Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,079	0	C	T	43.9%	73.0 / 45.6	66	L134L (CTG→CTA)	thiB	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (6/31);  new base T (29/0);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 3.98e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTT  >  NC_000913/74935‑75228                                                                                                                                                  |                                                                                                                                                           ggCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑ttt                                                                                                                                                      <  1:985952/150‑1 (MQ=255)      gtgGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGTTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCt                                                                                                                                                 <  1:902601/150‑1 (MQ=255)        ggggCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTg                                                                                                                                               <  2:232548/150‑1 (MQ=255)          ggCGTCATCGCCCTATCTTTTTTGCACCCATACCAACAGCCCCAGCCCCGTTGTACTGGTGCGCGAATCCTGAAAAACCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCa                                                                                                                                            <  1:638369/151‑1 (MQ=255)          ggCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCa                                                                                                                                            <  2:748723/151‑1 (MQ=255)            cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑Ta                                                                                                                                         <  2:1079380/151‑1 (MQ=255)            cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTGTtttttttat                                                                                                                                           >  1:43393/1‑145 (MQ=255)              tCATCGCCATAGACTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑Taa                                                                                                                                        <  1:1095860/150‑1 (MQ=255)              tCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAA‑TTTGTTTTTTTCA‑tta                                                                                                                                        >  1:1198298/1‑148 (MQ=255)               cATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTTTTTTTGTCA‑Taaa                                                                                                                                       >  2:648531/1‑150 (MQ=255)               cATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑Taaa                                                                                                                                       <  1:797048/150‑1 (MQ=255)                aTCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑Ttttttttttttttaaa                                                                                                                                        >  2:17802/1‑141 (MQ=255)                  cGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑aa                                                                                                                                  <  2:673721/151‑1 (MQ=255)                  cGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTCGTGGCGGG‑TTTTTTAG‑TTTTTTTTTGTCA‑Taaa                                                                                                                                       >  2:38449/1‑147 (MQ=255)                    ccATAGCCTTTTTGCATCCATAGCAACAGCCCCAGCCCCGGTGTACTGGCGCGCGGATCCTGATAAAGCCTCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAg                                                                                                                                 <  2:654011/150‑1 (MQ=255)                    ccATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAg                                                                                                                                 <  1:665114/150‑1 (MQ=255)                    ccATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTTTTTTTTTTA‑TTAAAG‑aa                                                                                                                                  >  2:800550/1‑149 (MQ=255)                      aTAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTG‑Ttttttttttttcttaaagaa                                                                                                                                    >  1:711753/1‑135 (MQ=255)                         gACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTC‑TTTTTTTTTTTTA‑TAAAAG‑AAAGCg                                                                                                                              >  2:1069060/1‑148 (MQ=255)                         gACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTGTttttttttcaaacaaa                                                                                                                                    >  2:238173/1‑132 (MQ=255)                         gACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAg                                                                                                                           <  2:889774/151‑1 (MQ=255)                          aCTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTCAG‑GTTTTTTTTTTTA‑TAAAAA‑AAAGCGAAg                                                                                                                           >  2:73508/1‑150 (MQ=255)                            tttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTG‑TTTTTTCTTTTTC‑TATAAG‑AAGGGGAAg                                                                                                                           >  1:68096/1‑148 (MQ=255)                             ttttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAg                                                                                                                        <  2:425619/150‑1 (MQ=255)                               ttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGcc                                                                                                                      <  2:817719/150‑1 (MQ=255)                                       aTAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGACCCTGATAAACCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCaaa                                                                                                             <  1:228473/151‑1 (MQ=255)                                           cAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACgg                                                                                                          <  2:710841/150‑1 (MQ=255)                                            aaCAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAG‑GTTTTTTTTTTTC‑TAAACG‑AAAGCGGAGTAAGCCCAATCaaaaag                                                                                                          >  1:54216/1‑146 (MQ=255)                                              cAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTGTTTTTTTTT‑TAAAAC‑AAGGGGAAGAAGACACAATaaaa                                                                                                             >  1:684360/1‑144 (MQ=255)                                                 aCCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTG‑TTTGTTTTTTTTA‑TATACG‑AAAGCGAAAGAGGCCAAATCAAACGGGACGaa                                                                                                    >  1:653095/1‑150 (MQ=255)                                                  cccAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTTTTTTTTTTC‑TAAAAA‑AAGGCGGAGGAGCCCTAATTAAACAGTaagca                                                                                                    >  1:175756/1‑145 (MQ=255)                                                         cgggtttgcTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa                                                                                             <  1:269539/141‑1 (MQ=255)                                                         ccGTTGTTCTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa‑tt                                                                                          <  2:769904/151‑1 (MQ=255)                                                          cGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa‑tta                                                                                         <  2:850083/151‑1 (MQ=255)                                                           ggTGTACTGGTGCGCGGATCCTGATAAATCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCa‑ttat                                                                                        <  1:1076180/151‑1 (MQ=255)                                                            gtgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTTTTTTTTTTA‑TAAAAA‑AAAGGGAAATAGCCAAAATAAAACAGGACGAAAAAGTCa‑ttttt                                                                                       >  2:854141/1‑151 (MQ=255)                                                              gtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTc                                                                                      <  1:284527/150‑1 (MQ=255)                                                                   ggTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAg                                                                                   <  2:1134895/148‑1 (MQ=255)                                                                   ggTGCGCGGATCCTGAAAAATCGCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAgccg                                                                                <  2:488013/151‑1 (MQ=255)                                                                         cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTGTTTTTTTTA‑TTAACG‑AAAGCGAAGAAGGCATAATCAAACGGGACGAAAGGGTCA‑TTATTTCAGCCGCC‑ggg                                                                          >  1:371421/1‑150 (MQ=255)                                                                           gATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTTCTG‑TTT‑CTCTTGTTT‑TAACCA‑AAGATGAAGCAGCCCTTATCAAACAGTGCGTGAAAGTCG‑GTATTTccgccgcc‑gcgga                                                                        >  1:183952/1‑146 (MQ=255)                                                                              cttaaaaaaTCCCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAAc                                                                       <  1:17802/142‑1 (MQ=255)                                                                               cTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAG‑TTTTTTTTTTTTA‑TAAAAA‑AAAGGGGAGTAGGCATAATCAAACGGGACGAAAAGGTCA‑ATATTTCAGCCGCC‑GGGGACGtt                                                                    >  2:888062/1‑150 (MQ=255)                                                                                tGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTG‑TTTGTTTTTTTTA‑TAAACA‑AAGGCGGAGAAGCCCTAATTAAAAGGGACGAAAAAGTCA‑TTTTTCCAGCCGCC‑GGGGACGtt                                                                    >  1:264854/1‑149 (MQ=255)                                                                                         aCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAG‑TTTTTTTTTTTCC‑TAAACG‑AAGGCGAAGGAGGCATTATCAAAAGGTACGAAAATGTCA‑TTTTTTCCGCCGCC‑CGGGACGGTAAAGGCATcc                                                          >  1:421131/1‑150 (MQ=255)                                                                                         aCCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAG‑TTTTTTTTTTTCA‑TAAAAG‑AAGGCGAAGGAGACAAAATCAAAAGGGAAGAAAGAGGCA‑TTATTTCAACCGCC‑GGGGACGGTAACGGCATcc                                                          >  2:483389/1‑150 (MQ=255)                                                                                           ccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTTTTTTTTTTATTAAACA‑AAGG‑GGAGGAGGCATAAACAAAAGGTACGAAAGGGGCATTTTTTCCACCGCCC‑GGGAAGTTTAAC‑GCATCCGc                                                        >  2:201604/1‑150 (MQ=255)                                                                                             gCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAG‑TTTTTTTTTTTCA‑TAAACG‑AAAGGGGAGGAGGCCTAATCAAAAGGGACGAAAAGGGTA‑TTATTTCCGCCGCC‑CGGGACGTTTACGGCATcccc                                                        >  2:64086/1‑146 (MQ=255)                                                                                              ccAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTCTG‑TTTTTTTTTTTTA‑TAAAAG‑AAGGGGAAGAAGCCATAATCAAAAAGGACGAAAGTGTCA‑TTTTTTCCGCCCCC‑GGGGACGGTTACGGCATCCCCCGcc                                                    >  2:992304/1‑151 (MQ=255)                                                                                               cAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTG‑TTTGTTTTTGTTA‑TAAAAG‑AAAGGGAAGGAGCCCAAATCAAACGGGACGGAAGAGTTA‑TTATTTCCGCCGCCGGGGAAGGTTAA‑GGCATCCCCTGcc                                                    >  2:218987/1‑150 (MQ=255)                                                                                                aGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTTTTTTTTTTA‑TAAAAGAAAGGGGAAG‑AGACATAAACAAAAGGGACGAAAAGGTCA‑TTATTTCAACCCCC‑CGGGAAGTTTAAAGCATCCGCTGcccc                                                  >  2:442170/1‑149 (MQ=255)                                                                                                 gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCcaca                                                 <  1:930435/151‑1 (MQ=255)                                                                                                       aTCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCtt                                           <  1:59845/151‑1 (MQ=255)                                                                                                        tCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTAC‑TTTTTTTTTTTCA‑TAAAAC‑AAAGCGGAGAAAGCATAAATAAAAAGGACGAAAAAGTCA‑TTATTTCAACCGCC‑GGGGACGTTTAAGGCATCCCCCGCCACAccccctt                                          >  2:1052925/1‑146 (MQ=255)                                                                                                          gCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTTGTTTTTTTTTTTTCT‑AAAAAA‑AAGGGGAAG‑AACCATAACTAAACCGGAAGAAAGGGTTG‑TTATTTTAGCCGCC‑GGGGAAGGTAAACGCAACCTCTGCCACCCCCCcttt                                         >  2:147270/1‑150 (MQ=255)                                                                                                             ctcAACCAGTTCTTTCAGGCTTTGTGGCGGG‑GTTTTTTG‑TTTTTTTTTTTTA‑TAAAAA‑AAAGGGGAGGAGGCCTAATCAAAAGGGACGAAAAAGTCA‑TTATTTCAGCCGCC‑CGGGAAGGTAAAGGCATCCCCCGcccccccccctttt                                        >  2:359957/1‑136 (MQ=255)                                                                                                                 aCCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTTTTTTTTTCC‑TAAAAG‑AAAGGGAAGTAGCCATAATCAAAAGGGACGAAAAGGGCA‑TTATTTCAGCCGCC‑GGGGACGTTAACGGCATCCGCCGCCACACCCCCTTTTGCaaa                                   >  2:795433/1‑149 (MQ=255)                                                                                                                     gTTCTTTCAGGCTTTGTGGCGGG‑TTTTTTAG‑TTTTTTTTTTTTA‑TAAAAA‑AAAGCGAAATAGGCATAATTAAAAGGGACGAAAAAGTCA‑TTATTTTAGCCCCC‑CGGGACGGTAACGGCATCCCCCCCCCCACCCCCTTTTGCAAAAAATcc                             >  2:705899/1‑151 (MQ=255)                                                                                                                          ttCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGtt                         <  1:133363/150‑1 (MQ=255)                                                                                                                                   tgtgGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                <  2:789816/150‑1 (MQ=255)                                                                                                                                      ggCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGgcggcg             <  2:1051380/150‑1 (MQ=255)                                                                                                                                       gCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTc           <  1:610000/151‑1 (MQ=255)                                                                                                                                           g‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAAc       <  1:1075039/151‑1 (MQ=255)                                                                                                                                             tttttCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAAc       >  1:1131907/1‑150 (MQ=255)                                                                                                                                                ttCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGgt   >  1:288744/1‑151 (MQ=255)                                                                                                                                                ttCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGgt   >  2:828405/1‑151 (MQ=255)                                                                                                                                                  cAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGgtt  <  2:315102/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                           GGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGG‑TTTTTCAG‑TTTGTTCTTGTCA‑TAAACG‑AAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCA‑TTATTCCAGCCGCC‑GGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTT  >  NC_000913/74935‑75228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑