Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	45.2%	96.3 / 57.9	84	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (35/11);  new base A (0/38);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 2.63e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642                                                                                                                                                          |                                                                                                                                                   tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAatat                                                                                                                                                    >  2:1021037/1‑151 (MQ=255)  ttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAatat                                                                                                                                                    <  2:489601/150‑1 (MQ=255)    aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaa                                                                                                                                                  >  2:225427/1‑150 (MQ=255)       gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaaaaaa                                                                                                                                              <  2:450319/151‑1 (MQ=255)       gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATaaaaa                                                                                                                                               >  1:1103466/1‑150 (MQ=255)        tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAAt                                                                                                                                             >  1:790703/1‑151 (MQ=255)         aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑TAG‑CCACAAAATAATATAAAAAAt                                                                                                                                             <  1:260116/150‑1 (MQ=255)         aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAAt                                                                                                                                             <  1:244459/150‑1 (MQ=255)           aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATccc                                                                                                                                          <  2:650456/151‑1 (MQ=255)             tttGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCg                                                                                                                                         <  1:270186/150‑1 (MQ=255)               tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       >  1:542436/1‑150 (MQ=255)               tGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGcc                                                                                                                                       <  1:120629/150‑1 (MQ=255)                gACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATCCCTGTAAAACACGCAT‑GAG‑CCAAAAAATAATATAAAAAATCCCGCCa                                                                                                                                      <  1:1153018/150‑1 (MQ=255)                    aTTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                   >  1:857805/1‑149 (MQ=255)                      ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                               >  1:178882/1‑151 (MQ=255)                        ggTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGt                                                                                                                                >  2:149487/1‑148 (MQ=255)                           ccttttttttCTTCTCTTTTTTTTTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCC‑AAAACAGTTTAAATGATTTTTTTGTCGCTTAATGCCTGAAAAACAGGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGa                                                                                                                             <  1:1090976/144‑1 (MQ=255)                               ttttttCTCTTTTTTTTTATTTCATGCATTTTTTTTTCTCCCCAAGCTTTTTCC‑AAAAAAGTTTAGATGATTTTTTTTTCGCTTAATCCCTATAAAA‑ATTGAT‑GGGCCCAAAAAAAAATATAAAAATCCCCGCCATTAAGTTGACtttt                                                                                                                        <  1:528528/149‑1 (MQ=255)                                 ttttCTTCTTTTTTTTATTTCATCCAATCTTTTGCCTGCGCAAGCGTTTTCA‑AGAACAGTTTAAATATTTTTTTTGTCGTTAAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAgc                                                                                                                     <  1:1132311/150‑1 (MQ=255)                                 ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                    >  1:1078698/1‑151 (MQ=255)                                         ttttttttATTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCA‑AGAACAGTTTAAATAATTTTTTTGTCGTTTAATGCCTATAAAACTTGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGccc                                                                                                                 <  1:625311/143‑1 (MQ=255)                                           tttttttttTCATGAATTTTTCTTGCTGCGCAAGTGTTTTCC‑AAAAAAAGTTAGTTGATTTTTTTGTCCGTTAATGCCTGAAAAACATGGAG‑GAG‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                               <  2:577308/145‑1 (MQ=255)                                           gTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                          >  1:114848/1‑151 (MQ=255)                                            tttttATTTCATGCATTCTTCTTGCTGCGCAAGCTTTTCCC‑AAAACAGGTTAGATGATTTTTTTGTCTTTTAATCCCTGAAAAACATGCAT‑GAG‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                             <  2:679820/147‑1 (MQ=255)                                            tttttATTTCATGCAATCTTCTTGCTGCGCAAGCTTTTTCC‑AGAACAGTTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtct                                                                                                           <  2:824391/149‑1 (MQ=255)                                              ttttttTCATGCATTCTTCTTGCTGCGCAAGCTTTTTCA‑AGAACAGGTTAAATAATTTTTTTGTCGCTTAATGCCTTAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                        <  1:666518/150‑1 (MQ=255)                                              ttttttTCATACACTCTTCTTGCTGCAAACCCGTTTTCC‑AAAACAGTTAAGAGCTTTTTTTTGTCGCTAAATGCCTAAAAAACATGCTT‑GAC‑CCCAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCa                                                                                                        <  2:1132085/150‑1 (MQ=255)                                                     cATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                >  1:403174/1‑151 (MQ=255)                                                      aTGCATTCTTCTTGCTGCGCAGGTTTTTTCA‑AAAACGGTTTAGATATTTTTTTGGTCGCTTAACGCTTGAAAAACATAAAT‑GAC‑CCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccg                                                                                                <  2:662530/150‑1 (MQ=255)                                                       tGCATTTTTTTTGCTGCG‑AAGCCTTTTCCAAAAACAGGTTAGATTATTTTTTTGTCGTTAAATGCCTAAAAAACATGCAT‑GAC‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgcc                                                                                                 <  1:739213/148‑1 (MQ=255)                                                       tGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              >  2:841459/1‑151 (MQ=255)                                                        ccatttttcttGTCGCGCAATCGTTTTCA‑AGAACAGTTTAAATAATTTTTTTGTCTTTAAACCCCTTAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                              <  1:966766/144‑1 (MQ=255)                                                            tttttttGCTGCGAAAGCGTTTTCA‑AGACAGGGTTAGATAATTTTTTTGTCCATTAATGCAGGAAAAACATGCAT‑GAC‑CAAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                          <  1:373741/145‑1 (MQ=255)                                                                   gctgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   >  2:1085996/1‑150 (MQ=255)                                                                     tgcGCAAGCGTTTTCC‑AGAACCGGTTAGCTGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAGAGAATCCCGCCTTTAAGCTGACTTTTAGCGCCCATATCTCCAGACAGCCGCCGTTTGCCAGAAAt                                                                                 >  1:529920/1‑150 (MQ=255)                                                                     tgcGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                >  1:1045645/1‑151 (MQ=255)                                                                       cgcaggtttttcCC‑AAAACAGGTTAGATGTTTTTTTGTTCGATAAATGCCTTAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                <  2:990662/141‑1 (MQ=255)                                                                       cgcAGGCTTTTTCA‑AGAACGGTTTAATTGTTTTTTTTGTCGTTTAATGCTTAAAAAACATGCAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               <  2:822149/150‑1 (MQ=255)                                                                       cgcAAGCTTTTCCC‑AGAACAGGTTAGATGATTTTTTTGTCGCTTAATCCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaa                                                                                   <  2:381176/146‑1 (MQ=255)                                                                       cgcAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcg                                                                              >  1:674295/1‑151 (MQ=255)                                                                         cAAGCGTTTTCC‑AGAACAGGGTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtagtc                                                                            >  1:1030015/1‑151 (MQ=255)                                                                          aggctttttCA‑AGAACAGTTTAGATGATTTTTTTGTCTTTTAATGCCTATAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                               <  1:535648/142‑1 (MQ=255)                                                                          aggctttttCA‑AAAACGGTTTAGATGTTTTTTTTGTCGCTTAATGCCTGAAAAACATGAAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                             <  2:904625/144‑1 (MQ=255)                                                                           atcttttaCC‑AGAACGGGTTAGATAATTTTTTTGTCGCTAAATGCCTAAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgtcg                                                                           <  1:799011/146‑1 (MQ=255)                                                                                        aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                              >  1:16480/1‑151 (MQ=255)                                                                                             gTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         >  1:321504/1‑151 (MQ=255)                                                                                              ttAAATGTTTTTTTTGCCGTTTAATGCCTGAAAAACATGCAG‑GAC‑CCAAAAAATAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  1:603203/150‑1 (MQ=255)                                                                                              ttAAATGATTTTTTTTTCGTTTATTCCCTTAAAAACTTGCAT‑GCA‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                         <  1:966424/150‑1 (MQ=255)                                                                                              taaGATTATTTTTTTGGCTCTTAATGCTGGAAAAACAGGCAT‑GAC‑CCAAAAAAAAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                           <  2:565061/146‑1 (MQ=255)                                                                                                aGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGAtt                                                      >  2:925958/1‑151 (MQ=255)                                                                                                   ttttttttttGTCGTTTACTCCTTGAAAAACATGCTG‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  2:766971/145‑1 (MQ=255)                                                                                                   tgttttttttGTCGCTTAATGCCTAAAAAACATCCAT‑GAG‑CAAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTa                                                    <  2:1103503/145‑1 (MQ=255)                                                                                                        tttttGTCGCTTAATGCCTGAAAAACATGCAT‑GAG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtc                                                <  1:252182/149‑1 (MQ=255)                                                                                                         ttttGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                             >  1:218222/1‑151 (MQ=255)                                                                                                            tGTCGTTAAATGCCTGAAAAACATGCAT‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                           <  2:96197/150‑1 (MQ=255)                                                                                                             gTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         >  2:593815/1‑151 (MQ=255)                                                                                                             gTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         >  2:629719/1‑151 (MQ=255)                                                                                                              tCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgt                                        >  2:914224/1‑151 (MQ=255)                                                                                                                  ttAATGCTTGAAAAACATGCAG‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                         <  2:313911/146‑1 (MQ=255)                                                                                                                  ttAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    >  1:1113069/1‑151 (MQ=255)                                                                                                                  ttAATCCTTGAAAAACATGAAT‑GAC‑CCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                       <  2:372398/148‑1 (MQ=255)                                                                                                                   tAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  1:1121344/1‑151 (MQ=255)                                                                                                                    attccctaaaaaaCAGGCAT‑GAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    <  2:848193/140‑1 (MQ=255)                                                                                                                    attGCTTGTAAAACATACAT‑GAG‑CCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                      <  2:790728/145‑1 (MQ=255)                                                                                                                    atcgCCTGTAAAACA‑GCATAGAC‑CCAAAAAATATAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                     <  1:33227/145‑1 (MQ=255)                                                                                                                    aaTGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                   >  2:895884/1‑150 (MQ=255)                                                                                                                      tGCCTAAAAAACATGCAT‑GAC‑CCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTTCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    <  2:652806/147‑1 (MQ=255)                                                                                                                        ccaaaaaaaCAAGCGG‑GAG‑CAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                    <  1:140462/140‑1 (MQ=255)                                                                                                                             aaaaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                          >  1:298350/1‑150 (MQ=255)                                                                                                                             aaaaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                          >  2:1019830/1‑150 (MQ=255)                                                                                                                               aaCATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAAt                        >  2:296434/1‑150 (MQ=255)                                                                                                                                  atgcag‑gGG‑CAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                     <  1:637458/150‑1 (MQ=255)                                                                                                                                    gcat‑gAG‑CCAAAAAATAATAAAAAAACCGCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                     <  2:982194/148‑1 (MQ=255)                                                                                                                                     cat‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                  >  1:720483/1‑150 (MQ=255)                                                                                                                                     cat‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACATCATCATTGTGAATTAATATGCAAATAAagtg                  >  2:1057053/1‑150 (MQ=255)                                                                                                                                      at‑gCG‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                <  2:107464/151‑1 (MQ=255)                                                                                                                                      at‑gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   >  2:568172/1‑148 (MQ=255)                                                                                                                                       t‑gAC‑CCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                 <  1:79256/149‑1 (MQ=255)                                                                                                                                         gAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCAAGAATGCCGCCGTTTGCCAGAAATTGGTCGGTAAGCAGATTTGAATGGATTTACGTCATCATTGGGAATTAATATGCAAATAAagtgagtg              >  1:164237/1‑151 (MQ=255)                                                                                                                                             caaaaaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt               <  2:768728/143‑1 (MQ=255)                                                                                                                                              cacaAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat           >  2:1090985/1‑150 (MQ=255)                                                                                                                                               aaaaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata          <  2:1114472/148‑1 (MQ=255)                                                                                                                                               aaaaaaTAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                   <  1:4895/139‑1 (MQ=255)                                                                                                                                                aaaaaTAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  1:811647/150‑1 (MQ=255)                                                                                                                                                   aataatATAAAAAATCCCGCCATTAAGTTGTCTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt        <  2:206695/148‑1 (MQ=255)                                                                                                                                                     taataTAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg   >  1:1124366/1‑151 (MQ=255)                                                                                                                                                       atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  2:857401/1‑150 (MQ=255)                                                                                                                                                        tataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg  >  1:1171449/1‑149 (MQ=255)                                                                                                                                                          |                                                                                                                                                   TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCC‑AGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCAT‑GAG‑CCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGG  >  NC_000913/29346‑29642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑