Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,1170TC42.0% 126.1 / 49.2 104N217D (AAT→GAT) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base T (45/13);  new base C (0/42);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 5.90e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

AAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTAT  >  NC_000913/4537968‑4538264
                                                                                                                                                             |                                                                                                                                                    
aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTAtt                                                                                                                                                      >  2:585405/1‑150 (MQ=255)
 aaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTc                                                                                                                                                     >  1:127028/1‑150 (MQ=255)
  aaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTcac                                                                                                                                                   <  1:70912/151‑1 (MQ=255)
       ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccg                                                                                                                                             <  1:46033/151‑1 (MQ=255)
       ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccg                                                                                                                                             <  2:931036/151‑1 (MQ=255)
       ccAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑cc                                                                                                                                              <  1:441088/150‑1 (MQ=255)
         aGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑ccgcc                                                                                                                                           >  1:115052/1‑151 (MQ=255)
             aCGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCAc                                                                                                                                       >  2:751546/1‑151 (MQ=255)
             aCGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCAc                                                                                                                                       <  2:694378/151‑1 (MQ=255)
                 cATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGc                                                                                                                                   >  1:7457/1‑151 (MQ=255)
                   tcgggTGATGAGCGGGGAGCAATTTTTTTCAATTTTAAATTATTCCGGGT‑AAAATAAAGTTCAAATC‑CGGCCCCCGTC‑CGCAATCCTGGTTTGGCTTCGCCATAAATAAG‑CCAGG‑TTTT‑ATCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGc                                                                                                                                   <  1:110230/146‑1 (MQ=255)
                      ggggatgaGACGGGAACAAGTTTTTCCCTTTTTAAATTTTTCCCGTT‑AAAATAAAGTTAAAATC‑CGGCATCCGTT‑CCCAATCCTGTTTTGGCTCCGCCATTAAAAAC‑CCAGT‑TTTT‑ATCC‑CT‑TTTTTCCACAA‑CCGCCGCACCAGCCg                                                                                                                                 <  1:722129/145‑1 (MQ=255)
                          atggGACGGGAGCAAGTTTTTTCCTTTTAAAATTTTTCCGGGT‑AAAAAAAAGTTAAAATC‑CGCCCCCCTTC‑CGAAATCCTGGTTGGCCTCCGCCTTAAAAAGG‑CCGGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGt                                                                                                                           <  1:146901/150‑1 (MQ=255)
                           tgaGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTAc                                                                                                                         >  1:795023/1‑151 (MQ=255)
                                  ggAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAg                                                                                                                      >  2:630943/1‑147 (MQ=255)
                                       aaGTTTATTCCATTTAAAATTATTCCCGGT‑AAAATCAATTTCAAATA‑CGGCATCCGTT‑CGCAACCCTGGTTTGGCTTCCCCATTAATAAG‑CCGGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTc                                                                                                              <  2:818752/150‑1 (MQ=255)
                                        aGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                            >  1:1174796/1‑151 (MQ=255)
                                        aGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGc                                                                                                            >  1:863117/1‑151 (MQ=255)
                                         gCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                           >  1:331453/1‑151 (MQ=255)
                                           ttATTCCTTTTTAATTTATTCCGGGT‑AAAATCAATTTCAATTA‑CGGCATCCGTT‑CGCAACCCTGTTTTGGCTCCCCCTTTAAAAAC‑CCAGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGcc                                                                                                           <  2:790439/149‑1 (MQ=255)
                                              ttCTATTTAAAATTATT‑CCGGTAAAAATAAAGTTAAAATC‑CGCCCCCCTTC‑CGCACCCTTGTTTTGGCTCCCCCTTAAATACC‑CCGGT‑TTTT‑ACCC‑CC‑TTTTCCCCAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                         <  1:655913/148‑1 (MQ=255)
                                              ttCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                      >  1:464753/1‑151 (MQ=255)
                                              ttCCATTTAAAATTATTCCCGGT‑AAAATCAGGTTAAAATC‑CGGCATCCGTT‑CGAAATCCTGTTTTGGTTCCGCAATAAAAAAG‑CCGGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGc                                                                                                        <  2:21509/149‑1 (MQ=255)
                                              tcctaTTTTAATTTATTCCCGTT‑AAATTAATTTTCAAATA‑CGGCTTCCTTT‑CGCAATCTGGGTTTGTTTTCCCAATTAATAAG‑CCGGT‑TTTT‑ATCT‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                         <  2:534026/144‑1 (MQ=255)
                                               tCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGt                                                                                                      >  2:337421/1‑150 (MQ=255)
                                                   tttaAAATTATCCCCGGG‑GAAAGCAGCTACAAATC‑CGCCATCCTTT‑CCAAATCCTGGTTTGCCTCCGCCATTAATAAG‑CCGGG‑TTTT‑TTCC‑CT‑TTTATCCACAG‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAg                                                                                                         <  2:259668/143‑1 (MQ=255)
                                                    tttAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                                >  2:1148544/1‑151 (MQ=255)
                                                    tttAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTcc                                                                                                >  1:998046/1‑151 (MQ=255)
                                                       aatttatcccgggT‑AAAACCAAGTTAAAATC‑CGGCATCCTTT‑CGAATCCCTGTTTTGGTTTCGCCATTAAAAAG‑CCAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                             <  2:664430/140‑1 (MQ=255)
                                                       aaaTTATTCCCGTT‑AAAATCAAGTAAAATTA‑CGCCACCCGTC‑CGCAATCTTGGTTTGGCTTCCCCATTAATAAC‑CCGGT‑TTTT‑ACCC‑CC‑TTTATCCAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                             <  2:321176/151‑1 (MQ=255)
                                                       aaaTTATTACGGGT‑AAAATAAATTTAAAATA‑CGCCATCCTTT‑CGCAATCCTGTTTGGGCTCCGCCATAAATAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                                             <  2:793566/151‑1 (MQ=255)
                                                       aaaTTATCCCGGGT‑AAAATCAA‑TTTAAATC‑CCGCCCCCGTTCCCCAACCCTGGTTTGCTTTCCCAATAAAAAAG‑CC‑GG‑TTTT‑AACC‑CCTTTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCa                                                                                               <  1:1008475/149‑1 (MQ=255)
                                                        aaTTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                            >  2:294053/1‑151 (MQ=255)
                                                        aaTTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTg                                                                                            >  2:690750/1‑151 (MQ=255)
                                                         attattACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGc                                                                                           >  2:619638/1‑151 (MQ=255)
                                                              taCCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTgtgt                                                                                      >  1:616417/1‑151 (MQ=255)
                                                               aCCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                                     >  2:493911/1‑151 (MQ=255)
                                                                ccGGT‑AAAATAAAGTTAAAATA‑CGGCATCCTTT‑CGCAATCCTGGTTTGTCTCCGCCATAAAAAAG‑CCGGT‑TTTT‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                                     <  1:1014424/150‑1 (MQ=255)
                                                                 cGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                                    >  1:863168/1‑150 (MQ=255)
                                                                   gT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAgg                                                                                 >  2:36878/1‑151 (MQ=255)
                                                                    t‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGACTTCGCCATTAATAAG‑CCAGG‑CTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGtt                                                                                     >  1:520794/1‑146 (MQ=255)
                                                                      gAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAggg                                                                                >  2:1154208/1‑150 (MQ=255)
                                                                       aaaTCAAGTTAAAATC‑CGGCATCCTTT‑CGCAATCCTGTTTTGGCTTCGCCATTAATAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                              <  2:128877/151‑1 (MQ=255)
                                                                       aaaTCAAGTTAAAATA‑CGGCACCCGTT‑CGCAATCCTGGTTTGGTTTCGCCATTAATAAG‑CCAGT‑TTTT‑ACCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                              <  2:181376/151‑1 (MQ=255)
                                                                        aaaccaattCAAAAACCGGCATCCGTT‑CGCATCCCGGGTTTGCCTCCGCCATAAAAAAC‑CCAGG‑TTTA‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGAt                                                                              <  1:616292/144‑1 (MQ=255)
                                                                         acaaatttaaactA‑CGGCACCCGTC‑CGCAACCCTGTTTGGGCTCCGCCATAAAAAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTACCCCAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                             <  1:337987/138‑1 (MQ=255)
                                                                           aaattaaaaaTG‑CGGCACCCGTT‑CCCAACCCGGTTTTGTCTTCCCCATTAAAAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaa                                                                           <  1:1154759/143‑1 (MQ=255)
                                                                              gTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                                          >  1:775514/1‑148 (MQ=255)
                                                                               ttCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                      >  1:1046390/1‑151 (MQ=255)
                                                                                tCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                      >  1:1189064/1‑150 (MQ=255)
                                                                                tCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGac                                                                      >  1:320160/1‑150 (MQ=255)
                                                                                   aaatccgccccccGTT‑CGCACCCCGGTTTTGCCTTCG‑CTTTAAAAAG‑CCCGGTTTTT‑ACCC‑CT‑TTTATCCCCAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                                             <  1:619617/130‑1 (MQ=255)
                                                                                      a‑CGGCCCCCGTT‑CGAAACCGGGTTTTGTCTCCCCCATTAAAAAG‑CAAGT‑TTTT‑ACCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGa                                                                <  2:931902/150‑1 (MQ=255)
                                                                                         ggcccccGTC‑CACAATCGGGGTTTGGCTTCCCCATAAAAAAG‑CCGGT‑TTTT‑ACCC‑CT‑TTTATACAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaaa                                                              <  1:952850/145‑1 (MQ=255)
                                                                                          acccccttt‑CCCATTCCTGTTTTGCCTTCGCAATAAAAAAC‑CGAGT‑TTTT‑ACCC‑CT‑TTTATCCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAAc                                                             <  1:759224/143‑1 (MQ=255)
                                                                                              ccttt‑CACAATCCGGTTTTGCCTTCGCCATAAATAAC‑CCAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACtt                                                           <  2:1092975/145‑1 (MQ=255)
                                                                                              ccGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAtt                                                        >  1:642887/1‑151 (MQ=255)
                                                                                              ccGTC‑CGCAACCTTGGTTGGGCTTCACCTTTAACAGC‑CCAGT‑TTTT‑ACCG‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                          <  2:592662/149‑1 (MQ=255)
                                                                                               cctttccccccccTGTTTTGGCTCCCCAATAAAAAAC‑CCGGT‑TTTT‑CACC‑CC‑TTTATCCCCAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTa                                                          <  1:344496/138‑1 (MQ=255)
                                                                                                gTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTg                                                       >  2:853643/1‑150 (MQ=255)
                                                                                                 tt‑CACAATCCTGTTTGGGCTTCGCAATAAATAAG‑CAAGT‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTAt                                                         <  2:879266/147‑1 (MQ=255)
                                                                                                  tccgcacccgtgttttGCCTCCGCCTTAAAAAGG‑CCTGG‑TTTT‑‑TCC‑GC‑TTTTTTCCCAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAACCTTATTga                                                      <  1:686411/137‑1 (MQ=255)
                                                                                                    cccAATCCTGTTTTGGCTTCCCCATTAAAAAG‑CCAGT‑TTTT‑ATCC‑CT‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                                   <  2:704664/149‑1 (MQ=255)
                                                                                                    cGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCAAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTga                                                      >  2:625660/1‑148 (MQ=255)
                                                                                                       aaTCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaat                                                >  1:37631/1‑151 (MQ=255)
                                                                                                          ccTGTTTGGGTTTCGCAATTAATAAC‑CCAGG‑TTTT‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAaata                                               <  2:821160/149‑1 (MQ=255)
                                                                                                               tttGTCTTCGCCATAAAAAAG‑CCAGT‑TTTT‑ATCC‑CC‑TTAATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaagaa                                                  <  1:1157129/141‑1 (MQ=255)
                                                                                                               tttGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                        >  2:208993/1‑151 (MQ=255)
                                                                                                               ttgggCTTCGCAATAAATAAG‑CCAGT‑TTTT‑ACCC‑CC‑TTTATACACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtctt                                          <  2:1199627/146‑1 (MQ=255)
                                                                                                                 tGGCCTCCCCAATAAAAAA‑CCCGT‑TTTT‑AACCCCC‑TTTTTCCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                        <  1:186257/150‑1 (MQ=255)
                                                                                                                  gtcTTCGCCATAAAAAAG‑CCGGG‑TTTT‑ACCC‑CC‑TTTATCCAAAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAAtcttct                                        <  1:615171/146‑1 (MQ=255)
                                                                                                                  gccTTCGCCATTAATAGC‑CCATG‑TTTC‑ATCC‑CT‑TTTACCCAACC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                                     <  1:835338/149‑1 (MQ=255)
                                                                                                                   ggttCCCCCTTAAATAG‑GCAGG‑TTTTAATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTg                                       <  1:375345/147‑1 (MQ=255)
                                                                                                                     ttCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                                  >  1:760134/1‑151 (MQ=255)
                                                                                                                     ttCGCCATAAAAAGG‑CGAGT‑TTTT‑ACCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt                                    <  1:324129/149‑1 (MQ=255)
                                                                                                                      tccccATTAAAAAACCCAGT‑TTTT‑ATCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCt                                     <  1:952932/145‑1 (MQ=255)
                                                                                                                         caaTTAAAAGG‑CAAGT‑TTTA‑ATCC‑CC‑TTGATACAAAT‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg                               <  2:853715/148‑1 (MQ=255)
                                                                                                                            ttAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCa                            >  2:681105/1‑150 (MQ=255)
                                                                                                                             taataaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaa                           >  2:801727/1‑150 (MQ=255)
                                                                                                                               ataaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt                        >  1:430398/1‑151 (MQ=255)
                                                                                                                               aaaaacccAGT‑TTTT‑ATCC‑CT‑TTTAACCAAAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTc                             <  1:17728/141‑1 (MQ=255)
                                                                                                                                aaaacgccatttttt‑ATCC‑CT‑TTTTTCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCaaa                          <  1:813309/139‑1 (MQ=255)
                                                                                                                                 aaG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt                        >  1:1103314/1‑149 (MQ=255)
                                                                                                                                      cAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg                    >  2:850047/1‑149 (MQ=255)
                                                                                                                                      cAGG‑GTTT‑ATCA‑CA‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg                    >  1:126640/1‑149 (MQ=255)
                                                                                                                                         gttttt‑ATCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAg                  <  2:1020446/147‑1 (MQ=255)
                                                                                                                                         g‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCAt               >  1:1006976/1‑151 (MQ=255)
                                                                                                                                           tttt‑ACCC‑CT‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCa                <  2:347930/149‑1 (MQ=255)
                                                                                                                                             tt‑ATCC‑CC‑TTTATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg             <  2:691956/150‑1 (MQ=255)
                                                                                                                                              t‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAt           >  1:1111237/1‑151 (MQ=255)
                                                                                                                                                taaccccttttATCCACAT‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTg             <  1:1079117/142‑1 (MQ=255)
                                                                                                                                                   a‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt        >  1:883103/1‑150 (MQ=255)
                                                                                                                                                    cccttttCTTCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt        <  1:435845/147‑1 (MQ=255)
                                                                                                                                                    cccttttATCCACAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtt          <  2:199973/145‑1 (MQ=255)
                                                                                                                                                    cccttttATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGa            <  2:47789/143‑1 (MQ=255)
                                                                                                                                                      c‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATc     >  1:294744/1‑151 (MQ=255)
                                                                                                                                                         ttATCCCCAC‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCt    <  2:331467/150‑1 (MQ=255)
                                                                                                                                                         ttATCCAAAT‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGAtttt        <  2:338954/146‑1 (MQ=255)
                                                                                                                                                          tATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCtat  >  1:381243/1‑151 (MQ=255)
                                                                                                                                                          tATCCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCta   <  2:493802/150‑1 (MQ=255)
                                                                                                                                                             |                                                                                                                                                    
AAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGT‑GAAATCAAGTTCAAATA‑CGGCATCCGTT‑CGCAATCCTGGTTTGGCTTCGCCATTAATAAG‑CCAGG‑TTTT‑ATCA‑CC‑TTTATTCACAA‑CCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTGAGCATTGATTTTATCTAT  >  NC_000913/4537968‑4538264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑