Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,1090AC44.9% 148.7 / 37.7 100G219G (GGT→GGG) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base A (44/10);  new base C (0/44);  total (44/56)
Fisher's exact test for biased strand distribution p-value = 1.58e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537962‑4538258
                                                                                                                                                         |                                                                                                                                                        
ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑cc                                                                                                                                                       <  2:294623/150‑1 (MQ=255)
ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑c                                                                                                                                                        <  2:1104137/149‑1 (MQ=255)
 cccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑tt                                                                                                                                                    >  1:246236/1‑151 (MQ=255)
  ccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGCT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑ttt                                                                                                                                                   <  1:865073/151‑1 (MQ=255)
   cGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTa                                                                                                                                                  <  1:290632/151‑1 (MQ=255)
      aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTAtt                                                                                                                                                >  2:585405/1‑150 (MQ=255)
       aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTc                                                                                                                                               >  1:127028/1‑150 (MQ=255)
        aaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTcac                                                                                                                                             <  1:70912/151‑1 (MQ=255)
             ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccg                                                                                                                                       <  1:46033/151‑1 (MQ=255)
             ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccg                                                                                                                                       <  2:931036/151‑1 (MQ=255)
             ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑cc                                                                                                                                        <  1:441088/150‑1 (MQ=255)
               aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccgcc                                                                                                                                     >  1:115052/1‑151 (MQ=255)
                   aCGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCAc                                                                                                                                 >  2:751546/1‑151 (MQ=255)
                   aCGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCAc                                                                                                                                 <  2:694378/151‑1 (MQ=255)
                       cATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGc                                                                                                                             >  1:7457/1‑151 (MQ=255)
                         tcgggTGATGAGCGGGGAGCAATTTTTTTCAATTTTAAATTATTCCGGGT‑AAAATAAAGTTCAAATC‑CGGCCCCCGTC‑CGCAATCCTGGTTTGGCTTCGCCATAAATAAG‑CCAGG‑TTTT‑ATCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGc                                                                                                                             <  1:110230/146‑1 (MQ=255)
                            ggggatgaGACGGGAACAAGTTTTTCCCTTTTTAAATTTTTCCCGTT‑AAAATAAAGTTAAAATC‑CGGCATCCGTT‑CCCAATCCTGTTTTGGCTCCGCCATTAAAAAC‑CCAGT‑TTTT‑ATCC‑CT‑TTTTTCCACAA‑CCGCCGCACCAGCCg                                                                                                                           <  1:722129/145‑1 (MQ=255)
                                atggGACGGGAGCAAGTTTTTTCCTTTTAAAATTTTTCCGGGT‑AAAAAAAAGTTAAAATC‑CGCCCCCCTTC‑CGAAATCCTGGTTGGCCTCCGCCTTAAAAAGG‑CCGGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGt                                                                                                                     <  1:146901/150‑1 (MQ=255)
                                 tgaGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTAc                                                                                                                   >  1:795023/1‑151 (MQ=255)
                                        ggAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAg                                                                                                                >  2:630943/1‑147 (MQ=255)
                                             aaGTTTATTCCATTTAAAATTATTCCCGGT‑AAAATCAATTTCAAATA‑CGGCATCCGTT‑CGCAACCCTGGTTTGGCTTCCCCATTAATAAG‑CCGGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                        <  2:818752/150‑1 (MQ=255)
                                              aGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                      >  1:863117/1‑151 (MQ=255)
                                              aGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                      >  1:1174796/1‑151 (MQ=255)
                                               gCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     >  1:331453/1‑151 (MQ=255)
                                                 ttATTCCTTTTTAATTTATTCCGGGT‑AAAATCAATTTCAATTA‑CGGCATCCGTT‑CGCAACCCTGTTTTGGCTCCCCCTTTAAAAAC‑CCAGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                     <  2:790439/149‑1 (MQ=255)
                                                    ttCTATTTAAAATTATT‑CCGGTAAAAATAAAGTTAAAATC‑CGCCCCCCTTC‑CGCACCCTTGTTTTGGCTCCCCCTTAAATACC‑CCGGT‑TTTT‑ACCC‑CC‑TTTTCCCCAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                   <  1:655913/148‑1 (MQ=255)
                                                    ttCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                >  1:464753/1‑151 (MQ=255)
                                                    ttCCATTTAAAATTATTCCCGGT‑AAAATCAGGTTAAAATC‑CGGCATCCGTT‑CGAAATCCTGTTTTGGTTCCGCAATAAAAAAG‑CCGGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                                  <  2:21509/149‑1 (MQ=255)
                                                    tcctaTTTTAATTTATTCCCGTT‑AAATTAATTTTCAAATA‑CGGCTTCCTTT‑CGCAATCTGGGTTTGTTTTCCCAATTAATAAG‑CCGGT‑TTTT‑ATCT‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                   <  2:534026/144‑1 (MQ=255)
                                                     tCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                >  2:337421/1‑150 (MQ=255)
                                                         tttaAAATTATCCCCGGG‑GAAAGCAGCTACAAATC‑CGCCATCCTTT‑CCAAATCCTGGTTTGCCTCCGCCATTAATAAG‑CCGGG‑TTTT‑TTCC‑CT‑TTTATCCACAG‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                   <  2:259668/143‑1 (MQ=255)
                                                          tttAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                          >  2:1148544/1‑151 (MQ=255)
                                                          tttAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                          >  1:998046/1‑151 (MQ=255)
                                                             aatttatcccgggT‑AAAACCAAGTTAAAATC‑CGGCATCCTTT‑CGAATCCCTGTTTTGGTTTCGCCATTAAAAAG‑CCAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                       <  2:664430/140‑1 (MQ=255)
                                                             aaaTTATTCCCGTT‑AAAATCAAGTAAAATTA‑CGCCACCCGTC‑CGCAATCTTGGTTTGGCTTCCCCATTAATAAC‑CCGGT‑TTTT‑ACCC‑CC‑TTTATCCAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                       <  2:321176/151‑1 (MQ=255)
                                                             aaaTTATTACGGGT‑AAAATAAATTTAAAATA‑CGCCATCCTTT‑CGCAATCCTGTTTGGGCTCCGCCATAAATAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                       <  2:793566/151‑1 (MQ=255)
                                                             aaaTTATCCCGGGT‑AAAATCAA‑TTTAAATC‑CCGCCCCCGTTCCCCAACCCTGGTTTGCTTTCCCAATAAAAAAG‑CC‑GG‑TTTT‑AACC‑CCTTTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCa                                                                                         <  1:1008475/149‑1 (MQ=255)
                                                              aaTTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                      >  2:690750/1‑151 (MQ=255)
                                                              aaTTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                      >  2:294053/1‑151 (MQ=255)
                                                               attattACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                                                                                     >  2:619638/1‑151 (MQ=255)
                                                                    taCCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                                >  1:616417/1‑151 (MQ=255)
                                                                     aCCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                               >  2:493911/1‑151 (MQ=255)
                                                                      ccGGT‑AAAATAAAGTTAAAATA‑CGGCATCCTTT‑CGCAATCCTGGTTTGTCTCCGCCATAAAAAAG‑CCGGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                               <  1:1014424/150‑1 (MQ=255)
                                                                       cGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                              >  1:863168/1‑150 (MQ=255)
                                                                         gT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAgg                                                                           >  2:36878/1‑151 (MQ=255)
                                                                          t‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGACTTCGCCATTAATAAG‑CCAGG‑CTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                               >  1:520794/1‑146 (MQ=255)
                                                                            gAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                          >  2:1154208/1‑150 (MQ=255)
                                                                             aaaTCAAGTTAAAATC‑CGGCATCCTTT‑CGCAATCCTGTTTTGGCTTCGCCATTAATAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                        <  2:128877/151‑1 (MQ=255)
                                                                             aaaTCAAGTTAAAATA‑CGGCACCCGTT‑CGCAATCCTGGTTTGGTTTCGCCATTAATAAG‑CCAGT‑TTTT‑ACCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                        <  2:181376/151‑1 (MQ=255)
                                                                              aaaccaattCAAAAACCGGCATCCGTT‑CGCATCCCGGGTTTGCCTCCGCCATAAAAAAC‑CCAGG‑TTTA‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                        <  1:616292/144‑1 (MQ=255)
                                                                               acaaatttaaactA‑CGGCACCCGTC‑CGCAACCCTGTTTGGGCTCCGCCATAAAAAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTACCCCAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                       <  1:337987/138‑1 (MQ=255)
                                                                                 aaattaaaaaTG‑CGGCACCCGTT‑CCCAACCCGGTTTTGTCTTCCCCATTAAAAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                     <  1:1154759/143‑1 (MQ=255)
                                                                                    gTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                                    >  1:775514/1‑148 (MQ=255)
                                                                                     ttCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                >  1:1046390/1‑151 (MQ=255)
                                                                                      tCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                >  1:1189064/1‑150 (MQ=255)
                                                                                      tCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                >  1:320160/1‑150 (MQ=255)
                                                                                         aaatccgccccccGTT‑CGCACCCCGGTTTTGCCTTCG‑CTTTAAAAAG‑CCCGGTTTTT‑ACCC‑CT‑TTTATCCCCAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                       <  1:619617/130‑1 (MQ=255)
                                                                                            a‑CGGCCCCCGTT‑CGAAACCGGGTTTTGTCTCCCCCATTAAAAAG‑CAAGT‑TTTT‑ACCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGa                                                          <  2:931902/150‑1 (MQ=255)
                                                                                               ggcccccGTC‑CACAATCGGGGTTTGGCTTCCCCATAAAAAAG‑CCGGT‑TTTT‑ACCC‑CT‑TTTATACAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaaa                                                        <  1:952850/145‑1 (MQ=255)
                                                                                                acccccttt‑CCCATTCCTGTTTTGCCTTCGCAATAAAAAAC‑CGAGT‑TTTT‑ACCC‑CT‑TTTATCCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAAc                                                       <  1:759224/143‑1 (MQ=255)
                                                                                                    ccttt‑CACAATCCGGTTTTGCCTTCGCCATAAATAAC‑CCAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                     <  2:1092975/145‑1 (MQ=255)
                                                                                                    ccGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                                  >  1:642887/1‑151 (MQ=255)
                                                                                                    ccGTC‑CGCAACCTTGGTTGGGCTTCACCTTTAACAGC‑CCAGT‑TTTT‑ACCG‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                    <  2:592662/149‑1 (MQ=255)
                                                                                                     cctttccccccccTGTTTTGGCTCCCCAATAAAAAAC‑CCGGT‑TTTT‑CACC‑CC‑TTTATCCCCAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                    <  1:344496/138‑1 (MQ=255)
                                                                                                      gTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                 >  2:853643/1‑150 (MQ=255)
                                                                                                       tt‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                >  1:706747/1‑150 (MQ=255)
                                                                                                       tt‑CACAATCCTGTTTGGGCTTCGCAATAAATAAG‑CAAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt                                                   <  2:879266/147‑1 (MQ=255)
                                                                                                        tccgcacccgtgttttGCCTCCGCCTTAAAAAGG‑CCTGG‑TTTT‑‑TCC‑GC‑TTTTTTCCCAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAACCTTATTga                                                <  1:686411/137‑1 (MQ=255)
                                                                                                          cccAATCCTGTTTTGGCTTCCCCATTAAAAAG‑CCAGT‑TTTT‑ATCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                             <  2:704664/149‑1 (MQ=255)
                                                                                                          cGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCAAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                >  2:625660/1‑148 (MQ=255)
                                                                                                             aaTCCTGTTTTGGCTCCCCCATAAATAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTATACACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaag                                              <  2:622340/147‑1 (MQ=255)
                                                                                                             aaTCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                          >  1:37631/1‑151 (MQ=255)
                                                                                                                ccTGTTTGGGTTTCGCAATTAATAAC‑CCAGG‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                         <  2:821160/149‑1 (MQ=255)
                                                                                                                  tGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtct                                     >  1:323140/1‑151 (MQ=255)
                                                                                                                     tttGTCTTCGCCATAAAAAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTAATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                            <  1:1157129/141‑1 (MQ=255)
                                                                                                                     tttGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                  >  2:208993/1‑151 (MQ=255)
                                                                                                                     ttgggCTTCGCAATAAATAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTATACACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                    <  2:1199627/146‑1 (MQ=255)
                                                                                                                       tGGCCTCCCCAATAAAAAA‑CCCGT‑TTTT‑AACCCCC‑TTTTTCCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                  <  1:186257/150‑1 (MQ=255)
                                                                                                                        gtcTTCGCCATAAAAAAG‑CCGGG‑TTTT‑ACCC‑CC‑TTTATCCAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                  <  1:615171/146‑1 (MQ=255)
                                                                                                                        gccTTCGCCATTAATAGC‑CCATG‑TTTC‑ATCC‑CT‑TTTACCCAACC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                               <  1:835338/149‑1 (MQ=255)
                                                                                                                         ggttCCCCCTTAAATAG‑GCAGG‑TTTTAATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTg                                 <  1:375345/147‑1 (MQ=255)
                                                                                                                           ttCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                            >  1:760134/1‑151 (MQ=255)
                                                                                                                           ttCGCCATAAAAAGG‑CGAGT‑TTTT‑ACCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt                              <  1:324129/149‑1 (MQ=255)
                                                                                                                            tccccATTAAAAAACCCAGT‑TTTT‑ATCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                               <  1:952932/145‑1 (MQ=255)
                                                                                                                               caaTTAAAAGG‑CAAGT‑TTTA‑ATCC‑CC‑TTGATACAAAT‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                         <  2:853715/148‑1 (MQ=255)
                                                                                                                                  ttAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                      >  2:681105/1‑150 (MQ=255)
                                                                                                                                   taataaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                     >  2:801727/1‑150 (MQ=255)
                                                                                                                                     ataaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt                  >  1:430398/1‑151 (MQ=255)
                                                                                                                                     aaaaacccAGT‑TTTT‑ATCC‑CT‑TTTAACCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                       <  1:17728/141‑1 (MQ=255)
                                                                                                                                      aaaacgccatttttt‑ATCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                    <  1:813309/139‑1 (MQ=255)
                                                                                                                                       aaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt                  >  1:1103314/1‑149 (MQ=255)
                                                                                                                                            cAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg              >  2:850047/1‑149 (MQ=255)
                                                                                                                                            cAGG‑GTTT‑ATCA‑CA‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg              >  1:126640/1‑149 (MQ=255)
                                                                                                                                               gttttt‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAg            <  2:1020446/147‑1 (MQ=255)
                                                                                                                                               g‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt         >  1:1006976/1‑151 (MQ=255)
                                                                                                                                                 tttt‑ACCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa          <  2:347930/149‑1 (MQ=255)
                                                                                                                                                  tttactCA‑CT‑TTTTCTCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCATCTGCTTTTTTGTCAAAATAGTGAg            <  2:120166/142‑1 (MQ=255)
                                                                                                                                                   tt‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg       <  2:691956/150‑1 (MQ=255)
                                                                                                                                                    t‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt     >  1:1111237/1‑151 (MQ=255)
                                                                                                                                                         a‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt  >  1:883103/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                        
CCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTT  >  NC_000913/4537962‑4538258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑