Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,257,602 A→T N163I (AAT→ATT)  lexA → transcriptional repressor of SOS regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,257,6020AT100.0% 290.4 / NA 75N163I (AAT→ATT) lexAtranscriptional repressor of SOS regulon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (32/43);  total (32/43)

GCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCC  >  NC_000913/4257455‑4257746
                                                                                                                                                    |                                                                                                                                                
gcgTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTaa                                                                                                                                               <  1:911422/151‑1 (MQ=255)
  gTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAg                                                                                                                                             <  1:417210/151‑1 (MQ=255)
  gTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAg                                                                                                                                             <  1:1043714/151‑1 (MQ=255)
   tCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGt                                                                                                                                            >  2:1189049/1‑151 (MQ=255)
        gggATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAAc                                                                                                                                       >  1:1092524/1‑151 (MQ=255)
             gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTATCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTg                                                                                                                                  <  1:288834/151‑1 (MQ=255)
             gTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGtt                                                                                                                                   <  1:646133/150‑1 (MQ=255)
              tCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGc                                                                                                                                 >  2:852214/1‑151 (MQ=255)
               cGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGc                                                                                                                                 <  1:850981/150‑1 (MQ=255)
                 aTGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGcc                                                                                                                                >  1:753129/1‑149 (MQ=255)
                  tGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGcc                                                                                                                                >  1:961921/1‑148 (MQ=255)
                    aaaGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGaaa                                                                                                                           <  2:751340/151‑1 (MQ=255)
                      aGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAAt                                                                                                                         <  1:923214/151‑1 (MQ=255)
                      aGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAAt                                                                                                                         >  2:43553/1‑151 (MQ=255)
                      aGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAAt                                                                                                                         <  2:348048/151‑1 (MQ=255)
                        atatCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAAt                                                                                                                         >  1:186221/1‑149 (MQ=255)
                            cGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCg                                                                                                                     >  1:790349/1‑149 (MQ=255)
                            cGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGa                                                                                                                    <  1:333377/150‑1 (MQ=255)
                              gCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGtt                                                                                                                 <  2:186207/151‑1 (MQ=255)
                                aTTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGttt                                                                                                                >  1:111058/1‑150 (MQ=255)
                                aTTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTa                                                                                                               <  1:1075071/151‑1 (MQ=255)
                                aTTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTa                                                                                                               <  1:894167/151‑1 (MQ=255)
                                  tATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTaa                                                                                                              >  1:1173891/1‑150 (MQ=255)
                                   atggatggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAAc                                                                                                            >  2:647584/1‑151 (MQ=255)
                                    tggatggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAAcc                                                                                                           >  1:296031/1‑151 (MQ=255)
                                         ggTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAAtt                                                                                                       <  1:206099/150‑1 (MQ=255)
                                                tGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGtt                                                                                                 <  2:421921/149‑1 (MQ=255)
                                                tGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTg                                                                                                >  1:420312/1‑150 (MQ=255)
                                                 gCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGAc                                                                                              >  2:766163/1‑151 (MQ=255)
                                                    ggCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCt                                                                                            <  1:226846/150‑1 (MQ=255)
                                                     gCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTc                                                                                          <  2:846404/151‑1 (MQ=255)
                                                            aTAAAACTCAGGATGTACGTAACGGTCAGGCCGTT‑TCGCCAGGAATTATTGAGGAGGTTACGGTAAAGC‑CCTTGAAAACCAGGGCTTTAAAGCCGAATTGTTGCAAGAAAAAACGGAGTTTAAA‑CAATTggccgtggcctt                                                                                           >  2:651135/1‑130 (MQ=255)
                                                             tAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTcagc                                                                                    >  1:183364/1‑149 (MQ=255)
                                                                     aGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCAc                                                                          >  2:978654/1‑151 (MQ=255)
                                                                         tGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCAtt                                                                      >  1:658098/1‑151 (MQ=255)
                                                                              gTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAgg                                                                 <  1:662566/151‑1 (MQ=255)
                                                                                 aCGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCt                                                              <  1:211132/151‑1 (MQ=255)
                                                                                  cGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTg                                                             <  1:772986/151‑1 (MQ=255)
                                                                                       aGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCgg                                                         >  2:940608/1‑150 (MQ=255)
                                                                                       aGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGt                                                        <  1:394874/151‑1 (MQ=255)
                                                                                        ggTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCCCCATTGAAGGGCTTGCGGtt                                                       <  2:951705/151‑1 (MQ=255)
                                                                                        ggTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGtt                                                       <  2:747522/151‑1 (MQ=255)
                                                                                           cGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTgg                                                     <  2:75369/150‑1 (MQ=255)
                                                                                            gTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTgggg                                                   >  1:952885/1‑151 (MQ=255)
                                                                                                 cG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTAt                                               <  1:835813/150‑1 (MQ=255)
                                                                                                    cACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTAtt                                              >  2:903771/1‑149 (MQ=255)
                                                                                                         aTTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAAc                                        <  2:287929/150‑1 (MQ=255)
                                                                                                            gatgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACgg                                      >  2:622637/1‑149 (MQ=255)
                                                                                                             atgaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGa                                   <  1:361217/151‑1 (MQ=255)
                                                                                                               gaCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACt                                 <  1:94961/151‑1 (MQ=255)
                                                                                                                aCGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTg                                <  1:1003342/151‑1 (MQ=255)
                                                                                                                   aaGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCATTTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTggat                             <  1:707474/151‑3 (MQ=255)
                                                                                                                   aaGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCt                             <  2:186232/151‑1 (MQ=255)
                                                                                                                    aGTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTTTTCGCAACGGCGACTGGCt                             >  1:346212/1‑150 (MQ=255)
                                                                                                                     gTTACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTgg                               >  2:591025/1‑147 (MQ=255)
                                                                                                                       tACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTa                          <  1:1032297/150‑1 (MQ=255)
                                                                                                                       tACCGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTa                          <  1:560806/150‑1 (MQ=255)
                                                                                                                         ccGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACa                       <  2:1173934/151‑1 (MQ=255)
                                                                                                                          cGTTAAGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACa                       >  2:34577/1‑150 (MQ=255)
                                                                                                                              aaGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTTGCAACGGCGACTGGCTGTAACATAtct                  >  2:610192/1‑151 (MQ=255)
                                                                                                                               aGCGCCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATAtctc                 <  2:1197794/151‑1 (MQ=255)
                                                                                                                                 cgcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATctct                >  1:160909/1‑150 (MQ=255)
                                                                                                                                  gcCTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTTGCAACGGCCAATGGCTGTAACa                       >  1:813433/1‑142 (MQ=255)
                                                                                                                                    cTGAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTCGCAACGGCGACTGGCTGTAACATATctct                >  2:1099726/1‑147 (MQ=255)
                                                                                                                                      gAAAAAACAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGAcc          <  1:314364/151‑1 (MQ=255)
                                                                                                                                        aaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGGGGGAACATATCTTTGAGACcg         >  2:653409/1‑150 (MQ=255)
                                                                                                                                        aaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACcgc        <  1:91293/151‑1 (MQ=255)
                                                                                                                                        aaaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACcgc        <  2:9323/151‑1 (MQ=255)
                                                                                                                                         aaaaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACcgcg       <  2:794965/151‑1 (MQ=255)
                                                                                                                                           aaCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGAt     <  2:753153/151‑1 (MQ=255)
                                                                                                                                            aCAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATg    <  2:1111725/151‑1 (MQ=255)
                                                                                                                                             cAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGAt     >  2:971082/1‑149 (MQ=255)
                                                                                                                                             cAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgc   <  1:31480/151‑1 (MQ=255)
                                                                                                                                             cAGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGGTATTTGCAAAGGCGACTGGCTGGAAAATATCTCTGAGACcgcg       >  2:1138906/1‑147 (MQ=255)
                                                                                                                                              aGGGCATTAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATgcc  <  1:287989/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCGTCAGCGGGATGTCGATGAAAGATATCGGCATTATGGATGGTGACTTGCTGGCAGTGCATAAAACTCAGGATGTACGTAACGGTCAGGTCGTTGTCG‑CACGTATTGATGACGAAGTTACCGTTAAGCGCCTGAAAAAACAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCC  >  NC_000913/4257455‑4257746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑