Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,478,3240TG41.7% 90.5 / 25.4 61G33G (GGT→GGGynbBputative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base T (4/31);  new base G (25/0);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 4.57e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT  >  NC_000913/1478185‑1478473
                                                                                                                                             |                                                                                                                                                        
aaTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACAtt                                                                                                                                               <  1:565137/151‑1 (MQ=255)
 aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAGGGGGGGGGGTTAAAtt                                                                                                                                               >  1:1062750/1‑150 (MQ=255)
       gTCTGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGGTGATAAACCGTTTTCTGCTATTGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTAtt                                                                                                                                        <  1:774257/151‑1 (MQ=255)
        tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCTGGGAGAAAAGGGGGGGAGGTTaa                                                                                                                                                  >  1:707602/1‑139 (MQ=255)
               gtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCCGAGAGGAAAAGGGGGGTGGGTACAATACGCATTCGTAcc                                                                                                                                 >  2:754496/1‑149 (MQ=255)
                  cttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTCGGGGGGAAAAGGGGGTGGGGGTTTATTTTGTATTCGTACCt                                                                                                                                >  1:815773/1‑147 (MQ=255)
                  cttcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGAGAGAAAAGGGGGGGGGGGTATATTTCGTATTTGTACCtgg                                                                                                                             >  1:618653/1‑149 (MQ=255)
                    tcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCtggtgg                                                                                                                          <  1:1001045/150‑1 (MQ=255)
                      ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAAGGGGGGGGGGTACATTTCGTATTCGTACCTGggggggg                                                                                                                       >  2:396103/1‑150 (MQ=255)
                          gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCg                                                                                                                   <  1:720602/151‑1 (MQ=255)
                           aGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACCGGGAGAAAAGGGGGGGGGGGTACATTACGTATTTTTTACTGGTGGGGGATCg                                                                                                                   >  1:830994/1‑150 (MQ=255)
                             cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCCGGGAGAAAAAGGGGGGAGGGTACATTAAGTATTTGTAACTGGTGGGGGATCGTc                                                                                                                >  2:738782/1‑150 (MQ=255)
                                    atatAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGttt                                                                                                        <  1:528320/151‑1 (MQ=255)
                                         aTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCaa                                                                                                   <  1:724376/151‑1 (MQ=255)
                                              ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATgg                                                                                                <  1:96633/149‑1 (MQ=255)
                                                  aaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGAGAGAAAAGGGGGGGGGGGTAAATTTCGTATTTGTTCCCGGGGGGGGAGCGTCCTTTGTTTTTTTATGGttgttt                                                                                          >  1:816970/1‑146 (MQ=255)
                                                  aaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGAttt                                                                                          <  1:1184287/151‑1 (MQ=255)
                                                   aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGGGGAAAGGGGGGGGGGGTACATTACGTATTTGTACCTGGGGGGGGATCGGCATTTGttttttcattggggttt                                                                                          >  2:6296/1‑138 (MQ=255)
                                                   aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTc                                                                                         <  1:1018463/151‑1 (MQ=255)
                                                   aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGGGGGGGTACATTAAGTATTCGTACCCGGGGGGGGGACGTCATTTGtttttttattggggtttt                                                                                         >  1:796756/1‑138 (MQ=255)
                                                     tctcTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACCGGGAGGAAAAGGGGGGGGGTTTAAATTCGTATTTTTAACTTGTGGGGGATTCTCTTTTTTTTTTTTATTGTGGttt                                                                                          >  2:234551/1‑148 (MQ=255)
                                                     tctcTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAACGGGGAGGAAAGGGGGGGGGGTTAGATTACGCATTTGTTCCCAGTGGGGCGTCGGCATTTTTTTTTTCATTGTGtttt                                                                                          >  2:462279/1‑148 (MQ=255)
                                                        ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATTGCGGTTACCGGAGAGAAAAGGGGGTGTGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCa                                                                                        <  1:529342/147‑1 (MQ=255)
                                                        ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACGGGGGGAAAAATGGGGGAGGGTACATTTACTATTCACACCCTGGGGGGGATCTTCCTtttttttttttttgtgtatt                                                                                          >  1:984096/1‑133 (MQ=255)
                                                          ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGAGAAAAAGGGGGGGGGGTACATTTCGTATTTGTAACTGGTGGGGGATCGTCATTTTTTTTTTTATTGGGATTTTAGGGcc                                                                                   >  2:491026/1‑150 (MQ=255)
                                                          ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGt                                                                                     <  2:737108/148‑1 (MQ=255)
                                                          ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTc                                                                                    <  1:1162158/149‑1 (MQ=255)
                                                          ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTc                                                                                    <  2:639105/149‑1 (MQ=255)
                                                            cAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAAGGGGGGGGGGTACATTACGTATTCGTACCTGGTGGGGGGTCGTCATTTTTTTTTTTATTGGGATTTTAGGGCCAc                                                                                 >  2:452911/1‑150 (MQ=255)
                                                             aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTCCCGGAGGGAAAAGGGGGGGGGGGTAAATTAACGCTTTATACCCCGTGGGGGATCGTCCTTTGTTTTTTAATTGGGAttttcgggccccg                                                                                >  2:1179811/1‑140 (MQ=255)
                                                                     tCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGAGGAAAGGGGGGGGGGGTACATTACGCATTTGTACCTGGGGGGGCATCGTCATTTGTTTTTTAATGGTGGTTTCAGGGGCACGCTgg                                                                            >  2:718333/1‑146 (MQ=255)
                                                                     tCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGGGGGGTTACATTTAGTATTTGTACCTGGGGGGGGGTCGGCATTTTTTTTTTTATAGGGATTTTTGGTCCACGc                                                                               >  1:892179/1‑143 (MQ=255)
                                                                      cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTATCGGTGGGAAAAGGGGGGGAGGGGACATTAAGTATTTGTACCTGGGGGGGGGTCGTCATTTTTTTTTTTATGGTGATTTCAGGGTCCCGCTGGGtgacg                                                                      >  1:948084/1‑151 (MQ=255)
                                                                      cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGGAAAGGGGGGGGGGGTACATTACGTATTTGTACCTGGTGGGGGATCGTCATTTTTTTTTTAATTGGGGTTTTAGGGCCACGCTGGGtgacg                                                                      >  2:825984/1‑151 (MQ=255)
                                                                      cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGATAACGGAGAGAAAAGGGGGCGGGGGTACATTTCCGGTTCTTCTCTCGGGGGGGCACGGCTCGTTTTTTTTCATGGTGATTTTAGGGCCCCGCTgggagggg                                                                      >  1:876538/1‑145 (MQ=255)
                                                                            gttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCCGGGGAAAAAAGGGGGGGAGGGTTCCTTTCTTATTTGTACCTGGGGGGGGATCGTCATTTTTTTTTTTATGGTGGTTTTAGGGCCACGCTGGGtgacgttgtc                                                                 >  1:267411/1‑148 (MQ=255)
                                                                                 ttttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTTTGGGGAAAAAAAGGGGTGAAGGTAAAATATGTAAACGTGCCTCGTGGGGGATCCTCGTTTGTTTTTTTTTTGTTATTTCAAGGCCTCGCTGGATGTGGTTTCCGttttt                                                           >  1:152060/1‑151 (MQ=255)
                                                                                  tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGttttt                                                           <  2:235388/150‑1 (MQ=255)
                                                                                   ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGtttttt                                                          <  1:776066/150‑1 (MQ=255)
                                                                                    tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGGGAGAAAAAGGGGGGGGGGTACATTACGGATTTGTACCCGGGGGGGGATCGTCATTTGTTTTTTTATGGGGATTTTAGGTTCACGCCGGGTGGCGTTGACGtttttt                                                          >  2:14282/1‑149 (MQ=255)
                                                                                      gCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcgc                                                      <  1:639216/151‑1 (MQ=255)
                                                                                       cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCt                                                     <  2:318803/151‑1 (MQ=255)
                                                                                           gCTGATAAACCGTTTTCTGCTATGGCGGTTTACCGGGAGGAAAGGGGGGGAGGGTTAATTTCGTATTTGTTACCCGGGGGGCGTTGTCATTTTTTTTTTTATTGGGATTTTAGGGCCACCCTGGGTGAGGTTTTCGttttttt                                                         >  1:616386/1‑142 (MQ=255)
                                                                                              gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATaa                                                <  2:972848/149‑1 (MQ=255)
                                                                                              gATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGt                                              <  1:375807/151‑1 (MQ=255)
                                                                                               aTAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtt                                             <  1:149924/151‑1 (MQ=255)
                                                                                                   aCCGTTTTCTGCTATGGCGGTTACCCGGGGGAAAAAGGGGGGGGGGTACATTACGTATTTGTACCTGGGGGGGGATCGTCATTTTTTTTTTTATTGTGGTTTTCGGGCCACGCTGGGTGACGTTGACGTTTTTTTTGCTGATAAGtttttt                                         >  2:1156544/1‑149 (MQ=255)
                                                                                                     cGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTgg                                       <  2:155689/151‑1 (MQ=255)
                                                                                                        tttCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCa                                     <  2:1114225/150‑1 (MQ=255)
                                                                                                          tCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAtt                                   <  2:369859/150‑1 (MQ=255)
                                                                                                             gCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa                                 <  1:63117/149‑1 (MQ=255)
                                                                                                              cTATGGCGGTTACCGTAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGGTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGaaa                               <  2:960137/150‑1 (MQ=255)
                                                                                                                aTGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGaa                            <  2:224259/151‑1 (MQ=255)
                                                                                                                  ggCGGTTACCGGAGAGAAAAGGGGGGGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat                           >  1:159751/1‑150 (MQ=255)
                                                                                                                   gCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAatat                         >  1:447354/1‑151 (MQ=255)
                                                                                                                           aCCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                   <  1:413919/151‑1 (MQ=255)
                                                                                                                           aCCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                   <  2:835580/151‑1 (MQ=255)
                                                                                                                           aCCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                   <  2:956248/151‑1 (MQ=255)
                                                                                                                                 gagaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata             <  1:296563/151‑1 (MQ=255)
                                                                                                                                           ggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAtt   <  1:149929/151‑1 (MQ=255)
                                                                                                                                            gTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAttt  >  1:648963/1‑151 (MQ=255)
                                                                                                                                             |                                                                                                                                                        
AATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACCGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTT  >  NC_000913/1478185‑1478473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: