Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,025	G→A	R209H (CGT→CAT)	spoT →	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,025	0	G	A	100.0%	259.0 / NA	65	R209H (CGT→CAT)	spoT	bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/35);  total (30/35)

CGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCAT  >  NC_000913/3822877‑3823172                                                                                                                                                     |                                                                                                                                                    cGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATa                                                                                                                                                   <  2:558068/151‑1 (MQ=255) cGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATa                                                                                                                                                   <  1:305922/151‑1 (MQ=255)          ctcGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAgat                                                                                                                                            <  2:338754/149‑1 (MQ=255)            cGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAgatgat                                                                                                                                         <  1:162712/150‑1 (MQ=255)                   tataGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCagaag                                                                                                                                  <  1:410595/150‑1 (MQ=255)                    ataGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCagaaga                                                                                                                                 <  2:231395/150‑1 (MQ=255)                       gCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGAttct                                                                                                                             >  2:773358/1‑151 (MQ=255)                        cccGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGAttctt                                                                                                                            <  1:648131/151‑1 (MQ=255)                        cccGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGAttctt                                                                                                                            >  2:69787/1‑151 (MQ=255)                          cGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGAttctttc                                                                                                                          <  1:463905/151‑1 (MQ=255)                             tGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTg                                                                                                                        >  2:225957/1‑150 (MQ=255)                              ggCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGaa                                                                                                                      <  1:667438/151‑1 (MQ=255)                                  cACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATc                                                                                                                   <  2:289464/150‑1 (MQ=255)                                   aCCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCg                                                                                                                  >  2:325846/1‑150 (MQ=255)                                        ttAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAggg                                                                                                             <  1:448478/150‑1 (MQ=255)                                         tAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCg                                                                                                           >  2:313465/1‑151 (MQ=255)                                          aGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGt                                                                                                          >  2:387590/1‑151 (MQ=255)                                           ggTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGtt                                                                                                         <  2:559119/151‑1 (MQ=255)                                           ggTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGtt                                                                                                         <  2:840526/151‑1 (MQ=255)                                              aTCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTg                                                                                                       <  2:1086170/150‑1 (MQ=255)                                              aTCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTg                                                                                                       <  1:1055332/150‑1 (MQ=255)                                                       aTTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGAGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGGGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGACATCGAAGGGCGGTTGCAGGAAGCgg                                                                                             >  1:989543/1‑151 (MQ=255)                                                            aaCCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATa                                                                                        <  1:184889/151‑1 (MQ=255)                                                              ccGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATAcc                                                                                      >  2:1038790/1‑151 (MQ=255)                                                              ccGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATAcc                                                                                      <  2:675259/151‑1 (MQ=255)                                                                   cTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGcc                                                                                 >  1:64027/1‑151 (MQ=255)                                                                     cGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCcgc                                                                               >  1:193117/1‑151 (MQ=255)                                                                     cGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCcgc                                                                               >  2:88169/1‑151 (MQ=255)                                                                      gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCcgcg                                                                              >  1:1092395/1‑151 (MQ=255)                                                                        agagCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTc                                                                            >  1:700549/1‑151 (MQ=255)                                                                         gagCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCa                                                                           <  2:1039546/151‑1 (MQ=255)                                                                          agCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTc                                                                            >  2:119659/1‑149 (MQ=255)                                                                          agCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAg                                                                          <  1:46388/151‑1 (MQ=255)                                                                              gggTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTgg                                                                       <  1:1071548/150‑1 (MQ=255)                                                                                   ttGAGGTGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTcgcg                                                                  >  2:956523/1‑150 (MQ=255)                                                                                     gAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCgaga                                                               <  1:190310/151‑1 (MQ=255)                                                                                      aGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTTGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCgatag                                                              <  1:1159777/151‑4 (MQ=255)                                                                                         cgcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGc                                                            >  1:549479/1‑150 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCAt                                                          <  2:459249/151‑1 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCAt                                                          >  1:1174448/1‑151 (MQ=255)                                                                                            tGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGTTGATCCAGAAGATTCTTCCTGAAATCGAAGGGGGTTTGCAGGAAGCGGGAATACCGTGCCGCGCCAGTGGTCGCGAGAAGCATCt                                                        >  2:933837/1‑151 (MQ=255)                                                                                            tGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCt                                                        >  1:860392/1‑151 (MQ=255)                                                                                             gTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCt                                                        <  1:384439/150‑1 (MQ=255)                                                                                                tCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTAt                                                    >  2:1112066/1‑151 (MQ=255)                                                                                                tCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTAt                                                    >  2:1120990/1‑151 (MQ=255)                                                                                                    aaCCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCg                                                 >  2:1014652/1‑150 (MQ=255)                                                                                                      ccGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGAtt                                              >  2:1073692/1‑151 (MQ=255)                                                                                                      ccGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGAtt                                              <  2:507898/151‑1 (MQ=255)                                                                                                       cGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGAttt                                             <  1:607606/151‑1 (MQ=255)                                                                                                       cGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGAttt                                             <  2:64436/151‑1 (MQ=255)                                                                                                        gTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTa                                            <  1:168401/151‑1 (MQ=255)                                                                                                           aTCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACt                                          <  2:1172543/150‑1 (MQ=255)                                                                                                           aTCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTg                                         >  1:705038/1‑151 (MQ=255)                                                                                                             cgcgTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGc                                        >  1:1138741/1‑150 (MQ=255)                                                                                                               cgTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCaaa                                     <  1:801236/151‑1 (MQ=255)                                                                                                                gTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCaaaa                                    <  1:193107/151‑1 (MQ=255)                                                                                                                gTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCaaaa                                    <  1:227178/151‑1 (MQ=255)                                                                                                                     cAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGt                                <  2:1010572/150‑1 (MQ=255)                                                                                                                     cAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGCGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGt                                <  2:743186/150‑1 (MQ=255)                                                                                                                         gaagTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTc                            >  1:594841/1‑150 (MQ=255)                                                                                                                          aagTGGTGAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCa                           >  1:761698/1‑150 (MQ=255)                                                                                                                                tgAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGagc                     >  1:1075891/1‑150 (MQ=255)                                                                                                                                 gAAAGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGagca                    <  2:1066047/150‑1 (MQ=255)                                                                                                                                    aGCCGCGCGCGGCAACCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGt                >  1:1010704/1‑151 (MQ=255)                                                                                                                                                  aCCATAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCAt  >  2:1137508/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCAT  >  NC_000913/3822877‑3823172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑