Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	43.7%	160.1 / 48.9	87	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (37/12);  new base A (0/38);  total (37/50)
Fisher's exact test for biased strand distribution p-value = 1.86e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29340‑29633                                                                                                                                                      |                                                                                                                                                    tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACaaa                                                                                                                                                  <  2:360345/150‑1 (MQ=255) tgttgtTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACaaa                                                                                                                                                  <  2:883287/150‑1 (MQ=255)  gttgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAaat                                                                                                                                                <  2:636746/151‑1 (MQ=255)    tgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCTTGTAAAACATGCAT‑GAG‑CCACAAaata                                                                                                                                               >  2:651891/1‑150 (MQ=255)     gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAaataat                                                                                                                                             >  1:107457/1‑151 (MQ=255)      ttttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAaataat                                                                                                                                             >  2:1038536/1‑150 (MQ=255)       tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAataata                                                                                                                                            <  1:1024839/150‑1 (MQ=255)       tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAataata                                                                                                                                            <  1:321351/150‑1 (MQ=255)       tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAatat                                                                                                                                           >  2:1021037/1‑151 (MQ=255)        ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAatat                                                                                                                                           <  2:489601/150‑1 (MQ=255)          aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaa                                                                                                                                         >  2:225427/1‑150 (MQ=255)             gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaaaaaa                                                                                                                                     <  2:450319/151‑1 (MQ=255)             gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaaaaa                                                                                                                                      >  1:1103466/1‑150 (MQ=255)              tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAAt                                                                                                                                    >  1:790703/1‑151 (MQ=255)               aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑TAG‑CCACAAAATAATATAAAAAAt                                                                                                                                    <  1:260116/150‑1 (MQ=255)               aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAAt                                                                                                                                    <  1:244459/150‑1 (MQ=255)                 aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATccc                                                                                                                                 <  2:650456/151‑1 (MQ=255)                   tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCg                                                                                                                                <  1:270186/150‑1 (MQ=255)                     tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGcc                                                                                                                              >  1:542436/1‑150 (MQ=255)                     tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGcc                                                                                                                              <  1:120629/150‑1 (MQ=255)                      gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATCCCTGTAAAACACGCAT‑GAG‑CCAAAAAATAATATAAAAAATCCCGCCa                                                                                                                             <  1:1153018/150‑1 (MQ=255)                          aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                          >  1:857805/1‑149 (MQ=255)                            ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                      >  1:178882/1‑151 (MQ=255)                              ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                       >  2:149487/1‑148 (MQ=255)                                 ccttttttttCTTCTCTTTTTTTTTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCC‑AAAACAGTTTAAATGATTTTTTTGTCGCTTAATGCCTGAAAAACAGGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGa                                                                                                                    <  1:1090976/144‑1 (MQ=255)                                     ttttttCTCTTTTTTTTTATTTCATGCATTTTTTTTTCTCCCCAAGCTTTTTCC‑AAAAAAGTTTAGATGATTTTTTTTTCGCTTAATCCCTATAAAA‑ATTGAT‑GGGCCCAAAAAAAAATATAAAAATCCCCGCCATTAAGTTGACtttt                                                                                                               <  1:528528/149‑1 (MQ=255)                                       ttttCTTCTTTTTTTTATTTCATCCAATCTTTTGCCTGCGCAAGCGTTTTCA‑AGAACAGTTTAAATATTTTTTTTGTCGTTAAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAgc                                                                                                            <  1:1132311/150‑1 (MQ=255)                                       ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                           >  1:1078698/1‑151 (MQ=255)                                               ttttttttATTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCA‑AGAACAGTTTAAATAATTTTTTTGTCGTTTAATGCCTATAAAACTTGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                        <  1:625311/143‑1 (MQ=255)                                                 tttttttttTCATGAATTTTTCTTGCTGCGCAAGTGTTTTCC‑AAAAAAAGTTAGTTGATTTTTTTGTCCGTTAATGCCTGAAAAACATGGAG‑GAG‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                      <  2:577308/145‑1 (MQ=255)                                                 gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                 >  1:114848/1‑151 (MQ=255)                                                  tttttATTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCC‑AAAACAGGTTAGATGATTTTTTTGTCTTTTAATCCCTGAAAAACATGCAT‑GAG‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                    <  2:679820/147‑1 (MQ=255)                                                  tttttATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCC‑AGAACAGTTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                  <  2:824391/149‑1 (MQ=255)                                                    ttttttTCATGCATTCTTCTTGCTGCGCAAGCTTTTTCA‑AGAACAGGTTAAATAATTTTTTTGTCGCTTAATGCCTTAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                               <  1:666518/150‑1 (MQ=255)                                                    ttttttTCATACACTCTTCTTGCTGCAAACCCGTTTTCC‑AAAACAGTTAAGAGCTTTTTTTTGTCGCTAAATGCCTAAAAAACATGCTT‑GAC‑CCCAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                               <  2:1132085/150‑1 (MQ=255)                                                           cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                       >  1:403174/1‑151 (MQ=255)                                                            aTGCATTCTTCTTGCTGCGCAGGTTTTTTCA‑AAAACGGTTTAGATATTTTTTTGGTCGCTTAACGCTTGAAAAACATAAAT‑GAC‑CCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                       <  2:662530/150‑1 (MQ=255)                                                             tGCATTTTTTTTGCTGCG‑AAGCCTTTTCCAAAAACAGGTTAGATTATTTTTTTGTCGTTAAATGCCTAAAAAACATGCAT‑GAC‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                        <  1:739213/148‑1 (MQ=255)                                                             tGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                     >  2:841459/1‑151 (MQ=255)                                                              ccatttttcttGTCGCGCAATCGTTTTCA‑AGAACAGTTTAAATAATTTTTTTGTCTTTAAACCCCTTAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                     <  1:966766/144‑1 (MQ=255)                                                                  tttttttGCTGCGAAAGCGTTTTCA‑AGACAGGGTTAGATAATTTTTTTGTCCATTAATGCAGGAAAAACATGCAT‑GAC‑CAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                 <  1:373741/145‑1 (MQ=255)                                                                         gctgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                          >  2:1085996/1‑150 (MQ=255)                                                                           tgcGCAAGCGTTTTCC‑AGAACCGGTTAGCTGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAGAGAATCCCGCCTTTAAGCTGACTTTTAGCGCCCATATCTCCAGACAGCCGCCGTTTGCCAGAAAt                                                                        >  1:529920/1‑150 (MQ=255)                                                                           tgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                       >  1:1045645/1‑151 (MQ=255)                                                                             cgcaggtttttcCC‑AAAACAGGTTAGATGTTTTTTTGTTCGATAAATGCCTTAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                       <  2:990662/141‑1 (MQ=255)                                                                             cgcAGGCTTTTTCA‑AGAACGGTTTAATTGTTTTTTTTGTCGTTTAATGCTTAAAAAACATGCAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                      <  2:822149/150‑1 (MQ=255)                                                                             cgcAAGCTTTTCCC‑AGAACAGGTTAGATGATTTTTTTGTCGCTTAATCCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                          <  2:381176/146‑1 (MQ=255)                                                                             cgcAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                     >  1:674295/1‑151 (MQ=255)                                                                               cAAGCGTTTTCC‑AGAACAGGGTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtagtc                                                                   >  1:1030015/1‑151 (MQ=255)                                                                                aggctttttCA‑AGAACAGTTTAGATGATTTTTTTGTCTTTTAATGCCTATAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                      <  1:535648/142‑1 (MQ=255)                                                                                aggctttttCA‑AAAACGGTTTAGATGTTTTTTTTGTCGCTTAATGCCTGAAAAACATGAAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                    <  2:904625/144‑1 (MQ=255)                                                                                 atcttttaCC‑AGAACGGGTTAGATAATTTTTTTGTCGCTAAATGCCTAAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                  <  1:799011/146‑1 (MQ=255)                                                                                              aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                     >  1:16480/1‑151 (MQ=255)                                                                                                   gTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                >  1:321504/1‑151 (MQ=255)                                                                                                    ttAAATGTTTTTTTTGCCGTTTAATGCCTGAAAAACATGCAG‑GAC‑CCAAAAAATAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  1:603203/150‑1 (MQ=255)                                                                                                    ttAAATGATTTTTTTTTCGTTTATTCCCTTAAAAACTTGCAT‑GCA‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  1:966424/150‑1 (MQ=255)                                                                                                    taaGATTATTTTTTTGGCTCTTAATGCTGGAAAAACAGGCAT‑GAC‑CCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                  <  2:565061/146‑1 (MQ=255)                                                                                                      aGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                             >  2:925958/1‑151 (MQ=255)                                                                                                         ttttttttttGTCGTTTACTCCTTGAAAAACATGCTG‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                           <  2:766971/145‑1 (MQ=255)                                                                                                         tgttttttttGTCGCTTAATGCCTAAAAAACATCCAT‑GAG‑CAAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                           <  2:1103503/145‑1 (MQ=255)                                                                                                              tttttGTCGCTTAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                       <  1:252182/149‑1 (MQ=255)                                                                                                               ttttGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                    >  1:218222/1‑151 (MQ=255)                                                                                                                  tGTCGTTAAATGCCTGAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                  <  2:96197/150‑1 (MQ=255)                                                                                                                   gTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                >  2:629719/1‑151 (MQ=255)                                                                                                                   gTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                >  2:593815/1‑151 (MQ=255)                                                                                                                    tCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                               >  2:914224/1‑151 (MQ=255)                                                                                                                        ttAATGCTTGAAAAACATGCAG‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                <  2:313911/146‑1 (MQ=255)                                                                                                                        ttAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           >  1:1113069/1‑151 (MQ=255)                                                                                                                        ttAATCCTTGAAAAACATGAAT‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                              <  2:372398/148‑1 (MQ=255)                                                                                                                         tAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          >  1:1121344/1‑151 (MQ=255)                                                                                                                          attccctaaaaaaCAGGCAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           <  2:848193/140‑1 (MQ=255)                                                                                                                          attGCTTGTAAAACATACAT‑GAG‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                             <  2:790728/145‑1 (MQ=255)                                                                                                                          atcgCCTGTAAAACA‑GCATAGAC‑CCAAAAAATATAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                            <  1:33227/145‑1 (MQ=255)                                                                                                                          aaTGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                          >  2:895884/1‑150 (MQ=255)                                                                                                                            tGCCTAAAAAACATGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTTCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           <  2:652806/147‑1 (MQ=255)                                                                                                                              ccaaaaaaaCAAGCGG‑GAG‑CAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           <  1:140462/140‑1 (MQ=255)                                                                                                                                   aaaaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 >  2:1019830/1‑150 (MQ=255)                                                                                                                                   aaaaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 >  1:298350/1‑150 (MQ=255)                                                                                                                                     aaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt               >  2:296434/1‑150 (MQ=255)                                                                                                                                        atgcag‑gGG‑CAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa            <  1:637458/150‑1 (MQ=255)                                                                                                                                          gcat‑gAG‑CCAAAAAATAATAAAAAAACCGCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa            <  2:982194/148‑1 (MQ=255)                                                                                                                                           cat‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg         >  1:720483/1‑150 (MQ=255)                                                                                                                                           cat‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACATCATCATTGTGAATTAATATGCAAATAAagtg         >  2:1057053/1‑150 (MQ=255)                                                                                                                                            at‑gCG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag       <  2:107464/151‑1 (MQ=255)                                                                                                                                            at‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt          >  2:568172/1‑148 (MQ=255)                                                                                                                                             t‑gAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga        <  1:79256/149‑1 (MQ=255)                                                                                                                                               gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCAAGAATGCCGCCGTTTGCCAGAAATTGGTCGGTAAGCAGATTTGAATGGATTTACGTCATCATTGGGAATTAATATGCAAATAAagtgagtg     >  1:164237/1‑151 (MQ=255)                                                                                                                                                    cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  2:1090985/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29340‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑