Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	46.2%	60.2 / 44.8	65	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (5/30);  new base T (30/0);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.94e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583                                                                                                                                                      |                                                                                                                                                     gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCa                                                                                                                                                    <  1:18166/151‑1 (MQ=255)   gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                                  <  2:447827/151‑1 (MQ=255)     aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGTCa                                                                                                                                                <  1:457257/151‑1 (MQ=255)       ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACaa                                                                                                                                               >  2:47321/1‑150 (MQ=255)         cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACAAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATa                                                                                                                                            <  2:388122/151‑1 (MQ=255)          gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAATa                                                                                                                                            >  2:468281/1‑150 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACa                                                                                                                                          <  2:158030/151‑1 (MQ=255)           cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCGAaaaaaa                                                                                                                                            >  1:1140915/1‑147 (MQ=255)            aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaa                                                                                                                                         <  1:796586/151‑1 (MQ=255)             aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaa                                                                                                                                        <  1:817232/151‑1 (MQ=255)                cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAAc                                                                                                                                     <  1:911229/151‑1 (MQ=255)                   tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTAGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATg                                                                                                                                   >  2:258210/1‑150 (MQ=255)                    gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTgg                                                                                                                                  <  2:1116618/150‑1 (MQ=255)                          cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAAAAAAAAAAAATGGAGGTGcc                                                                                                                           >  2:739109/1‑151 (MQ=255)                            cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTTACACAAAAAAAAAAAATGGAGt                                                                                                                               >  1:122071/1‑145 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCACACAAATAAAAAAAATGGAGTTGCCat                                                                                                                         >  2:413747/1‑149 (MQ=255)                                 ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGAGTTGCCatga                                                                                                                       >  1:1074058/1‑148 (MQ=255)                                 ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAACAAAAAATTGGGTTGCCatg                                                                                                                        >  1:47523/1‑147 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                             <  2:664669/151‑1 (MQ=255)                                         atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGt                                                                                                            <  1:637938/151‑1 (MQ=255)                                           attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCG‑AAATTTTTCAAAAAAAAAAAAAAAATGGGGTTTCCCTGTTGAGCGaaa                                                                                                              >  1:998308/1‑147 (MQ=255)                                              cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTCCACAAATACAAAAAACAGAGCTGCCACTTTTAGGGAAAGcaata                                                                                                        >  2:881248/1‑150 (MQ=255)                                                 tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCCTACAAAAAAAAAAAAATGGGGTGGCAAGATGAGGGAAAGaaataaaa                                                                                                     >  1:147809/1‑148 (MQ=255)                                                  cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCTGACAAAAAAAAAAAATGGAAATTTCATGATGAGGGGAAGgaaaaaa                                                                                                      >  2:503093/1‑147 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAAAAAAATACAAAAAATAGAGTTGTCATTATGAGGGAAAAAAATAAACaa                                                                                                   >  1:1018777/1‑149 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                    <  1:58478/148‑1 (MQ=255)                                                     aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                 <  2:477364/150‑1 (MQ=255)                                                       tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AAATTTTTTAGACAAAAACAAAAAAAAGAGGTGTCATGATTAGCGGAAATAAAAAACaaaa                                                                                                 >  1:949544/1‑146 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGGGTTTCCATGATGAGGGAAAGAAATAAGCAACaag                                                                                               >  1:556096/1‑145 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGGGGGAAAGGAAAAAacaacaag                                                                                               >  2:1139606/1‑145 (MQ=255)                                                               tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                                       <  2:844117/150‑1 (MQ=255)                                                                  ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATTGAGGTGGCATGATGAGGGGAAGTAAAAAAAAAAAAGCAGGGAATaa                                                                                     >  2:237717/1‑149 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAACAAGGGGTGGCCTTATGGGCGAAAATAAAAAACAAAAAGCAGGGAATaa                                                                                     >  1:183223/1‑148 (MQ=255)                                                                    cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCACACAAATAAAAAAAACAGGGGTGTCATGTTGAGCGCAAGGAAAAAGAAAAACGCAGTGAAAAAGGTg                                                                                 >  1:589492/1‑151 (MQ=255)                                                                         aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACaga                                                                            <  1:207845/151‑1 (MQ=255)                                                                           tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGAAAAAAACAAAAAATGGAGGTGTCATGATGAGGGAAAGGAATAAACAAAAAGCAGGGAATAAATTGAAag                                                                             >  2:342/1‑148 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                           <  1:165581/149‑1 (MQ=255)                                                                            gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag                                                                           <  2:392715/149‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGAAAAAAAAAAAAAATGGAGTTGCCATGATTAGCGAAAATAATAAACAAAAAGCAGGGAATAAAATGACAGAGAtt                                                                        >  2:340157/1‑150 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                       <  1:652078/150‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCt                                                                  <  2:1078042/151‑1 (MQ=255)                                                                                   aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGTTTGCCTGATGAGGGGAAAGAATAAAAAAAAAGCAGTGAATAAAATGACAGAGATTGt                                                                      >  1:1057121/1‑147 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAAc                                                               <  2:680341/150‑1 (MQ=255)                                                                                            cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGATGAGGGGAAGGAATAAAAAACAAGCAGGGAATAAGATGACAGAGATTGTCGCTTACttt                                                            >  2:66754/1‑148 (MQ=255)                                                                                             ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTGCCATGATTAGCGGAAGGAATAAACAAAAAGCAGGGAATAAATTGGAAGAGAATTTCGCTAACttt                                                            >  2:742036/1‑147 (MQ=255)                                                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                                     <  2:406996/151‑1 (MQ=255)                                                                                                 gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCA‑ACAAAAAAAAAAAATGGAGGTGCCCTGATGAGGGGAAAAAAAAAACAAAAAGCAGTGAATAAAATTACAGAGATTTTTGCTAAATTTTCCGcc                                                      >  2:610514/1‑149 (MQ=255)                                                                                                    aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGGGGTGCCCTGATGAGCGGAAGAAATAAACAACAAGCCGGGAATAAGTTGAAAGAGATTTTTGCTAACTTTACCGCCCTg                                                   >  2:448054/1‑149 (MQ=255)                                                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAATAAAAAAAATGGAGGTGGCCTGATGAGCGAAAGGAATAAACAAAAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCGCCATGAtt                                                >  2:370391/1‑151 (MQ=255)                                                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAAGGATTGTCGCTAACTTTACCGCCATGAtt                                                <  1:540050/151‑1 (MQ=255)                                                                                                          ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAAAGGAGTTGCCATGATGAGGGGAAAGAATAAACAAAAAGCAGGGAATAAGATGAAAGAGAATGTCGCTAACTTTAACCCCATGAttttt                                             >  2:1194713/1‑147 (MQ=255)                                                                                                          aaaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCAACCTTTACCGCCATGATTTCTAc                                           <  1:408931/149‑1 (MQ=255)                                                                                                                ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                      <  2:695063/150‑1 (MQ=255)                                                                                                                       tttAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAACAAAAAATTGAGTTTCCATGATGGGCGGAAATAATAAAAAAAAAGCAGTGAAAAAATTGAAAGAGATTTTCGCCTAATTTTCCCCCACGATTTTTAacc                                         >  2:927377/1‑140 (MQ=255)                                                                                                                            tGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAACGGAGGTGGCATGATGAGCGAAAGGAATAAACAAAAGGCAGTGAATAAGTTGAAAGAGAATGTCGCTAACTTTTCCGCCCTGATTTTTTCCCGAATGCCTGATGACg                          >  2:183608/1‑150 (MQ=255)                                                                                                                             ggTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAACCGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACg                          <  2:499647/149‑1 (MQ=255)                                                                                                                             ggTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                         <  1:314618/150‑1 (MQ=255)                                                                                                                                     gggggggTG‑AATTTTTCAAGACAAATACAAAAACTGGAGTGGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa                 <  1:569225/145‑1 (MQ=255)                                                                                                                                      tgGCGGTG‑AATTTTTCGAAAAAAATACAAAAGCGGGAGTGCCCATATTGAGCGAAAGTAAAAAGCACAAGGCAGTGAATAATTTGACAGAGATGGTGGTGAGGTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                <  1:133251/150‑1 (MQ=255)                                                                                                                                       ggCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc              >  2:896961/1‑151 (MQ=255)                                                                                                                                         gggTG‑ATTTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGAGGAGCGAAAGTAATAAGCAACGGGCAGTGAATAAGTTGACAGGGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa            <  1:140658/150‑1 (MQ=255)                                                                                                                                             g‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:1098058/1‑151 (MQ=255)                                                                                                                                                  ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  2:201828/1‑151 (MQ=255)                                                                                                                                                   tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    >  1:315916/1‑150 (MQ=255)                                                                                                                                                   tttCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt    <  2:882744/150‑1 (MQ=255)                                                                                                                                                      ccAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  1:496349/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                     GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑