTACGGCCACGAACTTCGATTTCACGGCCTTCATCGCCCGGATAAGCCGAACCGATTTCGCGCTTGATACGTTCTGCGGCGCCTGCACCGATCAGAGAACCGAAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACg                                                                                         >  1:679200/1‑151 (MQ=255)                                                        cTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACg                                                                                         >  2:602648/1‑151 (MQ=255)                                                            cAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTca                                                                                     <  2:1129923/151‑1 (MQ=255)                                                                       cACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATgcg                                                                          >  1:499729/1‑151 (MQ=255)                                                                         cGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGca                                                                        <  2:8587/151‑1 (MQ=255)                                                                                cGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACagaag                                                                  <  1:433151/150‑1 (MQ=255)                                                                                cGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACaga                                                                    >  1:297629/1‑148 (MQ=255)                                                                                  aTTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAgagg                                                               >  1:274696/1‑151 (MQ=255)                                                                                    ttCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAgagg                                                               >  1:961391/1‑149 (MQ=255)                                                                                      cACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGt                                                            <  2:120625/150‑1 (MQ=255)                                                                                              tCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACca                                                      <  1:847495/148‑1 (MQ=255)                                                                                               cATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCacac                                                   <  1:678225/150‑1 (MQ=255)                                                                                               cATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACAcc                                                  >  2:348170/1‑151 (MQ=255)                                                                                                aTCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCg                                                 <  2:298503/151‑1 (MQ=255)                                                                                                    cccGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTc                                              >  1:610667/1‑150 (MQ=255)                                                                                                    cccGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCa                                             <  2:943814/151‑1 (MQ=255)                                                                                                              ccGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAAc                                   >  1:85111/1‑151 (MQ=255)                                                                                                                     gATTTCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACt                            <  2:676408/151‑1 (MQ=255)                                                                                                                        ttCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc                          >  2:1005683/1‑150 (MQ=255)                                                                                                                        ttCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc                          <  1:88655/150‑1 (MQ=255)                                                                                                                        ttCGTGCTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc                          <  1:813727/150‑1 (MQ=255)                                                                                                                              cTTGATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGt                    >  2:1131138/1‑150 (MQ=255)                                                                                                                                 gATACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacca                <  1:710618/151‑1 (MQ=255)                                                                                                                                  aTACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCAACACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccac               >  1:203921/1‑151 (MQ=255)                                                                                                                                   tACGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccacc              >  1:1144910/1‑151 (MQ=255)                                                                                                                                    aCGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccacca             <  2:1101108/151‑1 (MQ=255)                                                                                                                                     cGTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccaccac            <  1:1007606/151‑1 (MQ=255)                                                                                                                                      gTTCTGCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTaccaccacc           >  2:584648/1‑151 (MQ=255)                                                                                                                                           gCGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGat        <  2:819794/149‑1 (MQ=255)                                                                                                                                            cGGTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATc     >  1:487238/1‑151 (MQ=255)                                                                                                                                              gTGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCGTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGat        <  2:826951/146‑1 (MQ=255)                                                                                                                                               tGGCTGCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAac  >  1:997871/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  TGCAGTGCTTCGAGGATTTCATTGGAGTTCAGGGTAAAACCGCGTGGAACACCTTCTGCCAGGTTACGGCCACGAACTTCGATTTCACGGACTTCATCGCCCGGATAAGCCGAACCGATTTCGTGCTTGATACGTTCTGCGGTGGCTTCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATCAAC  >  NC_000913/3400306‑3400598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑